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The map label for this gene is tilS [H]

Identifier: 238893184

GI number: 238893184

Start: 1024759

End: 1026060

Strand: Direct

Name: tilS [H]

Synonym: KP1_1046

Alternate gene names: 238893184

Gene position: 1024759-1026060 (Clockwise)

Preceding gene: 238893183

Following gene: 238893187

Centisome position: 19.52

GC content: 65.67

Gene sequence:

>1302_bases
ATGACGGCTGATATTGCGCATACGCTTCATCCCCATCGCCAGCTGCTGGTGGCCTTCAGCGGCGGTCTGGACTCGACGGT
TCTTCTGCATCAGCTGGTGCTGCTGCGGGAACAGGATCCCTCGCTGACGCTGCGGGCGGTGCATGTTCACCACGGCCTTA
GCGCCCACGCAGACGACTGGGTGGCGCATTGTCGGCAGATTTGCCAGCAGTGGCAGGTGCCGCTGGTGGTGCATCACGTG
ACGTTAGCCCGGGGAGGGCTGGGCGTCGAGGCCCACGCTCGTGCGGCGCGCTATCAGGCCTTTCAGGATACGCTCAATGC
CGGCGAGGTGTTAGTGACCGCCCAGCACCAGGACGATCAGTGCGAAACCCTGCTGCTGGCGTTAAAACGCGGCAGCGGTC
CTACCGGCCTGTCGGCGATGGCGCCATCCTCTGCCTTTGCCGGCAGTCGACTACTGCGTCCTCTCCTCAATGAAACCCGC
GAATCACTACGCCAGTGGGCGCTCGCCCATCAGCTGTCGTGGATCGAAGATGAGAGTAATCAGGATGATACCTATGACCG
CAATTTCCTGCGCCTGCGGGTGATCCCGGTGCTGCGCGAGCGTTGGCCGCACTTTAGCGAGGCCGTCGCCCGCAGCGCCA
GCCTGTGCGCCGAGCAGGAACAACTGCTGGACGAGATGTTGGCGGCGGAGCTGGCGTCGCTGGTGGCCGAAGATGGCTCG
CTGGCTATCGCGCCGCTGGCGTCGATGAGTCCGCCGCGCCGAGCGGCGCTGCTGCGTCGCTGGCTCGCCAGGCAGCAGGC
GCCGATGCCCGCCCGCGAGGTGCCTGAGCGCCTCTGGCACGAGGTGGCGCTGGCGCGGGAAGATGCCAGCCCCTGTCTGC
GGCTGGGGGAATTTACCGTCCGCCGTTTTCAACAGCGTTTGTACTGGGTGAAGTATCTTCCCGGTCAGACGGACAGCGTT
CAGCGCTGGCCCGACTGGCGCCAGCCGCTGCGGCTGGCGGACGGTCTCGGCGAGCTGACGCTGCAGCCCGGCGGCAGGCT
GCGCCCGCCGTCGGCGGATGAGCCGGTCACCGTCCGCTTTCGCGCCAGCGGCCACCTGCATATTGTCGGGCGCCATGGCG
GACGCAAGCTTAAGAAGCTCTGGCAGGAGCTGGGCGTTGCTCCGTGGCGACGAGACACCACGCCGCTGCTGTTTTACGGC
GAAACGCCGATTGCCGCAGCGGACGATCTGTTTGTGACCACAGAAGGTGAAGTGAAGGACGGGGAAGGGGTCAGGCTGAT
GTGGAGGAAAACGGGCGATTAA

Upstream 100 bases:

>100_bases
CGGACGACCTGCCGCTGGAAATATATCAGCAGTAAATCTTGCCAGCGCGGCCCTGGCCCGGTAACGTGCCGGGCCATCAC
TTCTCGGTATGTTCGTGGAT

Downstream 100 bases:

>100_bases
GCCCGTTTTTGATTCAGGATTCGCTCACCACCACGGTGCCGATTTCCGGGTGACTGAAGCTGGCGATTTTATCCAGGCGC
AGCTCGCGGACGTTGCCGGC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVLLREQDPSLTLRAVHVHHGLSAHADDWVAHCRQICQQWQVPLVVHHV
TLARGGLGVEAHARAARYQAFQDTLNAGEVLVTAQHQDDQCETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLLNETR
ESLRQWALAHQLSWIEDESNQDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVAEDGS
LAIAPLASMSPPRRAALLRRWLARQQAPMPAREVPERLWHEVALAREDASPCLRLGEFTVRRFQQRLYWVKYLPGQTDSV
QRWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPVTVRFRASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPLLFYG
ETPIAAADDLFVTTEGEVKDGEGVRLMWRKTGD

Sequences:

>Translated_433_residues
MTADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVLLREQDPSLTLRAVHVHHGLSAHADDWVAHCRQICQQWQVPLVVHHV
TLARGGLGVEAHARAARYQAFQDTLNAGEVLVTAQHQDDQCETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLLNETR
ESLRQWALAHQLSWIEDESNQDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVAEDGS
LAIAPLASMSPPRRAALLRRWLARQQAPMPAREVPERLWHEVALAREDASPCLRLGEFTVRRFQQRLYWVKYLPGQTDSV
QRWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPVTVRFRASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPLLFYG
ETPIAAADDLFVTTEGEVKDGEGVRLMWRKTGD
>Mature_432_residues
TADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVLLREQDPSLTLRAVHVHHGLSAHADDWVAHCRQICQQWQVPLVVHHVT
LARGGLGVEAHARAARYQAFQDTLNAGEVLVTAQHQDDQCETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLLNETRE
SLRQWALAHQLSWIEDESNQDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVAEDGSL
AIAPLASMSPPRRAALLRRWLARQQAPMPAREVPERLWHEVALAREDASPCLRLGEFTVRRFQQRLYWVKYLPGQTDSVQ
RWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPVTVRFRASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPLLFYGE
TPIAAADDLFVTTEGEVKDGEGVRLMWRKTGD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=431, Percent_Identity=65.8932714617169, Blast_Score=555, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 48644; Mature: 48513

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVLLREQDPSLTLRAVHVHHGLSAHADDW
CCCCHHHHHCHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
VAHCRQICQQWQVPLVVHHVTLARGGLGVEAHARAARYQAFQDTLNAGEVLVTAQHQDDQ
HHHHHHHHHHCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
CETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLLNETRESLRQWALAHQLSWIEDESN
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVAEDGS
CCCCCCCCEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LAIAPLASMSPPRRAALLRRWLARQQAPMPAREVPERLWHEVALAREDASPCLRLGEFTV
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RRFQQRLYWVKYLPGQTDSVQRWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPVTVRF
HHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEE
RASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPLLFYGETPIAAADDLFVTTEGEVKD
ECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCC
GEGVRLMWRKTGD
CCCEEEEEEECCC
>Mature Secondary Structure 
TADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVLLREQDPSLTLRAVHVHHGLSAHADDW
CCCHHHHHCHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
VAHCRQICQQWQVPLVVHHVTLARGGLGVEAHARAARYQAFQDTLNAGEVLVTAQHQDDQ
HHHHHHHHHHCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
CETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLLNETRESLRQWALAHQLSWIEDESN
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVAEDGS
CCCCCCCCEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LAIAPLASMSPPRRAALLRRWLARQQAPMPAREVPERLWHEVALAREDASPCLRLGEFTV
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RRFQQRLYWVKYLPGQTDSVQRWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPVTVRF
HHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEE
RASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPLLFYGETPIAAADDLFVTTEGEVKD
ECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCC
GEGVRLMWRKTGD
CCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA