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The map label for this gene is rnfB [H]

Identifier: 238895008

GI number: 238895008

Start: 2895072

End: 2895650

Strand: Direct

Name: rnfB [H]

Synonym: KP1_3037

Alternate gene names: 238895008

Gene position: 2895072-2895650 (Clockwise)

Preceding gene: 238895007

Following gene: 238895009

Centisome position: 55.16

GC content: 59.93

Gene sequence:

>579_bases
ATGAGCGCAGTCTGGATAGCCGTGATCGCCATCAGCCTGTTAGGGCTGATTTTCGGCTTAATACTGGGATACGCCTCGCG
TCGTTTCGCGGTGCAGGACGACCCGGTGGTGGAAAAAATTGATGAGCTATTGCCGCAGAGCCAGTGTGGGCAGTGCGGGT
ACCCTGGGTGTCGCCCCTATGCGGAAGCAGTCGGCGTGCAGGGTGAGAAAATAAACCGCTGCGCGCCGGGTGGCGAAGCG
GTGATGTTAAAAATCGCCGCCTTGCTGAACGTCGACCCGCAGCCTGTCGACGGCGACGCACAGGAAGCGGAGCCGGCCCG
CATGCTCGCGGTAATTGACGAGCCGAACTGCATTGGCTGTACCAAGTGTATCCAGGCCTGTCCGGTGGATGCCATCGTTG
GGGCGACCCGCGCCATGCATACCGTGATGAGCGACCTGTGCACCGGCTGCAATTTGTGCGTCGCCCCCTGCCCGACCCAG
TGCATTTCACTCGTTCCGGTGGCCACCACCCCGGAGACCTGGAAATGGGATCTGCATACGATCCCGGTGCGAAATATTCC
TGTGGAACAACATGTTTAA

Upstream 100 bases:

>100_bases
CCGATGTCCCCGCGCCGTTTCGCGGTAATGCCATCGCGCTGATTACCGCCGGTTTAATGTCTCTGGCCTTCATGGGCTTT
AGTGGTTTGGTGAAGTTGTA

Downstream 100 bases:

>100_bases
GCTATTTTCCGCTTTCCGAAAAGACAAGGTCTGGGATTTCAACGGCGGTATTCACCCGCCTGAAATGAAAACCCAGTCTA
ACGGCACCCCGCTGCGTCAG

Product: electron transport complex protein RnfB

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSAVWIAVIAISLLGLIFGLILGYASRRFAVQDDPVVEKIDELLPQSQCGQCGYPGCRPYAEAVGVQGEKINRCAPGGEA
VMLKIAALLNVDPQPVDGDAQEAEPARMLAVIDEPNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVAPCPTQ
CISLVPVATTPETWKWDLHTIPVRNIPVEQHV

Sequences:

>Translated_192_residues
MSAVWIAVIAISLLGLIFGLILGYASRRFAVQDDPVVEKIDELLPQSQCGQCGYPGCRPYAEAVGVQGEKINRCAPGGEA
VMLKIAALLNVDPQPVDGDAQEAEPARMLAVIDEPNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVAPCPTQ
CISLVPVATTPETWKWDLHTIPVRNIPVEQHV
>Mature_191_residues
SAVWIAVIAISLLGLIFGLILGYASRRFAVQDDPVVEKIDELLPQSQCGQCGYPGCRPYAEAVGVQGEKINRCAPGGEAV
MLKIAALLNVDPQPVDGDAQEAEPARMLAVIDEPNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVAPCPTQC
ISLVPVATTPETWKWDLHTIPVRNIPVEQHV

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=191, Percent_Identity=84.8167539267016, Blast_Score=337, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 20452; Mature: 20321

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVWIAVIAISLLGLIFGLILGYASRRFAVQDDPVVEKIDELLPQSQCGQCGYPGCRPY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCHH
AEAVGVQGEKINRCAPGGEAVMLKIAALLNVDPQPVDGDAQEAEPARMLAVIDEPNCIGC
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
TKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVAPCPTQCISLVPVATTPETWKWDLHT
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCCEECEEE
IPVRNIPVEQHV
CCCCCCCHHCCC
>Mature Secondary Structure 
SAVWIAVIAISLLGLIFGLILGYASRRFAVQDDPVVEKIDELLPQSQCGQCGYPGCRPY
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCHH
AEAVGVQGEKINRCAPGGEAVMLKIAALLNVDPQPVDGDAQEAEPARMLAVIDEPNCIGC
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
TKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVAPCPTQCISLVPVATTPETWKWDLHT
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCCEECEEE
IPVRNIPVEQHV
CCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA