Click here to switch to the map view.

The map label for this gene is livK [C]

Identifier: 238893286

GI number: 238893286

Start: 1127829

End: 1128965

Strand: Reverse

Name: livK [C]

Synonym: KP1_1161

Alternate gene names: 238893286

Gene position: 1128965-1127829 (Counterclockwise)

Preceding gene: 238893287

Following gene: 238893285

Centisome position: 21.51

GC content: 58.49

Gene sequence:

>1137_bases
ATGAAATCGTTTTCCGGGAAAGCGTCTTTAATGGCCACGCTGGTTGCCGCGCTGGTCGGCGCCAGCAGCGCGCAGGCAGC
GGAAATTAAAATCGGGGTGGTATTACCCCTGAGCGGCGCCCTCAGCGGCTATGGCCAGCCGTCGCAGAAGGGGCTGGACA
TTATTCAGGCCATCACCCCAACATTAAAAAATGGCGATACCATCAAGCTTATCGTTATCGATGATAAAAGCGACAAAGTC
GAAGCGGCCAACGCCATGCAGCGCCTGGTCTCCAGCGATAAAGTGGATGCCGTGATCGGGGAAGTCACCTCCTCCAACAC
CCTGGCGATGACCAAAATCGCCGACGACAGCAAAACGCCGCTGGTCTCCTCCACCGCCACCAACGACCGCGTCACCCGCA
ACCATCCCTACGTCAGCCGGGTCTGCTTCTCGGACAGCTTCCAGGGGGTGGTCGGGGCCAATCTGGCCTCCCGCGATCTG
AAAGCCAAAACCGCCGCCATCGTGTTTGACAGCAGCAACGACTACTCCGTCGGCCTGGCGAAAGCGTTCCGCACCCAGTT
CCTGAAAAACGGCGGCACCATTCCCATCGAGGTCCAGGCCCCGGGCGGCAGCAAGGACTTTAAAGCCCAGCTGGCGAGCG
TCAAGGCGAAGAATGTCGACATGATTTACATGCCGATCTACTACACCGAAGGGGCGCTGATTGCCGTGCAGTCTAAACAG
CTGGGGCTCAACAAACCGGTGGTGGGCGGCGACGGTCTGGCCGCGGATCAGGTCTTCTTCGATGTCGGTAAAGACGCGGT
CAACGGCTATATGACCACCGATTATTACTCGCCGAACGCCAAAGAGCAGACCCCGGCGGGGGAAACCTTCATCAAAGCCT
GGGAAGCCAAATACCAGCAGCCGACCCATACCTGGGGCGCAATGGCGGCGGACGCCTATAACGTCATCGTCAACGCCATG
AACCAGTGCAGCGATCCCCACGATCGGGTCTGCGTAAATGAAAAAATCCGCGCCACCAAAGATTTCCAGGGCGTGACCGG
CACGCTGACGTTACAGAACGGCGACGCCATTCGCAGCGCGGTCATCAACGAAGTGAAAGATGGCAAGCTGGCGTTTCGTA
CCGTGGTTAACCCTTAA

Upstream 100 bases:

>100_bases
TAACAGCCAATAATTCGCCGGATACGCTGGCGCATTATTCCTCAATGTTATGCAGTCATTGATGGTTAAAGATAAATAAT
CGACCGACGGAGAAATACCA

Downstream 100 bases:

>100_bases
GTTGTGACGAGGCTACAAAATGGACGGCGCCATTTTTCTCCAGCAAGTGGTCAATGGAATGAGTCTTGGGGGCATGTACG
CCCTGATTGCCATCGGTTAT

Product: ABC transport system periplasmic binding component

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKSFSGKASLMATLVAALVGASSAQAAEIKIGVVLPLSGALSGYGQPSQKGLDIIQAITPTLKNGDTIKLIVIDDKSDKV
EAANAMQRLVSSDKVDAVIGEVTSSNTLAMTKIADDSKTPLVSSTATNDRVTRNHPYVSRVCFSDSFQGVVGANLASRDL
KAKTAAIVFDSSNDYSVGLAKAFRTQFLKNGGTIPIEVQAPGGSKDFKAQLASVKAKNVDMIYMPIYYTEGALIAVQSKQ
LGLNKPVVGGDGLAADQVFFDVGKDAVNGYMTTDYYSPNAKEQTPAGETFIKAWEAKYQQPTHTWGAMAADAYNVIVNAM
NQCSDPHDRVCVNEKIRATKDFQGVTGTLTLQNGDAIRSAVINEVKDGKLAFRTVVNP

Sequences:

>Translated_378_residues
MKSFSGKASLMATLVAALVGASSAQAAEIKIGVVLPLSGALSGYGQPSQKGLDIIQAITPTLKNGDTIKLIVIDDKSDKV
EAANAMQRLVSSDKVDAVIGEVTSSNTLAMTKIADDSKTPLVSSTATNDRVTRNHPYVSRVCFSDSFQGVVGANLASRDL
KAKTAAIVFDSSNDYSVGLAKAFRTQFLKNGGTIPIEVQAPGGSKDFKAQLASVKAKNVDMIYMPIYYTEGALIAVQSKQ
LGLNKPVVGGDGLAADQVFFDVGKDAVNGYMTTDYYSPNAKEQTPAGETFIKAWEAKYQQPTHTWGAMAADAYNVIVNAM
NQCSDPHDRVCVNEKIRATKDFQGVTGTLTLQNGDAIRSAVINEVKDGKLAFRTVVNP
>Mature_378_residues
MKSFSGKASLMATLVAALVGASSAQAAEIKIGVVLPLSGALSGYGQPSQKGLDIIQAITPTLKNGDTIKLIVIDDKSDKV
EAANAMQRLVSSDKVDAVIGEVTSSNTLAMTKIADDSKTPLVSSTATNDRVTRNHPYVSRVCFSDSFQGVVGANLASRDL
KAKTAAIVFDSSNDYSVGLAKAFRTQFLKNGGTIPIEVQAPGGSKDFKAQLASVKAKNVDMIYMPIYYTEGALIAVQSKQ
LGLNKPVVGGDGLAADQVFFDVGKDAVNGYMTTDYYSPNAKEQTPAGETFIKAWEAKYQQPTHTWGAMAADAYNVIVNAM
NQCSDPHDRVCVNEKIRATKDFQGVTGTLTLQNGDAIRSAVINEVKDGKLAFRTVVNP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=329, Percent_Identity=27.0516717325228, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789867, Length=308, Percent_Identity=26.9480519480519, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40104; Mature: 40104

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFSGKASLMATLVAALVGASSAQAAEIKIGVVLPLSGALSGYGQPSQKGLDIIQAITP
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCC
TLKNGDTIKLIVIDDKSDKVEAANAMQRLVSSDKVDAVIGEVTSSNTLAMTKIADDSKTP
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEECCCCCCC
LVSSTATNDRVTRNHPYVSRVCFSDSFQGVVGANLASRDLKAKTAAIVFDSSNDYSVGLA
EEECCCCCCCEECCCCCCEEEECCCCCCCHHCCCCHHCCCCCEEEEEEEECCCCCCHHHH
KAFRTQFLKNGGTIPIEVQAPGGSKDFKAQLASVKAKNVDMIYMPIYYTEGALIAVQSKQ
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEECCC
LGLNKPVVGGDGLAADQVFFDVGKDAVNGYMTTDYYSPNAKEQTPAGETFIKAWEAKYQQ
CCCCCCEECCCCCCHHHHHHHCCHHHHCCEEEECEECCCCCCCCCCCHHHHHHHHHHCCC
PTHTWGAMAADAYNVIVNAMNQCSDPHDRVCVNEKIRATKDFQGVTGTLTLQNGDAIRSA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHCCCCCCCCEEEEEECCCHHHHHH
VINEVKDGKLAFRTVVNP
HHHHCCCCCEEEEEECCC
>Mature Secondary Structure
MKSFSGKASLMATLVAALVGASSAQAAEIKIGVVLPLSGALSGYGQPSQKGLDIIQAITP
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCC
TLKNGDTIKLIVIDDKSDKVEAANAMQRLVSSDKVDAVIGEVTSSNTLAMTKIADDSKTP
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEECCCCCCC
LVSSTATNDRVTRNHPYVSRVCFSDSFQGVVGANLASRDLKAKTAAIVFDSSNDYSVGLA
EEECCCCCCCEECCCCCCEEEECCCCCCCHHCCCCHHCCCCCEEEEEEEECCCCCCHHHH
KAFRTQFLKNGGTIPIEVQAPGGSKDFKAQLASVKAKNVDMIYMPIYYTEGALIAVQSKQ
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEECCC
LGLNKPVVGGDGLAADQVFFDVGKDAVNGYMTTDYYSPNAKEQTPAGETFIKAWEAKYQQ
CCCCCCEECCCCCCHHHHHHHCCHHHHCCEEEECEECCCCCCCCCCCHHHHHHHHHHCCC
PTHTWGAMAADAYNVIVNAMNQCSDPHDRVCVNEKIRATKDFQGVTGTLTLQNGDAIRSA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHCCCCCCCCEEEEEECCCHHHHHH
VINEVKDGKLAFRTVVNP
HHHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]