Click here to switch to the map view.

The map label for this gene is rihC [H]

Identifier: 238893011

GI number: 238893011

Start: 823227

End: 824141

Strand: Direct

Name: rihC [H]

Synonym: KP1_0846

Alternate gene names: 238893011

Gene position: 823227-824141 (Clockwise)

Preceding gene: 238893010

Following gene: 238893012

Centisome position: 15.68

GC content: 62.84

Gene sequence:

>915_bases
ATGCGCTTACCAATCATTCTTGATACCGATCCGGGCATCGACGATGCCGCCGCCATTGCCGCGGCGCTGTTCGCGCCTGA
GCTGGATCTGCAGTTGATGACCACCGTAGCGGGCAATGTCTCGGTGGAGAAGACGACCCGTAACGCGCTGCAATTGCTGC
ATTTCTGGAATGCCGATGTACCGCTGGCGCAGGGCGCGTCGATGCCGCTGGTGCGTCCGTTGCGCGACGCCGCCTCGGTG
CATGGCGAATCCGGCATGGAGGGGTATGACTTTGTTGAGCATCAACGTCAACCGTTGGCGAAGCCGGCCTTCCAGGCTAT
CCGTGATGCGCTTATGCATGCTGCCGAGCCGATCACCCTGGTGGCGATTGGCCCGCTGACCAATATAGCGCTGCTGCTGA
CCCAGTACCCGGAATGCGTGTTTAATATTCGCCGGCTGGTGATCATGGGCGGCTCGGCGGGCCGCGGCAACTTCACGCCG
AATGCGGAATTCAATATCGCTATCGACCCGGAGGCCGCCGCGAAAGTGTTCCACAGCGGGCTGGAGATTGTGATGTGCGG
GCTGGACGTCACCAACCGGGCGCTGCTGGCGGCGGATTATCTGGCAACGCTGCCGACGCTGAACCAGACCGGTAAAATGC
TCCATGCGCTGTTTAGCCACTATCGCAGCGGCAGCATGAGCAGCGGTCTGCGCATGCACGACTTGTGCGCTATTGCGTGG
CTGGCCCGCCCGGAACTGTTCACTCTCCAGCCGTGCTTTGTGGCGGTGGAGACGCAGGGGACCTGGACCGCCGGCACCAC
GGTGGTGGATATCGAAGGACGACTGGGCCAGCCGGCGAACGCCCAGGTGGCGCTGGATATCGACGTCGAAGGGTTTCAGC
GCTGGGCGGCGGAGGTGATTGCCCTGGCGCCGTGA

Upstream 100 bases:

>100_bases
AGAGCTGCGTGTGGATGTCCGTGAAGTAGAGTAAGTTCTTTCCTCCAGCAGTATGAGAAGATGCCAGACTTAACGTCTGG
CATTTTTTTAGGGAGAAACC

Downstream 100 bases:

>100_bases
TATATCACTCGATTTTCAAGGAGAGAAGCAATGACCTATGGACAGGCGTATTTGAGCGGCTGGAAAGAGACCTTTAATTT
TAGTGGCCGCGCCAGCCGGC

Product: ribonucleoside hydrolase RihC

Products: uracil; ribose; xanthine; hypoxanthine; adenine; cytosine; guanine [C]

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRLPIILDTDPGIDDAAAIAAALFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNADVPLAQGASMPLVRPLRDAASV
HGESGMEGYDFVEHQRQPLAKPAFQAIRDALMHAAEPITLVAIGPLTNIALLLTQYPECVFNIRRLVIMGGSAGRGNFTP
NAEFNIAIDPEAAAKVFHSGLEIVMCGLDVTNRALLAADYLATLPTLNQTGKMLHALFSHYRSGSMSSGLRMHDLCAIAW
LARPELFTLQPCFVAVETQGTWTAGTTVVDIEGRLGQPANAQVALDIDVEGFQRWAAEVIALAP

Sequences:

>Translated_304_residues
MRLPIILDTDPGIDDAAAIAAALFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNADVPLAQGASMPLVRPLRDAASV
HGESGMEGYDFVEHQRQPLAKPAFQAIRDALMHAAEPITLVAIGPLTNIALLLTQYPECVFNIRRLVIMGGSAGRGNFTP
NAEFNIAIDPEAAAKVFHSGLEIVMCGLDVTNRALLAADYLATLPTLNQTGKMLHALFSHYRSGSMSSGLRMHDLCAIAW
LARPELFTLQPCFVAVETQGTWTAGTTVVDIEGRLGQPANAQVALDIDVEGFQRWAAEVIALAP
>Mature_304_residues
MRLPIILDTDPGIDDAAAIAAALFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNADVPLAQGASMPLVRPLRDAASV
HGESGMEGYDFVEHQRQPLAKPAFQAIRDALMHAAEPITLVAIGPLTNIALLLTQYPECVFNIRRLVIMGGSAGRGNFTP
NAEFNIAIDPEAAAKVFHSGLEIVMCGLDVTNRALLAADYLATLPTLNQTGKMLHALFSHYRSGSMSSGLRMHDLCAIAW
LARPELFTLQPCFVAVETQGTWTAGTTVVDIEGRLGQPANAQVALDIDVEGFQRWAAEVIALAP

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786213, Length=303, Percent_Identity=83.1683168316832, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1786871, Length=299, Percent_Identity=45.819397993311, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1788486, Length=307, Percent_Identity=38.4364820846906, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI32565474, Length=188, Percent_Identity=32.4468085106383, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565698, Length=295, Percent_Identity=28.135593220339, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320608, Length=262, Percent_Identity=30.9160305343511, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24641839, Length=193, Percent_Identity=34.1968911917098, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583915, Length=194, Percent_Identity=34.020618556701, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24641837, Length=328, Percent_Identity=30.4878048780488, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 32675; Mature: 32675

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPIILDTDPGIDDAAAIAAALFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNADV
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCC
PLAQGASMPLVRPLRDAASVHGESGMEGYDFVEHQRQPLAKPAFQAIRDALMHAAEPITL
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VAIGPLTNIALLLTQYPECVFNIRRLVIMGGSAGRGNFTPNAEFNIAIDPEAAAKVFHSG
EEECCHHHHHHHHHHCHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHCC
LEIVMCGLDVTNRALLAADYLATLPTLNQTGKMLHALFSHYRSGSMSSGLRMHDLCAIAW
CEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LARPELFTLQPCFVAVETQGTWTAGTTVVDIEGRLGQPANAQVALDIDVEGFQRWAAEVI
HCCCCEEEECCEEEEEECCCCEEECCEEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHH
ALAP
CCCC
>Mature Secondary Structure
MRLPIILDTDPGIDDAAAIAAALFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNADV
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCC
PLAQGASMPLVRPLRDAASVHGESGMEGYDFVEHQRQPLAKPAFQAIRDALMHAAEPITL
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VAIGPLTNIALLLTQYPECVFNIRRLVIMGGSAGRGNFTPNAEFNIAIDPEAAAKVFHSG
EEECCHHHHHHHHHHCHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHCC
LEIVMCGLDVTNRALLAADYLATLPTLNQTGKMLHALFSHYRSGSMSSGLRMHDLCAIAW
CEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LARPELFTLQPCFVAVETQGTWTAGTTVVDIEGRLGQPANAQVALDIDVEGFQRWAAEVI
HCCCCEEEECCEEEEEECCCCEEECCEEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHH
ALAP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; xanthosine; inosine; adenosine; cytidine; guanosine [C]

Specific reaction: uridine + H2O = uracil + ribose xanthosine + H2O = xanthine + ribose inosine + H2O = hypoxanthine + ribose adenosine + H2O = adenine + ribose cytidine + H2O = cytosine + ribose guanosine + H2O = guanine + ribose [C]

General reaction: Nonspecific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA