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The map label for this gene is fumB [H]

Identifier: 238894564

GI number: 238894564

Start: 2460571

End: 2462223

Strand: Reverse

Name: fumB [H]

Synonym: KP1_2562

Alternate gene names: 238894564

Gene position: 2462223-2460571 (Counterclockwise)

Preceding gene: 238894565

Following gene: 238894563

Centisome position: 46.91

GC content: 59.35

Gene sequence:

>1653_bases
ATGTCGACTAAACCGTTTGTTTATCAGGATCCGTTTCCTCTGGCGCACGACGATACGGAATACTATCTGCTCAGCAAAGA
GCACGTCTCCGTCGCCGAATTTGACGGCCAGCAGGTCCTGAAAGTTGAGCCGCAGGCGCTGACCCTGCTCGCTCAGCAGG
CCTTCCACGACGCCGCCTTTATGCTGCGCGTCTCCCATCAGCAACAGGTCGCCTCGATTTTGCTCGACCCGGAAGCCAGC
GATAACGATAAATATGTCGCCCTGCAGTTCCTGCGCAACTCGGAAATTGCCGCCAAGGGCGTGCTGCCCACCTGCCAGGA
TACCGGGACCGCGATTATCATGGGGAAAAAAGGCCAGCGCGTCTGGACCGGCGGTGGCGATGAAGCCGCCCTGGCGCAGG
GCGTTTACAACACCTATACCGAAGATAACCTGCGCTACTCACAGAACGCGCCGCTGGATATGTATAAAGAGGTTAACACC
GGCACCAACCTGCCGGCGCAGATCGATCTCTATAGCGTCGACGGTGAAGAATATAAGTTCCTGTGCATGGCGAAAGGCGG
CGGTTCCGCCAACAAAACCTATCTGTACCAGGAAACCAAGGCCCTGATCACCCCGGCGAAGCTGAAAAACTACCTCGTTG
AGAAGATGCGCACTCTCGGCACCGCCGCCTGCCCGCCGTACCACATCGCGTTTGTGATCGGCGGCACCTCAGCGGAAGCG
ACGCTGAAGACCGTCAAACTGGCCTCGACCCGCTATTACGACGGTCTGCCGACCGAAGGTAACGAACACGGTCAGGCGTT
CCGCGACGTGCAGCTGGAGCAGGAGTTGCTGCAGGAAGCGCAGAACCTCGGCCTCGGCGCGCAGTTCGGCGGGAAATACT
TCGCCCACGATATCCGGGTGATCCGTCTGCCGCGCCACGGCGCCTCCTGCCCGATCGGCATGGGTGTCTCCTGCTCCGCC
GACCGTAACATCAAAGCGAAAATCAACCGCGACGGTATCTGGATCGAGAAGCTGGAGAACAATCCGGGAAAATATATTCC
TGAGCATCTGCGTCAGGCCGGCGAAGGCGAGGTGGTAAGCATCAACCTGAATCAGCCGATGAGCGAGATCCTGGCGCAGC
TGTCGGCACATCCGGTCTCCACCCGCCTGAGCCTTAACGGCACCATTATCGTGGCCCGCGACATCGCCCACGCCAAGCTG
AAGGAGCTTATCGACAACGGCGAGGCGCTGCCGCAGTACGTCAAAGATCACCCGATCTACTACGCGGGCCCGGCAAAAAC
GCCAGCCGGTTACGCTTCCGGCTCCCTCGGCCCAACCACTGCCGGCCGCATGGACTCTTACGTTGATCTGCTGCAGTCCC
ACGGCGCGAGCATGGTGATGCTGGCGAAAGGCAACCGCAGCCAGCAGGTGACCGATGCCTGCCATAAACACGGCGGCTTC
TACCTTGGAAGCATCGGCGGCCCGGCGGCAGTGCTCGCCCAGCAGAGTATCAAGAGCCTCGAGTGCGTGGCCTATCCGGA
ACTGGGGATGGAAGCTATCTGGAAAATCGAAGTGGAAGATTTTCCGGCGTTTATCCTCGTCGATGATAAAGGCAACGACT
TCTTCCAGCAGATCCAGAATAAGCAGTGCGCGGGCTGTAAACAGCACGGCTAA

Upstream 100 bases:

>100_bases
CGCCCTGCTGCTGATCGGCTATCCGTGGATGTCCATGATGTTCTGATTCATAACCGCCGGGACTACCCCGGCGGTTTTTA
TCTTTATGGAGTAAGTGACT

Downstream 100 bases:

>100_bases
CGCTATCCTCTGACGCTGATATCAGGCCGTCGCCGTTTGGGCGACGGCTTTTTTTATGTCCGCGTTTCCCCAGCTTGAAT
TCACTTCGCACCAGGCTATG

Product: anaerobic class I fumarate hydratase/fumarase B

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSTKPFVYQDPFPLAHDDTEYYLLSKEHVSVAEFDGQQVLKVEPQALTLLAQQAFHDAAFMLRVSHQQQVASILLDPEAS
DNDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIMGKKGQRVWTGGGDEAALAQGVYNTYTEDNLRYSQNAPLDMYKEVNT
GTNLPAQIDLYSVDGEEYKFLCMAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEA
TLKTVKLASTRYYDGLPTEGNEHGQAFRDVQLEQELLQEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPIGMGVSCSA
DRNIKAKINRDGIWIEKLENNPGKYIPEHLRQAGEGEVVSINLNQPMSEILAQLSAHPVSTRLSLNGTIIVARDIAHAKL
KELIDNGEALPQYVKDHPIYYAGPAKTPAGYASGSLGPTTAGRMDSYVDLLQSHGASMVMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQNKQCAGCKQHG

Sequences:

>Translated_550_residues
MSTKPFVYQDPFPLAHDDTEYYLLSKEHVSVAEFDGQQVLKVEPQALTLLAQQAFHDAAFMLRVSHQQQVASILLDPEAS
DNDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIMGKKGQRVWTGGGDEAALAQGVYNTYTEDNLRYSQNAPLDMYKEVNT
GTNLPAQIDLYSVDGEEYKFLCMAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEA
TLKTVKLASTRYYDGLPTEGNEHGQAFRDVQLEQELLQEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPIGMGVSCSA
DRNIKAKINRDGIWIEKLENNPGKYIPEHLRQAGEGEVVSINLNQPMSEILAQLSAHPVSTRLSLNGTIIVARDIAHAKL
KELIDNGEALPQYVKDHPIYYAGPAKTPAGYASGSLGPTTAGRMDSYVDLLQSHGASMVMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQNKQCAGCKQHG
>Mature_549_residues
STKPFVYQDPFPLAHDDTEYYLLSKEHVSVAEFDGQQVLKVEPQALTLLAQQAFHDAAFMLRVSHQQQVASILLDPEASD
NDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIMGKKGQRVWTGGGDEAALAQGVYNTYTEDNLRYSQNAPLDMYKEVNTG
TNLPAQIDLYSVDGEEYKFLCMAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEAT
LKTVKLASTRYYDGLPTEGNEHGQAFRDVQLEQELLQEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPIGMGVSCSAD
RNIKAKINRDGIWIEKLENNPGKYIPEHLRQAGEGEVVSINLNQPMSEILAQLSAHPVSTRLSLNGTIIVARDIAHAKLK
ELIDNGEALPQYVKDHPIYYAGPAKTPAGYASGSLGPTTAGRMDSYVDLLQSHGASMVMLAKGNRSQQVTDACHKHGGFY
LGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQNKQCAGCKQHG

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=87.043795620438, Blast_Score=1013, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=546, Percent_Identity=87.5457875457875, Blast_Score=1008, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=160, Percent_Identity=30, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60052; Mature: 59921

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKPFVYQDPFPLAHDDTEYYLLSKEHVSVAEFDGQQVLKVEPQALTLLAQQAFHDAAF
CCCCCEEEECCCCCCCCCCCEEEEECCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHEE
MLRVSHQQQVASILLDPEASDNDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIMGKKGQR
EEEECHHHHHHHHEECCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALAQGVYNTYTEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYSVDGEEYKF
EEECCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LCMAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEA
EEEECCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCH
TLKTVKLASTRYYDGLPTEGNEHGQAFRDVQLEQELLQEAQNLGLGAQFGGKYFAHDIRV
HEEEEEEECCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPIGMGVSCSADRNIKAKINRDGIWIEKLENNPGKYIPEHLRQAGEGEVVS
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCCCEEE
INLNQPMSEILAQLSAHPVSTRLSLNGTIIVARDIAHAKLKELIDNGEALPQYVKDHPIY
EECCCCHHHHHHHHCCCCCEEEEEECCEEEEEECHHHHHHHHHHCCCCHHHHHHHHCCEE
YAGPAKTPAGYASGSLGPTTAGRMDSYVDLLQSHGASMVMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQN
EEECCCCHHHHHHHHHHHHEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHCC
KQCAGCKQHG
CCCCCCCCCC
>Mature Secondary Structure 
STKPFVYQDPFPLAHDDTEYYLLSKEHVSVAEFDGQQVLKVEPQALTLLAQQAFHDAAF
CCCCEEEECCCCCCCCCCCEEEEECCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHEE
MLRVSHQQQVASILLDPEASDNDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIMGKKGQR
EEEECHHHHHHHHEECCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALAQGVYNTYTEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYSVDGEEYKF
EEECCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LCMAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEA
EEEECCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCH
TLKTVKLASTRYYDGLPTEGNEHGQAFRDVQLEQELLQEAQNLGLGAQFGGKYFAHDIRV
HEEEEEEECCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPIGMGVSCSADRNIKAKINRDGIWIEKLENNPGKYIPEHLRQAGEGEVVS
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCCCEEE
INLNQPMSEILAQLSAHPVSTRLSLNGTIIVARDIAHAKLKELIDNGEALPQYVKDHPIY
EECCCCHHHHHHHHCCCCCEEEEEECCEEEEEECHHHHHHHHHHCCCCHHHHHHHHCCEE
YAGPAKTPAGYASGSLGPTTAGRMDSYVDLLQSHGASMVMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQN
EEECCCCHHHHHHHHHHHHEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHCC
KQCAGCKQHG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]