Click here to switch to the map view.

The map label for this gene is yfjR [H]

Identifier: 238894732

GI number: 238894732

Start: 2613427

End: 2614302

Strand: Direct

Name: yfjR [H]

Synonym: KP1_2739

Alternate gene names: 238894732

Gene position: 2613427-2614302 (Clockwise)

Preceding gene: 238894731

Following gene: 238894748

Centisome position: 49.79

GC content: 63.7

Gene sequence:

>876_bases
ATGAGTACATTACCAAAGGTCGCGGTGCTGGGACTCGGCGCCATGGGACACGCTTTTGCCAGTAACCTGCTGAAAAACGG
CTTTACCGTTGCCGGCTGGAACCGTTCGCCAGCCCGCGGCGAAGATTTACAGGCGCATGGGTTATCTCTGCACGCCACGC
CGCAGCAGGCGGTGGCCGATGCCGAGGTGATCATCAGTATGCTCGCCGACGGCGAGGCCACCCTCGAGGTACTGGCGCAA
ATCGCGCCCGCCTGTCAGCCGCAGGCGATCTATTGCCAGATGGGCACCATTGGCCTGCCGGAGACCCGGCAGGCCATCGC
GTTGTTGCGGGAACTTCAGCCAGCGATGACCTATATCGATGCCCCGGTGTCGGGGACCAAAGCCCCAGCGGAGAAGGCAC
AGATCCTGGTGCTCGCCAGCGGAGATCGGGAAAAGGGCGCCGCTGCCGAACCGGTGTTTGCGGCTATCTCCCGCGCGACG
CAGTGGTTTGGCGAGGCGGGCAACAGCCAGAAAATGAAGCTGGTCCTCAATGCCTGGCTGATTTCGATGATGCAGGGCAT
CGCGGAGAGCGCCCAGCTGGCGAAGACGCTGGGCTTCACCCCCGACCAGCTGTGGAGCGCCCTGGAAGGCGGCCCGCTGG
CGGCGCCCTATGTCAAAGTGAAACTGGACGCCATCGCCAGCGAGCAGTTTACCCCGCAGATGCAGCTGGCGCATGCCCTG
AAAGATGCCCGGCTGGCGCTTTCCCTGGCCGAGCCGCACACCATGCCGGGGCTGGAAAACATCGCCGAACTGTGGCAGCA
GGCGGCGGACGCGGGATATGCCGGAGAGGATTTGTCTGCGGTATATCAGTGGCTGTCGCCGTCGTCAAAAGCGTAG

Upstream 100 bases:

>100_bases
ACAGCGTCACTTTTCATGCCCGTTATTGCTACTGACATCCGTGCCAGAGATGCGATAGTAGAGGTCCGTTTTTATTAAGA
CGCGAATCAGGACAGACAAG

Downstream 100 bases:

>100_bases
TTTCCTACCCACGCCAGGAAAGAAGTGGCCATGGTGGCCACTTATCCTGGCGGATGGGGCATCAATTCGAATCTATCGTA
CGTTAGCTTAAGCTATCAAT

Product: putative dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSTLPKVAVLGLGAMGHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVADAEVIISMLADGEATLEVLAQ
IAPACQPQAIYCQMGTIGLPETRQAIALLRELQPAMTYIDAPVSGTKAPAEKAQILVLASGDREKGAAAEPVFAAISRAT
QWFGEAGNSQKMKLVLNAWLISMMQGIAESAQLAKTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQFTPQMQLAHAL
KDARLALSLAEPHTMPGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA

Sequences:

>Translated_291_residues
MSTLPKVAVLGLGAMGHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVADAEVIISMLADGEATLEVLAQ
IAPACQPQAIYCQMGTIGLPETRQAIALLRELQPAMTYIDAPVSGTKAPAEKAQILVLASGDREKGAAAEPVFAAISRAT
QWFGEAGNSQKMKLVLNAWLISMMQGIAESAQLAKTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQFTPQMQLAHAL
KDARLALSLAEPHTMPGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA
>Mature_290_residues
STLPKVAVLGLGAMGHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVADAEVIISMLADGEATLEVLAQI
APACQPQAIYCQMGTIGLPETRQAIALLRELQPAMTYIDAPVSGTKAPAEKAQILVLASGDREKGAAAEPVFAAISRATQ
WFGEAGNSQKMKLVLNAWLISMMQGIAESAQLAKTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQFTPQMQLAHALK
DARLALSLAEPHTMPGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=286, Percent_Identity=29.020979020979, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI23308751, Length=297, Percent_Identity=24.9158249158249, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1790315, Length=289, Percent_Identity=28.719723183391, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI145693186, Length=292, Percent_Identity=25.3424657534247, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=26.2237762237762, Blast_Score=75, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28574115, Length=279, Percent_Identity=27.2401433691756, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24655230, Length=297, Percent_Identity=24.9158249158249, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19922568, Length=297, Percent_Identity=24.9158249158249, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30666; Mature: 30535

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLPKVAVLGLGAMGHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVAD
CCCCHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCEEEECCHHHHHHH
AEVIISMLADGEATLEVLAQIAPACQPQAIYCQMGTIGLPETRQAIALLRELQPAMTYID
HHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
APVSGTKAPAEKAQILVLASGDREKGAAAEPVFAAISRATQWFGEAGNSQKMKLVLNAWL
CCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ISMMQGIAESAQLAKTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQFTPQMQLAHAL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHHH
KDARLALSLAEPHTMPGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STLPKVAVLGLGAMGHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVAD
CCCHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCEEEECCHHHHHHH
AEVIISMLADGEATLEVLAQIAPACQPQAIYCQMGTIGLPETRQAIALLRELQPAMTYID
HHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
APVSGTKAPAEKAQILVLASGDREKGAAAEPVFAAISRATQWFGEAGNSQKMKLVLNAWL
CCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ISMMQGIAESAQLAKTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQFTPQMQLAHAL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHHH
KDARLALSLAEPHTMPGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]