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The map label for this gene is alkK [H]

Identifier: 238894165

GI number: 238894165

Start: 2067570

End: 2069294

Strand: Direct

Name: alkK [H]

Synonym: KP1_2124

Alternate gene names: 238894165

Gene position: 2067570-2069294 (Clockwise)

Preceding gene: 238894164

Following gene: 238894166

Centisome position: 39.39

GC content: 63.71

Gene sequence:

>1725_bases
GTGCAGACCGGCAAATGTAGACCAGATTCAATAGGCGCCCGCACGATCGCGCCGCGCCCCTCGATTCCGCCGGACGTCTG
TAAAGGAGACCCCGCCATGCCGTCACCGATGATTTATCAGGATCTCACCACTGCCGCGCTGCTCGGCCATGCGGCGCAGT
ATCACAGCGAGACCGAGATTGTCTCGGTGAGTACTGGCGGTGAGAAGGAGCGCAGCTGCTGGGGGGAGGTGGCCAGCCGT
GCTCAGCGGCTGGCATCGGCGCTGGCGTCGCTGGGGCTGCCGCCGGGCGCCCGCTGCGCCACGCTGGCATGGAACAACCG
CCGCCATCTGGAGATCTATTTTGCCGTCGCCTCCGGCGGCTGGGTGACGCACACCGTCAATCCGCGGCTGTCGGTTGACC
ATCTGCGGTATATCCTCAACGACGCTGCCGATGAAGTGCTGTTTTTTGACCAGACCTTTTTGCCGCTGGTAGCGCAGCTG
CTGCCGCAGCTGCCGACGGTGAAACACGTCGTGCTGATGGAGTCCCGCAGCGAAGCGGCGCTCAGCCAGCTGCCCTCATT
GCTGTTTTATGACGATCTGTTGCAGCAGGGGATGGCAGACTATCGCTGGCCGCAGCTCAATGAACTGACTCCGGCTTCGC
TCTGTTACACCTCGGGAACCACCGGCCGGCCGAAAGGGGTATTGAACACCCATCGTTCGCTGGTCCTGCACGCCCTGAGC
GGCAACCAGCCGGACGCCGCCGGCATATCGGCGAAGGACAGCCTGTTGCCGGTGGTGCCGATGTTCCATGTCAACGCGTG
GGGGACGCCGTTTATCGCCGCCATGGTCGGCGCGCGGCTGGTGCTGCCGGGGCCGCACCTCGATGGCGACAGCCTGCTGC
AGCTGCTGGCCGCCGAGAAGGTGACGGTGGGGTTTGGCGTGCCGGTGATCTGGGCCGGCTTGCTGGCGGCGATGCGCCGG
ACAGAGGTCCGGCTGCCTGAATTTAAACGTGCGCTGGTCGGCGGCTCCGCGCTGCCGCCGTCGATGGCCGAGGCCTTTCA
GCGTGATTACGGCATTGCGCTGACCCACGCCTGGGGAATGACCGAAACATCGCCCATCGGCACCATCAACACACCGTTGA
GCAAACATGACGCGCTCCCGGCACAGGAGCAGCAAAAGCAGCGCGCCGGTCAGGGGCGGCCCATTTTCGGCATCGAACTG
CAGGTGGTGGACGTGGATGGCGAACCGTTGCCCCGGGATGGTCAAAGCCAGGGTTATCTGCAGGTCAGAGGGCACTGGGT
GGTAGAGCAATACTATGGTCAGGACGCGAGCGCCCTGACCGCGGCAGGCTGGTTTGATACCGGTGATATCGGTACGCTCG
ACGCCAATGGTTATCTGGTGATCAGCGATCGGGCAAAGGATATTATCAAGTCCGGTGGCGAGTGGATCTCCACCGTTGAG
CTGGAGAATATCGCCATCGCGCATCCGGGGGTGCGCAGCGCGGCGGCCATCGCCGCCCGCCATCCGCGCTGGGACGAGCG
GCCGGTGCTGCTGTGCGTCCGTGCGGAAGGGGGCGAAGTGGAGGAGACCGATCTGCTGAGCTGGTTTGAGAAGCGGGTGC
CTAAATGGCAGATCCCGGACCGGGTGATATTTGTCGATGCGCTGCCGGTCAGCGCCACGGGTAAGGTGCTCAAAAATCAG
CTGCGCCAGGCCTACGGAGAGATTTTAATGAGTGAGGGAAAATGA

Upstream 100 bases:

>100_bases
TCTCTGAGTAATGATCTTCCGGTCATGAGTTTGTCTTTTCCTTTCTGAACCATTTCCCACTCTATTTTGTGCGTGACCGC
TGATTTCTGTACGTTTTTTT

Downstream 100 bases:

>100_bases
GCCATGCGCGACCCGGTCTGACGACCTGTTCTCAGTATGATGCGGCCCTGCACGCCTTGTCGGCGGCGCGCCAGCGCTGG
GCGGAAACCTCAGTGAACCG

Product: crotonobetaine/carnitine-CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MQTGKCRPDSIGARTIAPRPSIPPDVCKGDPAMPSPMIYQDLTTAALLGHAAQYHSETEIVSVSTGGEKERSCWGEVASR
AQRLASALASLGLPPGARCATLAWNNRRHLEIYFAVASGGWVTHTVNPRLSVDHLRYILNDAADEVLFFDQTFLPLVAQL
LPQLPTVKHVVLMESRSEAALSQLPSLLFYDDLLQQGMADYRWPQLNELTPASLCYTSGTTGRPKGVLNTHRSLVLHALS
GNQPDAAGISAKDSLLPVVPMFHVNAWGTPFIAAMVGARLVLPGPHLDGDSLLQLLAAEKVTVGFGVPVIWAGLLAAMRR
TEVRLPEFKRALVGGSALPPSMAEAFQRDYGIALTHAWGMTETSPIGTINTPLSKHDALPAQEQQKQRAGQGRPIFGIEL
QVVDVDGEPLPRDGQSQGYLQVRGHWVVEQYYGQDASALTAAGWFDTGDIGTLDANGYLVISDRAKDIIKSGGEWISTVE
LENIAIAHPGVRSAAAIAARHPRWDERPVLLCVRAEGGEVEETDLLSWFEKRVPKWQIPDRVIFVDALPVSATGKVLKNQ
LRQAYGEILMSEGK

Sequences:

>Translated_574_residues
MQTGKCRPDSIGARTIAPRPSIPPDVCKGDPAMPSPMIYQDLTTAALLGHAAQYHSETEIVSVSTGGEKERSCWGEVASR
AQRLASALASLGLPPGARCATLAWNNRRHLEIYFAVASGGWVTHTVNPRLSVDHLRYILNDAADEVLFFDQTFLPLVAQL
LPQLPTVKHVVLMESRSEAALSQLPSLLFYDDLLQQGMADYRWPQLNELTPASLCYTSGTTGRPKGVLNTHRSLVLHALS
GNQPDAAGISAKDSLLPVVPMFHVNAWGTPFIAAMVGARLVLPGPHLDGDSLLQLLAAEKVTVGFGVPVIWAGLLAAMRR
TEVRLPEFKRALVGGSALPPSMAEAFQRDYGIALTHAWGMTETSPIGTINTPLSKHDALPAQEQQKQRAGQGRPIFGIEL
QVVDVDGEPLPRDGQSQGYLQVRGHWVVEQYYGQDASALTAAGWFDTGDIGTLDANGYLVISDRAKDIIKSGGEWISTVE
LENIAIAHPGVRSAAAIAARHPRWDERPVLLCVRAEGGEVEETDLLSWFEKRVPKWQIPDRVIFVDALPVSATGKVLKNQ
LRQAYGEILMSEGK
>Mature_574_residues
MQTGKCRPDSIGARTIAPRPSIPPDVCKGDPAMPSPMIYQDLTTAALLGHAAQYHSETEIVSVSTGGEKERSCWGEVASR
AQRLASALASLGLPPGARCATLAWNNRRHLEIYFAVASGGWVTHTVNPRLSVDHLRYILNDAADEVLFFDQTFLPLVAQL
LPQLPTVKHVVLMESRSEAALSQLPSLLFYDDLLQQGMADYRWPQLNELTPASLCYTSGTTGRPKGVLNTHRSLVLHALS
GNQPDAAGISAKDSLLPVVPMFHVNAWGTPFIAAMVGARLVLPGPHLDGDSLLQLLAAEKVTVGFGVPVIWAGLLAAMRR
TEVRLPEFKRALVGGSALPPSMAEAFQRDYGIALTHAWGMTETSPIGTINTPLSKHDALPAQEQQKQRAGQGRPIFGIEL
QVVDVDGEPLPRDGQSQGYLQVRGHWVVEQYYGQDASALTAAGWFDTGDIGTLDANGYLVISDRAKDIIKSGGEWISTVE
LENIAIAHPGVRSAAAIAARHPRWDERPVLLCVRAEGGEVEETDLLSWFEKRVPKWQIPDRVIFVDALPVSATGKVLKNQ
LRQAYGEILMSEGK

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=529, Percent_Identity=25.1417769376182, Blast_Score=162, Evalue=1e-39,
Organism=Homo sapiens, GI187761345, Length=534, Percent_Identity=25.0936329588015, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI187761343, Length=534, Percent_Identity=25.0936329588015, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI38505220, Length=517, Percent_Identity=24.1779497098646, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI42544132, Length=516, Percent_Identity=21.8992248062016, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI115511026, Length=461, Percent_Identity=24.295010845987, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI122937307, Length=515, Percent_Identity=22.9126213592233, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311624, Length=503, Percent_Identity=22.0675944333996, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI157311622, Length=503, Percent_Identity=22.0675944333996, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI28416953, Length=564, Percent_Identity=24.290780141844, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI58082049, Length=507, Percent_Identity=20.7100591715976, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI13375727, Length=407, Percent_Identity=24.5700245700246, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI221142682, Length=531, Percent_Identity=25.4237288135593, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1788107, Length=573, Percent_Identity=25.130890052356, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI145693145, Length=365, Percent_Identity=30.1369863013699, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1786810, Length=547, Percent_Identity=26.8738574040219, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1790505, Length=440, Percent_Identity=23.8636363636364, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789201, Length=357, Percent_Identity=24.6498599439776, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788595, Length=476, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786801, Length=333, Percent_Identity=27.3273273273273, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994703, Length=531, Percent_Identity=25.2354048964218, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71994694, Length=531, Percent_Identity=25.2354048964218, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71994690, Length=531, Percent_Identity=25.2354048964218, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17558820, Length=538, Percent_Identity=25.4646840148699, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI32563687, Length=510, Percent_Identity=25.4901960784314, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17557194, Length=536, Percent_Identity=24.0671641791045, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=541, Percent_Identity=23.4750462107209, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=536, Percent_Identity=21.6417910447761, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=531, Percent_Identity=24.4821092278719, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=509, Percent_Identity=22.9862475442043, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=464, Percent_Identity=23.2758620689655, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=367, Percent_Identity=24.5231607629428, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=499, Percent_Identity=21.2424849699399, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17510401, Length=217, Percent_Identity=27.1889400921659, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17507435, Length=549, Percent_Identity=21.4936247723133, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=371, Percent_Identity=25.8760107816712, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24581924, Length=602, Percent_Identity=26.4119601328904, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21355181, Length=488, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18859661, Length=362, Percent_Identity=25.9668508287293, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648253, Length=526, Percent_Identity=22.2433460076046, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648255, Length=526, Percent_Identity=22.4334600760456, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648260, Length=513, Percent_Identity=24.9512670565302, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356947, Length=506, Percent_Identity=24.1106719367589, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24653035, Length=494, Percent_Identity=22.4696356275304, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI161076582, Length=502, Percent_Identity=22.5099601593625, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24667959, Length=384, Percent_Identity=25.2604166666667, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI62472339, Length=384, Percent_Identity=25.2604166666667, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24667955, Length=384, Percent_Identity=25.2604166666667, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20130357, Length=367, Percent_Identity=25.3405994550409, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356441, Length=487, Percent_Identity=21.3552361396304, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358303, Length=532, Percent_Identity=20.6766917293233, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648257, Length=398, Percent_Identity=24.6231155778894, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281365686, Length=258, Percent_Identity=24.8062015503876, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 62286; Mature: 62286

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTGKCRPDSIGARTIAPRPSIPPDVCKGDPAMPSPMIYQDLTTAALLGHAAQYHSETEI
CCCCCCCCCCCCCCEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
VSVSTGGEKERSCWGEVASRAQRLASALASLGLPPGARCATLAWNNRRHLEIYFAVASGG
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEECCC
WVTHTVNPRLSVDHLRYILNDAADEVLFFDQTFLPLVAQLLPQLPTVKHVVLMESRSEAA
EEEEECCCCCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCHHH
LSQLPSLLFYDDLLQQGMADYRWPQLNELTPASLCYTSGTTGRPKGVLNTHRSLVLHALS
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCCCCCCCHHHHHHHHEEEECC
GNQPDAAGISAKDSLLPVVPMFHVNAWGTPFIAAMVGARLVLPGPHLDGDSLLQLLAAEK
CCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHHH
VTVGFGVPVIWAGLLAAMRRTEVRLPEFKRALVGGSALPPSMAEAFQRDYGIALTHAWGM
EEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCC
TETSPIGTINTPLSKHDALPAQEQQKQRAGQGRPIFGIELQVVDVDGEPLPRDGQSQGYL
CCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEE
QVRGHWVVEQYYGQDASALTAAGWFDTGDIGTLDANGYLVISDRAKDIIKSGGEWISTVE
EEECEEEEEHHHCCCCCCEEECCCCCCCCCEEECCCCEEEECCHHHHHHHCCCCEEEEEE
LENIAIAHPGVRSAAAIAARHPRWDERPVLLCVRAEGGEVEETDLLSWFEKRVPKWQIPD
ECCEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCC
RVIFVDALPVSATGKVLKNQLRQAYGEILMSEGK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQTGKCRPDSIGARTIAPRPSIPPDVCKGDPAMPSPMIYQDLTTAALLGHAAQYHSETEI
CCCCCCCCCCCCCCEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
VSVSTGGEKERSCWGEVASRAQRLASALASLGLPPGARCATLAWNNRRHLEIYFAVASGG
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEECCC
WVTHTVNPRLSVDHLRYILNDAADEVLFFDQTFLPLVAQLLPQLPTVKHVVLMESRSEAA
EEEEECCCCCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCHHH
LSQLPSLLFYDDLLQQGMADYRWPQLNELTPASLCYTSGTTGRPKGVLNTHRSLVLHALS
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCCCCCCCHHHHHHHHEEEECC
GNQPDAAGISAKDSLLPVVPMFHVNAWGTPFIAAMVGARLVLPGPHLDGDSLLQLLAAEK
CCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHHH
VTVGFGVPVIWAGLLAAMRRTEVRLPEFKRALVGGSALPPSMAEAFQRDYGIALTHAWGM
EEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCC
TETSPIGTINTPLSKHDALPAQEQQKQRAGQGRPIFGIELQVVDVDGEPLPRDGQSQGYL
CCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEE
QVRGHWVVEQYYGQDASALTAAGWFDTGDIGTLDANGYLVISDRAKDIIKSGGEWISTVE
EEECEEEEEHHHCCCCCCEEECCCCCCCCCEEECCCCEEEECCHHHHHHHCCCCEEEEEE
LENIAIAHPGVRSAAAIAARHPRWDERPVLLCVRAEGGEVEETDLLSWFEKRVPKWQIPD
ECCEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCC
RVIFVDALPVSATGKVLKNQLRQAYGEILMSEGK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]