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The map label for this gene is vanB [H]

Identifier: 238894700

GI number: 238894700

Start: 2583858

End: 2584847

Strand: Reverse

Name: vanB [H]

Synonym: KP1_2706

Alternate gene names: 238894700

Gene position: 2584847-2583858 (Counterclockwise)

Preceding gene: 238894701

Following gene: 238894698

Centisome position: 49.25

GC content: 67.37

Gene sequence:

>990_bases
ATGAGTGGAGGTCATATGCGCGACATACTCCCGGTGGTGGTCGATGGCCTGTGGCGCCAGGGCGCGAAAAATCTGGCCGT
TCGTCTGGTGAGCGCCGAGGGGCAACCGCTGCCGGCGTGGACGCCCGGCGCCCATATCGATCTCCATCTTCCCTGCGGGC
TGATCCGTCAGTACTCCCTGACCGGCAGCCCGGCCGAACGGGATCGCTATCTGCTCTGCATCGCCCGCGAGTCGCAGTCT
CGCGGTGGCTCCCGTTATATTCATGACACCCTGCGCCCGGGACAGCCATTGATGATCTCTGCCCCGCGCAACCACTTTCC
GCTGCACGAGGGCGGCCATGTGGTGCTTCTCGCCGCCGGGATCGGCATTACGCCGCTGCTGGCGATGGCCCACGCCCGGG
CGGCGTCCGGCGCCAGCTTTACGCTGCACTATTACGTTAGCCGTGCGCAGGAGGCCGCCTTCGCCACCGAGATCGCCCGC
CAACTAACGGGTGGAATCTGCCAGATCCACTGCTCAGACGAGGGGCAGAGTCCGCGCCAGCGGCTGGCGCAGGATCTGGG
GGCTCCGGACGCCGACACCCGGGTCTACTTCTGCGGTCCGCCCGGATTTATGGCCCGGGTTCGCGATACCGCGCGGGCGG
TCGGATGGGAGGAGGCGCAATTGCACAGCGAAGCGTTCCAGCCGCCCGCCCCGACGGCGGCGTCGGCGGCGGACGGAACC
TTCACCATCACCCTCGCCTCCACCGGGGAGCGCTGGCCGGTGCCGGGGGACAAAACCATCGCCCAGGTGCTGCAGGAGCA
TGGCGTCGCGGTGCCGCTCTCCTGCGAGATGGGGATCTGCGGCGCCTGCCTGACGCCGGTACGCGAGGGAACCGTCGACC
ACCGGGATACCGTGCAGTCGGAGGCGGAAAAACAGGCCGCAGAGCAGCATATTGCGCTGTGCTGCTCCCGCAGCCTGTCG
GCCAATTTAGTTATCGATCTCGCGGGATAA

Upstream 100 bases:

>100_bases
CGCTGGATCTGACCCTGGAGGCGCATATTCCGGCGGACCGCAGCTCTATCGCCTACCGGCGCGGGCTGAAGAAGATGGGC
TTTGGCGATTTCTTTCTGGT

Downstream 100 bases:

>100_bases
GCGGCCCGTCACCGGCTGGTCGGCGCGGGCGACCGGTTTCACCCGTACGGCGGCGAAGACAATCACCATCGCGCTCAACA
GCAGCAGACCGCTGCCGGTG

Product: putative oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSGGHMRDILPVVVDGLWRQGAKNLAVRLVSAEGQPLPAWTPGAHIDLHLPCGLIRQYSLTGSPAERDRYLLCIARESQS
RGGSRYIHDTLRPGQPLMISAPRNHFPLHEGGHVVLLAAGIGITPLLAMAHARAASGASFTLHYYVSRAQEAAFATEIAR
QLTGGICQIHCSDEGQSPRQRLAQDLGAPDADTRVYFCGPPGFMARVRDTARAVGWEEAQLHSEAFQPPAPTAASAADGT
FTITLASTGERWPVPGDKTIAQVLQEHGVAVPLSCEMGICGACLTPVREGTVDHRDTVQSEAEKQAAEQHIALCCSRSLS
ANLVIDLAG

Sequences:

>Translated_329_residues
MSGGHMRDILPVVVDGLWRQGAKNLAVRLVSAEGQPLPAWTPGAHIDLHLPCGLIRQYSLTGSPAERDRYLLCIARESQS
RGGSRYIHDTLRPGQPLMISAPRNHFPLHEGGHVVLLAAGIGITPLLAMAHARAASGASFTLHYYVSRAQEAAFATEIAR
QLTGGICQIHCSDEGQSPRQRLAQDLGAPDADTRVYFCGPPGFMARVRDTARAVGWEEAQLHSEAFQPPAPTAASAADGT
FTITLASTGERWPVPGDKTIAQVLQEHGVAVPLSCEMGICGACLTPVREGTVDHRDTVQSEAEKQAAEQHIALCCSRSLS
ANLVIDLAG
>Mature_328_residues
SGGHMRDILPVVVDGLWRQGAKNLAVRLVSAEGQPLPAWTPGAHIDLHLPCGLIRQYSLTGSPAERDRYLLCIARESQSR
GGSRYIHDTLRPGQPLMISAPRNHFPLHEGGHVVLLAAGIGITPLLAMAHARAASGASFTLHYYVSRAQEAAFATEIARQ
LTGGICQIHCSDEGQSPRQRLAQDLGAPDADTRVYFCGPPGFMARVRDTARAVGWEEAQLHSEAFQPPAPTAASAADGTF
TITLASTGERWPVPGDKTIAQVLQEHGVAVPLSCEMGICGACLTPVREGTVDHRDTVQSEAEKQAAEQHIALCCSRSLSA
NLVIDLAG

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=332, Percent_Identity=33.7349397590361, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787098, Length=279, Percent_Identity=25.8064516129032, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787658, Length=340, Percent_Identity=26.1764705882353, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 35097; Mature: 34966

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGHMRDILPVVVDGLWRQGAKNLAVRLVSAEGQPLPAWTPGAHIDLHLPCGLIRQYSL
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHCCC
TGSPAERDRYLLCIARESQSRGGSRYIHDTLRPGQPLMISAPRNHFPLHEGGHVVLLAAG
CCCCCCCCCEEEEEEECCCCCCCCCEEHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECC
IGITPLLAMAHARAASGASFTLHYYVSRAQEAAFATEIARQLTGGICQIHCSDEGQSPRQ
CCHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
RLAQDLGAPDADTRVYFCGPPGFMARVRDTARAVGWEEAQLHSEAFQPPAPTAASAADGT
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCE
FTITLASTGERWPVPGDKTIAQVLQEHGVAVPLSCEMGICGACLTPVREGTVDHRDTVQS
EEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHH
EAEKQAAEQHIALCCSRSLSANLVIDLAG
HHHHHHHHHHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure 
SGGHMRDILPVVVDGLWRQGAKNLAVRLVSAEGQPLPAWTPGAHIDLHLPCGLIRQYSL
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHCCC
TGSPAERDRYLLCIARESQSRGGSRYIHDTLRPGQPLMISAPRNHFPLHEGGHVVLLAAG
CCCCCCCCCEEEEEEECCCCCCCCCEEHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECC
IGITPLLAMAHARAASGASFTLHYYVSRAQEAAFATEIARQLTGGICQIHCSDEGQSPRQ
CCHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
RLAQDLGAPDADTRVYFCGPPGFMARVRDTARAVGWEEAQLHSEAFQPPAPTAASAADGT
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCE
FTITLASTGERWPVPGDKTIAQVLQEHGVAVPLSCEMGICGACLTPVREGTVDHRDTVQS
EEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHH
EAEKQAAEQHIALCCSRSLSANLVIDLAG
HHHHHHHHHHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3170489 [H]