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The map label for this gene is trpD [H]

Identifier: 238894318

GI number: 238894318

Start: 2208107

End: 2209702

Strand: Reverse

Name: trpD [H]

Synonym: KP1_2297

Alternate gene names: 238894318

Gene position: 2209702-2208107 (Counterclockwise)

Preceding gene: 238894319

Following gene: 238894317

Centisome position: 42.1

GC content: 62.41

Gene sequence:

>1596_bases
ATGGCTGACATTCTGCTGCTCGATAATATCGATTCCTTTACCTATAACCTGGCAGACCAGCTGCGCGCCAACGGCCATAA
CGTAGTGATTTATCGCAACTCCGTTCCGGCGCAGGCGCTGATTGAGCGTCTGGCCACCATGGACAACCCGGTGCTGATGC
TCTCCCCGGGCCCGGGTACGCCAAGCGAAGCCGGCTGCATGCCGGAGCTACTGACCCGTATGCGCGGCAAGCTGCCGATC
ATCGGCATCTGCCTCGGCCACCAGGCCATTGTCGAAGCCTACGGCGGCTATGTCGGCCAGGCGGGGGAGATCCTCCATGG
CAAAGCCTCCAGCATTGAGCACGACGGCCAGGCGATGTTTGCCGGTCTGGCTAACCCGCTGCCGGTGGCGCGCTACCACT
CGCTGGTCGGCAGCAATATTCCCGCCGGGCTGACGATTAACGCCAATTTCAACGGTATGGTGATGGCGGTGCGTCACGAT
TCGGATCGGGTGTGCGGCTTCCAGTTCCACCCGGAATCGATCCTCACCACCCAGGGCGCACGGCTGCTGGAGCAGACCCT
GGCCTGGGCGCTGCAGAAGCTGGAGCATACCAACACCCTCCAGCCGATCCTTGAGAAACTGTACCAGGCGGAACCCCTGA
GCCAGCAGGAGAGCCACCAGCTGTTCTCCGCCGTCGTGCGCGGCGAAGTGAAGCCTGAGCAACTGGCCGCCGCGCTGGTC
AGCATGAAAGTGCGCGGCGAGCAGCCGCAGGAAATTGCCGGCGCCGCTACCGCGCTGCTGGAAAACGCCGCGCCGTTCCC
GCGCCCGGACTACCTGTTTGCCGACATCGTTGGCACCGGTGGCGACGGCAGCAACAGCATCAATATCTCCACCGCCAGCG
CCTTTGTCGCTGCGGCCTGCGGGTTAAAAGTGGCGAAACACGGCAACCGCAGCGTCTCCAGTAAATCAGGCTCTTCTGAC
CTGCTGGCGGCCTTCGGCATTAATCTCGATATGAACGCCGATAAATCCCGCGCCGCGCTTGATGAGCTGGGCGTCTGCTT
CCTGTTCGCGCCGAAGTACCACACCGGTTTCCGCCACGCGATGCCGGTCCGTCAGCAGCTGAAGACCCGCACCCTGTTCA
ACGTGCTGGGGCCGCTGATCAACCCGGCGCATCCGCCGCTGGCGCTGATTGGCGTCTACAGCCCGGAACTGGTGCTGCCG
ATCGCCGAGACCCTGCGCGTGCTGGGTTATCAGCGCGCGGCGGTGGTGCACAGCGGTGGGATGGATGAAGTTTCACTGCA
TGCGCCTACGGTGGTGGCCGAACTGCATAATGGAGAAATTAAAAGCTATCAGCTGACCGCCGACGACTTCGGCCTGACGC
CTTACCATCAGGCGCAGCTGGCGGGCGGCACCCCGGAAGAAAACCGTGACATTCTGACGCGCTTGCTACAAGGTAAAGGT
GAAGCCGCTCACGAAGCCGCCGTCGCCGCCAACGTCGCCATGTTGATGCGTTTACACGGGCATGAAGATCTGAAAGCTAA
CGCTCAGCAGGTACTGGATGTGCTGCACAGCGGCGCGGCCTATGACAGAGTGACCGCCTTAGCGGCAAGAGGGTAA

Upstream 100 bases:

>100_bases
TCGACTCGGTTCCCCAGTCGGAAGCGGATGAGACCCGAAATAAAGCCCGCGCGGTGCTGCGCGCCATTGCCCAGGCCCAT
CACGCGAAGGAGATTTTCTG

Downstream 100 bases:

>100_bases
AGAATGCAAACCGTTTTAGCAAAAATCGTTGCCGACAAAGCGATTTGGGTGGAAGCCCGCAAACAACAACAACCGTTAGC
CAGTTTTCAAAATGAGATCG

Product: bifunctional glutamine amidotransferase/anthranilate phosphoribosyltransferase

Products: NA

Alternate protein names: Glutamine amidotransferase; Anthranilate phosphoribosyltransferase [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MADILLLDNIDSFTYNLADQLRANGHNVVIYRNSVPAQALIERLATMDNPVLMLSPGPGTPSEAGCMPELLTRMRGKLPI
IGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMFAGLANPLPVARYHSLVGSNIPAGLTINANFNGMVMAVRHD
SDRVCGFQFHPESILTTQGARLLEQTLAWALQKLEHTNTLQPILEKLYQAEPLSQQESHQLFSAVVRGEVKPEQLAAALV
SMKVRGEQPQEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSD
LLAAFGINLDMNADKSRAALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLP
IAETLRVLGYQRAAVVHSGGMDEVSLHAPTVVAELHNGEIKSYQLTADDFGLTPYHQAQLAGGTPEENRDILTRLLQGKG
EAAHEAAVAANVAMLMRLHGHEDLKANAQQVLDVLHSGAAYDRVTALAARG

Sequences:

>Translated_531_residues
MADILLLDNIDSFTYNLADQLRANGHNVVIYRNSVPAQALIERLATMDNPVLMLSPGPGTPSEAGCMPELLTRMRGKLPI
IGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMFAGLANPLPVARYHSLVGSNIPAGLTINANFNGMVMAVRHD
SDRVCGFQFHPESILTTQGARLLEQTLAWALQKLEHTNTLQPILEKLYQAEPLSQQESHQLFSAVVRGEVKPEQLAAALV
SMKVRGEQPQEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSD
LLAAFGINLDMNADKSRAALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLP
IAETLRVLGYQRAAVVHSGGMDEVSLHAPTVVAELHNGEIKSYQLTADDFGLTPYHQAQLAGGTPEENRDILTRLLQGKG
EAAHEAAVAANVAMLMRLHGHEDLKANAQQVLDVLHSGAAYDRVTALAARG
>Mature_530_residues
ADILLLDNIDSFTYNLADQLRANGHNVVIYRNSVPAQALIERLATMDNPVLMLSPGPGTPSEAGCMPELLTRMRGKLPII
GICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMFAGLANPLPVARYHSLVGSNIPAGLTINANFNGMVMAVRHDS
DRVCGFQFHPESILTTQGARLLEQTLAWALQKLEHTNTLQPILEKLYQAEPLSQQESHQLFSAVVRGEVKPEQLAAALVS
MKVRGEQPQEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDL
LAAFGINLDMNADKSRAALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPI
AETLRVLGYQRAAVVHSGGMDEVSLHAPTVVAELHNGEIKSYQLTADDFGLTPYHQAQLAGGTPEENRDILTRLLQGKGE
AAHEAAVAANVAMLMRLHGHEDLKANAQQVLDVLHSGAAYDRVTALAARG

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0547

COG function: function code E; Anthranilate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=531, Percent_Identity=94.3502824858757, Blast_Score=985, Evalue=0.0,
Organism=Escherichia coli, GI1789760, Length=187, Percent_Identity=44.9197860962567, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322638, Length=227, Percent_Identity=36.5638766519824, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6320561, Length=323, Percent_Identity=32.5077399380805, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324361, Length=208, Percent_Identity=27.8846153846154, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005940
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR000312
- InterPro:   IPR017459
- InterPro:   IPR020072
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase; PF02885 Glycos_trans_3N; PF00591 Glycos_transf_3 [H]

EC number: =4.1.3.27; =2.4.2.18 [H]

Molecular weight: Translated: 56648; Mature: 56517

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADILLLDNIDSFTYNLADQLRANGHNVVIYRNSVPAQALIERLATMDNPVLMLSPGPGT
CCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCC
PSEAGCMPELLTRMRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF
CCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCHHHH
AGLANPLPVARYHSLVGSNIPAGLTINANFNGMVMAVRHDSDRVCGFQFHPESILTTQGA
HHCCCCCCHHHHHHHHCCCCCCCEEEECCCCEEEEEEEECCCCEEEEEECCHHHHHHHHH
RLLEQTLAWALQKLEHTNTLQPILEKLYQAEPLSQQESHQLFSAVVRGEVKPEQLAAALV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
SMKVRGEQPQEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAAC
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHH
GLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRAALDELGVCFLFAPKYHTGFRHA
HHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHH
MPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPIAETLRVLGYQRAAVVHSGG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHEEEECCC
MDEVSLHAPTVVAELHNGEIKSYQLTADDFGLTPYHQAQLAGGTPEENRDILTRLLQGKG
CCCEEECCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCC
EAAHEAAVAANVAMLMRLHGHEDLKANAQQVLDVLHSGAAYDRVTALAARG
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ADILLLDNIDSFTYNLADQLRANGHNVVIYRNSVPAQALIERLATMDNPVLMLSPGPGT
CCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCC
PSEAGCMPELLTRMRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF
CCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCHHHH
AGLANPLPVARYHSLVGSNIPAGLTINANFNGMVMAVRHDSDRVCGFQFHPESILTTQGA
HHCCCCCCHHHHHHHHCCCCCCCEEEECCCCEEEEEEEECCCCEEEEEECCHHHHHHHHH
RLLEQTLAWALQKLEHTNTLQPILEKLYQAEPLSQQESHQLFSAVVRGEVKPEQLAAALV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
SMKVRGEQPQEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAAC
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHH
GLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRAALDELGVCFLFAPKYHTGFRHA
HHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHH
MPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPIAETLRVLGYQRAAVVHSGG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHEEEECCC
MDEVSLHAPTVVAELHNGEIKSYQLTADDFGLTPYHQAQLAGGTPEENRDILTRLLQGKG
CCCEEECCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCC
EAAHEAAVAANVAMLMRLHGHEDLKANAQQVLDVLHSGAAYDRVTALAARG
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7038627; 6283099; 9097039; 9278503; 4594441 [H]