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The map label for this gene is dosC [H]

Identifier: 238894037

GI number: 238894037

Start: 1935863

End: 1936783

Strand: Direct

Name: dosC [H]

Synonym: KP1_1983

Alternate gene names: 238894037

Gene position: 1935863-1936783 (Clockwise)

Preceding gene: 238894036

Following gene: 238894039

Centisome position: 36.88

GC content: 58.2

Gene sequence:

>921_bases
ATGCCTGAACTCCATACTCCTGCGAATCGCCCCGGCCCGGCCGCTGTGGCCCGGGTGACGCTCCTGCCTGCGCTGCTGAT
TATCATTGCTGTTGCTGCCGGCTGCGCCCTGGTGTCGCCTCCGGTCGGTACACGACACGAGATCCTGAATAACCCCGGCC
TCTATATCGATCTGCTGGCGTTGTTGTTTCTGGTGTTTATGCTGTGGAGCTCGGCGAAGGTGCGGATGAGCCACATTGCC
GTCAACTGCGTACGCTATGGGCTGCTGTTGTGGATTGCCGGCGGCACCTTTGACGTGATGGATGAGATCGTGGTTCAGCC
GCGCTGGATGGGCTACTACTGCGAAGATCTGCTGCGCCTCTCCGGGATGCTGCTTACCGTGGTGGGGGTCTACAAAATTA
TTGAGCGAATCAATTTGCTTTACGTCGATGCGCGCTCGCAATCCCTGAAGGATGAACTGACCCAGTTACCCAACCGGCGT
TTTTTCATTGATACCATTCGCGAGAAGTCCGGGCATGCGCTGGGACTCATGATTCTGGATATCGATTTTTTCAAGAAAAT
CAACGATAGCTGGGGCCACCTGGTGGGGGATGAAGTGTTATTTTCTCTGGGGAAACAGCTGGCGAAACTAACCAGCGAGC
AGGTCCAGCCGGCGCGAATCGGTGGGGAAGAGTTCGCCATTATCGTCGATGGCCTGTCGGAGCCCGAGCTGCACGCTCTG
GCGCAGTCGATCCTTGCTAACGCCCGCACCATCCTGATCAATCATCAGACGCCGCTGTCGATCAGCATTGGCGTCGGAAC
CTGGCGGCCAGGCGAAGCGCAGGAGACGTTTATCCGTAAAGTCGACGAGGCGCTGTACAAGGCCAAGCACAACGGCCGCG
GCCGTGTGGAGTGGGCGCCATCTACCGGTGCGGCAGGTTAG

Upstream 100 bases:

>100_bases
TCCCGCCATTTTACCGAACCGTCGCTTTTTCATTCGTCCCTCTACGCTTAACTGTTATCCTGTCTCGCGATCGGCCCTGT
TGTTACCATGAGTCACCGCT

Downstream 100 bases:

>100_bases
GCCTGCTGCGCGCTACGGCAGTGTCGCCGATACTCCGATGGCGAGCGTTCTGTGTAGCGTTTAAAAACCCGGCAAAAATA
GTTGCTGTCGACAAAGCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPELHTPANRPGPAAVARVTLLPALLIIIAVAAGCALVSPPVGTRHEILNNPGLYIDLLALLFLVFMLWSSAKVRMSHIA
VNCVRYGLLLWIAGGTFDVMDEIVVQPRWMGYYCEDLLRLSGMLLTVVGVYKIIERINLLYVDARSQSLKDELTQLPNRR
FFIDTIREKSGHALGLMILDIDFFKKINDSWGHLVGDEVLFSLGKQLAKLTSEQVQPARIGGEEFAIIVDGLSEPELHAL
AQSILANARTILINHQTPLSISIGVGTWRPGEAQETFIRKVDEALYKAKHNGRGRVEWAPSTGAAG

Sequences:

>Translated_306_residues
MPELHTPANRPGPAAVARVTLLPALLIIIAVAAGCALVSPPVGTRHEILNNPGLYIDLLALLFLVFMLWSSAKVRMSHIA
VNCVRYGLLLWIAGGTFDVMDEIVVQPRWMGYYCEDLLRLSGMLLTVVGVYKIIERINLLYVDARSQSLKDELTQLPNRR
FFIDTIREKSGHALGLMILDIDFFKKINDSWGHLVGDEVLFSLGKQLAKLTSEQVQPARIGGEEFAIIVDGLSEPELHAL
AQSILANARTILINHQTPLSISIGVGTWRPGEAQETFIRKVDEALYKAKHNGRGRVEWAPSTGAAG
>Mature_305_residues
PELHTPANRPGPAAVARVTLLPALLIIIAVAAGCALVSPPVGTRHEILNNPGLYIDLLALLFLVFMLWSSAKVRMSHIAV
NCVRYGLLLWIAGGTFDVMDEIVVQPRWMGYYCEDLLRLSGMLLTVVGVYKIIERINLLYVDARSQSLKDELTQLPNRRF
FIDTIREKSGHALGLMILDIDFFKKINDSWGHLVGDEVLFSLGKQLAKLTSEQVQPARIGGEEFAIIVDGLSEPELHALA
QSILANARTILINHQTPLSISIGVGTWRPGEAQETFIRKVDEALYKAKHNGRGRVEWAPSTGAAG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=196, Percent_Identity=33.6734693877551, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=32.9192546583851, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787056, Length=152, Percent_Identity=36.8421052631579, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=36.3057324840764, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786584, Length=160, Percent_Identity=35, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787541, Length=154, Percent_Identity=37.6623376623377, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=156, Percent_Identity=32.6923076923077, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788956, Length=152, Percent_Identity=36.1842105263158, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081881, Length=152, Percent_Identity=33.5526315789474, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082007, Length=186, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788085, Length=190, Percent_Identity=25.2631578947368, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33724; Mature: 33593

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELHTPANRPGPAAVARVTLLPALLIIIAVAAGCALVSPPVGTRHEILNNPGLYIDLLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHHCCCCHHHHHHH
LLFLVFMLWSSAKVRMSHIAVNCVRYGLLLWIAGGTFDVMDEIVVQPRWMGYYCEDLLRL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
SGMLLTVVGVYKIIERINLLYVDARSQSLKDELTQLPNRRFFIDTIREKSGHALGLMILD
HHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCCCCEEEHHHHHCCCCCEEEEEEEE
IDFFKKINDSWGHLVGDEVLFSLGKQLAKLTSEQVQPARIGGEEFAIIVDGLSEPELHAL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCCHHHHHH
AQSILANARTILINHQTPLSISIGVGTWRPGEAQETFIRKVDEALYKAKHNGRGRVEWAP
HHHHHCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECC
STGAAG
CCCCCC
>Mature Secondary Structure 
PELHTPANRPGPAAVARVTLLPALLIIIAVAAGCALVSPPVGTRHEILNNPGLYIDLLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHHCCCCHHHHHHH
LLFLVFMLWSSAKVRMSHIAVNCVRYGLLLWIAGGTFDVMDEIVVQPRWMGYYCEDLLRL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
SGMLLTVVGVYKIIERINLLYVDARSQSLKDELTQLPNRRFFIDTIREKSGHALGLMILD
HHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCCCCEEEHHHHHCCCCCEEEEEEEE
IDFFKKINDSWGHLVGDEVLFSLGKQLAKLTSEQVQPARIGGEEFAIIVDGLSEPELHAL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCCHHHHHH
AQSILANARTILINHQTPLSISIGVGTWRPGEAQETFIRKVDEALYKAKHNGRGRVEWAP
HHHHHCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECC
STGAAG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]