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The map label for this gene is pckA [H]

Identifier: 238895339

GI number: 238895339

Start: 3222777

End: 3224420

Strand: Reverse

Name: pckA [H]

Synonym: KP1_3400

Alternate gene names: 238895339

Gene position: 3224420-3222777 (Counterclockwise)

Preceding gene: 238895341

Following gene: 238895337

Centisome position: 61.43

GC content: 61.25

Gene sequence:

>1644_bases
ATGTCACAACAAATTGAATCAGTAAAAATGGCTCTGCAGGAGCTGGGGATTAATGCAAGCGGCGAATTCTATTACAACCC
TGATTACGATTTGCTTATTGCACATGAAACCTCTCCTGAGCTGACCGGCGCGGCCCGCGGCGTGATGACCGACTCTGGCG
CTGTGGCTGTTGATACCGGGATCTTCACCGGCCGTTCGCCGCGCGATAAATACATCGTCCGCGACGAACAGACTCGCGAC
ACCGTCTGGTGGGCAGACTCCGGCCTCGGCCGCAACGACAACAAACCGTTATCGCCAGACGTGTGGCGCAGCCTGAAAAG
CCTGGTCGCGGGCCAGCTGAGCGGCAAAAAACTGTACGTCATCGACGCCTGGTGCGGCGCCAGCCCGGACACCCGTCTTG
GCGTACGCTTCGTCACCGAGGTGGCGTGGCAGGCCCACTTTGTCAAAAACATGTTCATCGTGCCAAGCGCCGACGAGCTG
GCCAGCTTTACGCCAGACTTCGTGGTGCTCAACGGCGCGGGCTGCACCAATGCCAACTGGCAGGCGCAGGGGATGAACTC
CGAGAACTTTGTCGCCTTTAATCTCAGCGAGCGTATTCAGCTCATCGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAG
GGCTGTTCTCGGTCATGAACTACCTGCTGCCGCAGAAAGGCATCGCGTCGATGCACTGCTCGGCCAACCGTGGCGAAGCA
GGGGACGTGGCGCTGTTCTTCGGCCTCTCCGGGACCGGGAAAACCACCCTGTCGACCGATCCGCATCGTCAGCTGATTGG
CGATGACGAGCACGGTTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCTAAGACCATCAATCTCGATC
CGCAGGCGGAGCCGGAGATTTATGGCGCCATCCGCCGCAATGCGCTGCTGGAAAACGTGGTGGTGCGCGCTGACGGCAGC
GTGGATTATGCCGACGGCAGCAAAACCGAAAACACCCGCGTCTCCTATCCGCTGTCGCATATCGACAATATCGTCAAGCC
GGTGTCCCGCGCGGGCCATCCGTCGAAGGTGATTTTCCTTGCCGCCGATGCGTTTGGCGTTCTGCCGCCGGTCTCCCGTC
TGACCACCGAGCAGATGCAGTACCATTTCCTGTCGGGTTTCACCTCGAAGCTGGCGGGCACCGAGCGCGGCATTACCCAG
CCGACGCCGACCTTCTCCGCCTGCTATGGCGCGGCGTTCCTGCTGCTTCACCCGACCCAGTACGCCAGCGTCCTGGCGGC
GAAGATGGCGGAATCCGGTGCCGAAGCCTGGCTGGTCAACACCGGCTGGAACGGCGAGGGCAAACGCCTGTCGCTGCGCG
ATACCCGCAGCATCATTAGCGCGATCCTCAACGGCACTACCGGCCCGCTGCGCGAGGAGACCATCCCGGTGTTCGGTCTT
GCGATCCCGCAGAGCATTCCAGGGGTAGACAGCGCGGTGCTGGATCCGCGTAATGGCTGGAGTTCAGCGGATAAATGGCA
GGAGAAGGCCGAGAGCCTGGCTCAGCTGTTTATGGATAACTTCAAGCAGTACAGCGATACCGAAGCGGGCGCGCGTCTGG
CGCTGGCCGGTCCGCAGCTGCAGAAGAGCGCGGTCGAAGCCTGA

Upstream 100 bases:

>100_bases
ATTTCATTGTCCAGGACTTATCTTGTTAAATAGCCGGCGACGATGACGAAAGCAATGAATAACCCAGGCCTCTTACCTTA
AATAGTTTGTCAGGAGTTCC

Downstream 100 bases:

>100_bases
TCCGCGGCCAGCAGGTCGACGCCTGGCGCCGACCTGCTGGCGAGCCGCTTTACTCCGCAAAGGCCCGCAGCGCATCGCCA
GTGAGGCGATAACGCACCCA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MSQQIESVKMALQELGINASGEFYYNPDYDLLIAHETSPELTGAARGVMTDSGAVAVDTGIFTGRSPRDKYIVRDEQTRD
TVWWADSGLGRNDNKPLSPDVWRSLKSLVAGQLSGKKLYVIDAWCGASPDTRLGVRFVTEVAWQAHFVKNMFIVPSADEL
ASFTPDFVVLNGAGCTNANWQAQGMNSENFVAFNLSERIQLIGGTWYGGEMKKGLFSVMNYLLPQKGIASMHCSANRGEA
GDVALFFGLSGTGKTTLSTDPHRQLIGDDEHGWDDDGVFNFEGGCYAKTINLDPQAEPEIYGAIRRNALLENVVVRADGS
VDYADGSKTENTRVSYPLSHIDNIVKPVSRAGHPSKVIFLAADAFGVLPPVSRLTTEQMQYHFLSGFTSKLAGTERGITQ
PTPTFSACYGAAFLLLHPTQYASVLAAKMAESGAEAWLVNTGWNGEGKRLSLRDTRSIISAILNGTTGPLREETIPVFGL
AIPQSIPGVDSAVLDPRNGWSSADKWQEKAESLAQLFMDNFKQYSDTEAGARLALAGPQLQKSAVEA

Sequences:

>Translated_547_residues
MSQQIESVKMALQELGINASGEFYYNPDYDLLIAHETSPELTGAARGVMTDSGAVAVDTGIFTGRSPRDKYIVRDEQTRD
TVWWADSGLGRNDNKPLSPDVWRSLKSLVAGQLSGKKLYVIDAWCGASPDTRLGVRFVTEVAWQAHFVKNMFIVPSADEL
ASFTPDFVVLNGAGCTNANWQAQGMNSENFVAFNLSERIQLIGGTWYGGEMKKGLFSVMNYLLPQKGIASMHCSANRGEA
GDVALFFGLSGTGKTTLSTDPHRQLIGDDEHGWDDDGVFNFEGGCYAKTINLDPQAEPEIYGAIRRNALLENVVVRADGS
VDYADGSKTENTRVSYPLSHIDNIVKPVSRAGHPSKVIFLAADAFGVLPPVSRLTTEQMQYHFLSGFTSKLAGTERGITQ
PTPTFSACYGAAFLLLHPTQYASVLAAKMAESGAEAWLVNTGWNGEGKRLSLRDTRSIISAILNGTTGPLREETIPVFGL
AIPQSIPGVDSAVLDPRNGWSSADKWQEKAESLAQLFMDNFKQYSDTEAGARLALAGPQLQKSAVEA
>Mature_546_residues
SQQIESVKMALQELGINASGEFYYNPDYDLLIAHETSPELTGAARGVMTDSGAVAVDTGIFTGRSPRDKYIVRDEQTRDT
VWWADSGLGRNDNKPLSPDVWRSLKSLVAGQLSGKKLYVIDAWCGASPDTRLGVRFVTEVAWQAHFVKNMFIVPSADELA
SFTPDFVVLNGAGCTNANWQAQGMNSENFVAFNLSERIQLIGGTWYGGEMKKGLFSVMNYLLPQKGIASMHCSANRGEAG
DVALFFGLSGTGKTTLSTDPHRQLIGDDEHGWDDDGVFNFEGGCYAKTINLDPQAEPEIYGAIRRNALLENVVVRADGSV
DYADGSKTENTRVSYPLSHIDNIVKPVSRAGHPSKVIFLAADAFGVLPPVSRLTTEQMQYHFLSGFTSKLAGTERGITQP
TPTFSACYGAAFLLLHPTQYASVLAAKMAESGAEAWLVNTGWNGEGKRLSLRDTRSIISAILNGTTGPLREETIPVFGLA
IPQSIPGVDSAVLDPRNGWSSADKWQEKAESLAQLFMDNFKQYSDTEAGARLALAGPQLQKSAVEA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=529, Percent_Identity=73.5349716446125, Blast_Score=841, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=507, Percent_Identity=47.3372781065089, Blast_Score=462, Evalue=1e-131,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59301; Mature: 59170

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQIESVKMALQELGINASGEFYYNPDYDLLIAHETSPELTGAARGVMTDSGAVAVDTG
CCHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCCCCCHHHCCEEECCCCEEEECC
IFTGRSPRDKYIVRDEQTRDTVWWADSGLGRNDNKPLSPDVWRSLKSLVAGQLSGKKLYV
EECCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
IDAWCGASPDTRLGVRFVTEVAWQAHFVKNMFIVPSADELASFTPDFVVLNGAGCTNANW
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCEEEECCHHHHHHCCCCEEEEECCCCCCCCE
QAQGMNSENFVAFNLSERIQLIGGTWYGGEMKKGLFSVMNYLLPQKGIASMHCSANRGEA
EECCCCCCCEEEEECCCEEEEEECEEECCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCC
GDVALFFGLSGTGKTTLSTDPHRQLIGDDEHGWDDDGVFNFEGGCYAKTINLDPQAEPEI
CCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCHHH
YGAIRRNALLENVVVRADGSVDYADGSKTENTRVSYPLSHIDNIVKPVSRAGHPSKVIFL
HHHHHHHHHHHHEEEEECCCEEECCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEE
AADAFGVLPPVSRLTTEQMQYHFLSGFTSKLAGTERGITQPTPTFSACYGAAFLLLHPTQ
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHEEEEEECCHH
YASVLAAKMAESGAEAWLVNTGWNGEGKRLSLRDTRSIISAILNGTTGPLREETIPVFGL
HHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
AIPQSIPGVDSAVLDPRNGWSSADKWQEKAESLAQLFMDNFKQYSDTEAGARLALAGPQL
ECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHH
QKSAVEA
HHHHCCC
>Mature Secondary Structure 
SQQIESVKMALQELGINASGEFYYNPDYDLLIAHETSPELTGAARGVMTDSGAVAVDTG
CHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCCCCCHHHCCEEECCCCEEEECC
IFTGRSPRDKYIVRDEQTRDTVWWADSGLGRNDNKPLSPDVWRSLKSLVAGQLSGKKLYV
EECCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
IDAWCGASPDTRLGVRFVTEVAWQAHFVKNMFIVPSADELASFTPDFVVLNGAGCTNANW
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCEEEECCHHHHHHCCCCEEEEECCCCCCCCE
QAQGMNSENFVAFNLSERIQLIGGTWYGGEMKKGLFSVMNYLLPQKGIASMHCSANRGEA
EECCCCCCCEEEEECCCEEEEEECEEECCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCC
GDVALFFGLSGTGKTTLSTDPHRQLIGDDEHGWDDDGVFNFEGGCYAKTINLDPQAEPEI
CCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCHHH
YGAIRRNALLENVVVRADGSVDYADGSKTENTRVSYPLSHIDNIVKPVSRAGHPSKVIFL
HHHHHHHHHHHHEEEEECCCEEECCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEE
AADAFGVLPPVSRLTTEQMQYHFLSGFTSKLAGTERGITQPTPTFSACYGAAFLLLHPTQ
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHEEEEEECCHH
YASVLAAKMAESGAEAWLVNTGWNGEGKRLSLRDTRSIISAILNGTTGPLREETIPVFGL
HHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
AIPQSIPGVDSAVLDPRNGWSSADKWQEKAESLAQLFMDNFKQYSDTEAGARLALAGPQL
ECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHH
QKSAVEA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA