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The map label for this gene is nac [H]

Identifier: 238895498

GI number: 238895498

Start: 3383756

End: 3384754

Strand: Reverse

Name: nac [H]

Synonym: KP1_3570

Alternate gene names: 238895498

Gene position: 3384754-3383756 (Counterclockwise)

Preceding gene: 238895499

Following gene: 238895497

Centisome position: 64.49

GC content: 61.46

Gene sequence:

>999_bases
ATGCAAAGCTGGCAAGCAAATTGCAAAAGCCAGTGCATCATACTCCCAGCGTTGACGCTGGCGTTCCTTACAGGAGGCAA
AATGAACTTAAGACGACTGAAATATTTTGTGAAAATCGTCGATATCGGCAGCCTGACCCAGGCCGCTGAAGTCTTGCATA
TCGCCCAACCGGCCCTCAGCCAACAGGTCGCGACCCTGGAAGGTGAAATGGACCAGCAGTTGCTGATACGCACCAAGCGA
GGGGTCACGCCAACGGAAGCCGGTAAAATTCTCTACACCCATGCGCGCACTATCCTGCGCCAGTGCGAGCAGGCGCAGCT
GGCGGTCAATAACGTCGGCCAGACGCTGCGTGGTCAGGTGTCGATCGGCCTGGCGCCGGGCACCGCGGCCTCCGCCATTA
CCATGCCGTTGCTGCAAACGGTGCGCAATGAGTTGCCCGAGGTGATGGTCTATCTGCAGGAGAGCAGCGGCACAGCGCTG
AATGACAAGCTGCTGGCGGGCCAGCTGGATATGGCGGTGCTCTATGAGCGTTCGCCGGTGGCAGGCATCGTCAGCCAGCC
GCTGCTGAAAGAAGACCTTTACCTGGTCGGCACCCGCGACTGCCCGGGGCAGAGCGTGGATCTCACGGCGGTGGCGGAGA
TGAACCTGTTCCTGCCGCGCGATTACAGCGCAGTGCGGGCGCGGGTCACCGAGGCATTCACCCTGCGTCGTCTGTCGGCG
AAAATTATCGGCGAGATCGAATCCATCACCACCCTGACCGCGGCCATCGCCAGCGGCATGGGGGCGACGGTGTTACCGGA
ATCCGCTGCTCGTTCCCTGTGCGGGGCGGCGAACGGCTGGATGGCGCGGATCAGCACGCCTTCAATGAGCCTGTCGCTGT
CGCTGAACATGTCGGCTCGCGGCAGTCTGTCGCCGCAGGCGCAGGCGGTGAAAGAGATCCTGCTGTCTCTGGTGAGCCGC
CCATCGCTGGAAAACCGTGAACTGCAGCTGGTCAGTTAG

Upstream 100 bases:

>100_bases
CCTAATGGGGCATACCACAATCTGCTTACCGGGCAAATAAAAACCGAATTGCTATCACTATCCCTTATAACACTGCCTGC
GGCGGGCGACAGCCGAGGAA

Downstream 100 bases:

>100_bases
GGTATATTCTCTTAAGGAATAAGTTGCTGGTTTTTATTATTTGTTACCTGGGACAACAGACTTTAACAATAGCAGATACG
TTAAGTCCATTGGCCCGGAG

Product: nitrogen assimilation transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQSWQANCKSQCIILPALTLAFLTGGKMNLRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEMDQQLLIRTKR
GVTPTEAGKILYTHARTILRQCEQAQLAVNNVGQTLRGQVSIGLAPGTAASAITMPLLQTVRNELPEVMVYLQESSGTAL
NDKLLAGQLDMAVLYERSPVAGIVSQPLLKEDLYLVGTRDCPGQSVDLTAVAEMNLFLPRDYSAVRARVTEAFTLRRLSA
KIIGEIESITTLTAAIASGMGATVLPESAARSLCGAANGWMARISTPSMSLSLSLNMSARGSLSPQAQAVKEILLSLVSR
PSLENRELQLVS

Sequences:

>Translated_332_residues
MQSWQANCKSQCIILPALTLAFLTGGKMNLRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEMDQQLLIRTKR
GVTPTEAGKILYTHARTILRQCEQAQLAVNNVGQTLRGQVSIGLAPGTAASAITMPLLQTVRNELPEVMVYLQESSGTAL
NDKLLAGQLDMAVLYERSPVAGIVSQPLLKEDLYLVGTRDCPGQSVDLTAVAEMNLFLPRDYSAVRARVTEAFTLRRLSA
KIIGEIESITTLTAAIASGMGATVLPESAARSLCGAANGWMARISTPSMSLSLSLNMSARGSLSPQAQAVKEILLSLVSR
PSLENRELQLVS
>Mature_332_residues
MQSWQANCKSQCIILPALTLAFLTGGKMNLRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEMDQQLLIRTKR
GVTPTEAGKILYTHARTILRQCEQAQLAVNNVGQTLRGQVSIGLAPGTAASAITMPLLQTVRNELPEVMVYLQESSGTAL
NDKLLAGQLDMAVLYERSPVAGIVSQPLLKEDLYLVGTRDCPGQSVDLTAVAEMNLFLPRDYSAVRARVTEAFTLRRLSA
KIIGEIESITTLTAAIASGMGATVLPESAARSLCGAANGWMARISTPSMSLSLSLNMSARGSLSPQAQAVKEILLSLVSR
PSLENRELQLVS

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=305, Percent_Identity=79.672131147541, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1790399, Length=242, Percent_Identity=28.5123966942149, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI157672245, Length=243, Percent_Identity=28.3950617283951, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=25.8196721311475, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788887, Length=247, Percent_Identity=31.1740890688259, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=28.0632411067194, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787601, Length=180, Percent_Identity=29.4444444444444, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=28, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788748, Length=290, Percent_Identity=26.551724137931, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI2367136, Length=263, Percent_Identity=26.9961977186312, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787806, Length=254, Percent_Identity=25.1968503937008, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789204, Length=274, Percent_Identity=24.0875912408759, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789639, Length=144, Percent_Identity=31.9444444444444, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35660; Mature: 35660

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSWQANCKSQCIILPALTLAFLTGGKMNLRRLKYFVKIVDIGSLTQAAEVLHIAQPALS
CCCCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QQVATLEGEMDQQLLIRTKRGVTPTEAGKILYTHARTILRQCEQAQLAVNNVGQTLRGQV
HHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHCCEE
SIGLAPGTAASAITMPLLQTVRNELPEVMVYLQESSGTALNDKLLAGQLDMAVLYERSPV
EEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHCCCCEEEEECCCCC
AGIVSQPLLKEDLYLVGTRDCPGQSVDLTAVAEMNLFLPRDYSAVRARVTEAFTLRRLSA
HHHHHCCHHHCCEEEEECCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
KIIGEIESITTLTAAIASGMGATVLPESAARSLCGAANGWMARISTPSMSLSLSLNMSAR
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCC
GSLSPQAQAVKEILLSLVSRPSLENRELQLVS
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEC
>Mature Secondary Structure
MQSWQANCKSQCIILPALTLAFLTGGKMNLRRLKYFVKIVDIGSLTQAAEVLHIAQPALS
CCCCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QQVATLEGEMDQQLLIRTKRGVTPTEAGKILYTHARTILRQCEQAQLAVNNVGQTLRGQV
HHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHCCEE
SIGLAPGTAASAITMPLLQTVRNELPEVMVYLQESSGTALNDKLLAGQLDMAVLYERSPV
EEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHCCCCEEEEECCCCC
AGIVSQPLLKEDLYLVGTRDCPGQSVDLTAVAEMNLFLPRDYSAVRARVTEAFTLRRLSA
HHHHHCCHHHCCEEEEECCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
KIIGEIESITTLTAAIASGMGATVLPESAARSLCGAANGWMARISTPSMSLSLSLNMSAR
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCC
GSLSPQAQAVKEILLSLVSRPSLENRELQLVS
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]