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The map label for this gene is oppA [H]

Identifier: 238895256

GI number: 238895256

Start: 3132677

End: 3134311

Strand: Reverse

Name: oppA [H]

Synonym: KP1_3308

Alternate gene names: 238895256

Gene position: 3134311-3132677 (Counterclockwise)

Preceding gene: 238895257

Following gene: 238895255

Centisome position: 59.72

GC content: 53.33

Gene sequence:

>1635_bases
ATGACCATCATCACTAAAAAGCGTTTAATCGCCGCGGGAGTGCTATCCGCACTGATCGCCGGCAATATGGCAATGGCAGC
GGATGTCCCGGCTGGTGTGCAGCTGGCAGAGAAGCAAACCCTGGTACGTAATAGCGGCGCCGAGCCGCAATCGCTGGACC
CAAATAAAATCGAAGGGGTGCCTGAGGCCAATATTAGCCGCGACCTGTTCGAAGGTTTGCTGAATACTTCGCCGAAAGAT
GGCCACCCGATCCCTGGCGTGGCGGAGAGCTGGGATAATAAAGATTTTAAAGTCTGGACCTTCCATCTGCGTAAAGATGC
AAAATGGTCTAACGGCGAGCCGGTTACCGCCCAGGACTTCGTATACAGCTGGCAGCGTCTGGTGGATCCGAAAACAGCTT
CTCCTTATGCCAGCTATCCGCAATATGGTCATATCGTTAACGTCGACGAAATCATTGACGGCAAGAAAGCGCCGAGCGAG
CTGGGCGTAAAAGCCATTGACGACCACACGCTGGAAGTCACCCTCAGCGAACCGGTACCCTATTTTTATAAACTGCTGGT
TAACCCGGCGATGTCTCCCGTCTACAAACCGGCCATCGAAAAATTTGGCGAAAAGTGGACGCAGCCTGGCAATATCGTTA
CCAACGGCGCGTATACTCTGAAAGACTGGGTGGTCAACGAGCGGATCGTGATGGAGCGTAACCCGCACTATTGGGATAAT
GCTAAAACCGTCATTAACACCGTTACCTGGCTGCCGACATCTTCCGAAGTGACCTATGTTAACCGCTACCGCAGCGGTGA
ACTGGACATGACTTATAACCAGCTGCCGATCGAACTCTTCCAGAAGCTGAAAAAAGAGATCCCCAACGAGCTGCACGTTG
ACCCCTATCTGTGTACCTATTATTACGAAATCAACAACCAGAAGGCCCCGTTCACCGACGTGCGCGTGCGTACCGCGCTG
AAGCTGGGTCTGGATCGCGATATTATTGCTAATAAGGTGAAAGGACAGGGCGATCTGCCGGCCTACGGCTACACCCCACC
GTATACCGATGGCGCGAAGCTGAGCGAGCCGGAATGGTTTACCTGGTCGCAGGAAAAACGCAACGAAGAAGCGAAAAAAC
TGCTGGCCGAAGCCGGCTATAGCGCCGATAAACCGTTGACCTTCAACCTGCTGTATAACACCTCTGATTTGCACAAGAAG
CTGGCGATTGCCGCCGCCTCGCTGTGGCGTAAAAACCTGGGCATTGACGTGAAGCTGGTCAACCAGGAGTGGAAAACGTT
CCTCGATACCCGTCATCAGGGCACCTATGACGTGGCGCGTGCGGGCTGGTGCGCCGACTATAACGAGCCAACCTCTTTCC
TGAACACCATGCTCTCTGACAGTTCAATGAACACCGCGCACTATAAGAGCCCGGCGTTCGACAAGATTATGGCTGAATCC
GTAAAAGCATCCGATGAAGCGCAGCGCACGGCGGCCTATGCGAAAGCAGAGCAGCAGCTGGACAAAGACAGCGCGATCGT
ACCGGTCTATTACTACGTTAACGCCCGCCTGGTGAAACCGTGGGTCGGTGGCTATACCGGTAAAGACCCGATGGATAACG
TCTATACCAAAGACCTCTACGTTATCAAACATTAA

Upstream 100 bases:

>100_bases
AATCTGCCAATCGCAAACTCCTGCGTTGGACAGAACCCGAAAACGGGAAAAACAGGCTGGTTAAAAAACCAGTAATTATA
ATGACTGGAGTTACAACACA

Downstream 100 bases:

>100_bases
TGGCAATACGTGGAGCGGGTCTCGGCTCGCTCCATCGTGTCTTATTTGAGAGCAGTGCAAAGGCACACGCCAGAAGGTAC
GGGCAATGTTAAAATTTATC

Product: oligopeptide ABC transport system periplasmic binding component

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTIITKKRLIAAGVLSALIAGNMAMAADVPAGVQLAEKQTLVRNSGAEPQSLDPNKIEGVPEANISRDLFEGLLNTSPKD
GHPIPGVAESWDNKDFKVWTFHLRKDAKWSNGEPVTAQDFVYSWQRLVDPKTASPYASYPQYGHIVNVDEIIDGKKAPSE
LGVKAIDDHTLEVTLSEPVPYFYKLLVNPAMSPVYKPAIEKFGEKWTQPGNIVTNGAYTLKDWVVNERIVMERNPHYWDN
AKTVINTVTWLPTSSEVTYVNRYRSGELDMTYNQLPIELFQKLKKEIPNELHVDPYLCTYYYEINNQKAPFTDVRVRTAL
KLGLDRDIIANKVKGQGDLPAYGYTPPYTDGAKLSEPEWFTWSQEKRNEEAKKLLAEAGYSADKPLTFNLLYNTSDLHKK
LAIAAASLWRKNLGIDVKLVNQEWKTFLDTRHQGTYDVARAGWCADYNEPTSFLNTMLSDSSMNTAHYKSPAFDKIMAES
VKASDEAQRTAAYAKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYTGKDPMDNVYTKDLYVIKH

Sequences:

>Translated_544_residues
MTIITKKRLIAAGVLSALIAGNMAMAADVPAGVQLAEKQTLVRNSGAEPQSLDPNKIEGVPEANISRDLFEGLLNTSPKD
GHPIPGVAESWDNKDFKVWTFHLRKDAKWSNGEPVTAQDFVYSWQRLVDPKTASPYASYPQYGHIVNVDEIIDGKKAPSE
LGVKAIDDHTLEVTLSEPVPYFYKLLVNPAMSPVYKPAIEKFGEKWTQPGNIVTNGAYTLKDWVVNERIVMERNPHYWDN
AKTVINTVTWLPTSSEVTYVNRYRSGELDMTYNQLPIELFQKLKKEIPNELHVDPYLCTYYYEINNQKAPFTDVRVRTAL
KLGLDRDIIANKVKGQGDLPAYGYTPPYTDGAKLSEPEWFTWSQEKRNEEAKKLLAEAGYSADKPLTFNLLYNTSDLHKK
LAIAAASLWRKNLGIDVKLVNQEWKTFLDTRHQGTYDVARAGWCADYNEPTSFLNTMLSDSSMNTAHYKSPAFDKIMAES
VKASDEAQRTAAYAKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYTGKDPMDNVYTKDLYVIKH
>Mature_543_residues
TIITKKRLIAAGVLSALIAGNMAMAADVPAGVQLAEKQTLVRNSGAEPQSLDPNKIEGVPEANISRDLFEGLLNTSPKDG
HPIPGVAESWDNKDFKVWTFHLRKDAKWSNGEPVTAQDFVYSWQRLVDPKTASPYASYPQYGHIVNVDEIIDGKKAPSEL
GVKAIDDHTLEVTLSEPVPYFYKLLVNPAMSPVYKPAIEKFGEKWTQPGNIVTNGAYTLKDWVVNERIVMERNPHYWDNA
KTVINTVTWLPTSSEVTYVNRYRSGELDMTYNQLPIELFQKLKKEIPNELHVDPYLCTYYYEINNQKAPFTDVRVRTALK
LGLDRDIIANKVKGQGDLPAYGYTPPYTDGAKLSEPEWFTWSQEKRNEEAKKLLAEAGYSADKPLTFNLLYNTSDLHKKL
AIAAASLWRKNLGIDVKLVNQEWKTFLDTRHQGTYDVARAGWCADYNEPTSFLNTMLSDSSMNTAHYKSPAFDKIMAESV
KASDEAQRTAAYAKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYTGKDPMDNVYTKDLYVIKH

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=544, Percent_Identity=84.0073529411765, Blast_Score=964, Evalue=0.0,
Organism=Escherichia coli, GI87081878, Length=539, Percent_Identity=48.2374768089054, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1789397, Length=531, Percent_Identity=43.6911487758945, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1787762, Length=503, Percent_Identity=26.0437375745527, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789966, Length=538, Percent_Identity=24.907063197026, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789887, Length=469, Percent_Identity=25.1599147121535, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787052, Length=516, Percent_Identity=23.8372093023256, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787551, Length=526, Percent_Identity=23.7642585551331, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61389; Mature: 61258

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIITKKRLIAAGVLSALIAGNMAMAADVPAGVQLAEKQTLVRNSGAEPQSLDPNKIEGV
CCEECHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCCC
PEANISRDLFEGLLNTSPKDGHPIPGVAESWDNKDFKVWTFHLRKDAKWSNGEPVTAQDF
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCCHHHH
VYSWQRLVDPKTASPYASYPQYGHIVNVDEIIDGKKAPSELGVKAIDDHTLEVTLSEPVP
HHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHCCCCCHHHCCCEEECCCEEEEEECCCCH
YFYKLLVNPAMSPVYKPAIEKFGEKWTQPGNIVTNGAYTLKDWVVNERIVMERNPHYWDN
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECCCEEEHHHHCCCEEEEECCCCCCCC
AKTVINTVTWLPTSSEVTYVNRYRSGELDMTYNQLPIELFQKLKKEIPNELHVDPYLCTY
HHHHHHEEEECCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEE
YYEINNQKAPFTDVRVRTALKLGLDRDIIANKVKGQGDLPAYGYTPPYTDGAKLSEPEWF
EEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TWSQEKRNEEAKKLLAEAGYSADKPLTFNLLYNTSDLHKKLAIAAASLWRKNLGIDVKLV
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEE
NQEWKTFLDTRHQGTYDVARAGWCADYNEPTSFLNTMLSDSSMNTAHYKSPAFDKIMAES
CHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
VKASDEAQRTAAYAKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYTGKDPMDNVYTKDLY
HHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEECCCCCCCCCCCCCCCCCCCCEE
VIKH
EEEC
>Mature Secondary Structure 
TIITKKRLIAAGVLSALIAGNMAMAADVPAGVQLAEKQTLVRNSGAEPQSLDPNKIEGV
CEECHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCCC
PEANISRDLFEGLLNTSPKDGHPIPGVAESWDNKDFKVWTFHLRKDAKWSNGEPVTAQDF
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCCHHHH
VYSWQRLVDPKTASPYASYPQYGHIVNVDEIIDGKKAPSELGVKAIDDHTLEVTLSEPVP
HHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHCCCCCHHHCCCEEECCCEEEEEECCCCH
YFYKLLVNPAMSPVYKPAIEKFGEKWTQPGNIVTNGAYTLKDWVVNERIVMERNPHYWDN
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECCCEEEHHHHCCCEEEEECCCCCCCC
AKTVINTVTWLPTSSEVTYVNRYRSGELDMTYNQLPIELFQKLKKEIPNELHVDPYLCTY
HHHHHHEEEECCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEE
YYEINNQKAPFTDVRVRTALKLGLDRDIIANKVKGQGDLPAYGYTPPYTDGAKLSEPEWF
EEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TWSQEKRNEEAKKLLAEAGYSADKPLTFNLLYNTSDLHKKLAIAAASLWRKNLGIDVKLV
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEE
NQEWKTFLDTRHQGTYDVARAGWCADYNEPTSFLNTMLSDSSMNTAHYKSPAFDKIMAES
CHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
VKASDEAQRTAAYAKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYTGKDPMDNVYTKDLY
HHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEECCCCCCCCCCCCCCCCCCCCEE
VIKH
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187863; 9097039; 9278503; 2015910; 9298646; 9600841 [H]