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The map label for this gene is pykA [H]

Identifier: 238895431

GI number: 238895431

Start: 3317571

End: 3319037

Strand: Direct

Name: pykA [H]

Synonym: KP1_3499

Alternate gene names: 238895431

Gene position: 3317571-3319037 (Clockwise)

Preceding gene: 238895430

Following gene: 238895435

Centisome position: 63.21

GC content: 59.17

Gene sequence:

>1467_bases
TTGTATCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACCAAAATCGTTACTACCTTAGGCCCGGCAACTGA
CCGCGATAACAACCTGGAAAAGGTTATCGCCGCGGGCGCAAACGTAGTACGTATGAACTTCTCTCACGGCACCCCGGAAG
ATCATCAGCTCCGTGCCGACAAAGTGCGTGAGATTGCGGCAAAACTGGGCCGTCATGTGGCCATCCTCGGCGACCTCCAG
GGACCAAAAATTCGCGTATCGACCTTCAAAGAAGGCAAAATTTTCCTCAACGTCGGCGATAAATTCCTGCTCGACGCAAA
CCTTGGCAAAGGCGAAGGCGACAAAGAGAAAGTCGGGATCGACTATAAAGGCCTGCCGGCGGACGTCGTGCCGGGCGATA
TCTTATTGCTCGACGATGGTCGCGTGCAGCTGAAAGTCCTCGAAGTTCAGGGGATGAAAGTTTTCACTGAAGTGACCGTC
GGCGGCCCGTTGTCCAACAACAAAGGGATCAACAAGCTGGGCGGCGGCCTGTCTGCCGAAGCGCTGACCGACAAAGATAA
AGCTGATATCGTCACCGCCGCTAAAATCGGCGTCGACTACCTGGCCGTCTCTTTCCCGCGCTGCGGCGAAGACCTGAACT
ACGCCCGTCGCCTGGCGCGCGATGCCGGCTGCGATGCGAAAATCGTCGCCAAAGTTGAGCGTGCCGAAGCGGTTTGCGAT
CAGGATGCGATGGACGACGTGATCCTCGCCTCCGATGTGGTAATGGTTGCCCGTGGCGACCTCGGCGTCGAGATCGGCGA
TCCTGAGCTGGTGGGCATCCAGAAAGCGCTGATCCGTCGTGCCCGTCAGCTGAACCGTTCCGTCATCACCGCCACCCAGA
TGATGGAGTCGATGATCACCAACCCGATGCCGACTCGCGCGGAAGTGATGGACGTGGCCAACGCCGTGCTGGATGGTACC
GATGCGGTGATGCTCTCCGCGGAGACCGCTGCCGGTCAGTATCCTTCGGAAACCGTGGCCGCGATGGCGCGCGTGTGTCT
GGGCGCGGAAAAAATCCCTAGCCTCAACGTCTCCAAACACCGTCTGGACGTCCAGTTCGACAACGTGGAAGAAGCGATTG
CCATGTCGGCGATGTATGCCGCCAACCACCTGAAAGGCATCACCGCCATCATCACCATGACCGAATCGGGCCGTACCGCG
CTGATGACTTCGCGCATTAGCTCCGGTCTGCCGATTTTCGCCCTCTCCCGCCACGAGCGCACCCTGAACCTGACCGCCCT
CTACCGCGGGGTGACGCCGGTGTTCTTCGACAGCCAGAATGACGGGGTGGCCGCCGCGCACGACGCCGTCAATCTGCTGC
GGGACAAAGGCTATCTGGTCTCCGGCGACCTCGTGGTCGTTACCCAGGGTGACGTGATGAGCACCATCGGCAGCACCAAC
ACCACCCGTATCCTCACCGTCGAGTAA

Upstream 100 bases:

>100_bases
AGTTGATAACAAGCAGTACCCCATTACACCTTCGATAACGATCCGGCTGTGCGACGTTGCAGGCTGGCGCTACCGAATCC
ATGTTCACGCAACACCAAAG

Downstream 100 bases:

>100_bases
GCACGGTAGAGATAAAAAAGCCTCCTGACGGAGGCTTTTTTTATTTCTTTTTCGTGAACAGCTCTTTGCGTTTATAGGGC
TCGATTTCACCCGGCTTGCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MYQSTEYYMSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGTPEDHQLRADKVREIAAKLGRHVAILGDLQ
GPKIRVSTFKEGKIFLNVGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTV
GGPLSNNKGINKLGGGLSAEALTDKDKADIVTAAKIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCD
QDAMDDVILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRSVITATQMMESMITNPMPTRAEVMDVANAVLDGT
DAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSLNVSKHRLDVQFDNVEEAIAMSAMYAANHLKGITAIITMTESGRTA
LMTSRISSGLPIFALSRHERTLNLTALYRGVTPVFFDSQNDGVAAAHDAVNLLRDKGYLVSGDLVVVTQGDVMSTIGSTN
TTRILTVE

Sequences:

>Translated_488_residues
MYQSTEYYMSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGTPEDHQLRADKVREIAAKLGRHVAILGDLQ
GPKIRVSTFKEGKIFLNVGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTV
GGPLSNNKGINKLGGGLSAEALTDKDKADIVTAAKIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCD
QDAMDDVILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRSVITATQMMESMITNPMPTRAEVMDVANAVLDGT
DAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSLNVSKHRLDVQFDNVEEAIAMSAMYAANHLKGITAIITMTESGRTA
LMTSRISSGLPIFALSRHERTLNLTALYRGVTPVFFDSQNDGVAAAHDAVNLLRDKGYLVSGDLVVVTQGDVMSTIGSTN
TTRILTVE
>Mature_488_residues
MYQSTEYYMSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGTPEDHQLRADKVREIAAKLGRHVAILGDLQ
GPKIRVSTFKEGKIFLNVGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTV
GGPLSNNKGINKLGGGLSAEALTDKDKADIVTAAKIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCD
QDAMDDVILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRSVITATQMMESMITNPMPTRAEVMDVANAVLDGT
DAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSLNVSKHRLDVQFDNVEEAIAMSAMYAANHLKGITAIITMTESGRTA
LMTSRISSGLPIFALSRHERTLNLTALYRGVTPVFFDSQNDGVAAAHDAVNLLRDKGYLVSGDLVVVTQGDVMSTIGSTN
TTRILTVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=502, Percent_Identity=36.4541832669323, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI33286420, Length=502, Percent_Identity=36.4541832669323, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=36.7676767676768, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=34.412955465587, Blast_Score=232, Evalue=7e-61,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=34.412955465587, Blast_Score=232, Evalue=7e-61,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=34.8275862068965, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=34.8275862068965, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.0543933054393, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.0543933054393, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=32.5581395348837, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=95.625, Blast_Score=937, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=489, Percent_Identity=36.40081799591, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=490, Percent_Identity=35.9183673469388, Blast_Score=262, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=37.0445344129555, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=37.0445344129555, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=37.0445344129555, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=37.0445344129555, Blast_Score=238, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6324923, Length=500, Percent_Identity=35, Blast_Score=252, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6319279, Length=491, Percent_Identity=36.0488798370672, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.0445344129555, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.0445344129555, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24648966, Length=422, Percent_Identity=35.0710900473934, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=31.5682281059063, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24646914, Length=273, Percent_Identity=35.1648351648352, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52489; Mature: 52489

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQSTEYYMSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGTPEDHQLRAD
CCCCHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHH
KVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKIFLNVGDKFLLDANLGKGEGDKEKVGI
HHHHHHHHHCCEEEEEECCCCCEEEEEEEECCEEEEEECCEEEEECCCCCCCCCCCEECC
DYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAE
CCCCCCCCCCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCCH
ALTDKDKADIVTAAKIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCD
HCCCCCHHCEEEHHHHCHHEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
QDAMDDVILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRSVITATQMMESMIT
CCHHCCCHHHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSLNVSKH
CCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCE
RLDVQFDNVEEAIAMSAMYAANHLKGITAIITMTESGRTALMTSRISSGLPIFALSRHER
EEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEHHHHCCCCCEEEEECCCC
TLNLTALYRGVTPVFFDSQNDGVAAAHDAVNLLRDKGYLVSGDLVVVTQGDVMSTIGSTN
EEEEEEHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEECCEEEEECCCHHHHCCCCC
TTRILTVE
CEEEEEEC
>Mature Secondary Structure
MYQSTEYYMSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGTPEDHQLRAD
CCCCHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHH
KVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKIFLNVGDKFLLDANLGKGEGDKEKVGI
HHHHHHHHHCCEEEEEECCCCCEEEEEEEECCEEEEEECCEEEEECCCCCCCCCCCEECC
DYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAE
CCCCCCCCCCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCCH
ALTDKDKADIVTAAKIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCD
HCCCCCHHCEEEHHHHCHHEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
QDAMDDVILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRSVITATQMMESMIT
CCHHCCCHHHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSLNVSKH
CCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCE
RLDVQFDNVEEAIAMSAMYAANHLKGITAIITMTESGRTALMTSRISSGLPIFALSRHER
EEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEHHHHCCCCCEEEEECCCC
TLNLTALYRGVTPVFFDSQNDGVAAAHDAVNLLRDKGYLVSGDLVVVTQGDVMSTIGSTN
EEEEEEHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEECCEEEEECCCHHHHCCCCC
TTRILTVE
CEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]