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The map label for this gene is pflA [H]

Identifier: 238893959

GI number: 238893959

Start: 1845020

End: 1845760

Strand: Reverse

Name: pflA [H]

Synonym: KP1_1900

Alternate gene names: 238893959

Gene position: 1845760-1845020 (Counterclockwise)

Preceding gene: 238893960

Following gene: 238893949

Centisome position: 35.17

GC content: 53.98

Gene sequence:

>741_bases
ATGTCAGTTATTGGTCGCATTCACTCCTTTGAATCCTGTGGCACCGTTGATGGCCCGGGCATCCGCTTTATTACCTTTTT
CCAGGGCTGCCTGATGCGCTGCCTGTACTGCCATAACCGTGACACCTGGGATACCCACGGCGGCAAAGAAATCACCGTTG
AAGAATTAATGAAAGAGGTGGTGACCTATCGTCACTTTATGAATGCTTCCGGCGGCGGCGTCACCGCCTCGGGCGGTGAG
GCGATCCTGCAGGCGGAGTTTGTTCGCGACTGGTTCCGCGCGTGTAAGAAAGAAGGCATCCACACCTGCCTGGATACCAA
CGGCTTCGTACGTCGCTACGATCCGGTTATCGACGAGCTGCTGGAGGTAACAGACCTGGTGATGCTGGATCTCAAGCAGA
TGAACGATGAGATTCACCAGAACCTGGTGGGCGTCTCTAACCATCGTACGCTGGAATTCGCCCAGTATCTGGCGAAGAAG
AATATCAATGTCTGGATCCGCTACGTGGTGGTTCCCGGCTGGTCAGACGATGATGATTCCGCGCACCGCCTGGGCGAATT
TACCCGCGATATGGGCAATGTCGAAAAAATCGAGCTGCTGCCCTACCATGAGCTGGGTAAACACAAATGGGTAGCGATGG
GCGAAGAGTACAAGCTGGACGGCGTCCATCCGCCGAAGAAAGAGACCATGGAGCGGGTAAAAGGCATCCTTGAGCAGTAT
GGTCACAAAGTAATGTACTAA

Upstream 100 bases:

>100_bases
TTATCTATACTTTGGGTACCTGTCAAAACAGACTCGACGCAGCCGCTGAGCTGCGCACCAACACGGCCCCGGATGGGCCA
CATCTGGAGAAAACACCGCA

Downstream 100 bases:

>100_bases
TCCCGCCCGGGCCGGTGATGCGCTTATGCCGGCCTGCAACGGCGTGGGAAGCTGTGTCCGCCCCGCCGTTTCATTCTCAT
GAAAAACGACTTGTCCTCTT

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEITVEELMKEVVTYRHFMNASGGGVTASGGE
AILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAQYLAKK
NINVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVHPPKKETMERVKGILEQY
GHKVMY

Sequences:

>Translated_246_residues
MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEITVEELMKEVVTYRHFMNASGGGVTASGGE
AILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAQYLAKK
NINVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVHPPKKETMERVKGILEQY
GHKVMY
>Mature_245_residues
SVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEITVEELMKEVVTYRHFMNASGGGVTASGGEA
ILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAQYLAKKN
INVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVHPPKKETMERVKGILEQYG
HKVMY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=96.7479674796748, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=26.2773722627737, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1790839, Length=268, Percent_Identity=27.9850746268657, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI226510931, Length=174, Percent_Identity=32.183908045977, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28272; Mature: 28140

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEITVEELMKEV
CCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHH
VTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAQYLAKKNINVWIRYVVVPGWSDDDDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCHH
AHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVHPPKKETMERVKGILEQY
HHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCCCCCCHHHHHHHHHHHHHHH
GHKVMY
CCCCCC
>Mature Secondary Structure 
SVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEITVEELMKEV
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHH
VTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAQYLAKKNINVWIRYVVVPGWSDDDDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCHH
AHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVHPPKKETMERVKGILEQY
HHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCCCCCCHHHHHHHHHHHHHHH
GHKVMY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]