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The map label for this gene is gcvA [H]

Identifier: 238895572

GI number: 238895572

Start: 3476322

End: 3477236

Strand: Direct

Name: gcvA [H]

Synonym: KP1_3655

Alternate gene names: 238895572

Gene position: 3476322-3477236 (Clockwise)

Preceding gene: 238895568

Following gene: 238895575

Centisome position: 66.23

GC content: 64.7

Gene sequence:

>915_bases
ATGAGACCGCCCCGTCTGCCGCCGCTGGGGGCGCTACGCGCTTTTCATGCCGTCGCCCGCCACCGCAGTTTTAAACAGGC
CGCCGAGGCGCTCAGCGTCAGCGCGACGGCGGTCAGCCATCAGATTAAACTGCTGGAGTCGGTGCTGGAGTGCCGGGTCT
GCGAACGCAGCGCGCAGGGCGTCAGTCTGACCGCTGATGGCGAGATCCTTTACGCGGCCACCCAACGCGCCTTTAGCGCC
CTGGAACAGGCCGTCGTCCAGATCGCTCATGCCCGTCAGCCGCCATCCTTAACCGTCACCACCACCTCCAATTTTCTCAC
CCACTGGCTGGTGCCGCGGCTGGCGGATTTCACCGCCCGCTTTCCGGCTATCGACCTGCGGCTACACACCAGCGTCGAAC
GAGTGGATCTGCATCTGGGCACGGTAGACGCCGCGATTCGCTATCGTGAAAAGCCGGAGCCGGACCTGTACTGCACGCTG
CTGTATGAGGATCGCTTTATCGTGGTCGCCAGCCCGACTCTGGGCCTCAGCCGGCCTGAAGATCTGCAGCGGGTGACGCT
GTTTCACGTTGCCAACCGGCGCGTGCCAGCTGATTCGCCGAGCTGGGAGAACTGGCGTCGGCGCTATGGACCGCCGACGC
TGAATATTGATGCGGGGTTAACCTTTAGCGATGAGACCCATGCCCTGCAGGCGGCGGTGGCCGGTCAGGGAGTGGTGATC
GCCAGCGAACTGCTGGCGCGGGATCTGCTGCAGCGCGGCGTATTGTCCGCGCCGTTCAGCAACGCATTGCCTGGCGCCCG
CTACTACCTGGTCACTACCGAAGCGGTAGCGCAGCGGGCGGATATTATCGCTCTGCGGGAGTGGCTGCTCAGTCAGATGG
CGTCGGGCGACGGCGGCCATCCGACTGCGGGATAG

Upstream 100 bases:

>100_bases
TTTAATTCATGTCATTGTCTTTACATGCCTGTAGCCTATCGCCACTATATTGCCTTACACAAACGCATAGTTTTCAGCCA
TAGATGAGAAAAACTCAGCC

Downstream 100 bases:

>100_bases
CGGGCTTACCAGCCGCAGACGTTGGTCTCTTCATCGGACTCGTAGCCGCGAACGATATTGATCAGATCCTTCACCGCATC
AGCCGCGGTGAGTGGGTCGC

Product: putative LysR family regulatory protein

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRPPRLPPLGALRAFHAVARHRSFKQAAEALSVSATAVSHQIKLLESVLECRVCERSAQGVSLTADGEILYAATQRAFSA
LEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTARFPAIDLRLHTSVERVDLHLGTVDAAIRYREKPEPDLYCTL
LYEDRFIVVASPTLGLSRPEDLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGHPTAG

Sequences:

>Translated_304_residues
MRPPRLPPLGALRAFHAVARHRSFKQAAEALSVSATAVSHQIKLLESVLECRVCERSAQGVSLTADGEILYAATQRAFSA
LEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTARFPAIDLRLHTSVERVDLHLGTVDAAIRYREKPEPDLYCTL
LYEDRFIVVASPTLGLSRPEDLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGHPTAG
>Mature_304_residues
MRPPRLPPLGALRAFHAVARHRSFKQAAEALSVSATAVSHQIKLLESVLECRVCERSAQGVSLTADGEILYAATQRAFSA
LEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTARFPAIDLRLHTSVERVDLHLGTVDAAIRYREKPEPDLYCTL
LYEDRFIVVASPTLGLSRPEDLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGHPTAG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=37.3737373737374, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1786448, Length=297, Percent_Identity=34.006734006734, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=30.4054054054054, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081978, Length=244, Percent_Identity=28.2786885245902, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787128, Length=144, Percent_Identity=32.6388888888889, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=187, Percent_Identity=34.2245989304813, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=305, Percent_Identity=25.9016393442623, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693193, Length=275, Percent_Identity=26.5454545454545, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33339; Mature: 33339

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPRLPPLGALRAFHAVARHRSFKQAAEALSVSATAVSHQIKLLESVLECRVCERSAQG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSLTADGEILYAATQRAFSALEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTAR
EEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHC
FPAIDLRLHTSVERVDLHLGTVDAAIRYREKPEPDLYCTLLYEDRFIVVASPTLGLSRPE
CCEEEEEEECCCEEHEEECCHHHHHHHHCCCCCCCEEEEEEEECEEEEEECCCCCCCCCH
DLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEECCCCEECCCHHHHHHHHCCCCHHH
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGH
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTAG
CCCC
>Mature Secondary Structure
MRPPRLPPLGALRAFHAVARHRSFKQAAEALSVSATAVSHQIKLLESVLECRVCERSAQG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSLTADGEILYAATQRAFSALEQAVVQIAHARQPPSLTVTTTSNFLTHWLVPRLADFTAR
EEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHC
FPAIDLRLHTSVERVDLHLGTVDAAIRYREKPEPDLYCTLLYEDRFIVVASPTLGLSRPE
CCEEEEEEECCCEEHEEECCHHHHHHHHCCCCCCCEEEEEEEECEEEEEECCCCCCCCCH
DLQRVTLFHVANRRVPADSPSWENWRRRYGPPTLNIDAGLTFSDETHALQAAVAGQGVVI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEECCCCEECCCHHHHHHHHCCCCHHH
ASELLARDLLQRGVLSAPFSNALPGARYYLVTTEAVAQRADIIALREWLLSQMASGDGGH
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTAG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]