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The map label for this gene is ddl [H]

Identifier: 238893305

GI number: 238893305

Start: 1147874

End: 1148971

Strand: Reverse

Name: ddl [H]

Synonym: KP1_1182

Alternate gene names: 238893305

Gene position: 1148971-1147874 (Counterclockwise)

Preceding gene: 238893311

Following gene: 238893303

Centisome position: 21.89

GC content: 59.65

Gene sequence:

>1098_bases
ATGGCAAAGATGAGGGTTGGGATCGTTTTTGGCGGCAAGTCGGCGGAGCATGAAGTGTCGCTGCAGTCAGCGAAGAATAT
CGTGGAAGCGATTGATAAATCGCGCTTCGATGTGGTTCTGTTGGGTATTGATAAACAGGGGCTGTGGCACATCAACGACG
CCGGCAACTACCTGCTCAACGCACAGGATCCTGCCCGCATTGCCCTGCGCCCTTCCACGGTGACTCTGGCGCAGATCCCG
GGCCGCGAAGCGCAGCAGCTGATTAACGCCGAAAGCGGCCAACCGCTGGCGGCCATCGATGTGATTTTCCCGATCGTCCA
CGGCACGCTGGGTGAAGATGGCTCCCTGCAGGGGATGCTGCGCATGGCGAATCTGCCGTTCGTCGGCTCCGATGTGCTGG
GCTCTGCGGCCTGTATGGACAAAGACGTGACCAAACGTCTGCTGCGCGACGCCGGGCTCGCCGTCGCCCCGTTTATCACA
CTTACCCGCGCCAACCGCGCGCAGTTCAGCTTTGCCGATGTCGAAGCGAAGCTTGGCCTGCCGCTGTTCGTTAAGCCGGC
TAATCAGGGCTCGTCGGTTGGCGTCAGCAAAGTTAAAAACGAAGAGCAGTACCATCAGGCGGTGGCCCTGGCCTTCGAAT
TTGACCATAAAGTGGTGGTGGAGCAAGGCATTAAAGGGCGGGAAATTGAGTGTGCGGTGCTGGGTAACGACCACCCGCAG
GCCAGCACCTGCGGTGAAATCGTCCTCAACAGCGAGTTTTACGCCTACGACACCAAGTATATCGACGATCAGGGCGCGCA
GGTGGTAGTCCCGGCCGCTATCGCCCCTGAGATCAACGACAAAATTCGCGCTATCGCGGTGCAGGCCTACCAGACCCTGG
GCTGCAGCGGCATGGCTCGTGTCGACGTGTTCCTCACCGCCGACAACGAGGTGGTGATCAACGAGATCAACACCCTGCCG
GGCTTTACCAACATCAGCATGTATCCGAAGCTGTGGCAGGCCAGCGGTCTCGACTACACCAGCCTGATCACCCGCCTGAT
TGAGCTGGCGCTGGAGCGCCATGCCGCGGACCGGGCGCTGAAAACCTCCATGAACTAA

Upstream 100 bases:

>100_bases
CGCCCACTCCCGTCAATTGATCAGGTAAATATTATGGCAGCCAGGCAGCGGTTTCACATGGTATGTTATGCCCTTTGATG
TTATCTGGATGGAAGTTGAA

Downstream 100 bases:

>100_bases
AACGCGTTTTACCCCGCCTGGCAGCGGCGGCCTCAGGCCTCTTCTGCCGGGCGGCGGCGGATCACAAAGCCCGCAATCCA
GAAGCTAATGACCCAGGTGA

Product: D-alanylalanine synthetase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MAKMRVGIVFGGKSAEHEVSLQSAKNIVEAIDKSRFDVVLLGIDKQGLWHINDAGNYLLNAQDPARIALRPSTVTLAQIP
GREAQQLINAESGQPLAAIDVIFPIVHGTLGEDGSLQGMLRMANLPFVGSDVLGSAACMDKDVTKRLLRDAGLAVAPFIT
LTRANRAQFSFADVEAKLGLPLFVKPANQGSSVGVSKVKNEEQYHQAVALAFEFDHKVVVEQGIKGREIECAVLGNDHPQ
ASTCGEIVLNSEFYAYDTKYIDDQGAQVVVPAAIAPEINDKIRAIAVQAYQTLGCSGMARVDVFLTADNEVVINEINTLP
GFTNISMYPKLWQASGLDYTSLITRLIELALERHAADRALKTSMN

Sequences:

>Translated_365_residues
MAKMRVGIVFGGKSAEHEVSLQSAKNIVEAIDKSRFDVVLLGIDKQGLWHINDAGNYLLNAQDPARIALRPSTVTLAQIP
GREAQQLINAESGQPLAAIDVIFPIVHGTLGEDGSLQGMLRMANLPFVGSDVLGSAACMDKDVTKRLLRDAGLAVAPFIT
LTRANRAQFSFADVEAKLGLPLFVKPANQGSSVGVSKVKNEEQYHQAVALAFEFDHKVVVEQGIKGREIECAVLGNDHPQ
ASTCGEIVLNSEFYAYDTKYIDDQGAQVVVPAAIAPEINDKIRAIAVQAYQTLGCSGMARVDVFLTADNEVVINEINTLP
GFTNISMYPKLWQASGLDYTSLITRLIELALERHAADRALKTSMN
>Mature_364_residues
AKMRVGIVFGGKSAEHEVSLQSAKNIVEAIDKSRFDVVLLGIDKQGLWHINDAGNYLLNAQDPARIALRPSTVTLAQIPG
REAQQLINAESGQPLAAIDVIFPIVHGTLGEDGSLQGMLRMANLPFVGSDVLGSAACMDKDVTKRLLRDAGLAVAPFITL
TRANRAQFSFADVEAKLGLPLFVKPANQGSSVGVSKVKNEEQYHQAVALAFEFDHKVVVEQGIKGREIECAVLGNDHPQA
STCGEIVLNSEFYAYDTKYIDDQGAQVVVPAAIAPEINDKIRAIAVQAYQTLGCSGMARVDVFLTADNEVVINEINTLPG
FTNISMYPKLWQASGLDYTSLITRLIELALERHAADRALKTSMN

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=364, Percent_Identity=85.989010989011, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1786280, Length=351, Percent_Identity=33.6182336182336, Blast_Score=188, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39446; Mature: 39314

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRVGIVFGGKSAEHEVSLQSAKNIVEAIDKSRFDVVLLGIDKQGLWHINDAGNYLLN
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCEEEE
AQDPARIALRPSTVTLAQIPGREAQQLINAESGQPLAAIDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCEEEEEECCCHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHH
RMANLPFVGSDVLGSAACMDKDVTKRLLRDAGLAVAPFITLTRANRAQFSFADVEAKLGL
EECCCCEECHHHHCHHHHCCHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEECCHHHCCC
PLFVKPANQGSSVGVSKVKNEEQYHQAVALAFEFDHKVVVEQGIKGREIECAVLGNDHPQ
EEEEEECCCCCCCCHHHHCCHHHHHHHEEEEEECCCEEEECCCCCCCEEEEEEECCCCCC
ASTCGEIVLNSEFYAYDTKYIDDQGAQVVVPAAIAPEINDKIRAIAVQAYQTLGCSGMAR
CCCCCCEEECCEEEEEECEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEE
VDVFLTADNEVVINEINTLPGFTNISMYPKLWQASGLDYTSLITRLIELALERHAADRAL
EEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
KTSMN
HHCCC
>Mature Secondary Structure 
AKMRVGIVFGGKSAEHEVSLQSAKNIVEAIDKSRFDVVLLGIDKQGLWHINDAGNYLLN
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCEEEE
AQDPARIALRPSTVTLAQIPGREAQQLINAESGQPLAAIDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCEEEEEECCCHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHH
RMANLPFVGSDVLGSAACMDKDVTKRLLRDAGLAVAPFITLTRANRAQFSFADVEAKLGL
EECCCCEECHHHHCHHHHCCHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEECCHHHCCC
PLFVKPANQGSSVGVSKVKNEEQYHQAVALAFEFDHKVVVEQGIKGREIECAVLGNDHPQ
EEEEEECCCCCCCCHHHHCCHHHHHHHEEEEEECCCEEEECCCCCCCEEEEEEECCCCCC
ASTCGEIVLNSEFYAYDTKYIDDQGAQVVVPAAIAPEINDKIRAIAVQAYQTLGCSGMAR
CCCCCCEEECCEEEEEECEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEE
VDVFLTADNEVVINEINTLPGFTNISMYPKLWQASGLDYTSLITRLIELALERHAADRAL
EEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
KTSMN
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]