BacMap

Definition	Klebsiella pneumoniae NTUH-K2044 chromosome, complete genome.
Accession	NC_012731
Length	5,248,520

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The map label for this gene is kduD [C]

Identifier: 238894405

GI number: 238894405

Start: 2294914

End: 2295705

Strand: Direct

Name: kduD [C]

Synonym: KP1_2388

Alternate gene names: 238894405

Gene position: 2294914-2295705 (Clockwise)

Preceding gene: 238894403

Following gene: 238894406

Centisome position: 43.72

GC content: 60.23

Gene sequence:

>792_bases
ATGTTTTCAGCAGAGATAAAAGGGGGAAACATGAACAATAGCGCAAAAATCGCCCTGGTGACAGGCGGCAGCCGCGGCCT
GGGGCGCGCAACCGTTGAAGCGCTGGCTCAGCGCGGCGTGAACGTGGTATTGACCTACAACGCGCATTTTGCCGAGGCGA
ACGATGTGGTCGCGCGGGTTGAAGCTCAGGGCTCGCGGGCGATCGCGCTGCCCTTCAGCGCCGGCGAAACCGACACCTTT
GACGGGTTTGTCAGCGCCTTTCAGGAGGCGCTGACGGAGCTGGGGGCCGATCGGTTTCATTACCTGGTTAATAATGCCGG
CAATGCCAGCGGCATGGGGTTTCTGAATGCTACCGAATCGGAGTTCGATGCGCTTTATCGCATCCATGTGAAGGGGGTTT
TTTTCCTCAGCCAAAAATTATTGCCGCTGCTGGCGGATGGCGGGCGAATTGTGAATATCTCGTCCGGACTGACCCGTATC
GTGATGGCGAACCGGGCGCCCTATGCGATCATGAAATCCGCGGTGGAAACCCTGACCCGTTATATGGCGTTTGAGCTGGG
CGGCCGCGGGATCACGGTGAACTGCGTAGCGCCGGGCGCCATCGCCACGGATTTCAGCGGCGGGGTGGTGCGGGATAACC
CGCAGGTCGCTCAGGCGGTAGCGAACATGACGGCGCTGGGCCGACCTGGCCTGCCAGAGGATATCGGGCCGATGATCGCC
TCGCTGTTATCCGATGATCATCGCTGGGTGAATGCCCAGCGCATTGAGGTTTCCGGGGGAATGCGCATCTGA

Upstream 100 bases:

>100_bases
TCAGGGTATCCAGTTTATCCATGGCATTGTTCCAGCTGGCAAAACAATCCTGTCGCGCCATGCGGTCTTATCGTCGGCAT
TCAGAGGCCTATAATCGCTC

Downstream 100 bases:

>100_bases
GCGAAGAAAACCGGGGCTGTTTTCATCCGTAGTCAATCGATATGTCAGTGGATGCCGATATAGCGCGACGCTGTGGAGAG
ATAAGGAAATTATGATCTGC

Product: 7-alpha-hydroxysteroid dehydrogenase

Products: @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate!; NADH. [C]

Alternate protein names: ORFC [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MFSAEIKGGNMNNSAKIALVTGGSRGLGRATVEALAQRGVNVVLTYNAHFAEANDVVARVEAQGSRAIALPFSAGETDTF
DGFVSAFQEALTELGADRFHYLVNNAGNASGMGFLNATESEFDALYRIHVKGVFFLSQKLLPLLADGGRIVNISSGLTRI
VMANRAPYAIMKSAVETLTRYMAFELGGRGITVNCVAPGAIATDFSGGVVRDNPQVAQAVANMTALGRPGLPEDIGPMIA
SLLSDDHRWVNAQRIEVSGGMRI

Sequences:

>Translated_263_residues
MFSAEIKGGNMNNSAKIALVTGGSRGLGRATVEALAQRGVNVVLTYNAHFAEANDVVARVEAQGSRAIALPFSAGETDTF
DGFVSAFQEALTELGADRFHYLVNNAGNASGMGFLNATESEFDALYRIHVKGVFFLSQKLLPLLADGGRIVNISSGLTRI
VMANRAPYAIMKSAVETLTRYMAFELGGRGITVNCVAPGAIATDFSGGVVRDNPQVAQAVANMTALGRPGLPEDIGPMIA
SLLSDDHRWVNAQRIEVSGGMRI
>Mature_263_residues
MFSAEIKGGNMNNSAKIALVTGGSRGLGRATVEALAQRGVNVVLTYNAHFAEANDVVARVEAQGSRAIALPFSAGETDTF
DGFVSAFQEALTELGADRFHYLVNNAGNASGMGFLNATESEFDALYRIHVKGVFFLSQKLLPLLADGGRIVNISSGLTRI
VMANRAPYAIMKSAVETLTRYMAFELGGRGITVNCVAPGAIATDFSGGVVRDNPQVAQAVANMTALGRPGLPEDIGPMIA
SLLSDDHRWVNAQRIEVSGGMRI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=252, Percent_Identity=28.1746031746032, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI5031737, Length=246, Percent_Identity=29.6747967479675, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI15277342, Length=262, Percent_Identity=30.5343511450382, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI7705925, Length=245, Percent_Identity=31.8367346938775, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI33667109, Length=193, Percent_Identity=29.5336787564767, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI19923817, Length=265, Percent_Identity=26.4150943396226, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI126723750, Length=255, Percent_Identity=24.3137254901961, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=33.0645161290323, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI2367175, Length=253, Percent_Identity=32.8063241106719, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788459, Length=252, Percent_Identity=30.1587301587302, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789378, Length=251, Percent_Identity=32.6693227091633, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787335, Length=253, Percent_Identity=32.0158102766798, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787905, Length=263, Percent_Identity=32.6996197718631, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790717, Length=248, Percent_Identity=28.2258064516129, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=30.9236947791165, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=27.734375, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87082160, Length=256, Percent_Identity=28.125, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=266, Percent_Identity=30.0751879699248, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560676, Length=257, Percent_Identity=29.1828793774319, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115534694, Length=262, Percent_Identity=30.1526717557252, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538480, Length=275, Percent_Identity=31.2727272727273, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25147288, Length=252, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=32.0754716981132, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560332, Length=269, Percent_Identity=30.8550185873606, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=252, Percent_Identity=27.3809523809524, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17551412, Length=270, Percent_Identity=29.6296296296296, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=248, Percent_Identity=26.6129032258064, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=260, Percent_Identity=29.6153846153846, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17555706, Length=257, Percent_Identity=30.3501945525292, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562904, Length=274, Percent_Identity=29.9270072992701, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=249, Percent_Identity=26.5060240963855, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=205, Percent_Identity=34.6341463414634, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536651, Length=258, Percent_Identity=29.4573643410853, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=273, Percent_Identity=31.8681318681319, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=30.7984790874525, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=195, Percent_Identity=29.7435897435897, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536025, Length=261, Percent_Identity=27.9693486590038, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17565030, Length=267, Percent_Identity=29.5880149812734, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=200, Percent_Identity=32, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=202, Percent_Identity=30.1980198019802, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=263, Percent_Identity=26.615969581749, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558990, Length=216, Percent_Identity=28.7037037037037, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=202, Percent_Identity=29.7029702970297, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24639444, Length=253, Percent_Identity=33.201581027668, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24643142, Length=251, Percent_Identity=31.0756972111554, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21355319, Length=249, Percent_Identity=28.1124497991968, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI23397609, Length=255, Percent_Identity=29.8039215686275, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24644339, Length=260, Percent_Identity=29.6153846153846, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=32.8125, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28571526, Length=252, Percent_Identity=30.952380952381, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.125 [C]

Molecular weight: Translated: 27781; Mature: 27781

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSAEIKGGNMNNSAKIALVTGGSRGLGRATVEALAQRGVNVVLTYNAHFAEANDVVARV
CCCCEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHEEEE
EAQGSRAIALPFSAGETDTFDGFVSAFQEALTELGADRFHYLVNNAGNASGMGFLNATES
ECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEECCCHH
EFDALYRIHVKGVFFLSQKLLPLLADGGRIVNISSGLTRIVMANRAPYAIMKSAVETLTR
HHHHEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHH
YMAFELGGRGITVNCVAPGAIATDFSGGVVRDNPQVAQAVANMTALGRPGLPEDIGPMIA
HHHHHCCCCCEEEEEECCCCEEECCCCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHH
SLLSDDHRWVNAQRIEVSGGMRI
HHHCCCCCEECEEEEEECCCCCC
>Mature Secondary Structure
MFSAEIKGGNMNNSAKIALVTGGSRGLGRATVEALAQRGVNVVLTYNAHFAEANDVVARV
CCCCEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHEEEE
EAQGSRAIALPFSAGETDTFDGFVSAFQEALTELGADRFHYLVNNAGNASGMGFLNATES
ECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEECCCHH
EFDALYRIHVKGVFFLSQKLLPLLADGGRIVNISSGLTRIVMANRAPYAIMKSAVETLTR
HHHHEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHH
YMAFELGGRGITVNCVAPGAIATDFSGGVVRDNPQVAQAVANMTALGRPGLPEDIGPMIA
HHHHHCCCCCEEEEEECCCCEEECCCCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHH
SLLSDDHRWVNAQRIEVSGGMRI
HHHCCCCCEECEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 15.4 {2-deoxygluconate}} [C]

Substrates: @DEOX03.txt*2-Deoxy-D-Gluconate!; NAD(+) [C]

Specific reaction: @DEOX03.txt*2-Deoxy-D-Gluconate! + NAD(+) = @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate! + NADH. [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]