Click here to switch to the map view.

The map label for this gene is pntA [H]

Identifier: 238894544

GI number: 238894544

Start: 2439565

End: 2441142

Strand: Direct

Name: pntA [H]

Synonym: KP1_2539

Alternate gene names: 238894544

Gene position: 2439565-2441142 (Clockwise)

Preceding gene: 238894537

Following gene: 238894545

Centisome position: 46.48

GC content: 58.17

Gene sequence:

>1578_bases
TTGGGCAGAAAAGTAAACCATCATCAAAAACCGATGGAAGGGAAAACTATGCGTATTGGTGTACCACAAGAAAGGCTAGC
CCAGGAGACCCGGGCCGCCGCCACGCCGAAGACCGTTGAGCAATTGCTCAAACTCGGCTTTAGCGTCGCGGTAGAAAGCG
GCGCCGGGAAGCTGGCCAGCTTTGACGATGAGGCCTTTGCCGAGGCAGGAGCTGAAATCGTGACCGGCGACGAGGTATGG
CAGTCTGATGTCATCCTTAAGGTCAATGCTCCGAATGACGATGAAATTGCCCTGCTGAATCCGGGGACCACGCTGATTAG
CTTTATCTGGCCGGCGCAAAACCCGCAGCTGATGGAAAAACTGGCGGCCCGTAATATCAACGTAATGGCGATGGATTCCG
TGCCGCGTATTTCCCGCGCCCAGTCGCTGGATGCGCTCAGCTCAATGGCCAACATCGCCGGGTACCGCGCTATCGTTGAA
GCGGCGCATGAATTTGGTCGATTCTTTACCGGGCAGATTACGGCGGCAGGCAAGGTTCCGCCCGCCAAGGTGATGGTCAT
TGGCGCCGGGGTAGCCGGGCTGGCGGCGATTGGCGCCGCCAACAGCCTGGGCGCTATTGTTCGCGCCTTCGATACCCGTC
CGGAAGTGAAGGAGCAGGTGCAGAGTATGGGTGCCGAGTTCCTCGAGCTGGACTTTAAAGAGGAAGCGGGCAGCGGCGAT
GGCTACGCCAAGGTGATGTCTGAGGCCTTTATTAAGGCTGAAATGGCGCTGTTCGCCGCGCAGGCGAAAGAGGTCGATAT
CATTGTCACCACGGCGTTAATCCCGGGCAAACCGGCGCCGAAGCTGATCACCCGCGAAATGGTCGACTCCATGAAATCGG
GCAGCGTGGTGGTCGATCTGGCCTCGCAAAACGGCGGCAACTGTGAGTACACCGTACCGGGCGAAGTGGTGACCACCGCC
AACGGCGTGAAAATCATCGGTTACACCGATCTTCCTGGCCGTCTGCCGACCCAGTCCTCCCAGCTCTACGGCACCAACCT
GGTGAACCTGCTGAAGCTGCTGTGCAAAGAGAAAGACGGCAATATCGTTATCGATTTCGATGACGTGGTAGTGCGCGGCG
TGACCGTTGTTCGCGAAGGTGAAATCACCTGGCCGGCGCCGCCGATTCAGGTCTCCGCTCAGCCGCAGGCCGCGGCGAAA
AAAGTGGAAGCGCCGAAAGAAGCGGTGAAACCGGCGTCGCCGTGGCGCAAATATGCGCTGATGGCGCTGGCGATTATTCT
CTTCGGCTGGCTGGCCAACGTGGCGCCAAAAGAGTTCCTCGGCCATTTCACCGTCTTCGCGCTGGCCTGCGTGGTGGGTT
ACTACGTGGTGTGGAACGTCTCCCATGCGCTGCATACGCCGCTGATGTCGGTGACCAATGCCATTTCGGGCATTATCGTG
GTTGGCGCGTTGTTGCAGATAGGCCACGGCGGCTGGGTCAGCTTCCTGAGCTTTATCGCGGTGCTGATCGCCAGCATTAA
TATTTTCGGTGGTTTTACCGTGACTCAGCGCATGCTGAAAATGTTTCGCAAAGGATAA

Upstream 100 bases:

>100_bases
GATCACAATCGGTATTTTCAGTAGGTTATAAAAAGTTTGTTACTGTTTCATTTTGTAGACAGCGAAAGATGGCGAGGAAG
ATTTTGCCGTTCTGGCGTTG

Downstream 100 bases:

>100_bases
GGGGTAGCACATGTCTGGTGGATTAGTAACGGCTGCATACATTGTTGCTGCAATCCTGTTTATTTTCAGCCTGGCGGGCC
TGTCGAAACACGAAACGTCG

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MGRKVNHHQKPMEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLASFDDEAFAEAGAEIVTGDEVW
QSDVILKVNAPNDDEIALLNPGTTLISFIWPAQNPQLMEKLAARNINVMAMDSVPRISRAQSLDALSSMANIAGYRAIVE
AAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGD
GYAKVMSEAFIKAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDLASQNGGNCEYTVPGEVVTTA
NGVKIIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIVIDFDDVVVRGVTVVREGEITWPAPPIQVSAQPQAAAK
KVEAPKEAVKPASPWRKYALMALAIILFGWLANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIV
VGALLQIGHGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG

Sequences:

>Translated_525_residues
MGRKVNHHQKPMEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLASFDDEAFAEAGAEIVTGDEVW
QSDVILKVNAPNDDEIALLNPGTTLISFIWPAQNPQLMEKLAARNINVMAMDSVPRISRAQSLDALSSMANIAGYRAIVE
AAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGD
GYAKVMSEAFIKAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDLASQNGGNCEYTVPGEVVTTA
NGVKIIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIVIDFDDVVVRGVTVVREGEITWPAPPIQVSAQPQAAAK
KVEAPKEAVKPASPWRKYALMALAIILFGWLANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIV
VGALLQIGHGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG
>Mature_524_residues
GRKVNHHQKPMEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLASFDDEAFAEAGAEIVTGDEVWQ
SDVILKVNAPNDDEIALLNPGTTLISFIWPAQNPQLMEKLAARNINVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEA
AHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDG
YAKVMSEAFIKAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDLASQNGGNCEYTVPGEVVTTAN
GVKIIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIVIDFDDVVVRGVTVVREGEITWPAPPIQVSAQPQAAAKK
VEAPKEAVKPASPWRKYALMALAIILFGWLANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVV
GALLQIGHGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=534, Percent_Identity=51.310861423221, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI122939153, Length=534, Percent_Identity=51.310861423221, Blast_Score=484, Evalue=1e-136,
Organism=Escherichia coli, GI1787887, Length=509, Percent_Identity=88.8015717092338, Blast_Score=926, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=535, Percent_Identity=51.0280373831776, Blast_Score=493, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 56001; Mature: 55869

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKVNHHQKPMEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLAS
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCHHEEEECCCCCCCC
FDDEAFAEAGAEIVTGDEVWQSDVILKVNAPNDDEIALLNPGTTLISFIWPAQNPQLMEK
CCHHHHHHCCCEEEECCHHCCCCEEEEEECCCCCCEEEECCCCEEEEEECCCCCHHHHHH
LAARNINVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVP
HHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCC
PAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGD
CCEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCC
GYAKVMSEAFIKAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
ASQNGGNCEYTVPGEVVTTANGVKIIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDG
ECCCCCCEEEECCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
NIVIDFDDVVVRGVTVVREGEITWPAPPIQVSAQPQAAAKKVEAPKEAVKPASPWRKYAL
CEEEEEHHHHHHCEEEEEECCEECCCCCCEECCCCHHHHHHHCCCHHHCCCCCHHHHHHH
MALAIILFGWLANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VGALLQIGHGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GRKVNHHQKPMEGKTMRIGVPQERLAQETRAAATPKTVEQLLKLGFSVAVESGAGKLAS
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCHHEEEECCCCCCCC
FDDEAFAEAGAEIVTGDEVWQSDVILKVNAPNDDEIALLNPGTTLISFIWPAQNPQLMEK
CCHHHHHHCCCEEEECCHHCCCCEEEEEECCCCCCEEEECCCCEEEEEECCCCCHHHHHH
LAARNINVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVP
HHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCC
PAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGD
CCEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCC
GYAKVMSEAFIKAEMALFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKSGSVVVDL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
ASQNGGNCEYTVPGEVVTTANGVKIIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDG
ECCCCCCEEEECCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
NIVIDFDDVVVRGVTVVREGEITWPAPPIQVSAQPQAAAKKVEAPKEAVKPASPWRKYAL
CEEEEEHHHHHHCEEEEEECCEECCCCCCEECCCCHHHHHHHCCCHHHCCCCCHHHHHHH
MALAIILFGWLANVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VGALLQIGHGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]