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The map label for this gene is pcaB [H]

Identifier: 238894560

GI number: 238894560

Start: 2455713

End: 2457065

Strand: Direct

Name: pcaB [H]

Synonym: KP1_2558

Alternate gene names: 238894560

Gene position: 2455713-2457065 (Clockwise)

Preceding gene: 238894559

Following gene: 238894561

Centisome position: 46.79

GC content: 66.74

Gene sequence:

>1353_bases
ATGAGTTTGTTGACCCCGATGCTGCGCAGCAGTCCGCTAACCGACTGGTTCAGCGACGTGCAGCGGGTGCAGGGCATGCT
GGATTTTGAAGCGGCGCTGGCGCAAGCGCAGGCCGCCTGCGGGATGGTGCCGCCGGAAGCGGTCGGGCCAATTGTGGCGG
CCTGCCGCCACGAGGCCATCGATTTTGCCGCGCTGGGCGAGGCGGCGGTGGGAGCCGGTAACCTGGCGATCCCACTGGTG
AAACAGCTGACGGCGCAGGTAAAAGCGCAAGATCCCGAGGCTGCGCGCTACGTTCACTGGGGCGCTACCAGCCAGGACGC
CATCGACACCGGACTGGTTCTCCAGCTGCGCGGGGCGCTGGATGCCGCTGAGACTCTGCTTGAGCAGCTGTTGGCGGCCC
TGGCTCTCCAGGCGGATCGTTATCGCGATACCGTGATGCCGGGACGGACCTGGATGCAGCACGCGCTGCCGGTGACCTTT
GGCCTGAAGCTGGCGGGCACCCTCGACGCGCTCCTGCGCTGGCAGCAGCGACTGCGCGAGATGCGTCCGCGACTGCTGGT
GCTGCAGTTCGGCGGCGCCGCGGGCACTCTGGACGCGCTAAAAGAGAAAGGGCCGGCGGTTGGCCTGGCGCTGGCGCAGA
TCCTCTGTCTGTCGCTGCCGGATACTCCGTGGCACAGCCAGCGCGATCGGCTGCTGGAGGCGGGCGCCTGGTTCGCCGGC
GTCTGCGGCACGCTGGGGAAATTCGCTAACGATTTCTCCCTGCTGATGCAGACCGAAGTGGCTGAGGTCGGCGAGCCGGT
GGCGGAAGGCCGCGGCGGCTCGTCGACGATGCCGCATAAGCGCAATCCGGTGGCCTGCGCGGCGATCCTGACCGCCGCCC
AGCGTACGCCGGGGCTGATGGCGACGCTCTACGCCAGCCAACTCCAGCAGCATGAGCGGGCGCTGGGCGGCTGGCAGGCG
GAGTGGGAGACCCTGCCGGAGCTAATTACCCTGGTGGGCGGGGCGTTGGCGCAAAGCGAGGCGCTGGTGCGCGACATGCA
GGTTTTCCCGCAGAAAATGCGCGCCGATCTCGATATCACCCATGGGCTGATTATGGCTGAGGCGGTGACTCTGGCGCTGG
CGGAATTCATTGGCAAAGCCGAGGCGCATCATCATATCGAGGCGCTGTGCCGCCAGGCGCTGGACCGTCACTGCCCGCTG
GTCGATCTGCTCGCGGCTGACCCACAGGTCAGTCAGTATCTCTCTCGTGAACGCTTAACCACGCTGCTGGACCCGGCAAC
GGCGACCGGCAGCGCCGAACGCTTTGTGCGCCAGGTGCTGGCGCGTTATCAGGAGCAACGTGATGAATCTTGA

Upstream 100 bases:

>100_bases
TCAGCTGAAACGGACCGGCGGTCGTTACGCCCTGTGCACCATGTGCATCGGCGTCGGACAGGGGATCGCCCTGATTATTG
AACGCGTTTAAGGAGCCAGA

Downstream 100 bases:

>100_bases
TTATCAACTCGACGGGCCGGATGGCGCGCCGGTTATCGTCCTCTCCAATTCGCTGGGCACCACCCGGGCGATGTGGCAGC
CGCAGATCGAGGCGTTAACC

Product: putative fumarate lyase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSLLTPMLRSSPLTDWFSDVQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAIDFAALGEAAVGAGNLAIPLV
KQLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGALDAAETLLEQLLAALALQADRYRDTVMPGRTWMQHALPVTF
GLKLAGTLDALLRWQQRLREMRPRLLVLQFGGAAGTLDALKEKGPAVGLALAQILCLSLPDTPWHSQRDRLLEAGAWFAG
VCGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLMATLYASQLQQHERALGGWQA
EWETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDITHGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPL
VDLLAADPQVSQYLSRERLTTLLDPATATGSAERFVRQVLARYQEQRDES

Sequences:

>Translated_450_residues
MSLLTPMLRSSPLTDWFSDVQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAIDFAALGEAAVGAGNLAIPLV
KQLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGALDAAETLLEQLLAALALQADRYRDTVMPGRTWMQHALPVTF
GLKLAGTLDALLRWQQRLREMRPRLLVLQFGGAAGTLDALKEKGPAVGLALAQILCLSLPDTPWHSQRDRLLEAGAWFAG
VCGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLMATLYASQLQQHERALGGWQA
EWETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDITHGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPL
VDLLAADPQVSQYLSRERLTTLLDPATATGSAERFVRQVLARYQEQRDES
>Mature_449_residues
SLLTPMLRSSPLTDWFSDVQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAIDFAALGEAAVGAGNLAIPLVK
QLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGALDAAETLLEQLLAALALQADRYRDTVMPGRTWMQHALPVTFG
LKLAGTLDALLRWQQRLREMRPRLLVLQFGGAAGTLDALKEKGPAVGLALAQILCLSLPDTPWHSQRDRLLEAGAWFAGV
CGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLMATLYASQLQQHERALGGWQAE
WETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDITHGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPLV
DLLAADPQVSQYLSRERLTTLLDPATATGSAERFVRQVLARYQEQRDES

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI31541964, Length=231, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=1e-10,
Organism=Homo sapiens, GI68303542, Length=231, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1790398, Length=232, Percent_Identity=31.0344827586207, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323391, Length=374, Percent_Identity=24.8663101604278, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78710009, Length=343, Percent_Identity=24.4897959183673, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24640179, Length=240, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24640177, Length=240, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 48616; Mature: 48484

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLTPMLRSSPLTDWFSDVQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAI
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DFAALGEAAVGAGNLAIPLVKQLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGAL
HHHHHCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHH
DAAETLLEQLLAALALQADRYRDTVMPGRTWMQHALPVTFGLKLAGTLDALLRWQQRLRE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MRPRLLVLQFGGAAGTLDALKEKGPAVGLALAQILCLSLPDTPWHSQRDRLLEAGAWFAG
HCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHH
VCGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ATLYASQLQQHERALGGWQAEWETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDIT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
HGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPLVDLLAADPQVSQYLSRERLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
TLLDPATATGSAERFVRQVLARYQEQRDES
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLLTPMLRSSPLTDWFSDVQRVQGMLDFEAALAQAQAACGMVPPEAVGPIVAACRHEAI
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DFAALGEAAVGAGNLAIPLVKQLTAQVKAQDPEAARYVHWGATSQDAIDTGLVLQLRGAL
HHHHHCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHH
DAAETLLEQLLAALALQADRYRDTVMPGRTWMQHALPVTFGLKLAGTLDALLRWQQRLRE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MRPRLLVLQFGGAAGTLDALKEKGPAVGLALAQILCLSLPDTPWHSQRDRLLEAGAWFAG
HCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHH
VCGTLGKFANDFSLLMQTEVAEVGEPVAEGRGGSSTMPHKRNPVACAAILTAAQRTPGLM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ATLYASQLQQHERALGGWQAEWETLPELITLVGGALAQSEALVRDMQVFPQKMRADLDIT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
HGLIMAEAVTLALAEFIGKAEAHHHIEALCRQALDRHCPLVDLLAADPQVSQYLSRERLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
TLLDPATATGSAERFVRQVLARYQEQRDES
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]