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The map label for this gene is appA [H]

Identifier: 238895063

GI number: 238895063

Start: 2945215

End: 2946918

Strand: Direct

Name: appA [H]

Synonym: KP1_3095

Alternate gene names: 238895063

Gene position: 2945215-2946918 (Clockwise)

Preceding gene: 238895062

Following gene: 238895064

Centisome position: 56.12

GC content: 58.8

Gene sequence:

>1704_bases
TTGTCCAGATTACAACAAGGATTAAAAACCAGACAAACCCGCTTCGCCCTGGCGCTAAAAGTGGCCCTGCTCGGCGGCGT
GGTAGCATTTTCTTCTGTTGCCCATGCGGAAGTGATCCTGAAAGAAGGCATCACCCCGGCCAGCGACGCCAGCCAGATCC
CGGCCAGCGCCAAACTGCGCACAGACACGGTGGTGGCCGGGATCTCCGAGCCGCAGGGGATCTTTAACCCCTACTTTTTC
ATCAACGGCTGGGATGAGAACGTGACTAACGTCATTTTTTCACGCCTGATCGACTGGGACAGCCAGGGCAAGCTGGTCCC
GGGACTGGCGGAGAGCTGGACGGTCAGCCCGGATAACAAAGTCTATACCATCAAATTGCGCCCTGGTCTCACCTTTAGCG
ACGGCTCACCGCTGACGGCGGAAGATGTCGCCTTTACCCTGACGGTCCTGCTCGACCCCAGCTATGATGGCGATACCGAC
ATCACCCTGGCCAATATCGCCGGCGGGGCGGACTACAAAGCCGGCAAGGCTGACAGCGTCAGCGGCCTGAAGGTCATCGA
CCCGCTCACCCTGCAGGTGACCACCACGCAGCCAGGCGCCACCACGCTGGCCAAAATTGGCGGGCCTGTACTGTCGAAAG
CCTGGTACGGCAAAGGGTATCAGCGGGGGAATCTCGACTATCTGCGCTCACTGCACGGCAAGCCGCTGGGCAACGGCCCT
TACGTGTACGACAAATATATTCCCGGTCAGGAAATACGCTTCCATGCCAATAGCCATTTTTACCGTGGGACGCCGCCAAC
GCCGCGCTTTATCTATCGCGTGACCAATCCGTCCACCAACTTCCAGCTGTTCCAGACCGGGGAGACCGACTACGACGCCT
TTACCTCGCGCCCCGACGATATTGAGCAACTGAAGATGCTCGGCTTCGCCAATATTAATCTTTACGGCTCCAGCGACTAC
AGCCAGGTGGAATTCAACGTGCATCGCCCGGCGCTACAGGATAAGCGGGTGCGCCAGGCGCTGATTTACGGCCTGGATAG
GCAGAAGCTGATCGACGTGGTCTATCAAGGATACGGCAAAGTCGCCATCGAACCGATCGCGCCGATCTCCTGGGCCTTTA
ACGCTGAAGGCGTCAACCCTTACCCTTACGATCCGGCACAGGCCAAAAAGCTGCTGGATGAGGCCGGCTGGAAACCAGGC
GCCGACGGTATCCGCGCCAAAGACGGCCAGCGCCTGGAGCTGACCCTGCTGGTGAGCAAGAAGGTGCTCAACGATGCGCT
GATCCCCATCGCCAAGGAGAACTGGCGGCAGATTGGCGTGCTGCTGAAGCCGCAGGTGGTGGATTTCAACGCCCTGATGG
CGCAGCGCAAAGCCGGAAATTACGATTTGGCCTCGTTCAGCACCAGCACCCTCAACGACCCGCACGACGGGGTATGGGAT
TTCTACAGCAGCGAAGCTAAAGAGTCCGGCTACCATAACGCGGAAGTCGATAAGCTGATTAACGCCGGCAACGCGGTGCT
CGACATCGAACAGCGCAAGCCGATCTACCACCAGCTGTATAAAGTGCTGGCCGACGATCCGCCGGTGATCCTGCTCGGCT
ATCGCGAGATCCTTTCCGCCAGCAGCGCCCGCGTCAGCGGCTTTAAGCCGGATATCTACAACGGCCTGACCGGCAGCCTG
CCGGATGTAAAAATCGTCAAATAA

Upstream 100 bases:

>100_bases
ATAAATCATAGCGTTTCAGTGTTAGACAGGGCGAAGTGCGCCTCTTATCTTTCGTCATGAATTGTAAATAACCACGCAAT
CATTTGATGAGGTAGCACTT

Downstream 100 bases:

>100_bases
ATCCCTGGCCGCCGGGTTAACCGACGGCTGATGTGAGACCGTCATGAGAAATTTCATCCTGAGGCGCCTGCTGCAAACGC
TGCCGATACTGCTGCTGGCC

Product: oligopeptide ABC transport system oligopeptide-binding component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSRLQQGLKTRQTRFALALKVALLGGVVAFSSVAHAEVILKEGITPASDASQIPASAKLRTDTVVAGISEPQGIFNPYFF
INGWDENVTNVIFSRLIDWDSQGKLVPGLAESWTVSPDNKVYTIKLRPGLTFSDGSPLTAEDVAFTLTVLLDPSYDGDTD
ITLANIAGGADYKAGKADSVSGLKVIDPLTLQVTTTQPGATTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGP
YVYDKYIPGQEIRFHANSHFYRGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDDIEQLKMLGFANINLYGSSDY
SQVEFNVHRPALQDKRVRQALIYGLDRQKLIDVVYQGYGKVAIEPIAPISWAFNAEGVNPYPYDPAQAKKLLDEAGWKPG
ADGIRAKDGQRLELTLLVSKKVLNDALIPIAKENWRQIGVLLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWD
FYSSEAKESGYHNAEVDKLINAGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSASSARVSGFKPDIYNGLTGSL
PDVKIVK

Sequences:

>Translated_567_residues
MSRLQQGLKTRQTRFALALKVALLGGVVAFSSVAHAEVILKEGITPASDASQIPASAKLRTDTVVAGISEPQGIFNPYFF
INGWDENVTNVIFSRLIDWDSQGKLVPGLAESWTVSPDNKVYTIKLRPGLTFSDGSPLTAEDVAFTLTVLLDPSYDGDTD
ITLANIAGGADYKAGKADSVSGLKVIDPLTLQVTTTQPGATTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGP
YVYDKYIPGQEIRFHANSHFYRGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDDIEQLKMLGFANINLYGSSDY
SQVEFNVHRPALQDKRVRQALIYGLDRQKLIDVVYQGYGKVAIEPIAPISWAFNAEGVNPYPYDPAQAKKLLDEAGWKPG
ADGIRAKDGQRLELTLLVSKKVLNDALIPIAKENWRQIGVLLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWD
FYSSEAKESGYHNAEVDKLINAGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSASSARVSGFKPDIYNGLTGSL
PDVKIVK
>Mature_566_residues
SRLQQGLKTRQTRFALALKVALLGGVVAFSSVAHAEVILKEGITPASDASQIPASAKLRTDTVVAGISEPQGIFNPYFFI
NGWDENVTNVIFSRLIDWDSQGKLVPGLAESWTVSPDNKVYTIKLRPGLTFSDGSPLTAEDVAFTLTVLLDPSYDGDTDI
TLANIAGGADYKAGKADSVSGLKVIDPLTLQVTTTQPGATTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGPY
VYDKYIPGQEIRFHANSHFYRGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDDIEQLKMLGFANINLYGSSDYS
QVEFNVHRPALQDKRVRQALIYGLDRQKLIDVVYQGYGKVAIEPIAPISWAFNAEGVNPYPYDPAQAKKLLDEAGWKPGA
DGIRAKDGQRLELTLLVSKKVLNDALIPIAKENWRQIGVLLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWDF
YSSEAKESGYHNAEVDKLINAGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSASSARVSGFKPDIYNGLTGSLP
DVKIVK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=537, Percent_Identity=25.6983240223464, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787052, Length=546, Percent_Identity=25.0915750915751, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789397, Length=476, Percent_Identity=26.0504201680672, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1787762, Length=517, Percent_Identity=26.6924564796905, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1789887, Length=485, Percent_Identity=24.5360824742268, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787551, Length=365, Percent_Identity=26.3013698630137, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081878, Length=461, Percent_Identity=24.5119305856833, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787495, Length=555, Percent_Identity=24.5045045045045, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62269; Mature: 62138

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLQQGLKTRQTRFALALKVALLGGVVAFSSVAHAEVILKEGITPASDASQIPASAKLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEE
TDTVVAGISEPQGIFNPYFFINGWDENVTNVIFSRLIDWDSQGKLVPGLAESWTVSPDNK
CCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCE
VYTIKLRPGLTFSDGSPLTAEDVAFTLTVLLDPSYDGDTDITLANIAGGADYKAGKADSV
EEEEEECCCEEECCCCCCCHHHEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCC
SGLKVIDPLTLQVTTTQPGATTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGP
CCEEEECCEEEEEEECCCCCHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
YVYDKYIPGQEIRFHANSHFYRGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDD
EEEECCCCCCCEEEECCCCEECCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHCCCCCH
IEQLKMLGFANINLYGSSDYSQVEFNVHRPALQDKRVRQALIYGLDRQKLIDVVYQGYGK
HHHHHHHCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
VAIEPIAPISWAFNAEGVNPYPYDPAQAKKLLDEAGWKPGADGIRAKDGQRLELTLLVSK
EEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEH
KVLNDALIPIAKENWRQIGVLLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWD
HHHHHHCCCCCCCCHHHCCEEECCEEECHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHH
FYSSEAKESGYHNAEVDKLINAGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSA
HHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECHHHHCC
SSARVSGFKPDIYNGLTGSLPDVKIVK
CCCCCCCCCCHHHCCCCCCCCCEEEEC
>Mature Secondary Structure 
SRLQQGLKTRQTRFALALKVALLGGVVAFSSVAHAEVILKEGITPASDASQIPASAKLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEE
TDTVVAGISEPQGIFNPYFFINGWDENVTNVIFSRLIDWDSQGKLVPGLAESWTVSPDNK
CCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCE
VYTIKLRPGLTFSDGSPLTAEDVAFTLTVLLDPSYDGDTDITLANIAGGADYKAGKADSV
EEEEEECCCEEECCCCCCCHHHEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCC
SGLKVIDPLTLQVTTTQPGATTLAKIGGPVLSKAWYGKGYQRGNLDYLRSLHGKPLGNGP
CCEEEECCEEEEEEECCCCCHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
YVYDKYIPGQEIRFHANSHFYRGTPPTPRFIYRVTNPSTNFQLFQTGETDYDAFTSRPDD
EEEECCCCCCCEEEECCCCEECCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHCCCCCH
IEQLKMLGFANINLYGSSDYSQVEFNVHRPALQDKRVRQALIYGLDRQKLIDVVYQGYGK
HHHHHHHCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
VAIEPIAPISWAFNAEGVNPYPYDPAQAKKLLDEAGWKPGADGIRAKDGQRLELTLLVSK
EEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEH
KVLNDALIPIAKENWRQIGVLLKPQVVDFNALMAQRKAGNYDLASFSTSTLNDPHDGVWD
HHHHHHCCCCCCCCHHHCCEEECCEEECHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHH
FYSSEAKESGYHNAEVDKLINAGNAVLDIEQRKPIYHQLYKVLADDPPVILLGYREILSA
HHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECHHHHCC
SSARVSGFKPDIYNGLTGSLPDVKIVK
CCCCCCCCCCHHHCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]