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The map label for this gene is nemA [H]

Identifier: 238895032

GI number: 238895032

Start: 2917070

End: 2918167

Strand: Direct

Name: nemA [H]

Synonym: KP1_3062

Alternate gene names: 238895032

Gene position: 2917070-2918167 (Clockwise)

Preceding gene: 238895031

Following gene: 238895033

Centisome position: 55.58

GC content: 61.38

Gene sequence:

>1098_bases
ATGTCGGAAGCAAAATTATTCAGCCCACTGAAAGTCGGTGCGGTCACCGTCCCTAATCGCGTATTTATGGCGCCGTTGAC
GCGTCTGCGCAGCATTGAGCCGGGCGATATCCCTACTCCGCTGATGGGCGAATACTACCGCCAGCGCGCCAGTTCCGGGC
TGATCATCACCGAAGCGACGCAGATCTCCGCGCAGGCCAAAGGCTACGCCGGCGCGCCTGGGCTGCACAGCCCGGAGCAG
ATTGCCGCCTGGCAGAAAATCACCGCCGGCGTTCATGCCGAAAACGGTCATATCGCCGTCCAGCTGTGGCACACCGGGCG
TATTTCCCATAGCAGCCTGCAGCCTGGCGGCGCCGCCCCGGTAGCGCCTTCCGCGCTGAGCGCGGGCACCCGTACCTCAC
TGCGTGACGAAAACGGCCACGCCATTCGCGTCGACACCTCAATGCCCCGCGCCCTGGAAACCGCAGAGATCCCAGGGATC
GTCAACGATTTCCGTCAGGCGGTCGGCAATGCCCGTGACGCCGGCTTCGATCTGGTTGAGCTGCACTCAGCCCACGGCTA
TCTGCTGCACCAGTTCCTCTCGCCATCGGCGAACCAGCGTACCGATCAGTACGGCGGCAGCGTGGAGAACCGTGCCCGTC
TGGTGCTGGAAGTGGTTGACGCCGTGAGTCAAGAGTGGAGCGCCGAACGCATTGGCATCCGCGTGTCGCCGATTGGCAGC
TTCCAGAATGTGGATAATGGCCCGAACGAAGAAGAAGACGCGCTGTATCTGATTAGCGAGCTGGCGAAACGCGGTATCGC
CTATCTGCACATGTCCGAGCCGGACTGGGCGGGCGGCAAACCTTACAGTGAAGCTTTCCGTCAGAAAGTCCGCGACCGCT
TCCCGGGGGTGATCATTGGCGCTGGCGCTTACACAGTAGAAAAAGCCAACGATCTTATCAATAAAGGGCTGATTGATGCC
GTGGCCTTTGGCCGCGATTACATCGCCAACCCGGATCTGGTGGCTCGCCTGCAGAAAAAAGCGCCGCTTAACCCGCAACG
CCCGGAATCCTTCTACGGCGGCGGCGCGGAAGGCTACACCGATTACCCGACTCTGTAA

Upstream 100 bases:

>100_bases
GCGCCCTTGCCCACGCCAAACAGATTATTGCGGCGCCTGCGGTATAACAGGCGTTTTTATTTCATCAGTTACTAGACGAC
CGGTCTACTCAGGAGTCACT

Downstream 100 bases:

>100_bases
TCCCTCCATTGATAGCGGCGACGTATCGCCGCTATACTCAACGCTCTGATCTATCAAATAAACTGCATAGGGGACATTAT
GCGCTTACTCCACACCATGC

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSEAKLFSPLKVGAVTVPNRVFMAPLTRLRSIEPGDIPTPLMGEYYRQRASSGLIITEATQISAQAKGYAGAPGLHSPEQ
IAAWQKITAGVHAENGHIAVQLWHTGRISHSSLQPGGAAPVAPSALSAGTRTSLRDENGHAIRVDTSMPRALETAEIPGI
VNDFRQAVGNARDAGFDLVELHSAHGYLLHQFLSPSANQRTDQYGGSVENRARLVLEVVDAVSQEWSAERIGIRVSPIGS
FQNVDNGPNEEEDALYLISELAKRGIAYLHMSEPDWAGGKPYSEAFRQKVRDRFPGVIIGAGAYTVEKANDLINKGLIDA
VAFGRDYIANPDLVARLQKKAPLNPQRPESFYGGGAEGYTDYPTL

Sequences:

>Translated_365_residues
MSEAKLFSPLKVGAVTVPNRVFMAPLTRLRSIEPGDIPTPLMGEYYRQRASSGLIITEATQISAQAKGYAGAPGLHSPEQ
IAAWQKITAGVHAENGHIAVQLWHTGRISHSSLQPGGAAPVAPSALSAGTRTSLRDENGHAIRVDTSMPRALETAEIPGI
VNDFRQAVGNARDAGFDLVELHSAHGYLLHQFLSPSANQRTDQYGGSVENRARLVLEVVDAVSQEWSAERIGIRVSPIGS
FQNVDNGPNEEEDALYLISELAKRGIAYLHMSEPDWAGGKPYSEAFRQKVRDRFPGVIIGAGAYTVEKANDLINKGLIDA
VAFGRDYIANPDLVARLQKKAPLNPQRPESFYGGGAEGYTDYPTL
>Mature_364_residues
SEAKLFSPLKVGAVTVPNRVFMAPLTRLRSIEPGDIPTPLMGEYYRQRASSGLIITEATQISAQAKGYAGAPGLHSPEQI
AAWQKITAGVHAENGHIAVQLWHTGRISHSSLQPGGAAPVAPSALSAGTRTSLRDENGHAIRVDTSMPRALETAEIPGIV
NDFRQAVGNARDAGFDLVELHSAHGYLLHQFLSPSANQRTDQYGGSVENRARLVLEVVDAVSQEWSAERIGIRVSPIGSF
QNVDNGPNEEEDALYLISELAKRGIAYLHMSEPDWAGGKPYSEAFRQKVRDRFPGVIIGAGAYTVEKANDLINKGLIDAV
AFGRDYIANPDLVARLQKKAPLNPQRPESFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=86.5753424657534, Blast_Score=655, Evalue=0.0,
Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=31.0541310541311, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=356, Percent_Identity=30.6179775280899, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=351, Percent_Identity=27.9202279202279, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=212, Percent_Identity=33.0188679245283, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=351, Percent_Identity=27.0655270655271, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=196, Percent_Identity=33.6734693877551, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=28.3582089552239, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=86, Percent_Identity=39.5348837209302, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=383, Percent_Identity=37.3368146214099, Blast_Score=232, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6321973, Length=383, Percent_Identity=37.3368146214099, Blast_Score=219, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39403; Mature: 39271

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKLFSPLKVGAVTVPNRVFMAPLTRLRSIEPGDIPTPLMGEYYRQRASSGLIITEAT
CCCCCCCCCCEECEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECH
QISAQAKGYAGAPGLHSPEQIAAWQKITAGVHAENGHIAVQLWHTGRISHSSLQPGGAAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCEEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGHAIRVDTSMPRALETAEIPGIVNDFRQAVGNARDAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCEE
LHSAHGYLLHQFLSPSANQRTDQYGGSVENRARLVLEVVDAVSQEWSAERIGIRVSPIGS
HHCCCHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCEEEECCCC
FQNVDNGPNEEEDALYLISELAKRGIAYLHMSEPDWAGGKPYSEAFRQKVRDRFPGVIIG
CCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEE
AGAYTVEKANDLINKGLIDAVAFGRDYIANPDLVARLQKKAPLNPQRPESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure 
SEAKLFSPLKVGAVTVPNRVFMAPLTRLRSIEPGDIPTPLMGEYYRQRASSGLIITEAT
CCCCCCCCCEECEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECH
QISAQAKGYAGAPGLHSPEQIAAWQKITAGVHAENGHIAVQLWHTGRISHSSLQPGGAAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCEEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGHAIRVDTSMPRALETAEIPGIVNDFRQAVGNARDAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCEE
LHSAHGYLLHQFLSPSANQRTDQYGGSVENRARLVLEVVDAVSQEWSAERIGIRVSPIGS
HHCCCHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCEEEECCCC
FQNVDNGPNEEEDALYLISELAKRGIAYLHMSEPDWAGGKPYSEAFRQKVRDRFPGVIIG
CCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEE
AGAYTVEKANDLINKGLIDAVAFGRDYIANPDLVARLQKKAPLNPQRPESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]