Click here to switch to the map view.

The map label for this gene is scrA

Identifier: 238893528

GI number: 238893528

Start: 1375896

End: 1377266

Strand: Reverse

Name: scrA

Synonym: KP1_1431

Alternate gene names: 238893528

Gene position: 1377266-1375896 (Counterclockwise)

Preceding gene: 238893529

Following gene: 238893527

Centisome position: 26.24

GC content: 61.49

Gene sequence:

>1371_bases
ATGGATTTTGAACAGATTTCCCGCTCACTGCTTCCCCTGCTGGGCGGCAAGGAAAACATCGCCAGCGCCGCGCACTGCGC
CACCCGCCTGCGGCTGGTGCTGGTCGACGACGCGCTCGCCGATCAGCAGGCGATTGGCAAAATCGACGGGGTGAAAGGCT
GCTTTCGCAATGCCGGACAGATGCAGATCATCTTCGGCACCGGGGTGGTCAATAAAGTCTATGCCGCCTTTATCCAGGCC
GCAGGCATCAGCGAATCGAGCAAATCCGAAGCCGCCGACCTGGCGGCGAAAAAGCTGAACCCGTTCCAGCGCATCGCCCG
CCTGCTGTCCAACATCTTCGTGCCGATTATTCCGGCCATCGTCGCCTCCGGCCTGCTGATGGGCCTGCTGGGGATGGTGA
AAACCTACGGTTGGGTCGACCCGAGCAACGCTCTCTATATCATGCTGGATATGTGCAGTTCGGCGGCGTTTATCATTCTG
CCGATCCTGATCGGCTTTACCGCCGCCCGCGAATTTGGCGGTAACCCCTATCTGGGCGCGACCCTCGGCGGGATCCTCAC
CCACCCGGCGCTGACCAACGCCTGGGGCGTCGCCGCCGGCTTCCACACCATGAATTTCTTCGGCATCGAAGTGGCGATGA
TCGGCTACCAGGGCACCGTCTTCCCGGTGCTGCTGGCGGTGTGGTTTATGAGCATGGTCGAGAAGCGGCTGCGCCGCGTG
ATCCCTGACGCGCTGGACCTGATCCTCACTCCGTTCCTGACGGTGATTATCTCCGGCTTTATCGCCCTGCTGCTGATCGG
CCCGGCCGGTCGCGCGCTCGGCGACGGCATTTCGTTTATCCTCAGCACGCTTATCAGCCATGCCGGCTGGCTGGCGGGCC
TGCTGTTCGGCGGCCTCTATTCGGTGATCGTCATTACCGGTATCCATCACAGCTTCCATGCCATCGAGGCCGGACTGCTG
GGCAACCCATCGATTGGCGTCAACTTCCTGCTGCCGATCTGGGCGATGGCCAACGTCGCCCAGGGCGGCGCCTGCTTTGC
GGTGTGGTTTAAAACCAAAGATGCCAAAATAAAAGCCATCACCCTGCCGTCGGCGTTTTCGGCGATGCTGGGGATCACCG
AGGCGGCAATCTTCGGGATTAACCTGCGCTTTGTGAAACCGTTTATCGCCGCGCTGGTGGGCGGTGCCGCCGGCGGCGCC
TGGGTGGTGTCGATGCACGTCTACATGACCGCGGTGGGCCTGACGGCGATCCCGGGGATGGCTATCGTGCAGGCCAGCTC
GCTGCTGAACTACATTATCGGAATGGCGATCGCCTTCGCCGTGGCCTTCGCGCTCTCTCTGACGCTGAAATACAAAACGG
ACGCTGAATAA

Upstream 100 bases:

>100_bases
AACCGGGGCGACAGGGTAAATAACACATAAATATAAGGTTCGCGGCGCCTGCCACGGCTGGCGCCGCCCACGCCATATCA
TCATGCATTTAGAGGGTACT

Downstream 100 bases:

>100_bases
TGTCATTACCGTCACGCCTGCCTGCGATCCTGCAGGCCGTTATGCAGGGCCAGCCGCAGGCGCTGGCCGACAGCCATTAT
CCGCAATGGCATCTGGCGCC

Product: sucrose-specific enzyme II

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDFEQISRSLLPLLGGKENIASAAHCATRLRLVLVDDALADQQAIGKIDGVKGCFRNAGQMQIIFGTGVVNKVYAAFIQA
AGISESSKSEAADLAAKKLNPFQRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVDPSNALYIMLDMCSSAAFIIL
PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFHTMNFFGIEVAMIGYQGTVFPVLLAVWFMSMVEKRLRRV
IPDALDLILTPFLTVIISGFIALLLIGPAGRALGDGISFILSTLISHAGWLAGLLFGGLYSVIVITGIHHSFHAIEAGLL
GNPSIGVNFLLPIWAMANVAQGGACFAVWFKTKDAKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAALVGGAAGGA
WVVSMHVYMTAVGLTAIPGMAIVQASSLLNYIIGMAIAFAVAFALSLTLKYKTDAE

Sequences:

>Translated_456_residues
MDFEQISRSLLPLLGGKENIASAAHCATRLRLVLVDDALADQQAIGKIDGVKGCFRNAGQMQIIFGTGVVNKVYAAFIQA
AGISESSKSEAADLAAKKLNPFQRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVDPSNALYIMLDMCSSAAFIIL
PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFHTMNFFGIEVAMIGYQGTVFPVLLAVWFMSMVEKRLRRV
IPDALDLILTPFLTVIISGFIALLLIGPAGRALGDGISFILSTLISHAGWLAGLLFGGLYSVIVITGIHHSFHAIEAGLL
GNPSIGVNFLLPIWAMANVAQGGACFAVWFKTKDAKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAALVGGAAGGA
WVVSMHVYMTAVGLTAIPGMAIVQASSLLNYIIGMAIAFAVAFALSLTLKYKTDAE
>Mature_456_residues
MDFEQISRSLLPLLGGKENIASAAHCATRLRLVLVDDALADQQAIGKIDGVKGCFRNAGQMQIIFGTGVVNKVYAAFIQA
AGISESSKSEAADLAAKKLNPFQRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVDPSNALYIMLDMCSSAAFIIL
PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFHTMNFFGIEVAMIGYQGTVFPVLLAVWFMSMVEKRLRRV
IPDALDLILTPFLTVIISGFIALLLIGPAGRALGDGISFILSTLISHAGWLAGLLFGGLYSVIVITGIHHSFHAIEAGLL
GNPSIGVNFLLPIWAMANVAQGGACFAVWFKTKDAKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAALVGGAAGGA
WVVSMHVYMTAVGLTAIPGMAIVQASSLLNYIIGMAIAFAVAFALSLTLKYKTDAE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain

Homologues:

Organism=Escherichia coli, GI2367362, Length=429, Percent_Identity=34.2657342657343, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790159, Length=457, Percent_Identity=29.9781181619256, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI48994906, Length=410, Percent_Identity=30.7317073170732, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788769, Length=469, Percent_Identity=31.7697228144989, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTSBC_KLEPN (P27219)

Other databases:

- EMBL:   X57401
- PIR:   S62331
- ProteinModelPortal:   P27219
- SMR:   P27219
- BioCyc:   MetaCyc:MONOMER-12625
- BRENDA:   2.7.1.69
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973
- Gene3D:   G3DSA:3.30.1360.60
- TIGRFAMs:   TIGR00826
- TIGRFAMs:   TIGR00852
- TIGRFAMs:   TIGR01996

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC; SSF55604 PTS_EIIB

EC number: =2.7.1.69

Molecular weight: Translated: 48023; Mature: 48023

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS51098 PTS_EIIB_TYPE_1; PS01035 PTS_EIIB_TYPE_1_CYS; PS51103 PTS_EIIC_TYPE_1

Important sites: ACT_SITE 26-26

Signals:

None

Transmembrane regions:

HASH(0x19ee8c94)-; HASH(0x1ac3aad8)-; HASH(0x196bbd6c)-; HASH(0x1a9a1874)-; HASH(0x1a417dd8)-; HASH(0x1aaf4eac)-; HASH(0x1a5e91c0)-; HASH(0x1a527adc)-; HASH(0x19bfb37c)-; HASH(0x1ad1a6ac)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFEQISRSLLPLLGGKENIASAAHCATRLRLVLVDDALADQQAIGKIDGVKGCFRNAGQ
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
MQIIFGTGVVNKVYAAFIQAAGISESSKSEAADLAAKKLNPFQRIARLLSNIFVPIIPAI
EEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMVKTYGWVDPSNALYIMLDMCSSAAFIILPILIGFTAAREFGGNPYLGA
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCCCCHHH
TLGGILTHPALTNAWGVAAGFHTMNFFGIEVAMIGYQGTVFPVLLAVWFMSMVEKRLRRV
HHHHHHHCCCHHCCHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IPDALDLILTPFLTVIISGFIALLLIGPAGRALGDGISFILSTLISHAGWLAGLLFGGLY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVIVITGIHHSFHAIEAGLLGNPSIGVNFLLPIWAMANVAQGGACFAVWFKTKDAKIKAI
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEE
TLPSAFSAMLGITEAAIFGINLRFVKPFIAALVGGAAGGAWVVSMHVYMTAVGLTAIPGM
ECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCH
AIVQASSLLNYIIGMAIAFAVAFALSLTLKYKTDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCH
>Mature Secondary Structure
MDFEQISRSLLPLLGGKENIASAAHCATRLRLVLVDDALADQQAIGKIDGVKGCFRNAGQ
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
MQIIFGTGVVNKVYAAFIQAAGISESSKSEAADLAAKKLNPFQRIARLLSNIFVPIIPAI
EEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMVKTYGWVDPSNALYIMLDMCSSAAFIILPILIGFTAAREFGGNPYLGA
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCCCCHHH
TLGGILTHPALTNAWGVAAGFHTMNFFGIEVAMIGYQGTVFPVLLAVWFMSMVEKRLRRV
HHHHHHHCCCHHCCHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IPDALDLILTPFLTVIISGFIALLLIGPAGRALGDGISFILSTLISHAGWLAGLLFGGLY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVIVITGIHHSFHAIEAGLLGNPSIGVNFLLPIWAMANVAQGGACFAVWFKTKDAKIKAI
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEE
TLPSAFSAMLGITEAAIFGINLRFVKPFIAALVGGAAGGAWVVSMHVYMTAVGLTAIPGM
ECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCH
AIVQASSLLNYIIGMAIAFAVAFALSLTLKYKTDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8628219