Click here to switch to the map view.

The map label for this gene is narV [H]

Identifier: 238894926

GI number: 238894926

Start: 2815597

End: 2816277

Strand: Direct

Name: narV [H]

Synonym: KP1_2949

Alternate gene names: 238894926

Gene position: 2815597-2816277 (Clockwise)

Preceding gene: 238894925

Following gene: 238894931

Centisome position: 53.65

GC content: 59.77

Gene sequence:

>681_bases
ATGATCCAGTATCTGAACGTCTTCTTTTACGATATCTACCCTTACCTGTGCGGCACCGTCTTTCTGGTGGGCAGCTGGCT
GCGCTACGATTACGGGCAGTATACCTGGCGCGCCTCATCCAGCCAGATGCTGGATAAGCGCGGGATGGTGCTATGGTCGA
ACTTATTTCATATCGGCATTCTCGGCATCTTCTTTGGCCATCTCTTTGGCATGCTGACCCCACACTGGGTCTACTCGTGG
TTCCTGCCGATGTCCCAGAAGCAGCTGATGGCCATGATCCTTGGCGGCGTATGCGGCGTGCTGACCCTGGTAGGCGGTAT
CGGCCTGCTGATGCGCCGCCTGACCAACCCGCGAATTCGCGCCACCTCCACGACCGCGGATATTTTGATCCTGTGCATTC
TGCTGATTCAGTGCGCGCTGGGGCTGACGACCATCCCCTTCTCCGCCCAGCATCCGGACGGCAGCGAAATGCTCAAGCTG
GTGGACTGGGCGCAGGCGGTGGTCACCTTCCACGGCGGCGCCTCGGCGCATCTGGACGGTGTCGCATGGATTTACCGCGT
CCACCTGGTGCTCGGAATGACCATCTTTCTGATCTTCCCCTTCACCCGCCTGGTTCACGTCTGGAGCGCGCCGGTGGAAT
ATTTCACCCGGCGTTACCAGGTGGTACGCTCCCGGCGCTGA

Upstream 100 bases:

>100_bases
ATGCGACCGCTTGCGATAGCTCGTCGATGCAAGCTTATCAACGACGCTTTAGTCAGGATGTGGCGCCGCAGTACGTTGAC
ATCCGCGCCGGAGGCCCGAA

Downstream 100 bases:

>100_bases
GCGCTGGCGGCGTTTACCATTCATCCTTACCGGTAGCCCGGACGAGGCCTTCGCGCCAAGTTCGGGTATTTATCGTACCT
GGCTCCAGGCTTTATGCCGG

Product: cryptic nitrate reductase 2 gamma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MIQYLNVFFYDIYPYLCGTVFLVGSWLRYDYGQYTWRASSSQMLDKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWVYSW
FLPMSQKQLMAMILGGVCGVLTLVGGIGLLMRRLTNPRIRATSTTADILILCILLIQCALGLTTIPFSAQHPDGSEMLKL
VDWAQAVVTFHGGASAHLDGVAWIYRVHLVLGMTIFLIFPFTRLVHVWSAPVEYFTRRYQVVRSRR

Sequences:

>Translated_226_residues
MIQYLNVFFYDIYPYLCGTVFLVGSWLRYDYGQYTWRASSSQMLDKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWVYSW
FLPMSQKQLMAMILGGVCGVLTLVGGIGLLMRRLTNPRIRATSTTADILILCILLIQCALGLTTIPFSAQHPDGSEMLKL
VDWAQAVVTFHGGASAHLDGVAWIYRVHLVLGMTIFLIFPFTRLVHVWSAPVEYFTRRYQVVRSRR
>Mature_226_residues
MIQYLNVFFYDIYPYLCGTVFLVGSWLRYDYGQYTWRASSSQMLDKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWVYSW
FLPMSQKQLMAMILGGVCGVLTLVGGIGLLMRRLTNPRIRATSTTADILILCILLIQCALGLTTIPFSAQHPDGSEMLKL
VDWAQAVVTFHGGASAHLDGVAWIYRVHLVLGMTIFLIFPFTRLVHVWSAPVEYFTRRYQVVRSRR

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transf

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=226, Percent_Identity=82.3008849557522, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1787480, Length=224, Percent_Identity=73.6607142857143, Blast_Score=353, Evalue=6e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25836; Mature: 25836

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQYLNVFFYDIYPYLCGTVFLVGSWLRYDYGQYTWRASSSQMLDKRGMVLWSNLFHIGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCHHHHHHHHHHHH
LGIFFGHLFGMLTPHWVYSWFLPMSQKQLMAMILGGVCGVLTLVGGIGLLMRRLTNPRIR
HHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ATSTTADILILCILLIQCALGLTTIPFSAQHPDGSEMLKLVDWAQAVVTFHGGASAHLDG
ECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
VAWIYRVHLVLGMTIFLIFPFTRLVHVWSAPVEYFTRRYQVVRSRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIQYLNVFFYDIYPYLCGTVFLVGSWLRYDYGQYTWRASSSQMLDKRGMVLWSNLFHIGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCHHHHHHHHHHHH
LGIFFGHLFGMLTPHWVYSWFLPMSQKQLMAMILGGVCGVLTLVGGIGLLMRRLTNPRIR
HHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ATSTTADILILCILLIQCALGLTTIPFSAQHPDGSEMLKLVDWAQAVVTFHGGASAHLDG
ECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
VAWIYRVHLVLGMTIFLIFPFTRLVHVWSAPVEYFTRRYQVVRSRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]