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The map label for this gene is pulS [H]

Identifier: 238893145

GI number: 238893145

Start: 977251

End: 977625

Strand: Reverse

Name: pulS [H]

Synonym: KP1_0998

Alternate gene names: 238893145

Gene position: 977625-977251 (Counterclockwise)

Preceding gene: 238893152

Following gene: 238893140

Centisome position: 18.63

GC content: 54.4

Gene sequence:

>375_bases
ATGCGTATTCCGCTTATATTTCCGCTTTGCATGGTAGCCCTTCTCTCTGGCTGCCAACAAAAACCTGCCTCAACGCTCAG
CCCGGCCATCAGCAGCCAGGCGCAGCTCGAGCAGCTCTCGTCCGTGGCGGCCGGGACGCGCTATCTCAAGAATAAATGTA
ATCGTAGCGATCTCCCCGCCGATGAAACGATATATCGGGCAGCGGTGAATGTCGGTAAAGCGCGCGGATGGGGAAATATT
GACGTGGCGACGTTAAGCCAAAATAGCGACCGGCTTTACCAACAGCTTTTACAGGACAGTACCCCGGAAGCCACACAGTG
CAGTCAATTTAATCGTCAGCTCGCGCCATTTATTGCCAGCTTGCGCAGCGACTAA

Upstream 100 bases:

>100_bases
AGAGCCTGTTAACATTTATGCAGAATAATATTTTATGATGACACCAGGGAAATATTTGTTAAACTAGCGGCCAGTGATTC
ATTATTTTGATTACTCTATG

Downstream 100 bases:

>100_bases
GCGAAATATCCTCAGCGCGCCGGTTATTGTGCGCGCTTTTATTCCTCTTTCGGCTCCTCGTCAATTTTTCCGCCCGGCCA
GTCCTCCAGCGCCGCGAGGG

Product: pullulanase-specific type II secretion system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MRIPLIFPLCMVALLSGCQQKPASTLSPAISSQAQLEQLSSVAAGTRYLKNKCNRSDLPADETIYRAAVNVGKARGWGNI
DVATLSQNSDRLYQQLLQDSTPEATQCSQFNRQLAPFIASLRSD

Sequences:

>Translated_124_residues
MRIPLIFPLCMVALLSGCQQKPASTLSPAISSQAQLEQLSSVAAGTRYLKNKCNRSDLPADETIYRAAVNVGKARGWGNI
DVATLSQNSDRLYQQLLQDSTPEATQCSQFNRQLAPFIASLRSD
>Mature_124_residues
MRIPLIFPLCMVALLSGCQQKPASTLSPAISSQAQLEQLSSVAAGTRYLKNKCNRSDLPADETIYRAAVNVGKARGWGNI
DVATLSQNSDRLYQQLLQDSTPEATQCSQFNRQLAPFIASLRSD

Specific function: Involved in the secretion of pullulanase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To E.chrysanthemi outS [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005699
- InterPro:   IPR019114 [H]

Pfam domain/function: PF09691 PulS_OutS [H]

EC number: NA

Molecular weight: Translated: 13531; Mature: 13531

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPLIFPLCMVALLSGCQQKPASTLSPAISSQAQLEQLSSVAAGTRYLKNKCNRSDLPA
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DETIYRAAVNVGKARGWGNIDVATLSQNSDRLYQQLLQDSTPEATQCSQFNRQLAPFIAS
HHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LRSD
HHCC
>Mature Secondary Structure
MRIPLIFPLCMVALLSGCQQKPASTLSPAISSQAQLEQLSSVAAGTRYLKNKCNRSDLPA
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DETIYRAAVNVGKARGWGNIDVATLSQNSDRLYQQLLQDSTPEATQCSQFNRQLAPFIAS
HHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LRSD
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2661532 [H]