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The map label for this gene is narU [H]

Identifier: 238894922

GI number: 238894922

Start: 2808127

End: 2809509

Strand: Direct

Name: narU [H]

Synonym: KP1_2945

Alternate gene names: 238894922

Gene position: 2808127-2809509 (Clockwise)

Preceding gene: 238894921

Following gene: 238894923

Centisome position: 53.5

GC content: 55.68

Gene sequence:

>1383_bases
ATGTCGGTGCAGAATGATAAAGATAATCATTACCTTTTGAACAACTGGCGGCCTGAAAACAAGGCGTTCTGGGAAAATAA
AGGCAAAGCCATTGCGCGAAGAAATCTATGGATATCAGTGACCTGTCTGCTACTTGCTTTCTGCGTATGGATGTTATTCA
GCGCCGTAGCGGTGAATCTCAATAAGGTCGGTTTTCATTTCACCACCGACCAGCTTTTTTTATTAACCGCCCTGCCTTCA
CTTTCCGGCGCGATATTACGGGTCCCCTACTCCTTTATGGTACCCCTTTTTGGGGGGCGCTACTGGACCGTATTAAGTAC
CGTTATCCTGATCGTGCCTTGTATTTGGTTAGGGGTGGCAATCCAGAATATTACTACTCCGTTCTGGGTATTTATTATTA
TTGCGCTGCTGTGCGGTTTTGCCGGAGCGAATTTTGCTTCCAGCATGGGAAATATCAGTTTCTTTTTCCCCAAAGCGAAA
CAGGGCAGCGCGCTGGGAGTCAACGGCGGCCTTGGCAATCTGGGCGTAAGCGTGATGCAGATGGTCGCCCCGGCGGTCAT
CTTCCTGCCGCTCTTCACCTTTCTCGGCGTCCATGGCGTCACCCAGCCCGATGGTTCGACAATCACCCTGAGTAATGCGG
CGCTCGTCTGGGTACCGCTGCTGCTGCTCGCCACCGTCGCGGCATGGTTCGGCATGAACGATATCGCCGGTTCAAAGGCC
TCGATTCGCGATCAGCTTCCCGTGCTCAAGCGACCGCATATGTGGCTGTTAAGCCTGCTCTATCTGGCGACGTTTGGCTC
GTTCATCGGCTTTTCCGCCGGCTTTGCCATGCTGGCGAAAACCCAGTTTCCTGCGGTCGATATCCTCAAGCTCGCCTTCT
TTGGACCGTTCATCGGCGCGCTGGCGCGCTCGTTCGGCGGGATCATCTCCGACCGTCTCGGCGGCGTGCGGGTGACGCTG
GTTAACTTCGTCCTGATGGCGCTGTTTACCGGCCTGCTGTTTCTGACACTGCCAGGCAGCGGCTCCGGCAGCTTTCTCGC
TTTCTACGTGGTGTTTATGGGGCTCTTTTTAACCGCCGGTCTGGGAAGCGGCTCCACCTTCCAGATGATCGCCGTCATCT
TCCGCCAGCTCACCATCGACAGCGTAAAACAGCGCGGCGGCAGCGATGAAGAAGCGCAGCACGAGGCGGTGACCGATACC
GCTGCCGCGCTGGGCTTTATCTCGGCGATCGGCGCCATCGGCGGCTTCTTTATTCCGAAAGCCTTCGGCACCTCGCTGGC
GATGACCGGCTCACCGGTTGGCGCGATGAAAGTCTTTTTTGTCTTCTATGTGGTTTGCGTGCTGGTGACCTGGCTGGTCT
ACGGCCGCCGTAAATCCGCTTAA

Upstream 100 bases:

>100_bases
ACGCTTCATAGATAACGCAAATTAAGCGTTATTCACTATCCCCTTGTATTCAGCTGTTGACCTTATTTATTTACTCCTTT
CCGGGCAAGTAGAGGGCAAT

Downstream 100 bases:

>100_bases
CCATAACGATTTATCTGCCGGATAGCGGCTTACGCCTTATCCGGCCTTACGAGTTCGTTCGCTCATCCGGCCGGGTGGCA
CGATGCCGGACATCATCGGA

Product: nitrate extrusion protein

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator 2 [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSVQNDKDNHYLLNNWRPENKAFWENKGKAIARRNLWISVTCLLLAFCVWMLFSAVAVNLNKVGFHFTTDQLFLLTALPS
LSGAILRVPYSFMVPLFGGRYWTVLSTVILIVPCIWLGVAIQNITTPFWVFIIIALLCGFAGANFASSMGNISFFFPKAK
QGSALGVNGGLGNLGVSVMQMVAPAVIFLPLFTFLGVHGVTQPDGSTITLSNAALVWVPLLLLATVAAWFGMNDIAGSKA
SIRDQLPVLKRPHMWLLSLLYLATFGSFIGFSAGFAMLAKTQFPAVDILKLAFFGPFIGALARSFGGIISDRLGGVRVTL
VNFVLMALFTGLLFLTLPGSGSGSFLAFYVVFMGLFLTAGLGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEAQHEAVTDT
AAALGFISAIGAIGGFFIPKAFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWLVYGRRKSA

Sequences:

>Translated_460_residues
MSVQNDKDNHYLLNNWRPENKAFWENKGKAIARRNLWISVTCLLLAFCVWMLFSAVAVNLNKVGFHFTTDQLFLLTALPS
LSGAILRVPYSFMVPLFGGRYWTVLSTVILIVPCIWLGVAIQNITTPFWVFIIIALLCGFAGANFASSMGNISFFFPKAK
QGSALGVNGGLGNLGVSVMQMVAPAVIFLPLFTFLGVHGVTQPDGSTITLSNAALVWVPLLLLATVAAWFGMNDIAGSKA
SIRDQLPVLKRPHMWLLSLLYLATFGSFIGFSAGFAMLAKTQFPAVDILKLAFFGPFIGALARSFGGIISDRLGGVRVTL
VNFVLMALFTGLLFLTLPGSGSGSFLAFYVVFMGLFLTAGLGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEAQHEAVTDT
AAALGFISAIGAIGGFFIPKAFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWLVYGRRKSA
>Mature_459_residues
SVQNDKDNHYLLNNWRPENKAFWENKGKAIARRNLWISVTCLLLAFCVWMLFSAVAVNLNKVGFHFTTDQLFLLTALPSL
SGAILRVPYSFMVPLFGGRYWTVLSTVILIVPCIWLGVAIQNITTPFWVFIIIALLCGFAGANFASSMGNISFFFPKAKQ
GSALGVNGGLGNLGVSVMQMVAPAVIFLPLFTFLGVHGVTQPDGSTITLSNAALVWVPLLLLATVAAWFGMNDIAGSKAS
IRDQLPVLKRPHMWLLSLLYLATFGSFIGFSAGFAMLAKTQFPAVDILKLAFFGPFIGALARSFGGIISDRLGGVRVTLV
NFVLMALFTGLLFLTLPGSGSGSFLAFYVVFMGLFLTAGLGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEAQHEAVTDTA
AALGFISAIGAIGGFFIPKAFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWLVYGRRKSA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=458, Percent_Identity=80.3493449781659, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1787475, Length=459, Percent_Identity=73.202614379085, Blast_Score=662, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49528; Mature: 49397

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQNDKDNHYLLNNWRPENKAFWENKGKAIARRNLWISVTCLLLAFCVWMLFSAVAVNL
CCCCCCCCCCEEECCCCCCCCHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NKVGFHFTTDQLFLLTALPSLSGAILRVPYSFMVPLFGGRYWTVLSTVILIVPCIWLGVA
HHCCEEECCCHHHHHHHHHCCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IQNITTPFWVFIIIALLCGFAGANFASSMGNISFFFPKAKQGSALGVNGGLGNLGVSVMQ
HHHCCHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEECCCCCHHHHHHHHH
MVAPAVIFLPLFTFLGVHGVTQPDGSTITLSNAALVWVPLLLLATVAAWFGMNDIAGSKA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
SIRDQLPVLKRPHMWLLSLLYLATFGSFIGFSAGFAMLAKTQFPAVDILKLAFFGPFIGA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LARSFGGIISDRLGGVRVTLVNFVLMALFTGLLFLTLPGSGSGSFLAFYVVFMGLFLTAG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHC
LGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEAQHEAVTDTAAALGFISAIGAIGGFFIPK
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWLVYGRRKSA
HHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVQNDKDNHYLLNNWRPENKAFWENKGKAIARRNLWISVTCLLLAFCVWMLFSAVAVNL
CCCCCCCCCEEECCCCCCCCHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NKVGFHFTTDQLFLLTALPSLSGAILRVPYSFMVPLFGGRYWTVLSTVILIVPCIWLGVA
HHCCEEECCCHHHHHHHHHCCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IQNITTPFWVFIIIALLCGFAGANFASSMGNISFFFPKAKQGSALGVNGGLGNLGVSVMQ
HHHCCHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEECCCCCHHHHHHHHH
MVAPAVIFLPLFTFLGVHGVTQPDGSTITLSNAALVWVPLLLLATVAAWFGMNDIAGSKA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
SIRDQLPVLKRPHMWLLSLLYLATFGSFIGFSAGFAMLAKTQFPAVDILKLAFFGPFIGA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LARSFGGIISDRLGGVRVTLVNFVLMALFTGLLFLTLPGSGSGSFLAFYVVFMGLFLTAG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHC
LGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEAQHEAVTDTAAALGFISAIGAIGGFFIPK
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWLVYGRRKSA
HHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2233673; 7747940 [H]