Click here to switch to the map view.

The map label for this gene is ybjI [H]

Identifier: 238893903

GI number: 238893903

Start: 1781043

End: 1781855

Strand: Reverse

Name: ybjI [H]

Synonym: KP1_1837

Alternate gene names: 238893903

Gene position: 1781855-1781043 (Counterclockwise)

Preceding gene: 238893904

Following gene: 238893901

Centisome position: 33.95

GC content: 57.07

Gene sequence:

>813_bases
ATGAGCATCAAACTGATTGCGGTGGATATGGACGGCACCTTTTTAAGCGATGCCAAAACTTACAACCGGCCGCGCTTCCT
CGCCCAGTACCAGCGAATGCGCGAACAGAATATTCGCTTCGTGGTCGCCAGCGGCAATCAATATTATCAGCTGATCTCCT
TTTTCCCGGAGATCGCCCATCAGATCGCCTTCGTGGCGGAAAACGGCGGCTGGGTGGTGAGCGGCAATGAAGACGTCTTC
AACTGCCAACTGCCGGTTCACCACTTCAACGCCGTGGTCGACCATCTGCAGACGTTGCCGAATATCGAAATCATCGCCTG
CGGGAAGCGCAGCGCCTACACCCTCAATCGCTACAACGACGCGCTGAAGACGGTGGCGGCGAAGTATTATCACCGCCTTG
AACTGGTGGACGATTTTAACCATCTCGATGACACCATCCTCAAATTTGGTCTCAACGTGCCAGACAGCCTGATCCCGGAA
ATTCAGCCGAAGCTGCACGCGGCGCTGGGGGACATGGTCACCGCGGTGGCCACCGGCTACGGCAGCATCGACTTAATTAT
CCCGGGTGTGCACAAAGCCAACGGGCTGCGCATTTTGCAGCAGCGCTGGGGCATTGAGGATCACGAGGTGGTGGCGTTCG
GCGACAGCGGCAACGATATCGAAATGCTGCAGCACGCCGGGTTTGGCTTTGCCATGGCCAACGCCCGGGAAGACGTCAAA
GCCGTCGCCCGCTATCAGGCGCCGCATAACAACGAAGAAGGTGTCCTGCAGATTATTGATAAGGTGTTGGATCGCGAAGC
GCCGTTCGCCTGA

Upstream 100 bases:

>100_bases
GCAGCGCCATGATGGTGGTGCTGGGTCTGGTGATGGTGGCGGCGCTGGTCGCCCGCGCGGTGGCTAAGCCACAATCTGAA
CCCGTTATGGAGAACAGCTA

Downstream 100 bases:

>100_bases
TAAGACCCGGATGCGAGCGCATCCGGGTTGAAAACAACTACCCCTGCTGCGAATTGCCGACGCTTTTATCTTTCAGAAAG
TAAATCATCAGCCCCAGCCA

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSIKLIAVDMDGTFLSDAKTYNRPRFLAQYQRMREQNIRFVVASGNQYYQLISFFPEIAHQIAFVAENGGWVVSGNEDVF
NCQLPVHHFNAVVDHLQTLPNIEIIACGKRSAYTLNRYNDALKTVAAKYYHRLELVDDFNHLDDTILKFGLNVPDSLIPE
IQPKLHAALGDMVTAVATGYGSIDLIIPGVHKANGLRILQQRWGIEDHEVVAFGDSGNDIEMLQHAGFGFAMANAREDVK
AVARYQAPHNNEEGVLQIIDKVLDREAPFA

Sequences:

>Translated_270_residues
MSIKLIAVDMDGTFLSDAKTYNRPRFLAQYQRMREQNIRFVVASGNQYYQLISFFPEIAHQIAFVAENGGWVVSGNEDVF
NCQLPVHHFNAVVDHLQTLPNIEIIACGKRSAYTLNRYNDALKTVAAKYYHRLELVDDFNHLDDTILKFGLNVPDSLIPE
IQPKLHAALGDMVTAVATGYGSIDLIIPGVHKANGLRILQQRWGIEDHEVVAFGDSGNDIEMLQHAGFGFAMANAREDVK
AVARYQAPHNNEEGVLQIIDKVLDREAPFA
>Mature_269_residues
SIKLIAVDMDGTFLSDAKTYNRPRFLAQYQRMREQNIRFVVASGNQYYQLISFFPEIAHQIAFVAENGGWVVSGNEDVFN
CQLPVHHFNAVVDHLQTLPNIEIIACGKRSAYTLNRYNDALKTVAAKYYHRLELVDDFNHLDDTILKFGLNVPDSLIPEI
QPKLHAALGDMVTAVATGYGSIDLIIPGVHKANGLRILQQRWGIEDHEVVAFGDSGNDIEMLQHAGFGFAMANAREDVKA
VARYQAPHNNEEGVLQIIDKVLDREAPFA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=269, Percent_Identity=70.2602230483271, Blast_Score=393, Evalue=1e-111,
Organism=Escherichia coli, GI1787043, Length=269, Percent_Identity=51.3011152416357, Blast_Score=289, Evalue=1e-79,
Organism=Escherichia coli, GI2367265, Length=268, Percent_Identity=25.3731343283582, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30253; Mature: 30122

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLIAVDMDGTFLSDAKTYNRPRFLAQYQRMREQNIRFVVASGNQYYQLISFFPEIAH
CCEEEEEEECCCCEECCHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
QIAFVAENGGWVVSGNEDVFNCQLPVHHFNAVVDHLQTLPNIEIIACGKRSAYTLNRYND
HEEEEEECCCEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHH
ALKTVAAKYYHRLELVDDFNHLDDTILKFGLNVPDSLIPEIQPKLHAALGDMVTAVATGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCC
GSIDLIIPGVHKANGLRILQQRWGIEDHEVVAFGDSGNDIEMLQHAGFGFAMANAREDVK
CCEEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCEEECCHHHHHH
AVARYQAPHNNEEGVLQIIDKVLDREAPFA
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SIKLIAVDMDGTFLSDAKTYNRPRFLAQYQRMREQNIRFVVASGNQYYQLISFFPEIAH
CEEEEEEECCCCEECCHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
QIAFVAENGGWVVSGNEDVFNCQLPVHHFNAVVDHLQTLPNIEIIACGKRSAYTLNRYND
HEEEEEECCCEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHH
ALKTVAAKYYHRLELVDDFNHLDDTILKFGLNVPDSLIPEIQPKLHAALGDMVTAVATGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCC
GSIDLIIPGVHKANGLRILQQRWGIEDHEVVAFGDSGNDIEMLQHAGFGFAMANAREDVK
CCEEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCEEECCHHHHHH
AVARYQAPHNNEEGVLQIIDKVLDREAPFA
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]