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The map label for this gene is fruB [H]

Identifier: 238895726

GI number: 238895726

Start: 3658500

End: 3659630

Strand: Reverse

Name: fruB [H]

Synonym: KP1_3829

Alternate gene names: 238895726

Gene position: 3659630-3658500 (Counterclockwise)

Preceding gene: 238895728

Following gene: 238895725

Centisome position: 69.73

GC content: 62.86

Gene sequence:

>1131_bases
ATGTTCCAGTTATCTGTGCAGGATATTCATCCCGGCCAGCAGGCCGGTAATAAAGAAGAGGCCATCCGGCAGGTCGCCGC
AGCGCTGGTCAGCGCCGGCAACGTGGCCGATGGCTACGTGAACGGCATGCTGGCGCGCGAGCAGCAGACCTCGACCTTCC
TCGGCAACGGGATTGCGATCCCGCACGGCACGACGGATACGCGTGACCAGGTGCTGAAGACCGGCGTTCAGGTGTTTCAG
TTCCCGCAGGGCGTCACCTGGGGTGAAGGGCAGACCGCCTATGTGGCGATCGGGATTGCCGCCAGCTCAGATGAACACCT
CGGCCTGCTGCGTCAGCTGACCCACGTGCTGAGCGATGACGCGGTCGCCGCGCAGCTGCAGTCCGCCACCACCGCGGAAG
AGCTGCGTGCGCTGCTGATGGGAGAGAAGCAGAGCGAAGCGCTGAAGCTGGATAACGAGACCCTCAGTCTCGACGTCGCG
GCCAGCGATCTGCTGACCCTGCAGGCGCTGAACGCGGCGCGTCTGAAAGAAGTTGGCGCTGTCGATGCCGCCTTCGTTAG
CCACGTCATTAACGATACGCCGCTGAACCTCGGTCAGGGCGTCTGGCTGAACGACAGTGCGGAAGGCAATTTGCGCAGCG
CCGTGGCGGTCAGCCGCGCGGCTAACGCCTTTACCCGCGACGAGCAGCCGGTTTCCCTGCTGGTCACGGTGGCGATGGCC
GACGAGCAGCCGACCGCAGTGCTGAACCGTCTGAGCAAGCTGCTGCTGGACAAGAAAGCTGAACATTTGCTGAAAGCCGA
CGCCGCCACCGTGCTGGCGCTGCTGACCAGCGACGACGCCATTGCCGAAGATGTGCTGAGCGCCGAATTCGTGGTGCGCA
ATGAACACGGCCTGCATGCCCGTCCGGGCACCATGCTGGTCAACACCATTAAACAATTTTCCAGCGACATCACCGTCACC
AATCTCGACGGTTCCGGCAAGCCGGCTAACGGTCGCAGCCTGATGAAGGTTGTGGCGCTGGGTGTGAAAAAAGGTCACCG
TCTGCGTTTTACCGCGCAGGGTGAGGATGCGCAACAAGCGCTGGATGCGATTGGCGAAGCGATCGCCGCAGGCCTTGGGG
AGGGCGCATAA

Upstream 100 bases:

>100_bases
TTGAAACGTTTCAGCGTCATTCGGCAATCCGTTCCCCGTCAGATGGCGCCTTTTTTCTCAAGTTAGCTGAAACGATTCAA
TTCAGCAGGAGAGGAGAACC

Downstream 100 bases:

>100_bases
ATGAGCAGACGTGTAGCAACGATTACGTTAAACCCGGCATACGACCTGGTGGGATTCACCCCGGAGATTGAACGCGGCGA
AGTGAACCTGGTGCGCACCA

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MFQLSVQDIHPGQQAGNKEEAIRQVAAALVSAGNVADGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPQGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDDAVAAQLQSATTAEELRALLMGEKQSEALKLDNETLSLDVA
ASDLLTLQALNAARLKEVGAVDAAFVSHVINDTPLNLGQGVWLNDSAEGNLRSAVAVSRAANAFTRDEQPVSLLVTVAMA
DEQPTAVLNRLSKLLLDKKAEHLLKADAATVLALLTSDDAIAEDVLSAEFVVRNEHGLHARPGTMLVNTIKQFSSDITVT
NLDGSGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAQQALDAIGEAIAAGLGEGA

Sequences:

>Translated_376_residues
MFQLSVQDIHPGQQAGNKEEAIRQVAAALVSAGNVADGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPQGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDDAVAAQLQSATTAEELRALLMGEKQSEALKLDNETLSLDVA
ASDLLTLQALNAARLKEVGAVDAAFVSHVINDTPLNLGQGVWLNDSAEGNLRSAVAVSRAANAFTRDEQPVSLLVTVAMA
DEQPTAVLNRLSKLLLDKKAEHLLKADAATVLALLTSDDAIAEDVLSAEFVVRNEHGLHARPGTMLVNTIKQFSSDITVT
NLDGSGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAQQALDAIGEAIAAGLGEGA
>Mature_376_residues
MFQLSVQDIHPGQQAGNKEEAIRQVAAALVSAGNVADGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPQGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDDAVAAQLQSATTAEELRALLMGEKQSEALKLDNETLSLDVA
ASDLLTLQALNAARLKEVGAVDAAFVSHVINDTPLNLGQGVWLNDSAEGNLRSAVAVSRAANAFTRDEQPVSLLVTVAMA
DEQPTAVLNRLSKLLLDKKAEHLLKADAATVLALLTSDDAIAEDVLSAEFVVRNEHGLHARPGTMLVNTIKQFSSDITVT
NLDGSGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAQQALDAIGEAIAAGLGEGA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=376, Percent_Identity=85.6382978723404, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=44.1379310344828, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 39504; Mature: 39504

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQLSVQDIHPGQQAGNKEEAIRQVAAALVSAGNVADGYVNGMLAREQQTSTFLGNGIAI
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEC
PHGTTDTRDQVLKTGVQVFQFPQGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDD
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCH
AVAAQLQSATTAEELRALLMGEKQSEALKLDNETLSLDVAASDLLTLQALNAARLKEVGA
HHHHHHHHHCCHHHHHHHHHCCCCHHHEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHCC
VDAAFVSHVINDTPLNLGQGVWLNDSAEGNLRSAVAVSRAANAFTRDEQPVSLLVTVAMA
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEC
DEQPTAVLNRLSKLLLDKKAEHLLKADAATVLALLTSDDAIAEDVLSAEFVVRNEHGLHA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCC
RPGTMLVNTIKQFSSDITVTNLDGSGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAQQA
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
LDAIGEAIAAGLGEGA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFQLSVQDIHPGQQAGNKEEAIRQVAAALVSAGNVADGYVNGMLAREQQTSTFLGNGIAI
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEC
PHGTTDTRDQVLKTGVQVFQFPQGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDD
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCH
AVAAQLQSATTAEELRALLMGEKQSEALKLDNETLSLDVAASDLLTLQALNAARLKEVGA
HHHHHHHHHCCHHHHHHHHHCCCCHHHEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHCC
VDAAFVSHVINDTPLNLGQGVWLNDSAEGNLRSAVAVSRAANAFTRDEQPVSLLVTVAMA
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEC
DEQPTAVLNRLSKLLLDKKAEHLLKADAATVLALLTSDDAIAEDVLSAEFVVRNEHGLHA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCC
RPGTMLVNTIKQFSSDITVTNLDGSGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAQQA
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
LDAIGEAIAAGLGEGA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]