Click here to switch to the map view.

The map label for this gene is nfi [H]

Identifier: 238892473

GI number: 238892473

Start: 248117

End: 248788

Strand: Direct

Name: nfi [H]

Synonym: KP1_0238

Alternate gene names: 238892473

Gene position: 248117-248788 (Clockwise)

Preceding gene: 238892472

Following gene: 238892474

Centisome position: 4.73

GC content: 61.16

Gene sequence:

>672_bases
ATGGATTTAGCCGCACTACGTACCCAACAGCAACAGTTAGCCGCGTCGGTAGAGCGTGCGGATCGACTGGATCGCGACCC
GCCGGCGTTGATCGGCGGCGCGGACGTCGGGTTTGAGCAGGAAGGGGAGATCACCCGGGCTGCGATGGTGCTGCTGACCT
GGCCTGAGCTTGAGCTGGTGGAGTATCAGGTGGCGCGGGTCGCCACCTCTATGCCCTATATTCCCGGCTTTCTCTCGTTT
CGCGAAACGCCTGCGCTGCTGGCGGCGTGGGAGCAGCTTTCGCAAAAACCGAACCTACTCTTCGTTGACGGACACGGCAT
TTCTCATCCCCGGAGATTGGGCGTCGCCAGCCATTTTGGCCTGATGATTGATGTGCCGACTATCGGAGTGGCGAAAAAAC
GCCTGTGCGGCAAAATCGGCGACCTGGGTGATGAGCCTGGCGCGCTGGCCCCGCTGATGGATAAAAATGAGCAACTGGCC
TGGGTCTGGCGCAGCAAAGTGCGCTGCAACCCGCTGTTTATTAGCACCGGCCACCGGGTAGGCATGGACAGCGCTTTAAT
GTGGGTTGAGCGCTGCATGCGAGGCTACCGTCTGCCCGAGCCGACGCGTTGGGCCGATGCGGTGGCGTCCCGTCGTCCGT
CGTTTGTGCGTTGGCAAGCAAATCACAGCTGA

Upstream 100 bases:

>100_bases
ACACGGTATTCATCAGGACGTTGACCCCGAGCATGCGGGCGTCTTTGTTGAGGCTGTGCACCGTCTGTCCGCACCCTATC
ACCAGTAGGAGATAAGCCCT

Downstream 100 bases:

>100_bases
TTCAGGTACACTGCGCCTAATTTCTGACTTCGAGAATTAATCATGTTACAAAACCCGATTCACCTGCGCCTGGAGAAGCT
GGAAAGCTGGCAGCATGTCA

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MDLAALRTQQQQLAASVERADRLDRDPPALIGGADVGFEQEGEITRAAMVLLTWPELELVEYQVARVATSMPYIPGFLSF
RETPALLAAWEQLSQKPNLLFVDGHGISHPRRLGVASHFGLMIDVPTIGVAKKRLCGKIGDLGDEPGALAPLMDKNEQLA
WVWRSKVRCNPLFISTGHRVGMDSALMWVERCMRGYRLPEPTRWADAVASRRPSFVRWQANHS

Sequences:

>Translated_223_residues
MDLAALRTQQQQLAASVERADRLDRDPPALIGGADVGFEQEGEITRAAMVLLTWPELELVEYQVARVATSMPYIPGFLSF
RETPALLAAWEQLSQKPNLLFVDGHGISHPRRLGVASHFGLMIDVPTIGVAKKRLCGKIGDLGDEPGALAPLMDKNEQLA
WVWRSKVRCNPLFISTGHRVGMDSALMWVERCMRGYRLPEPTRWADAVASRRPSFVRWQANHS
>Mature_223_residues
MDLAALRTQQQQLAASVERADRLDRDPPALIGGADVGFEQEGEITRAAMVLLTWPELELVEYQVARVATSMPYIPGFLSF
RETPALLAAWEQLSQKPNLLFVDGHGISHPRRLGVASHFGLMIDVPTIGVAKKRLCGKIGDLGDEPGALAPLMDKNEQLA
WVWRSKVRCNPLFISTGHRVGMDSALMWVERCMRGYRLPEPTRWADAVASRRPSFVRWQANHS

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=200, Percent_Identity=39.5, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI257467548, Length=195, Percent_Identity=31.7948717948718, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI257467550, Length=195, Percent_Identity=31.7948717948718, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI87082357, Length=222, Percent_Identity=81.0810810810811, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 24883; Mature: 24883

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAALRTQQQQLAASVERADRLDRDPPALIGGADVGFEQEGEITRAAMVLLTWPELELV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCCHHHH
EYQVARVATSMPYIPGFLSFRETPALLAAWEQLSQKPNLLFVDGHGISHPRRLGVASHFG
HHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCHHHHCC
LMIDVPTIGVAKKRLCGKIGDLGDEPGALAPLMDKNEQLAWVWRSKVRCNPLFISTGHRV
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCC
GMDSALMWVERCMRGYRLPEPTRWADAVASRRPSFVRWQANHS
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure
MDLAALRTQQQQLAASVERADRLDRDPPALIGGADVGFEQEGEITRAAMVLLTWPELELV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCCHHHH
EYQVARVATSMPYIPGFLSFRETPALLAAWEQLSQKPNLLFVDGHGISHPRRLGVASHFG
HHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCHHHHCC
LMIDVPTIGVAKKRLCGKIGDLGDEPGALAPLMDKNEQLAWVWRSKVRCNPLFISTGHRV
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCC
GMDSALMWVERCMRGYRLPEPTRWADAVASRRPSFVRWQANHS
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA