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The map label for this gene is citH [H]

Identifier: 238895336

GI number: 238895336

Start: 3219627

End: 3221018

Strand: Reverse

Name: citH [H]

Synonym: KP1_3396

Alternate gene names: 238895336

Gene position: 3221018-3219627 (Counterclockwise)

Preceding gene: 238895337

Following gene: 238895335

Centisome position: 61.37

GC content: 60.13

Gene sequence:

>1392_bases
GTGGATGCGTTTATTACGCTTATTTCGTTCCCGGTTCTGTTTCAGTCTTCAGCGACAGGCGCGCGACAACGCGTGGATAA
CAAACTGGAGACCTGCATGCATTCAACAACCTCACAGATGAGTACCCGTGACCGGATCGGCGCCATTCTGCGCGTCACGT
CCGGTAACTTTCTCGAACAGTTCGACTTTTTCCTGTTCGGGTTTTATGCCACCTACATCGCCCATACCTTTTTTCCGGCG
AGCAGTGAATTCGCCTCGCTGATGATGACCTTCGCAGTCTTTGGCGCCGGCTTTTTGATGCGCCCCATCGGCGCTATCGT
ACTTGGCGCCTATATCGACAAGGTGGGGCGGCGCAAGGGGCTGATCGTCACCCTGTCGATCATGGCCACCGGCACCTTCC
TGATCGTGCTGATCCCCTCTTATCAGACCATTGGCCTGTGGGCGCCGCTGCTGGTGCTGATCGGCCGTCTGCTGCAGGGC
TTTTCCGCCGGCGCCGAGCTGGGCGGGGTGTCGGTCTATCTGGCCGAGATCGCCACCCCGGGCCGCAAAGGCTTTTACAC
CAGCTGGCAGTCGGGCAGTCAGCAGGTTGCCATCATGGTGGCAGCCGCGATGGGCTTTGCCCTCAACGCGGTACTGGAGC
CGAGCGCTATCAGCGACTGGGGCTGGCGTATTCCGTTCCTTTTCGGCTGCCTGATTGTTCCGTTCATTTTTATCCTGCGC
CGTAAGCTGGAGGAGACCCAGGAGTTTACTGCCCGCCGCCATCATCTGGCGATGCGCCAGGTATTCGCCACCCTGCTGGC
GAACTGGCAGGTGGTTATCGCCGGGATGATGATGGTGGCGATGACCACCACCGCGTTCTACCTGATCACCGTCTATGCCC
CGACCTTTGGTAAAAAGGTGCTGATGCTCAGTGCCTCTGACAGCCTGCTGGTCACGCTGCTGGTGGCGATTTCCAACTTC
TTCTGGCTGCCGGTGGGCGGGGCGCTGTCCGACCGCTTTGGCCGCCGATCGGTGCTGATCGCCATGACCCTGCTGGCGCT
GGCTACCGCCTGGCCTGCGCTGACCATGCTGGCGAACGCCCCGAGCTTTTTGATGATGCTGAGCGTGCTGCTGTGGCTGT
CGTTTATCTACGGCATGTACAACGGGGCGATGATCCCGGCGCTGACCGAAATTATGCCCGCCGAAGTGCGCGTGGCAGGT
TTCTCGCTGGCCTACAGCCTGGCGACCGCGGTGTTTGGCGGCTTCACGCCGGTGATTTCCACAGCGCTGATTGAGTATAC
CGGTGATAAAGCGTCTCCCGGCTACTGGATGAGCTTTGCCGCCATCTGCGGTCTGCTGGCCACTTGCTACCTCTATCGCC
GTAGCGCCGTTGCGCTGCAGACGGCACGTTAA

Upstream 100 bases:

>100_bases
TCGCCGTCACCCGCGGCGTGGTTAGGACGGTGTGTGTTAAATTATTGTATGTAAAGGTAATGTTAATTAGTTAAATGTTA
TTCATGCGTAAAATGCATTA

Downstream 100 bases:

>100_bases
TCAGGGAGAAGATGATGCGTAAAACGACAGGAACCCTGCTCGCCACGCTGCTGCTGGCCGCCACCGGCGGCAGCGCGTTG
TCCGCTGAGGTCACCGTGAT

Product: putative permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MDAFITLISFPVLFQSSATGARQRVDNKLETCMHSTTSQMSTRDRIGAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPA
SSEFASLMMTFAVFGAGFLMRPIGAIVLGAYIDKVGRRKGLIVTLSIMATGTFLIVLIPSYQTIGLWAPLLVLIGRLLQG
FSAGAELGGVSVYLAEIATPGRKGFYTSWQSGSQQVAIMVAAAMGFALNAVLEPSAISDWGWRIPFLFGCLIVPFIFILR
RKLEETQEFTARRHHLAMRQVFATLLANWQVVIAGMMMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNF
FWLPVGGALSDRFGRRSVLIAMTLLALATAWPALTMLANAPSFLMMLSVLLWLSFIYGMYNGAMIPALTEIMPAEVRVAG
FSLAYSLATAVFGGFTPVISTALIEYTGDKASPGYWMSFAAICGLLATCYLYRRSAVALQTAR

Sequences:

>Translated_463_residues
MDAFITLISFPVLFQSSATGARQRVDNKLETCMHSTTSQMSTRDRIGAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPA
SSEFASLMMTFAVFGAGFLMRPIGAIVLGAYIDKVGRRKGLIVTLSIMATGTFLIVLIPSYQTIGLWAPLLVLIGRLLQG
FSAGAELGGVSVYLAEIATPGRKGFYTSWQSGSQQVAIMVAAAMGFALNAVLEPSAISDWGWRIPFLFGCLIVPFIFILR
RKLEETQEFTARRHHLAMRQVFATLLANWQVVIAGMMMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNF
FWLPVGGALSDRFGRRSVLIAMTLLALATAWPALTMLANAPSFLMMLSVLLWLSFIYGMYNGAMIPALTEIMPAEVRVAG
FSLAYSLATAVFGGFTPVISTALIEYTGDKASPGYWMSFAAICGLLATCYLYRRSAVALQTAR
>Mature_463_residues
MDAFITLISFPVLFQSSATGARQRVDNKLETCMHSTTSQMSTRDRIGAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPA
SSEFASLMMTFAVFGAGFLMRPIGAIVLGAYIDKVGRRKGLIVTLSIMATGTFLIVLIPSYQTIGLWAPLLVLIGRLLQG
FSAGAELGGVSVYLAEIATPGRKGFYTSWQSGSQQVAIMVAAAMGFALNAVLEPSAISDWGWRIPFLFGCLIVPFIFILR
RKLEETQEFTARRHHLAMRQVFATLLANWQVVIAGMMMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNF
FWLPVGGALSDRFGRRSVLIAMTLLALATAWPALTMLANAPSFLMMLSVLLWLSFIYGMYNGAMIPALTEIMPAEVRVAG
FSLAYSLATAVFGGFTPVISTALIEYTGDKASPGYWMSFAAICGLLATCYLYRRSAVALQTAR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=435, Percent_Identity=33.7931034482759, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=30.607476635514, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788292, Length=415, Percent_Identity=30.6024096385542, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1789941, Length=390, Percent_Identity=29.7435897435897, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87082231, Length=237, Percent_Identity=25.7383966244726, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50558; Mature: 50558

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAFITLISFPVLFQSSATGARQRVDNKLETCMHSTTSQMSTRDRIGAILRVTSGNFLEQ
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHH
FDFFLFGFYATYIAHTFFPASSEFASLMMTFAVFGAGFLMRPIGAIVLGAYIDKVGRRKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LIVTLSIMATGTFLIVLIPSYQTIGLWAPLLVLIGRLLQGFSAGAELGGVSVYLAEIATP
EEEEEEHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHHCCC
GRKGFYTSWQSGSQQVAIMVAAAMGFALNAVLEPSAISDWGWRIPFLFGCLIVPFIFILR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RKLEETQEFTARRHHLAMRQVFATLLANWQVVIAGMMMVAMTTTAFYLITVYAPTFGKKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEE
LMLSASDSLLVTLLVAISNFFWLPVGGALSDRFGRRSVLIAMTLLALATAWPALTMLANA
EEEECCCHHHHHHHHHHHHHHEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PSFLMMLSVLLWLSFIYGMYNGAMIPALTEIMPAEVRVAGFSLAYSLATAVFGGFTPVIS
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
TALIEYTGDKASPGYWMSFAAICGLLATCYLYRRSAVALQTAR
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDAFITLISFPVLFQSSATGARQRVDNKLETCMHSTTSQMSTRDRIGAILRVTSGNFLEQ
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHH
FDFFLFGFYATYIAHTFFPASSEFASLMMTFAVFGAGFLMRPIGAIVLGAYIDKVGRRKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LIVTLSIMATGTFLIVLIPSYQTIGLWAPLLVLIGRLLQGFSAGAELGGVSVYLAEIATP
EEEEEEHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHHCCC
GRKGFYTSWQSGSQQVAIMVAAAMGFALNAVLEPSAISDWGWRIPFLFGCLIVPFIFILR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RKLEETQEFTARRHHLAMRQVFATLLANWQVVIAGMMMVAMTTTAFYLITVYAPTFGKKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEE
LMLSASDSLLVTLLVAISNFFWLPVGGALSDRFGRRSVLIAMTLLALATAWPALTMLANA
EEEECCCHHHHHHHHHHHHHHEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PSFLMMLSVLLWLSFIYGMYNGAMIPALTEIMPAEVRVAGFSLAYSLATAVFGGFTPVIS
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
TALIEYTGDKASPGYWMSFAAICGLLATCYLYRRSAVALQTAR
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]