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The map label for this gene is helD [H]

Identifier: 238894018

GI number: 238894018

Start: 1917076

End: 1919130

Strand: Direct

Name: helD [H]

Synonym: KP1_1964

Alternate gene names: 238894018

Gene position: 1917076-1919130 (Clockwise)

Preceding gene: 238894015

Following gene: 238894020

Centisome position: 36.53

GC content: 57.66

Gene sequence:

>2055_bases
ATGGAACTTAAAGCGACAACATTGGGCAAACGCATGGCTCAGCACCCTTATGATCGGGTGCAGCTACTGAACGCCGGGGT
AAAAGTTTCCGGCGATCGTCATGAGTATCTTATACCTTTCAATCAGTTATTGTCAGTGCATTGTAAGCGCGGTCTGGTCT
GGGGCGAACTGGAGTTTGTGCTACCGGACGGGAAAGTCGTGCGTTTGCACGGTACCGAGTGGAGCGAAACGCAGCGCTTT
TATCATCATTTACATACGCTCTGGCAGCAGTGGAGCACGGAGATGAGCGATATCGCCGCCGGCGTGCTGAAGCAGCAGCT
GGCGACGATCGAGCACACTCGCGCAGAAGGCAAGTGGCTAACCCGTCAGCAGGTGGCGGATGTTCAGGATAATATACGCC
ATGCGTTGACTGGTTTGCCGATGCCCACCAGTCGGCTTGATGCCTTTGATAACTGCCGGGAGCTGTGGCGGGAGTGTCAG
CGCTGGTTAGGCGATATTGAAGCGACGCGTCTGGCCCATAACCAGGCCTTTACCGAGGCGATGCTGGAGCAATATCGCGA
ATTTTTTGATAGCGTTGAGTCGTCCCCGCTCAATGCGTCACAAGCGCGTGCGGTAGTGAACGGCGAGCGCTCCCTGCTGG
TGCTGGCGGGAGCGGGCAGCGGTAAAACCTCGGTGCTGGTGGCGCGAGCGGGCTGGCTGCTGGCGCGGGGAGAAGCCGCG
GCAGATCAAATTTTGCTGCTGGCATTCGGCCGCCAGGCGGCGCAGGAAATGGATGAGCGGATCCGCGAGCGTCTGGCATC
AGATGATATTACCGCGCGTACTTTCCATTCGCTGGCGTTGCATATTATTCAGCAGGGCAGCAAAAAAGTGCCCACTATCA
GTAAGCTGGAAAGCGATACTGCGGCCCGCCACGCATTGCTGCTGAAAAGCTGGCAAAAGCAGTGCCAGGAGAAAAAAGCG
CAGGCGAAAGGTTGGCGCCTGTGGCTGGAAGAAGAGATGGGCTGGCAACTGCCGGAGGGGGACTTCTGGCAGGACAAAAA
GGTGCAGCGGCGGATGGCCAGCCGGCTTGACCGCTGGGTGAGCCTGATGCGCATGCACGGCGGCTCCCAGGCGGAGATGA
TTGCCGGGGCGCCGGAAGCGGTACGCGACCTGTTCAGTAAACGGGTGAAGCTGATGTCGCCTCTGATGAAAGACTGGAAG
GCGGCGCTCAAGGCCGAGAATGCGGTCGATTTTTCCGGCCTGATCCATCAGGCGGTCAATATTCTCGATAAAGGCCGCTT
CGTCAGCCCTTGGAAACACATTCTGGTGGATGAATTTCAAGATATTTCGCCGCAGCGAGCCTCGCTGTTGGCCGCGTTAC
GCCGGCAGAATAGCCAAACCACGCTCTTTGCCGTCGGGGATGACTGGCAGGCGATCTATCGGTTCAGCGGGGCGCAGCTT
TCTCTGACCACCGCATTCAACCACTACTTTGGCGAAGGGGATTGCTGTGCGCTGGATACCACCTATCGATTTAACGGGCG
TATCGGCGAAATAGCCAATGGCTTTATCCAGCAAAATCCGCATCAGTTAAGCAAACCGCTGAACAGCCTTACGGCAGGTG
ATAAAAAGGCCGTCACGCTGTTAGCCGACGACAAGCTGGACGATCTTCTGGATAAGCTGAGCGGTTACGTTAAGCCCGAG
CAGCGCATCCTGCTGCTTGCCCGTTATCACCATCTGAAGCCTGAGGCGCTCAACAAAGCGGCGACGCGCTGGCCGCACCT
GCAGCTTGATTTTATGACGATTCATGCCAGCAAAGGTCAGCAGGCTGACTTTGTCATTGTGTTGGGACTACAGGATGGGG
AGGATGCGTTTCCGGCCCCGGCGCGCGAGTCGATTATGGAGCAGGCGCTCCTGCCGCAGCCGGAAGATTTTCCGGATGCG
GAGGAGCGTAGACTGTTGTACGTGGCGCTCACCCGCGCTCGTCACCGGGTATGGCTGTTATTTAACAAGGCGCAACCTTC
GCCGTTTGTCGAGATCCTGCAGGCGCTGGATGTGCCCGTGGCCAGGAAGCCGTAA

Upstream 100 bases:

>100_bases
TAATAAGACCTGATGATTGTAACGTTTTTTTGCGCTGGAGCACCTGGTCCGGGGCGGTTAAACTGAAAAACAATCCACCC
TGATTGAAAATCGATATTAT

Downstream 100 bases:

>100_bases
TCGCGGCTCGATTATTTCAGGCGCTCGGCAAGATAGCGTGGATAATCCGGGATCAGAATATCGAGCGGTTGACCGAAGTG
CGGCGACTGGATAATAAAGT

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MELKATTLGKRMAQHPYDRVQLLNAGVKVSGDRHEYLIPFNQLLSVHCKRGLVWGELEFVLPDGKVVRLHGTEWSETQRF
YHHLHTLWQQWSTEMSDIAAGVLKQQLATIEHTRAEGKWLTRQQVADVQDNIRHALTGLPMPTSRLDAFDNCRELWRECQ
RWLGDIEATRLAHNQAFTEAMLEQYREFFDSVESSPLNASQARAVVNGERSLLVLAGAGSGKTSVLVARAGWLLARGEAA
ADQILLLAFGRQAAQEMDERIRERLASDDITARTFHSLALHIIQQGSKKVPTISKLESDTAARHALLLKSWQKQCQEKKA
QAKGWRLWLEEEMGWQLPEGDFWQDKKVQRRMASRLDRWVSLMRMHGGSQAEMIAGAPEAVRDLFSKRVKLMSPLMKDWK
AALKAENAVDFSGLIHQAVNILDKGRFVSPWKHILVDEFQDISPQRASLLAALRRQNSQTTLFAVGDDWQAIYRFSGAQL
SLTTAFNHYFGEGDCCALDTTYRFNGRIGEIANGFIQQNPHQLSKPLNSLTAGDKKAVTLLADDKLDDLLDKLSGYVKPE
QRILLLARYHHLKPEALNKAATRWPHLQLDFMTIHASKGQQADFVIVLGLQDGEDAFPAPARESIMEQALLPQPEDFPDA
EERRLLYVALTRARHRVWLLFNKAQPSPFVEILQALDVPVARKP

Sequences:

>Translated_684_residues
MELKATTLGKRMAQHPYDRVQLLNAGVKVSGDRHEYLIPFNQLLSVHCKRGLVWGELEFVLPDGKVVRLHGTEWSETQRF
YHHLHTLWQQWSTEMSDIAAGVLKQQLATIEHTRAEGKWLTRQQVADVQDNIRHALTGLPMPTSRLDAFDNCRELWRECQ
RWLGDIEATRLAHNQAFTEAMLEQYREFFDSVESSPLNASQARAVVNGERSLLVLAGAGSGKTSVLVARAGWLLARGEAA
ADQILLLAFGRQAAQEMDERIRERLASDDITARTFHSLALHIIQQGSKKVPTISKLESDTAARHALLLKSWQKQCQEKKA
QAKGWRLWLEEEMGWQLPEGDFWQDKKVQRRMASRLDRWVSLMRMHGGSQAEMIAGAPEAVRDLFSKRVKLMSPLMKDWK
AALKAENAVDFSGLIHQAVNILDKGRFVSPWKHILVDEFQDISPQRASLLAALRRQNSQTTLFAVGDDWQAIYRFSGAQL
SLTTAFNHYFGEGDCCALDTTYRFNGRIGEIANGFIQQNPHQLSKPLNSLTAGDKKAVTLLADDKLDDLLDKLSGYVKPE
QRILLLARYHHLKPEALNKAATRWPHLQLDFMTIHASKGQQADFVIVLGLQDGEDAFPAPARESIMEQALLPQPEDFPDA
EERRLLYVALTRARHRVWLLFNKAQPSPFVEILQALDVPVARKP
>Mature_684_residues
MELKATTLGKRMAQHPYDRVQLLNAGVKVSGDRHEYLIPFNQLLSVHCKRGLVWGELEFVLPDGKVVRLHGTEWSETQRF
YHHLHTLWQQWSTEMSDIAAGVLKQQLATIEHTRAEGKWLTRQQVADVQDNIRHALTGLPMPTSRLDAFDNCRELWRECQ
RWLGDIEATRLAHNQAFTEAMLEQYREFFDSVESSPLNASQARAVVNGERSLLVLAGAGSGKTSVLVARAGWLLARGEAA
ADQILLLAFGRQAAQEMDERIRERLASDDITARTFHSLALHIIQQGSKKVPTISKLESDTAARHALLLKSWQKQCQEKKA
QAKGWRLWLEEEMGWQLPEGDFWQDKKVQRRMASRLDRWVSLMRMHGGSQAEMIAGAPEAVRDLFSKRVKLMSPLMKDWK
AALKAENAVDFSGLIHQAVNILDKGRFVSPWKHILVDEFQDISPQRASLLAALRRQNSQTTLFAVGDDWQAIYRFSGAQL
SLTTAFNHYFGEGDCCALDTTYRFNGRIGEIANGFIQQNPHQLSKPLNSLTAGDKKAVTLLADDKLDDLLDKLSGYVKPE
QRILLLARYHHLKPEALNKAATRWPHLQLDFMTIHASKGQQADFVIVLGLQDGEDAFPAPARESIMEQALLPQPEDFPDA
EERRLLYVALTRARHRVWLLFNKAQPSPFVEILQALDVPVARKP

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=684, Percent_Identity=78.8011695906433, Blast_Score=1139, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=341, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77577; Mature: 77577

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKATTLGKRMAQHPYDRVQLLNAGVKVSGDRHEYLIPFNQLLSVHCKRGLVWGELEFV
CCCCHHHHHHHHHHCCHHHHHHHHCCCEECCCCCEEECCHHHHHHHHHHCCCEEEEEEEE
LPDGKVVRLHGTEWSETQRFYHHLHTLWQQWSTEMSDIAAGVLKQQLATIEHTRAEGKWL
ECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
TRQQVADVQDNIRHALTGLPMPTSRLDAFDNCRELWRECQRWLGDIEATRLAHNQAFTEA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MLEQYREFFDSVESSPLNASQARAVVNGERSLLVLAGAGSGKTSVLVARAGWLLARGEAA
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCEEEECCCHH
ADQILLLAFGRQAAQEMDERIRERLASDDITARTFHSLALHIIQQGSKKVPTISKLESDT
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
AARHALLLKSWQKQCQEKKAQAKGWRLWLEEEMGWQLPEGDFWQDKKVQRRMASRLDRWV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SLMRMHGGSQAEMIAGAPEAVRDLFSKRVKLMSPLMKDWKAALKAENAVDFSGLIHQAVN
HHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ILDKGRFVSPWKHILVDEFQDISPQRASLLAALRRQNSQTTLFAVGDDWQAIYRFSGAQL
HHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCEE
SLTTAFNHYFGEGDCCALDTTYRFNGRIGEIANGFIQQNPHQLSKPLNSLTAGDKKAVTL
EEEEHHHHHCCCCCEEEEECEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEE
LADDKLDDLLDKLSGYVKPEQRILLLARYHHLKPEALNKAATRWPHLQLDFMTIHASKGQ
EECCCHHHHHHHHHCCCCCHHHEEHHHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCC
QADFVIVLGLQDGEDAFPAPARESIMEQALLPQPEDFPDAEERRLLYVALTRARHRVWLL
CCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCEEEEEEEHHCCCEEEEE
FNKAQPSPFVEILQALDVPVARKP
EECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MELKATTLGKRMAQHPYDRVQLLNAGVKVSGDRHEYLIPFNQLLSVHCKRGLVWGELEFV
CCCCHHHHHHHHHHCCHHHHHHHHCCCEECCCCCEEECCHHHHHHHHHHCCCEEEEEEEE
LPDGKVVRLHGTEWSETQRFYHHLHTLWQQWSTEMSDIAAGVLKQQLATIEHTRAEGKWL
ECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
TRQQVADVQDNIRHALTGLPMPTSRLDAFDNCRELWRECQRWLGDIEATRLAHNQAFTEA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MLEQYREFFDSVESSPLNASQARAVVNGERSLLVLAGAGSGKTSVLVARAGWLLARGEAA
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCEEEECCCHH
ADQILLLAFGRQAAQEMDERIRERLASDDITARTFHSLALHIIQQGSKKVPTISKLESDT
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
AARHALLLKSWQKQCQEKKAQAKGWRLWLEEEMGWQLPEGDFWQDKKVQRRMASRLDRWV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SLMRMHGGSQAEMIAGAPEAVRDLFSKRVKLMSPLMKDWKAALKAENAVDFSGLIHQAVN
HHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ILDKGRFVSPWKHILVDEFQDISPQRASLLAALRRQNSQTTLFAVGDDWQAIYRFSGAQL
HHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCEE
SLTTAFNHYFGEGDCCALDTTYRFNGRIGEIANGFIQQNPHQLSKPLNSLTAGDKKAVTL
EEEEHHHHHCCCCCEEEEECEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEE
LADDKLDDLLDKLSGYVKPEQRILLLARYHHLKPEALNKAATRWPHLQLDFMTIHASKGQ
EECCCHHHHHHHHHCCCCCHHHEEHHHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCC
QADFVIVLGLQDGEDAFPAPARESIMEQALLPQPEDFPDAEERRLLYVALTRARHRVWLL
CCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCEEEEEEEHHCCCEEEEE
FNKAQPSPFVEILQALDVPVARKP
EECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]