Click here to switch to the map view.

The map label for this gene is rna [H]

Identifier: 238893688

GI number: 238893688

Start: 1551149

End: 1551946

Strand: Reverse

Name: rna [H]

Synonym: KP1_1607

Alternate gene names: 238893688

Gene position: 1551946-1551149 (Counterclockwise)

Preceding gene: 238893689

Following gene: 238893687

Centisome position: 29.57

GC content: 60.15

Gene sequence:

>798_bases
ATGTTCAGGAAGGATTTCGCCGCGATTGCCCTTGTGCTTACCGCCACTCAGGCCGGTGCTGAACCGCTCACCGCGACGCG
ATACGCTGATTTTGACCGCTACGTCCTTGCCCTCTCCTGGCAAACCGGATTCTGTCAGAGCATGTATGACCGCAATCGCA
ACGAACCGGAAGAGTGTCGCCTGCAGCAGGACACCGCGAATAAAGCCGACTTTCTGACCGTGCATGGCCTGTGGCCCGGG
CTGCCAAAGTCCATTGCCGCGCGCGGAGTGGATGAACGTCGCTGGATGCGCTACGGCTGCGCCACGCGTCCGGTGCCCAA
CATGCCGGAGGTAAAAGCGAACCGCAAATGCCAGGCGGCGGAGACCGGACTGTCGCTGGAGATGGCCAATAAACTTAATA
ATGTGATGCCGGGATCGGGCGGCACCTCCTGTCTGGAACGCTACGAATATGCCAAACATGGCGTCTGCTTCGGCTTCGAT
CCCGACAGCTACTTTGGCGCCATGGTGCGACTAAACGGTGAAATTAAGCGCAGCCCGGTGGGGGATTTTCTCGCGAAGCA
CTATGGCCAAACGGTCAGCCGCGCCGACTTCGACGCCGCCGTGGCCCGCGCCTGGGGTCCGCAGAGCGTGAAGGCGTTTA
AGCTAACCTGCAACGGTAATCCGGCCTATCTGACGGAGATGCAAATCTCGCTCAACGCCGCGACCATCAACGCCCCTCTG
GCCACTTCCGCTTTTCTGCCCCAGCCTCACCCGGGTAACTGCGGCGCACAGTTTATCCTCGATAAGGTTGGCCACTAG

Upstream 100 bases:

>100_bases
TCCACTAATGGTCTAACCACGCGAAAAGGCGCCAGCGGGCGCCTTTTTTATGCTTTAATAACTCCCCGTTATCTTTTGCC
ATCCATAATCAGGAATAAAA

Downstream 100 bases:

>100_bases
CGCAAAGCGGGATGCTGATTGGAAAACACCGTCCGCCGGATGGTGTTGCACGCTGAATGTGCGTTTCATCACTTCAAACT
GCCGGCAACTCTCAGTATGA

Product: ribonuclease I

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MFRKDFAAIALVLTATQAGAEPLTATRYADFDRYVLALSWQTGFCQSMYDRNRNEPEECRLQQDTANKADFLTVHGLWPG
LPKSIAARGVDERRWMRYGCATRPVPNMPEVKANRKCQAAETGLSLEMANKLNNVMPGSGGTSCLERYEYAKHGVCFGFD
PDSYFGAMVRLNGEIKRSPVGDFLAKHYGQTVSRADFDAAVARAWGPQSVKAFKLTCNGNPAYLTEMQISLNAATINAPL
ATSAFLPQPHPGNCGAQFILDKVGH

Sequences:

>Translated_265_residues
MFRKDFAAIALVLTATQAGAEPLTATRYADFDRYVLALSWQTGFCQSMYDRNRNEPEECRLQQDTANKADFLTVHGLWPG
LPKSIAARGVDERRWMRYGCATRPVPNMPEVKANRKCQAAETGLSLEMANKLNNVMPGSGGTSCLERYEYAKHGVCFGFD
PDSYFGAMVRLNGEIKRSPVGDFLAKHYGQTVSRADFDAAVARAWGPQSVKAFKLTCNGNPAYLTEMQISLNAATINAPL
ATSAFLPQPHPGNCGAQFILDKVGH
>Mature_265_residues
MFRKDFAAIALVLTATQAGAEPLTATRYADFDRYVLALSWQTGFCQSMYDRNRNEPEECRLQQDTANKADFLTVHGLWPG
LPKSIAARGVDERRWMRYGCATRPVPNMPEVKANRKCQAAETGLSLEMANKLNNVMPGSGGTSCLERYEYAKHGVCFGFD
PDSYFGAMVRLNGEIKRSPVGDFLAKHYGQTVSRADFDAAVARAWGPQSVKAFKLTCNGNPAYLTEMQISLNAATINAPL
ATSAFLPQPHPGNCGAQFILDKVGH

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=265, Percent_Identity=70.188679245283, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 29124; Mature: 29124

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKDFAAIALVLTATQAGAEPLTATRYADFDRYVLALSWQTGFCQSMYDRNRNEPEECR
CCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHH
LQQDTANKADFLTVHGLWPGLPKSIAARGVDERRWMRYGCATRPVPNMPEVKANRKCQAA
CCCCCCCCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
ETGLSLEMANKLNNVMPGSGGTSCLERYEYAKHGVCFGFDPDSYFGAMVRLNGEIKRSPV
HCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEECCCHHHHEEEEEECCCCCCCCH
GDFLAKHYGQTVSRADFDAAVARAWGPQSVKAFKLTCNGNPAYLTEMQISLNAATINAPL
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEEEEEEEEECCCC
ATSAFLPQPHPGNCGAQFILDKVGH
CCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MFRKDFAAIALVLTATQAGAEPLTATRYADFDRYVLALSWQTGFCQSMYDRNRNEPEECR
CCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHH
LQQDTANKADFLTVHGLWPGLPKSIAARGVDERRWMRYGCATRPVPNMPEVKANRKCQAA
CCCCCCCCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
ETGLSLEMANKLNNVMPGSGGTSCLERYEYAKHGVCFGFDPDSYFGAMVRLNGEIKRSPV
HCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEECCCHHHHEEEEEECCCCCCCCH
GDFLAKHYGQTVSRADFDAAVARAWGPQSVKAFKLTCNGNPAYLTEMQISLNAATINAPL
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEEEEEEEEECCCC
ATSAFLPQPHPGNCGAQFILDKVGH
CCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]