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The map label for this gene is dacD [H]

Identifier: 238895589

GI number: 238895589

Start: 3490943

End: 3492112

Strand: Reverse

Name: dacD [H]

Synonym: KP1_3672

Alternate gene names: 238895589

Gene position: 3492112-3490943 (Counterclockwise)

Preceding gene: 238895591

Following gene: 238895588

Centisome position: 66.54

GC content: 58.97

Gene sequence:

>1170_bases
ATGCCGTTGAAAGGCCGTTTGTTTATTGCTGTATCTTTGCTCGCTTCGAGCGTTTCCTGCGCATTTGCCGTCGACTTACC
TGCTACGGTCGCGCCGCCTTCCATTCAGGCTGGCTCCTGGGTGCTGATGGATTACACCACCGGTCAGGTCCTGACCGCGG
GTAACGAACATCAGCAGCGCAATCCTGCCAGTCTGACCAAACTGATGACCGGCTACGTGGTCGATCGCGCCATCGACAGC
CATCGTATCACCTTTGACGATATCGTCACCGTGGGTAAAGACGCCTGGGCGAAGGGCAACCCGGTGTTCGACGGGTCATC
GCTGATGTTCTTAAAGCCAGGGGATCGCGTCAGCGTTCGCGACCTCAGCCGTGGGCTGATTGTCGACTCCGGTAACGACG
CCTGCGTCGCGCTGGCCGACTATGTCGCCGGCGGCCAGCCGCAGTTTGTCGCCCTGATGAATCAGTATGTCGAAAAACTG
CACCTGCGGGACACCCATTTCGAAACAGTCCACGGCCTCGACGCCCCGGGCCAGCACAGTTCGGCCTATGACCTCGCGGT
GCTCTCGCGGGCGATTATTCATGGCGAACCAGACGTCTACCACATGTACAGCCAGAAAAGCCTGACGTGGAACGGGATCA
CCCAGCAAAACCGTAACGGTCTGCTGTGGGATAAAACGATGAACGTCGATGGCCTGAAAACCGGCCACACCTCGGGGGCC
GGGTTCAACCTGATCGCCTCGGCGGTGGATGGCCAGCGCCGGCTGATCGCCGTGGTGATGGGGGCGGACAGTCCGAAAGG
CCGCGAGCAGCAGGCCGCTAAACTGCTGCACTGGGGGCAGCAGAACTTCGACACCGTGCAGGTGCTGCAGAAGGGGCAGA
AGGTCGGCACCGAGCGTATCTGGTACGGCGATAAAGAGCAGATTAAGCTCGGCACCGATCAGGATTTCTGGTTAGCCCTG
CCGAAGGCGGAAGTGTCGCGCATTAAAGCGAAATATGTGCTGGATAAGAAGGATCTTGAGGCGCCGATCGCCGCCAATCA
GCGGGTTGGCGAGATTTCACTGTATGACGGCGACAAGGTGGTGGGGCACTATCCGCTGGTCACCCTCGAAAGCATCAATA
AAGGCGGAGTGTTCTCGCGAATGAGCGATTACCTGCATCACGAGCTGTAA

Upstream 100 bases:

>100_bases
AAGCAACTTCCTCTGCTGTGTCATATTTTCTTGCAATTAAACCCTGATATGAAACGCGAAGTTCCGTAAAAAGGACGGCG
GATATGTAAATCTAGAGGAT

Downstream 100 bases:

>100_bases
CCACGCCAACCGCCTCTTTGCAGGCGGTTTTTTTTCGTCTGTCGCTCGTGCTGGCGGGGCGCCTGTTTTTACAGCAGTGT
GGTGGCGACTCTGTGAATTC

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MPLKGRLFIAVSLLASSVSCAFAVDLPATVAPPSIQAGSWVLMDYTTGQVLTAGNEHQQRNPASLTKLMTGYVVDRAIDS
HRITFDDIVTVGKDAWAKGNPVFDGSSLMFLKPGDRVSVRDLSRGLIVDSGNDACVALADYVAGGQPQFVALMNQYVEKL
HLRDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPDVYHMYSQKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSGA
GFNLIASAVDGQRRLIAVVMGADSPKGREQQAAKLLHWGQQNFDTVQVLQKGQKVGTERIWYGDKEQIKLGTDQDFWLAL
PKAEVSRIKAKYVLDKKDLEAPIAANQRVGEISLYDGDKVVGHYPLVTLESINKGGVFSRMSDYLHHEL

Sequences:

>Translated_389_residues
MPLKGRLFIAVSLLASSVSCAFAVDLPATVAPPSIQAGSWVLMDYTTGQVLTAGNEHQQRNPASLTKLMTGYVVDRAIDS
HRITFDDIVTVGKDAWAKGNPVFDGSSLMFLKPGDRVSVRDLSRGLIVDSGNDACVALADYVAGGQPQFVALMNQYVEKL
HLRDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPDVYHMYSQKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSGA
GFNLIASAVDGQRRLIAVVMGADSPKGREQQAAKLLHWGQQNFDTVQVLQKGQKVGTERIWYGDKEQIKLGTDQDFWLAL
PKAEVSRIKAKYVLDKKDLEAPIAANQRVGEISLYDGDKVVGHYPLVTLESINKGGVFSRMSDYLHHEL
>Mature_388_residues
PLKGRLFIAVSLLASSVSCAFAVDLPATVAPPSIQAGSWVLMDYTTGQVLTAGNEHQQRNPASLTKLMTGYVVDRAIDSH
RITFDDIVTVGKDAWAKGNPVFDGSSLMFLKPGDRVSVRDLSRGLIVDSGNDACVALADYVAGGQPQFVALMNQYVEKLH
LRDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPDVYHMYSQKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSGAG
FNLIASAVDGQRRLIAVVMGADSPKGREQQAAKLLHWGQQNFDTVQVLQKGQKVGTERIWYGDKEQIKLGTDQDFWLALP
KAEVSRIKAKYVLDKKDLEAPIAANQRVGEISLYDGDKVVGHYPLVTLESINKGGVFSRMSDYLHHEL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=387, Percent_Identity=80.6201550387597, Blast_Score=655, Evalue=0.0,
Organism=Escherichia coli, GI1786851, Length=389, Percent_Identity=49.3573264781491, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1787062, Length=370, Percent_Identity=48.3783783783784, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI87082054, Length=256, Percent_Identity=26.171875, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42613; Mature: 42482

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKGRLFIAVSLLASSVSCAFAVDLPATVAPPSIQAGSWVLMDYTTGQVLTAGNEHQQR
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCC
NPASLTKLMTGYVVDRAIDSHRITFDDIVTVGKDAWAKGNPVFDGSSLMFLKPGDRVSVR
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEHH
DLSRGLIVDSGNDACVALADYVAGGQPQFVALMNQYVEKLHLRDTHFETVHGLDAPGQHS
HCCCCEEEECCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCC
SAYDLAVLSRAIIHGEPDVYHMYSQKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSGA
CHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCC
GFNLIASAVDGQRRLIAVVMGADSPKGREQQAAKLLHWGQQNFDTVQVLQKGQKVGTERI
CHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEE
WYGDKEQIKLGTDQDFWLALPKAEVSRIKAKYVLDKKDLEAPIAANQRVGEISLYDGDKV
ECCCCCEEEECCCCCEEEECCHHHHHHHHHHEEECHHCCCCCCCCCCCCCEEEEECCCEE
VGHYPLVTLESINKGGVFSRMSDYLHHEL
EECCCEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PLKGRLFIAVSLLASSVSCAFAVDLPATVAPPSIQAGSWVLMDYTTGQVLTAGNEHQQR
CCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCC
NPASLTKLMTGYVVDRAIDSHRITFDDIVTVGKDAWAKGNPVFDGSSLMFLKPGDRVSVR
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEHH
DLSRGLIVDSGNDACVALADYVAGGQPQFVALMNQYVEKLHLRDTHFETVHGLDAPGQHS
HCCCCEEEECCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCC
SAYDLAVLSRAIIHGEPDVYHMYSQKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSGA
CHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCC
GFNLIASAVDGQRRLIAVVMGADSPKGREQQAAKLLHWGQQNFDTVQVLQKGQKVGTERI
CHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEE
WYGDKEQIKLGTDQDFWLALPKAEVSRIKAKYVLDKKDLEAPIAANQRVGEISLYDGDKV
ECCCCCEEEECCCCCEEEECCHHHHHHHHHHEEECHHCCCCCCCCCCCCCEEEEECCCEE
VGHYPLVTLESINKGGVFSRMSDYLHHEL
EECCCEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]