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The map label for this gene is yneF [H]

Identifier: 238894673

GI number: 238894673

Start: 2562666

End: 2564090

Strand: Direct

Name: yneF [H]

Synonym: KP1_2678

Alternate gene names: 238894673

Gene position: 2562666-2564090 (Clockwise)

Preceding gene: 238894672

Following gene: 238894674

Centisome position: 48.83

GC content: 57.12

Gene sequence:

>1425_bases
ATGTCTTCACTGTCGATTCGGTCATTTACGCTGTTTCGTGAAGAACAACCCGTGCGCGATGCATTGGTCTTATTTACGTT
AACGCTACTGCTGCATTTTCTTGGCGCTATGCTGCGTCTGGTGCAGGAGCTCTCTTTCTTCTGGCCGCTCAATGCAGTGA
TGGCCGGGATTTTTGCACGCTATGTATGGCTTAACCGCAGCTATTTTTATGCCGTCTGTTTTGCGGCAATGCTGGTCTAT
GACGGATTAACCTCGCGCTGGGGAATGGGCTTTGCCTCATTGCTGATTAACTTTTCCAATATTGTCTTTATTGTGACGCT
GGCGCAGCTGGTGCTGTGGGATAAACGTCGCGCCGACTCCATGCCGGGCCCGATAAACGCGCTTAATTTATTCTGTTTCT
GCCTGTTAGCCGCCTTACTGTGCGCAGCGGTCGGCGCGCTGGGGTCGGTGGATGTTGAACGCGCCACCTTCGTTCCCCAG
CTGGCGGACTGGTTTAGCGAACAGTTCTCAACGGCGGTATTAATCCTGCCCTTTATTCTGACCCTGACGCTGCCTTCGGC
ATTAAGCGGTTTTCGCTTTCGCCAGCTGCTGCCGGTGCTGGCGCTGGTCCTGTCAATTGCGCTTGGCGTGGCGGTAGGCG
GCGCGGGCAGCATTACCTTCCCGTTACCGGCTTTGATCTGGTGCGCTGTACGCTATCCGCTGCCGCTGACCTGTCTGCTG
ACTTTTCTCACTGGCATCGGCGAAATACTGCTGGTCGCTAATTCGTTAATTCACTTTTCGCCAGATGCGCGAATGCAGCC
GTGGCAGCTGTTCTCCACCCGCCTCGGAATTGCCGCCATGCTGATTAGCCCGGTGATTGTCGCATCCAGTGTCGAAGCGA
TTAACACGCTGGTGAAGCAGCTGGCCCTGCGCGCCGATTTTGACTTTCAGACCCGCGTCTATTCCCGTTCCGGACTGAGC
GAAGCGTTGAAGCGTCAGACGCTGCCGGCGGATAAGCTGCTGACGGTGATGGTGCTGGATATCGATGGCTTCAAACGGGT
GAATGATGCTCTGGGCCATGAAGGCGGCGATTGCGTGCTGACCCAGTTTGCCCAGCAGGTTCGACAGCTGGTGGGTGAGC
AGGGGATGGTGGCCCGTATCGGCGGCGAAGAGTTTGCGGTGGCCGCCGTCGTCGACAGCGCCCAGCAGGGCTATTTGCTG
GCGGAAAAGATCCGTCACGGGGTGGAGTCGCAGCCTTTCGGCCTGGGCCAGAACCCGATCCACCTGACCATCAGTCTGGG
ACTGGAGACGCGCGAGGTAGGCCACGCCCGCATCACCGAGCTGTTTAACCAGCTGCTGTTGGCGGCGGACGATGAGATGG
TGAAAGCCAAACAGAGCGGTCGTAACCAAATTTGTATGCCGACGCTGACCGAAAGCGCGCCATAA

Upstream 100 bases:

>100_bases
GCGCCGGAGTATGCCTGATAAATATTCTCATGTTATGATAATCCGCTAACTAACTATTAACATAATAATTTCCTGAAACA
ATTAGACGGATAATTACTGA

Downstream 100 bases:

>100_bases
GAAAATCCTATCGGGATCCTGCTTGTGCTAATTTTTGGTTAAAAACATAGTGATGTGGTCGATAACAGGGAGCTTTTATG
ACCACATCCTTTTCACTGCG

Product: putative transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSSLSIRSFTLFREEQPVRDALVLFTLTLLLHFLGAMLRLVQELSFFWPLNAVMAGIFARYVWLNRSYFYAVCFAAMLVY
DGLTSRWGMGFASLLINFSNIVFIVTLAQLVLWDKRRADSMPGPINALNLFCFCLLAALLCAAVGALGSVDVERATFVPQ
LADWFSEQFSTAVLILPFILTLTLPSALSGFRFRQLLPVLALVLSIALGVAVGGAGSITFPLPALIWCAVRYPLPLTCLL
TFLTGIGEILLVANSLIHFSPDARMQPWQLFSTRLGIAAMLISPVIVASSVEAINTLVKQLALRADFDFQTRVYSRSGLS
EALKRQTLPADKLLTVMVLDIDGFKRVNDALGHEGGDCVLTQFAQQVRQLVGEQGMVARIGGEEFAVAAVVDSAQQGYLL
AEKIRHGVESQPFGLGQNPIHLTISLGLETREVGHARITELFNQLLLAADDEMVKAKQSGRNQICMPTLTESAP

Sequences:

>Translated_474_residues
MSSLSIRSFTLFREEQPVRDALVLFTLTLLLHFLGAMLRLVQELSFFWPLNAVMAGIFARYVWLNRSYFYAVCFAAMLVY
DGLTSRWGMGFASLLINFSNIVFIVTLAQLVLWDKRRADSMPGPINALNLFCFCLLAALLCAAVGALGSVDVERATFVPQ
LADWFSEQFSTAVLILPFILTLTLPSALSGFRFRQLLPVLALVLSIALGVAVGGAGSITFPLPALIWCAVRYPLPLTCLL
TFLTGIGEILLVANSLIHFSPDARMQPWQLFSTRLGIAAMLISPVIVASSVEAINTLVKQLALRADFDFQTRVYSRSGLS
EALKRQTLPADKLLTVMVLDIDGFKRVNDALGHEGGDCVLTQFAQQVRQLVGEQGMVARIGGEEFAVAAVVDSAQQGYLL
AEKIRHGVESQPFGLGQNPIHLTISLGLETREVGHARITELFNQLLLAADDEMVKAKQSGRNQICMPTLTESAP
>Mature_473_residues
SSLSIRSFTLFREEQPVRDALVLFTLTLLLHFLGAMLRLVQELSFFWPLNAVMAGIFARYVWLNRSYFYAVCFAAMLVYD
GLTSRWGMGFASLLINFSNIVFIVTLAQLVLWDKRRADSMPGPINALNLFCFCLLAALLCAAVGALGSVDVERATFVPQL
ADWFSEQFSTAVLILPFILTLTLPSALSGFRFRQLLPVLALVLSIALGVAVGGAGSITFPLPALIWCAVRYPLPLTCLLT
FLTGIGEILLVANSLIHFSPDARMQPWQLFSTRLGIAAMLISPVIVASSVEAINTLVKQLALRADFDFQTRVYSRSGLSE
ALKRQTLPADKLLTVMVLDIDGFKRVNDALGHEGGDCVLTQFAQQVRQLVGEQGMVARIGGEEFAVAAVVDSAQQGYLLA
EKIRHGVESQPFGLGQNPIHLTISLGLETREVGHARITELFNQLLLAADDEMVKAKQSGRNQICMPTLTESAP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=303, Percent_Identity=54.7854785478548, Blast_Score=334, Evalue=8e-93,
Organism=Escherichia coli, GI1787262, Length=209, Percent_Identity=30.622009569378, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082007, Length=206, Percent_Identity=32.0388349514563, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693134, Length=189, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=31.7365269461078, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=26.9005847953216, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=31.6455696202532, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI87081881, Length=238, Percent_Identity=27.7310924369748, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 51918; Mature: 51787

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEECCCCCCCCC
>Mature Secondary Structure 
SSLSIRSFTLFREEQPVRDALVLFTLTLLLHFLGAMLRLVQELSFFWPLNAVMAGIFAR
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YVWLNRSYFYAVCFAAMLVYDGLTSRWGMGFASLLINFSNIVFIVTLAQLVLWDKRRADS
HHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MPGPINALNLFCFCLLAALLCAAVGALGSVDVERATFVPQLADWFSEQFSTAVLILPFIL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLTLPSALSGFRFRQLLPVLALVLSIALGVAVGGAGSITFPLPALIWCAVRYPLPLTCLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
TFLTGIGEILLVANSLIHFSPDARMQPWQLFSTRLGIAAMLISPVIVASSVEAINTLVKQ
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALRADFDFQTRVYSRSGLSEALKRQTLPADKLLTVMVLDIDGFKRVNDALGHEGGDCVL
HHHHCCCCHHHHHHHHCCHHHHHHHHCCCHHHHHEEEEECCCCHHHHHHHHCCCCCHHHH
TQFAQQVRQLVGEQGMVARIGGEEFAVAAVVDSAQQGYLLAEKIRHGVESQPFGLGQNPI
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCE
HLTISLGLETREVGHARITELFNQLLLAADDEMVKAKQSGRNQICMPTLTESAP
EEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]