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The map label for this gene is srlE [H]

Identifier: 238894159

GI number: 238894159

Start: 2060728

End: 2062446

Strand: Direct

Name: srlE [H]

Synonym: KP1_2116

Alternate gene names: 238894159

Gene position: 2060728-2062446 (Clockwise)

Preceding gene: 238894158

Following gene: 238894160

Centisome position: 39.26

GC content: 58.46

Gene sequence:

>1719_bases
ATGGAAAAACATCTGTTGATTAGCAAGGGCTGTAAGGGCTGGGGCGGGCCGCTGACTATCACGCCAGGTCAGGGGAAAAA
GATTGCGTACATCACCGGGGGGATCCGTCCCCCGGTCGTCGACCGCCTGAGCGAACTGACCGGCTGGCCTTCCATTGATG
TATTCAAGAATGGCGAACCGCCGGCGGAAGAGATTGGTTTAATGGTCATCGACTGCGGCGGCACGTTGCGCTGCGGACTC
TATCCGAAGCGGGGAATTCCCACCATTAACCTGCATCCCACCGGTAAAAGCGGCCCGCTGGCGGAATTTATACACGAAGG
GATCTACGTCTCCGGCGTGACGCCAGCCTGCATTGAGATGGTCTGGCCGCAAGGGGAAGGCGGAAAGCTTGGGATCGTGG
CTGACGATCTGACCGGGGCAACTACCGTCGGGGTGCTGCTGGCGCGCAGTGGTTTAAAAACCGCCGCGTTTTTCGATACC
GAATCCTTCGCCCGCAATGAGGTAGAGTATCCGGCGATGGTGGTCAGCAGCGACAGCCGCCCATTGCCGAAAGCGGAGGC
CCAGCACAAAGTGAGCGCTGCGGTGAAACAGCTGCAGGCTCGCGGCGCGCATTACTTCACCAAGCGGATTGATACCACGC
TGCGCGGCGGCATCGGATTTGAAATCGACGCCATGCTGGAACAACTGCCGCAGGAGACGGTGGCGGTTGTGGTGCCGGCG
ATGCCGCAGTCACGGCGTATTCTCGTCGGCGGTTACTCGGTGATTGATTCGGTAGCCCTGTCGCGGACCGATGTGGCTCG
CGATGTACGTACCCCGGTCACCGAGTCGTGGGTGCCGGGACTGTTGGCGGCGCAGACCCATCATCAGGTCGGGCATATCG
CGCTGACATCGGTGATGAAAGGCGAAGGGCAAATCCAGCAGGATTTACAGGAACAACAACAGCGCGGCGTGCGGGTGATA
GTGGTCGATGCGATAACCGTCGACGATGTCGATGCCATCGCCGGGGCGGTAGTGGCCCTGAACTGGAACGTGCTGGCCGT
CGATCCGGGGCCCTTTACCGAACGGCTGGCGGTTCGTCGCGGCCTGATGCGCGAAGCGCGGAGCAGCGCGCCCGCGAGCC
TGACAGCGGACGGCCAGCGCGGCTCGATTTTGATCGTTGCCGGCAGCGCAACGCCAGTAACCAAAAAACAGCTCCAGTAT
TTGATCGCTAATGATGCCCGGGTCTGCCATATCCCGGTGGATGCCGAGCTGTTGGTTGACCGGAAAAACGCCGCTGAGAT
TGAAGTCAATCGCGTGGTGCAGCATGCCCGACAGTGCGTTCCGGCGCAGCACAACGCGCTCTTTGTTTTTGAGTCCGCCT
TGACCGGGCGGTTGCTAAACCTTCAGGAAGAGGAGCAGCGATTTGGTCTGGCCCACGGTGAGGCTGCGCAAAATATTAAC
CACGGATTGGGCAGTATTGTGCGCGAGGTGCTTAACTGTGCGTCGGGTGAAATTAAAGGATTGTATATGACCGGTGGTGA
CACCATGGTCAATGTACTAAAAGAGCTGGGCGCCACCGGAATAGAAATGATTGACTATGTCATCCCGCAAACCGATATGG
TGCGGATCATTGGCGGCGATTATGCCGGCTTAATTTGCGTCGGGAAAGGAGGGCTAACCGGACCGGAAGATATTATTAGC
ATCATTGTTGACCGAATTTATCAGGAAGCACAGCAATAA

Upstream 100 bases:

>100_bases
GTACCGTCAGTATGCTGGAGTCGACGCTGGTGGCGGCCCGTTACTGGAAAATGAAGCATCAATAATCGCTGAGGTTCATG
CGAATCACGAGGACAGAGAA

Downstream 100 bases:

>100_bases
CCCAGTAATCTAATAACTAATAAACGACGTAATGGTCGTCAGGGTAGTATTGCGTCGTTATCTCTGTTAAATCTTAATTT
AAGAGTTACCCTATGAATAT

Product: putative glucitol/sorbitol-specific PTS family enzyme IIBC component

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MEKHLLISKGCKGWGGPLTITPGQGKKIAYITGGIRPPVVDRLSELTGWPSIDVFKNGEPPAEEIGLMVIDCGGTLRCGL
YPKRGIPTINLHPTGKSGPLAEFIHEGIYVSGVTPACIEMVWPQGEGGKLGIVADDLTGATTVGVLLARSGLKTAAFFDT
ESFARNEVEYPAMVVSSDSRPLPKAEAQHKVSAAVKQLQARGAHYFTKRIDTTLRGGIGFEIDAMLEQLPQETVAVVVPA
MPQSRRILVGGYSVIDSVALSRTDVARDVRTPVTESWVPGLLAAQTHHQVGHIALTSVMKGEGQIQQDLQEQQQRGVRVI
VVDAITVDDVDAIAGAVVALNWNVLAVDPGPFTERLAVRRGLMREARSSAPASLTADGQRGSILIVAGSATPVTKKQLQY
LIANDARVCHIPVDAELLVDRKNAAEIEVNRVVQHARQCVPAQHNALFVFESALTGRLLNLQEEEQRFGLAHGEAAQNIN
HGLGSIVREVLNCASGEIKGLYMTGGDTMVNVLKELGATGIEMIDYVIPQTDMVRIIGGDYAGLICVGKGGLTGPEDIIS
IIVDRIYQEAQQ

Sequences:

>Translated_572_residues
MEKHLLISKGCKGWGGPLTITPGQGKKIAYITGGIRPPVVDRLSELTGWPSIDVFKNGEPPAEEIGLMVIDCGGTLRCGL
YPKRGIPTINLHPTGKSGPLAEFIHEGIYVSGVTPACIEMVWPQGEGGKLGIVADDLTGATTVGVLLARSGLKTAAFFDT
ESFARNEVEYPAMVVSSDSRPLPKAEAQHKVSAAVKQLQARGAHYFTKRIDTTLRGGIGFEIDAMLEQLPQETVAVVVPA
MPQSRRILVGGYSVIDSVALSRTDVARDVRTPVTESWVPGLLAAQTHHQVGHIALTSVMKGEGQIQQDLQEQQQRGVRVI
VVDAITVDDVDAIAGAVVALNWNVLAVDPGPFTERLAVRRGLMREARSSAPASLTADGQRGSILIVAGSATPVTKKQLQY
LIANDARVCHIPVDAELLVDRKNAAEIEVNRVVQHARQCVPAQHNALFVFESALTGRLLNLQEEEQRFGLAHGEAAQNIN
HGLGSIVREVLNCASGEIKGLYMTGGDTMVNVLKELGATGIEMIDYVIPQTDMVRIIGGDYAGLICVGKGGLTGPEDIIS
IIVDRIYQEAQQ
>Mature_572_residues
MEKHLLISKGCKGWGGPLTITPGQGKKIAYITGGIRPPVVDRLSELTGWPSIDVFKNGEPPAEEIGLMVIDCGGTLRCGL
YPKRGIPTINLHPTGKSGPLAEFIHEGIYVSGVTPACIEMVWPQGEGGKLGIVADDLTGATTVGVLLARSGLKTAAFFDT
ESFARNEVEYPAMVVSSDSRPLPKAEAQHKVSAAVKQLQARGAHYFTKRIDTTLRGGIGFEIDAMLEQLPQETVAVVVPA
MPQSRRILVGGYSVIDSVALSRTDVARDVRTPVTESWVPGLLAAQTHHQVGHIALTSVMKGEGQIQQDLQEQQQRGVRVI
VVDAITVDDVDAIAGAVVALNWNVLAVDPGPFTERLAVRRGLMREARSSAPASLTADGQRGSILIVAGSATPVTKKQLQY
LIANDARVCHIPVDAELLVDRKNAAEIEVNRVVQHARQCVPAQHNALFVFESALTGRLLNLQEEEQRFGLAHGEAAQNIN
HGLGSIVREVLNCASGEIKGLYMTGGDTMVNVLKELGATGIEMIDYVIPQTDMVRIIGGDYAGLICVGKGGLTGPEDIIS
IIVDRIYQEAQQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3395

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=118, Percent_Identity=64.4067796610169, Blast_Score=159, Evalue=6e-40,
Organism=Escherichia coli, GI1789093, Length=202, Percent_Identity=31.1881188118812, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61215; Mature: 61215

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKHLLISKGCKGWGGPLTITPGQGKKIAYITGGIRPPVVDRLSELTGWPSIDVFKNGEP
CCCCEEEECCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCC
PAEEIGLMVIDCGGTLRCGLYPKRGIPTINLHPTGKSGPLAEFIHEGIYVSGVTPACIEM
CHHHCCEEEEECCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHCCEEEECCCHHHHHE
VWPQGEGGKLGIVADDLTGATTVGVLLARSGLKTAAFFDTESFARNEVEYPAMVVSSDSR
ECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCEEEEECCCC
PLPKAEAQHKVSAAVKQLQARGAHYFTKRIDTTLRGGIGFEIDAMLEQLPQETVAVVVPA
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECC
MPQSRRILVGGYSVIDSVALSRTDVARDVRTPVTESWVPGLLAAQTHHQVGHIALTSVMK
CCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHC
GEGQIQQDLQEQQQRGVRVIVVDAITVDDVDAIAGAVVALNWNVLAVDPGPFTERLAVRR
CCCHHHHHHHHHHHCCCEEEEEEEEEECCHHHHCCEEEEEECEEEEECCCCHHHHHHHHH
GLMREARSSAPASLTADGQRGSILIVAGSATPVTKKQLQYLIANDARVCHIPVDAELLVD
HHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEE
RKNAAEIEVNRVVQHARQCVPAQHNALFVFESALTGRLLNLQEEEQRFGLAHGEAAQNIN
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHCHHCCCCHHHHHHCCCCCHHHHHHH
HGLGSIVREVLNCASGEIKGLYMTGGDTMVNVLKELGATGIEMIDYVIPQTDMVRIIGGD
HHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCC
YAGLICVGKGGLTGPEDIISIIVDRIYQEAQQ
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKHLLISKGCKGWGGPLTITPGQGKKIAYITGGIRPPVVDRLSELTGWPSIDVFKNGEP
CCCCEEEECCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCC
PAEEIGLMVIDCGGTLRCGLYPKRGIPTINLHPTGKSGPLAEFIHEGIYVSGVTPACIEM
CHHHCCEEEEECCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHCCEEEECCCHHHHHE
VWPQGEGGKLGIVADDLTGATTVGVLLARSGLKTAAFFDTESFARNEVEYPAMVVSSDSR
ECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCEEEEECCCC
PLPKAEAQHKVSAAVKQLQARGAHYFTKRIDTTLRGGIGFEIDAMLEQLPQETVAVVVPA
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECC
MPQSRRILVGGYSVIDSVALSRTDVARDVRTPVTESWVPGLLAAQTHHQVGHIALTSVMK
CCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHC
GEGQIQQDLQEQQQRGVRVIVVDAITVDDVDAIAGAVVALNWNVLAVDPGPFTERLAVRR
CCCHHHHHHHHHHHCCCEEEEEEEEEECCHHHHCCEEEEEECEEEEECCCCHHHHHHHHH
GLMREARSSAPASLTADGQRGSILIVAGSATPVTKKQLQYLIANDARVCHIPVDAELLVD
HHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEE
RKNAAEIEVNRVVQHARQCVPAQHNALFVFESALTGRLLNLQEEEQRFGLAHGEAAQNIN
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHCHHCCCCHHHHHHCCCCCHHHHHHH
HGLGSIVREVLNCASGEIKGLYMTGGDTMVNVLKELGATGIEMIDYVIPQTDMVRIIGGD
HHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCC
YAGLICVGKGGLTGPEDIISIIVDRIYQEAQQ
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9435786 [H]