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The map label for this gene is rusA [H]

Identifier: 238894203

GI number: 238894203

Start: 2105115

End: 2105513

Strand: Direct

Name: rusA [H]

Synonym: KP1_2168

Alternate gene names: 238894203

Gene position: 2105115-2105513 (Clockwise)

Preceding gene: 238894202

Following gene: 238894204

Centisome position: 40.11

GC content: 51.13

Gene sequence:

>399_bases
ATGAAATTTATTCTTCCTTTCCCGCCCAGCGTGAACTCCTACTGGCGGTCCCCAAATAAGGGGACTGCAAAAGGTAAATT
GCTGGTCAGCGAAGCCGGCCGCAAATTCAAACATGCTGTAAGAGCAGCGATTATCGAACAGCTGAAAGCAGTCCCAAAAC
CCTCCGCTTCACCAGCGGAGGTAGTCATTGTCCTGCATCCGCCTGATTACCGCCGCCGCGATCTGGACAACTACAACAAG
GCGCTTTTCGACGCACTTACATACGCCGGTATCTGGGAGGATGACAGCCAGGTAAAGAGAATGACGATCGAGTGGGGTGG
GAATGCAAAGGGAGGGAGAGTTGAGATCACCATAACGGCATTCAATAAAGTGCTGGATGTTTGTTCAGTGGTAGGTTGA

Upstream 100 bases:

>100_bases
TTACGAAAATGACGCTATGGAGCCTCATGTCCGAACTGGGGCAGCTATGTTATTGCGGCGGGGAAGTTCCTTTCGAACTT
AAAATTTCCCTGGAGGATGA

Downstream 100 bases:

>100_bases
AGGCTATGCAATCAGGCATTAATCTCAAGGTGTGTAAACGAACCGGGCGTGCAGGCCCGATCGTCACGTTAAAGTGTATG
GAGATAAATATGGCTAACCA

Product: bacteriophage V crossover junction endodeoxyribonuclease

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MKFILPFPPSVNSYWRSPNKGTAKGKLLVSEAGRKFKHAVRAAIIEQLKAVPKPSASPAEVVIVLHPPDYRRRDLDNYNK
ALFDALTYAGIWEDDSQVKRMTIEWGGNAKGGRVEITITAFNKVLDVCSVVG

Sequences:

>Translated_132_residues
MKFILPFPPSVNSYWRSPNKGTAKGKLLVSEAGRKFKHAVRAAIIEQLKAVPKPSASPAEVVIVLHPPDYRRRDLDNYNK
ALFDALTYAGIWEDDSQVKRMTIEWGGNAKGGRVEITITAFNKVLDVCSVVG
>Mature_132_residues
MKFILPFPPSVNSYWRSPNKGTAKGKLLVSEAGRKFKHAVRAAIIEQLKAVPKPSASPAEVVIVLHPPDYRRRDLDNYNK
ALFDALTYAGIWEDDSQVKRMTIEWGGNAKGGRVEITITAFNKVLDVCSVVG

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=117, Percent_Identity=41.8803418803419, Blast_Score=82, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 14640; Mature: 14640

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFILPFPPSVNSYWRSPNKGTAKGKLLVSEAGRKFKHAVRAAIIEQLKAVPKPSASPAE
CCEECCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VVIVLHPPDYRRRDLDNYNKALFDALTYAGIWEDDSQVKRMTIEWGGNAKGGRVEITITA
EEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEEHH
FNKVLDVCSVVG
HHHHHHHHHHCC
>Mature Secondary Structure
MKFILPFPPSVNSYWRSPNKGTAKGKLLVSEAGRKFKHAVRAAIIEQLKAVPKPSASPAE
CCEECCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VVIVLHPPDYRRRDLDNYNKALFDALTYAGIWEDDSQVKRMTIEWGGNAKGGRVEITITA
EEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEEHH
FNKVLDVCSVVG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA