Click here to switch to the map view.

The map label for this gene is zraS [H]

Identifier: 238892479

GI number: 238892479

Start: 251289

End: 252683

Strand: Direct

Name: zraS [H]

Synonym: KP1_0245

Alternate gene names: 238892479

Gene position: 251289-252683 (Clockwise)

Preceding gene: 238892476

Following gene: 238892480

Centisome position: 4.79

GC content: 61.0

Gene sequence:

>1395_bases
ATGAAGATCAAATCTCTCTCCCGGGACGCCGCCGCTGGCGCGCTGAGCTGGCTGCTGACCGGCACGGTTGTGCTGCTGGT
CATGCTGTTCTCCGCGATGATCGTCCGCGACTACGGTCGCGAGACGACCGCTGCCCGACAAACCATTGAAGAGAAAGGGA
GCGTGCTGATCCGCGCGCTGGAGTCCGGCACGCGCGTCGGGATGGGGATGCGTATGCATCATGCGCAGCTGCAGGCCCTG
CTGGAAGAGATGGCCTGGCAACCGGGCGTCCTGTGGTTCGCGGTGACCGATGAAAACGGTAAAATTATCGCCCATAGCGA
TCCGCAGCAGGTGGGTAAAACGCTGTATAGCCCGGCGCAGATGCGTGCGTTAGCGGTTGGCGAACAGGCCCGCTGGCGCC
GGCTTAGCGAACCGCAGCCGGCGCTAGAGATCTATCGCCATTTTCGCCCGCTTAATCCGGCCCGCGGCCACCATATGGGA
ATGATGAACCGCGGCAATAGCGCCCTGGCTCAGGCCATCGTCCCGCAGGTGATTTTTATCGCCTTTGATAGCCGCGAGCT
GGATGCCGCGCAGGCCCGCGGGCAGCGAAATATGGCCATCATGCTGGGTGCCGCCGCGCTGGTGATAGCGGCGACGATCC
TCGCTCAGTTCTGGTTCCGCCGCTATCGTCGCTCGCGGAAACAGCTGCTGGAGGCGATGGCCCGCAAGGAAAAGCTGGTG
GCGCTTGGCCATCTGGCGGCGGGGGTCGCGCACGAGATCCGCAACCCGCTCTCGTCGATTAAAGGGCTGGCGAAATATTT
TGCCGAGCGCACGTCGCCTGGCGGCGAGTCGCATCAGTTAGCCCAGGTGATGGCCAAAGAGGCTGATCGCCTGAACCGGG
TGGTCAGCGAGCTGCTGGAGCTGGTCCGTCCCGCCCATCTAAACTACCAAACAGTCGATATCAACGCGCTTATCCGCCAC
TCATTGCAGTTGGTAAGCCAGGATGCGCAGAGCCGCGGGATCGCGCTGCAATTTACTCCGCGACCTGAGTTAACCACGAT
CAGCGCCGACCCCGATCGCCTGAATCAGGTGCTGTTGAACCTGTACCTGAATGCAATGCAGGCGATTGGTCGTGACGGCG
TCATTCGCGTAACAGCAAGCGAAGCCGATCGTCAGCGGGTGAAAATCGTCGTCACGGATAGCGGTAAGGGGATGAGCGAT
GAAGAGTTACAGGCTATCTTCACGCCGTATTTCACCACCAAGGCGGACGGCACCGGGCTGGGGCTGGCCGTAGTGCAGAA
TATTATCGAACAACATGGCGGGACGATCCGCGCCGAAAGTCAGCCGGGAGCTGGAGCGATATTTACGCTTTGGTTACCGG
TGGATGCTCAACGGAGGGAAGATGAGCAGCGATAA

Upstream 100 bases:

>100_bases
GGCGATATGTTTGAGAGCAGGATTGGCCGCGCGGAAACATGGCATGATTTCTGCTGAAGGAGCCTGCAGATAGTGAAAAA
GAGATGACAAGGACTCGCCG

Downstream 100 bases:

>100_bases
AGTACAGATTCTGGTCGTTGATGATGACATCAGCCACTGCACGATCCTGCAGGCGCTGCTGCGCGGCTGGGGCTATCAGG
TGGGGGTGGCTCACAACGGC

Product: sensor protein ZraS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKIKSLSRDAAAGALSWLLTGTVVLLVMLFSAMIVRDYGRETTAARQTIEEKGSVLIRALESGTRVGMGMRMHHAQLQAL
LEEMAWQPGVLWFAVTDENGKIIAHSDPQQVGKTLYSPAQMRALAVGEQARWRRLSEPQPALEIYRHFRPLNPARGHHMG
MMNRGNSALAQAIVPQVIFIAFDSRELDAAQARGQRNMAIMLGAAALVIAATILAQFWFRRYRRSRKQLLEAMARKEKLV
ALGHLAAGVAHEIRNPLSSIKGLAKYFAERTSPGGESHQLAQVMAKEADRLNRVVSELLELVRPAHLNYQTVDINALIRH
SLQLVSQDAQSRGIALQFTPRPELTTISADPDRLNQVLLNLYLNAMQAIGRDGVIRVTASEADRQRVKIVVTDSGKGMSD
EELQAIFTPYFTTKADGTGLGLAVVQNIIEQHGGTIRAESQPGAGAIFTLWLPVDAQRREDEQR

Sequences:

>Translated_464_residues
MKIKSLSRDAAAGALSWLLTGTVVLLVMLFSAMIVRDYGRETTAARQTIEEKGSVLIRALESGTRVGMGMRMHHAQLQAL
LEEMAWQPGVLWFAVTDENGKIIAHSDPQQVGKTLYSPAQMRALAVGEQARWRRLSEPQPALEIYRHFRPLNPARGHHMG
MMNRGNSALAQAIVPQVIFIAFDSRELDAAQARGQRNMAIMLGAAALVIAATILAQFWFRRYRRSRKQLLEAMARKEKLV
ALGHLAAGVAHEIRNPLSSIKGLAKYFAERTSPGGESHQLAQVMAKEADRLNRVVSELLELVRPAHLNYQTVDINALIRH
SLQLVSQDAQSRGIALQFTPRPELTTISADPDRLNQVLLNLYLNAMQAIGRDGVIRVTASEADRQRVKIVVTDSGKGMSD
EELQAIFTPYFTTKADGTGLGLAVVQNIIEQHGGTIRAESQPGAGAIFTLWLPVDAQRREDEQR
>Mature_464_residues
MKIKSLSRDAAAGALSWLLTGTVVLLVMLFSAMIVRDYGRETTAARQTIEEKGSVLIRALESGTRVGMGMRMHHAQLQAL
LEEMAWQPGVLWFAVTDENGKIIAHSDPQQVGKTLYSPAQMRALAVGEQARWRRLSEPQPALEIYRHFRPLNPARGHHMG
MMNRGNSALAQAIVPQVIFIAFDSRELDAAQARGQRNMAIMLGAAALVIAATILAQFWFRRYRRSRKQLLEAMARKEKLV
ALGHLAAGVAHEIRNPLSSIKGLAKYFAERTSPGGESHQLAQVMAKEADRLNRVVSELLELVRPAHLNYQTVDINALIRH
SLQLVSQDAQSRGIALQFTPRPELTTISADPDRLNQVLLNLYLNAMQAIGRDGVIRVTASEADRQRVKIVVTDSGKGMSD
EELQAIFTPYFTTKADGTGLGLAVVQNIIEQHGGTIRAESQPGAGAIFTLWLPVDAQRREDEQR

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=465, Percent_Identity=63.8709677419355, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1788549, Length=282, Percent_Identity=40.0709219858156, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1790300, Length=242, Percent_Identity=32.2314049586777, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788713, Length=237, Percent_Identity=31.6455696202532, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790346, Length=240, Percent_Identity=30, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=30.3964757709251, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994928, Length=245, Percent_Identity=28.9795918367347, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=29.0178571428571, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081816, Length=217, Percent_Identity=28.110599078341, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786912, Length=218, Percent_Identity=28.8990825688073, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789808, Length=219, Percent_Identity=25.1141552511416, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790861, Length=237, Percent_Identity=27.8481012658228, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=25.1101321585903, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789149, Length=224, Percent_Identity=20.9821428571429, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786783, Length=229, Percent_Identity=25.3275109170306, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51295; Mature: 51295

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKSLSRDAAAGALSWLLTGTVVLLVMLFSAMIVRDYGRETTAARQTIEEKGSVLIRAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ESGTRVGMGMRMHHAQLQALLEEMAWQPGVLWFAVTDENGKIIAHSDPQQVGKTLYSPAQ
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCHHHHHHHHCCHHH
MRALAVGEQARWRRLSEPQPALEIYRHFRPLNPARGHHMGMMNRGNSALAQAIVPQVIFI
HHHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEE
AFDSRELDAAQARGQRNMAIMLGAAALVIAATILAQFWFRRYRRSRKQLLEAMARKEKLV
EECCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGHLAAGVAHEIRNPLSSIKGLAKYFAERTSPGGESHQLAQVMAKEADRLNRVVSELLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVRPAHLNYQTVDINALIRHSLQLVSQDAQSRGIALQFTPRPELTTISADPDRLNQVLLN
HHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCHHHHHHHHHH
LYLNAMQAIGRDGVIRVTASEADRQRVKIVVTDSGKGMSDEELQAIFTPYFTTKADGTGL
HHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEECCCCCCCH
GLAVVQNIIEQHGGTIRAESQPGAGAIFTLWLPVDAQRREDEQR
HHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCHHCCCCCC
>Mature Secondary Structure
MKIKSLSRDAAAGALSWLLTGTVVLLVMLFSAMIVRDYGRETTAARQTIEEKGSVLIRAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ESGTRVGMGMRMHHAQLQALLEEMAWQPGVLWFAVTDENGKIIAHSDPQQVGKTLYSPAQ
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCHHHHHHHHCCHHH
MRALAVGEQARWRRLSEPQPALEIYRHFRPLNPARGHHMGMMNRGNSALAQAIVPQVIFI
HHHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEE
AFDSRELDAAQARGQRNMAIMLGAAALVIAATILAQFWFRRYRRSRKQLLEAMARKEKLV
EECCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGHLAAGVAHEIRNPLSSIKGLAKYFAERTSPGGESHQLAQVMAKEADRLNRVVSELLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVRPAHLNYQTVDINALIRHSLQLVSQDAQSRGIALQFTPRPELTTISADPDRLNQVLLN
HHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCHHHHHHHHHH
LYLNAMQAIGRDGVIRVTASEADRQRVKIVVTDSGKGMSDEELQAIFTPYFTTKADGTGL
HHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEECCCCCCCH
GLAVVQNIIEQHGGTIRAESQPGAGAIFTLWLPVDAQRREDEQR
HHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]