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The map label for this gene is ywbI [H]

Identifier: 238895077

GI number: 238895077

Start: 2959142

End: 2960041

Strand: Reverse

Name: ywbI [H]

Synonym: KP1_3109

Alternate gene names: 238895077

Gene position: 2960041-2959142 (Counterclockwise)

Preceding gene: 238895087

Following gene: 238895076

Centisome position: 56.4

GC content: 58.67

Gene sequence:

>900_bases
ATGTTTGCCTTCTCCCGATTTCTGCTCTATTTCACCGAAGTGGCCCGTCAGGGATCGTTTCGTAAAGCGTCAGAGGCGCT
GCATGTGTCGGCGTCTTCCATCGACCGTCAGATTTTGCGCGTGGAACAGGAGCTGGCGATGCCGCTCTTCGAGCGCCATC
CCACCGGGCTGCGGCTGACCGCCGCCGGGGAGCTGCTCCTGCACGCGGCCAATAACTGGAAAAAGGACTTTACCCGGGTC
TGCGAGCAGCTGGATGACCTGCGCGGGCTGCGGCGCGGGCATGTGCGCATCGCCACCATCGATGCCATTAACCGGCATTT
CTTTTCGACGATGCTCAAAGAGGTGCATAAACATTATCCCAACATCTCTTTTACATTGACCACCATCAACAATATCCACA
TCCAGCAGGCGCTGATCTCCGGCGAGGCGGATTTCGGCATCATGCTCAATCCGCAGACCTCCCGTGAACTGCAGGTGAGG
GCGTTTGCCGAAATGAATATGGGTATCGTGGTGCCCACCGGCCATCCGCTGGCCAGCCGCAGCGCGGTGCGCTTTAGTCA
GTGCCTCGACTACCCCTTTATCCTGCCGTCCGCCCCATTGATGATCAGCGAGCCGGTGGAGGCGCTGGTGAACATCAGCG
GCAACGAGGTAAAGGAGGTGGCGGTATCGAATAACATTCACATGATCCGCACCCTGATCAAAGAGCAGATGGGCATCGGC
ATCCTGTGCCGGCTGGATATTCTCGATGAGATTGAGTCCGGCCAGCTGGCGTTTGTGCCGCTAACCGATCCGCAGCTTAA
GCCTTTCACCCTGGCGCTGTGCGTCTCGCCCGCCCGCCAGCTTTCCCTTGCCGCCTCGATGATGTTGAACCAGCTGGAGA
TGCTCTTCAGCCAGCTGTGA

Upstream 100 bases:

>100_bases
GATCGTTTCTCCGCTATGAATGCTACGGTATTGTGATAAAACTGTTGCGTCCAGAGCTTTGATTCGCACAACCCGTTGCA
AAAAAGAGAGCACCCAAACC

Downstream 100 bases:

>100_bases
AATGTCGGGCAGGCACTAAAATCATTCATTAGCGCACCATTATGGTGCGTTTCTTGTGGGCATGAATCAAAGGTTGCATG
CCCAAGGGCGGCGGCGCAGG

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MFAFSRFLLYFTEVARQGSFRKASEALHVSASSIDRQILRVEQELAMPLFERHPTGLRLTAAGELLLHAANNWKKDFTRV
CEQLDDLRGLRRGHVRIATIDAINRHFFSTMLKEVHKHYPNISFTLTTINNIHIQQALISGEADFGIMLNPQTSRELQVR
AFAEMNMGIVVPTGHPLASRSAVRFSQCLDYPFILPSAPLMISEPVEALVNISGNEVKEVAVSNNIHMIRTLIKEQMGIG
ILCRLDILDEIESGQLAFVPLTDPQLKPFTLALCVSPARQLSLAASMMLNQLEMLFSQL

Sequences:

>Translated_299_residues
MFAFSRFLLYFTEVARQGSFRKASEALHVSASSIDRQILRVEQELAMPLFERHPTGLRLTAAGELLLHAANNWKKDFTRV
CEQLDDLRGLRRGHVRIATIDAINRHFFSTMLKEVHKHYPNISFTLTTINNIHIQQALISGEADFGIMLNPQTSRELQVR
AFAEMNMGIVVPTGHPLASRSAVRFSQCLDYPFILPSAPLMISEPVEALVNISGNEVKEVAVSNNIHMIRTLIKEQMGIG
ILCRLDILDEIESGQLAFVPLTDPQLKPFTLALCVSPARQLSLAASMMLNQLEMLFSQL
>Mature_299_residues
MFAFSRFLLYFTEVARQGSFRKASEALHVSASSIDRQILRVEQELAMPLFERHPTGLRLTAAGELLLHAANNWKKDFTRV
CEQLDDLRGLRRGHVRIATIDAINRHFFSTMLKEVHKHYPNISFTLTTINNIHIQQALISGEADFGIMLNPQTSRELQVR
AFAEMNMGIVVPTGHPLASRSAVRFSQCLDYPFILPSAPLMISEPVEALVNISGNEVKEVAVSNNIHMIRTLIKEQMGIG
ILCRLDILDEIESGQLAFVPLTDPQLKPFTLALCVSPARQLSLAASMMLNQLEMLFSQL

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=231, Percent_Identity=30.7359307359307, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI145693105, Length=184, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787601, Length=189, Percent_Identity=28.042328042328, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788887, Length=265, Percent_Identity=25.2830188679245, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788748, Length=162, Percent_Identity=28.3950617283951, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=22.6765799256506, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=214, Percent_Identity=25.7009345794392, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082132, Length=297, Percent_Identity=24.2424242424242, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788481, Length=265, Percent_Identity=26.4150943396226, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=249, Percent_Identity=21.285140562249, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786508, Length=179, Percent_Identity=27.9329608938547, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787949, Length=171, Percent_Identity=28.0701754385965, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790208, Length=149, Percent_Identity=29.5302013422819, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33582; Mature: 33582

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAFSRFLLYFTEVARQGSFRKASEALHVSASSIDRQILRVEQELAMPLFERHPTGLRLT
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEE
AAGELLLHAANNWKKDFTRVCEQLDDLRGLRRGHVRIATIDAINRHFFSTMLKEVHKHYP
EHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCC
NISFTLTTINNIHIQQALISGEADFGIMLNPQTSRELQVRAFAEMNMGIVVPTGHPLASR
CCEEEEEEECCHHHHHHHHCCCCCCEEEECCCCCCHHEEEEEEECCCCEEEECCCCCCCH
SAVRFSQCLDYPFILPSAPLMISEPVEALVNISGNEVKEVAVSNNIHMIRTLIKEQMGIG
HHHHHHHHCCCCEECCCCCCEEHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCE
ILCRLDILDEIESGQLAFVPLTDPQLKPFTLALCVSPARQLSLAASMMLNQLEMLFSQL
EEEEEEHHHCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFAFSRFLLYFTEVARQGSFRKASEALHVSASSIDRQILRVEQELAMPLFERHPTGLRLT
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEE
AAGELLLHAANNWKKDFTRVCEQLDDLRGLRRGHVRIATIDAINRHFFSTMLKEVHKHYP
EHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCC
NISFTLTTINNIHIQQALISGEADFGIMLNPQTSRELQVRAFAEMNMGIVVPTGHPLASR
CCEEEEEEECCHHHHHHHHCCCCCCEEEECCCCCCHHEEEEEEECCCCEEEECCCCCCCH
SAVRFSQCLDYPFILPSAPLMISEPVEALVNISGNEVKEVAVSNNIHMIRTLIKEQMGIG
HHHHHHHHCCCCEECCCCCCEEHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCE
ILCRLDILDEIESGQLAFVPLTDPQLKPFTLALCVSPARQLSLAASMMLNQLEMLFSQL
EEEEEEHHHCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]