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The map label for this gene is ldhA [H]

Identifier: 238894468

GI number: 238894468

Start: 2362243

End: 2363232

Strand: Reverse

Name: ldhA [H]

Synonym: KP1_2458

Alternate gene names: 238894468

Gene position: 2363232-2362243 (Counterclockwise)

Preceding gene: 238894469

Following gene: 238894467

Centisome position: 45.03

GC content: 57.88

Gene sequence:

>990_bases
ATGAAAATCGCGGTTTATAGTACGAAGCAGTACGATAAAAAGTACCTGCAGCACGTTAATGATGCATACGGCTTTGAACT
GGAATTCTTCGATTTCCTGCTGACAGCGAAGACTGCCAAAACCGCCAACGGTTGCGAAGCGGTATGTATCTTCGTCAATG
ACGACGGCAGCCGCCCGGTGCTGGAAGAGCTGAAGGCCCACGGGGTGAAATATATCGCCCTGCGCTGCGCCGGGTTTAAC
AACGTCGACCTTGAGGCGGCAAAGGAGCTTGGCCTGCGCGTCGTGCGCGTTCCAGCTTACTCTCCGGAAGCGGTCGCTGA
GCATGCGATCGGTATGATGATGTCGCTCAACCGCCGCATCCACCGCGCTTACCAGCGTACCCGTGATGCCAATTTCTCCC
TCGAAGGCCTCACCGGCTTCACCATGTACGGCAAAACCGCCGGGGTGATCGGCACCGGGAAAATTGGCGTAGCGATGTTG
CGGATCCTTAAAGGCTTCGGCATGCGCCTGCTGGCGTTCGACCCGTACCCAAGCGCCGCCGCGCTGGAGCTGGGGGTAGA
ATATGTTGACCTCGCCACGCTGTACAAGGAATCGGACGTGATCTCCCTGCACTGTCCGCTGACCGACGAAAACTACCACC
TGCTCAATCGCGAAGCCTTCGATCAGATGAAAGACGGGGTGATGGTGATCAACACCAGCCGCGGCGCCCTGATCGACTCC
CAGGCGGCCATCGACGCCCTGAAGCACCAGAAAATTGGCGCGCTTGGGCTGGACGTTTATGAGAACGAACGCGATCTGTT
CTTTGAAGACAAATCCAACGACGTGATCCAGGACGATGTCTTCCGCCGCCTCTCCGCCTGCCATAACGTGCTGTTTACCG
GCCACCAGGCGTTCCTCACCGCCGAGGCGCTGATCAGCATTTCGGAGACCACTCTGGGTAACCTGCAGCAGGTCGCCAAC
GGCGAAACCTGTCCGAACGCCATCGTCTAA

Upstream 100 bases:

>100_bases
AGAATTAGGATTAGCACCCTCTCATTAAACTATTTTTTAATAATTGTATGATTATTTTAAATATGCTACCGTGACGGTAT
AATCACTGGAGAAAAGTCTT

Downstream 100 bases:

>100_bases
TGCCTTTCCCTTTTGTGCTCCTCTCCCGGGGGGAGCACATTCAGATAATCCCCACAGATCCCCGCTGCGATACCGTTACA
CTGGCTTGGTTTTATTAGTT

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKIAVYSTKQYDKKYLQHVNDAYGFELEFFDFLLTAKTAKTANGCEAVCIFVNDDGSRPVLEELKAHGVKYIALRCAGFN
NVDLEAAKELGLRVVRVPAYSPEAVAEHAIGMMMSLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLATLYKESDVISLHCPLTDENYHLLNREAFDQMKDGVMVINTSRGALIDS
QAAIDALKHQKIGALGLDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALISISETTLGNLQQVAN
GETCPNAIV

Sequences:

>Translated_329_residues
MKIAVYSTKQYDKKYLQHVNDAYGFELEFFDFLLTAKTAKTANGCEAVCIFVNDDGSRPVLEELKAHGVKYIALRCAGFN
NVDLEAAKELGLRVVRVPAYSPEAVAEHAIGMMMSLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLATLYKESDVISLHCPLTDENYHLLNREAFDQMKDGVMVINTSRGALIDS
QAAIDALKHQKIGALGLDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALISISETTLGNLQQVAN
GETCPNAIV
>Mature_329_residues
MKIAVYSTKQYDKKYLQHVNDAYGFELEFFDFLLTAKTAKTANGCEAVCIFVNDDGSRPVLEELKAHGVKYIALRCAGFN
NVDLEAAKELGLRVVRVPAYSPEAVAEHAIGMMMSLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLATLYKESDVISLHCPLTDENYHLLNREAFDQMKDGVMVINTSRGALIDS
QAAIDALKHQKIGALGLDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALISISETTLGNLQQVAN
GETCPNAIV

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=274, Percent_Identity=31.7518248175182, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4557497, Length=274, Percent_Identity=31.7518248175182, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI145580578, Length=274, Percent_Identity=31.3868613138686, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI4557499, Length=274, Percent_Identity=31.3868613138686, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI23308577, Length=200, Percent_Identity=34.5, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI145580575, Length=270, Percent_Identity=31.4814814814815, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI6912396, Length=226, Percent_Identity=30.0884955752212, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1787645, Length=329, Percent_Identity=89.3617021276596, Blast_Score=621, Evalue=1e-179,
Organism=Escherichia coli, GI87082289, Length=191, Percent_Identity=33.5078534031414, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1789279, Length=249, Percent_Identity=29.718875502008, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788660, Length=210, Percent_Identity=27.1428571428571, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25147481, Length=274, Percent_Identity=28.4671532846715, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17532191, Length=205, Percent_Identity=27.3170731707317, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=193, Percent_Identity=35.2331606217617, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=232, Percent_Identity=32.7586206896552, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320925, Length=232, Percent_Identity=32.3275862068966, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=27.9411764705882, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6325144, Length=130, Percent_Identity=29.2307692307692, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=32.2222222222222, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=32.2222222222222, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=32.2222222222222, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=32.2222222222222, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI62472511, Length=258, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=28.6307053941909, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28574286, Length=255, Percent_Identity=28.6274509803922, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571528, Length=217, Percent_Identity=29.9539170506912, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45552429, Length=210, Percent_Identity=29.5238095238095, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24585514, Length=210, Percent_Identity=29.5238095238095, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28574282, Length=210, Percent_Identity=29.5238095238095, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28574284, Length=210, Percent_Identity=29.5238095238095, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45551003, Length=210, Percent_Identity=29.5238095238095, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=27.1356783919598, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36364; Mature: 36364

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVYSTKQYDKKYLQHVNDAYGFELEFFDFLLTAKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHH
LEELKAHGVKYIALRCAGFNNVDLEAAKELGLRVVRVPAYSPEAVAEHAIGMMMSLNRRI
HHHHHHCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCEEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLATLYKESDVISLHCPLTDENYHLLNREAFDQMKDGVMVINTSRGALIDS
HHHHCHHHHHHHHHHCCCCEEEEECCCCCCCCEECCHHHHHHHHCCEEEEECCCCCEECH
QAAIDALKHQKIGALGLDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHHHCCCEEECEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALISISETTLGNLQQVANGETCPNAIV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIAVYSTKQYDKKYLQHVNDAYGFELEFFDFLLTAKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHH
LEELKAHGVKYIALRCAGFNNVDLEAAKELGLRVVRVPAYSPEAVAEHAIGMMMSLNRRI
HHHHHHCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCEEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLATLYKESDVISLHCPLTDENYHLLNREAFDQMKDGVMVINTSRGALIDS
HHHHCHHHHHHHHHHCCCCEEEEECCCCCCCCEECCHHHHHHHHCCEEEEECCCCCEECH
QAAIDALKHQKIGALGLDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHHHCCCEEECEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALISISETTLGNLQQVANGETCPNAIV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]