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The map label for this gene is metE [H]

Identifier: 238892427

GI number: 238892427

Start: 189925

End: 192189

Strand: Direct

Name: metE [H]

Synonym: KP1_0187

Alternate gene names: 238892427

Gene position: 189925-192189 (Clockwise)

Preceding gene: 238892424

Following gene: 238892429

Centisome position: 3.62

GC content: 60.66

Gene sequence:

>2265_bases
ATGACAATTATTAACCACACCCTCGGTTTTCCTCGCGTTGGCCTGCGCCGCGAGCTGAAAAAAGCGCAAGAGAGCTACTG
GGCGGGCAACGCCACTCGCGAGGAACTGCTGGCGGTAGGCCGTGAGCTGCGCGCCCGTCACTGGGAGCAGCAGAAACAGG
CGGGCGTTGATCTGCTGCCGGTGGGGGATTTCGCCTGGTACGACCATGTTCTGACTACCAGCCTGCTGCTTGGCAACGTG
CCGGCTCGTCATCAGAACAAAGACGGATCCATCGACATCGATACCCTGTTCCGCATTGGCCGCGGCCGCGCGCCGACTGG
CGAACCGGCCGCCGCTGCGGAAATGACCAAATGGTTTAACACCAACTATCACTATATGGTGCCGGAGTTCGTGAAGGGCC
AGCAGTTCAAACTGAGCTGGACCCAACTGCTGGATGAAGTAGACGAAGCGCTGGCGCTGGGCCACAAGATCAAGCCCGTG
CTGCTGGGGCCGGTAACCTATCTGTGGCTGGGTAAAGTGAAAGGTGAACCGTTTGACCGCCTGACCCTGCTGAATGCCAT
CCTGCCGGTTTACCAGCAGGTGCTGGCCGAACTGGCGAAGCGCGGCATCGATTGGGTGCAGATTGATGAACCGGCGCTGG
TGCTGGAGCTGCCGCCAGCGTGGCTGGAGGCCTTTCAACCGGCTTATGACGCCCTGCAGGGCCAGGTGAAGCTGCTGCTG
ACTACCTATTTTGAAGGCGTTAGCGACAATCTCGCGACCATTGCTGCGCTACCGGTCCAGGGGCTGCACGTCGATCTGGT
GCACGGCAAAGATGATGTTGCCGAACTGCACAACCGCCTGCCGGCCGACTGGCTGCTGTCCGCGGGCTTGATCAATGGGC
GTAACGTCTGGCGCGCCGATCTCACCGAAAAGTATGCGCAAATTAAAGACCTCGTCGGTAAGCGCGAGCTATGGGTCGCG
TCATCCTGCTCTCTGCTGCATAGCCCGATCGATCTGAGCGTGGAGACGCGCCTTGATGCGGAAGTGAAGAGCTGGTTTGC
CTTCGCGCTGCAGAAATGCGGTGAGCTGGCGCTGCTGCGCGATGCGCTGAACAGCGGCGATACGGCGGCGATTACCGAGT
GGAGCGCGCCAATCCAGGCCCGTCGCCATTCGACCCGCGTGCACAACGCGGAGGTGGAAAAACGGCTGGCGGCGATCACT
GCTCAGGACAGCCAGCGCGCCAGCCCCTATGAGGTTCGCGCGCAGGCACAGCGCCAGCGCTTTAACCTGCCGAAATGGCC
GACTACCACCATCGGGTCATTCCCGCAAACGACCGAGATCCGCGGCCTGCGCCTGGACTTCAAAAAGGGTAACCTTGACG
CCAGTCATTATCGGACGGGGATTGCGGAACATATTAAGCAGGCGATCGTCGAGCAGGAGCGGTTAGGTTTAGACGTACTG
GTGCACGGGGAGGCCGAGCGGAACGACATGGTGGAATACTTCGGCGAACATCTGGACGGTTTTATCTTCACGCAAAACGG
CTGGGTGCAGAGCTATGGTTCCCGCTGCGTGAAGCCGCCGGTGGTGATCGGCGACGTCAGCCGTCCGCAGGCGATCACCG
TCGACTGGGCGAAGTACGCGCAGTCGCTGACCGACAAGCCGGTGAAAGGGATGCTCACTGGACCGGTGACCATTCTGTGC
TGGTCCTTCCCGCGGGAGGATGTCAGCCGTGAAACCATCGCGAAACAGATTGCGCTGGCCCTGCGTGATGAAGTGGCGGA
TCTGGAGGCGGCGGGGATCGGCATCATCCAGATTGATGAGCCGGCGCTGCGCGAAGGGTTACCGCTGAAGCGCAGCGACT
GGGATGCCTATCTGCAGTGGGGTGTGGAAGCCTTCCGCCTTAATGCTGCGGTCGCCAAAGACGACACGCAGATCCATACC
CATATGTGCTATTGCGAGTTCAATGACATCATGGACTCCATTGCCGCGCTGGACGCTGACGTGATCACCATTGAAACCTC
GCGGTCCGATATGGAGCTGCTGGAGTCGTTCGAAGCGTTCGAGTACCCCAATGAGATCGGGCCGGGAGTATATGATATTC
ACTCGCCAAACGTGCCGAGCGTGGAGTGGATCGAAGCGCTGCTGAAGAAAGCCGCGCAGCGTATCCCGGTGGAGCGACTG
TGGGTTAACCCGGACTGCGGCCTGAAAACTCGCGGCTGGCCGGAAACCCGCGCCGCGCTGGCGAATATGGTGCAAGCGGC
GCGAAATCTGCGCCAGTCCGCCTAA

Upstream 100 bases:

>100_bases
CTTCAGTAAGCTCCTGGGGATGCGCATCGTTGCCGCCTTCCTGCAAGCCGAACTATTGAGACTTTTTCTTCAGATTCAAT
GAAATAAGAGAGGAACAAAA

Downstream 100 bases:

>100_bases
TCGTTACGCGAAAAATCAAAAAGGAGGCATTTGCCTCCTTTTTTCATGCTTAGGGCGATTACCCTTTTTTACCGCCATAC
TGAGCAAACCAGGCGAGCAT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 754; Mature: 753

Protein sequence:

>754_residues
MTIINHTLGFPRVGLRRELKKAQESYWAGNATREELLAVGRELRARHWEQQKQAGVDLLPVGDFAWYDHVLTTSLLLGNV
PARHQNKDGSIDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLSWTQLLDEVDEALALGHKIKPV
LLGPVTYLWLGKVKGEPFDRLTLLNAILPVYQQVLAELAKRGIDWVQIDEPALVLELPPAWLEAFQPAYDALQGQVKLLL
TTYFEGVSDNLATIAALPVQGLHVDLVHGKDDVAELHNRLPADWLLSAGLINGRNVWRADLTEKYAQIKDLVGKRELWVA
SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCGELALLRDALNSGDTAAITEWSAPIQARRHSTRVHNAEVEKRLAAIT
AQDSQRASPYEVRAQAQRQRFNLPKWPTTTIGSFPQTTEIRGLRLDFKKGNLDASHYRTGIAEHIKQAIVEQERLGLDVL
VHGEAERNDMVEYFGEHLDGFIFTQNGWVQSYGSRCVKPPVVIGDVSRPQAITVDWAKYAQSLTDKPVKGMLTGPVTILC
WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLKRSDWDAYLQWGVEAFRLNAAVAKDDTQIHT
HMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEAFEYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPVERL
WVNPDCGLKTRGWPETRAALANMVQAARNLRQSA

Sequences:

>Translated_754_residues
MTIINHTLGFPRVGLRRELKKAQESYWAGNATREELLAVGRELRARHWEQQKQAGVDLLPVGDFAWYDHVLTTSLLLGNV
PARHQNKDGSIDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLSWTQLLDEVDEALALGHKIKPV
LLGPVTYLWLGKVKGEPFDRLTLLNAILPVYQQVLAELAKRGIDWVQIDEPALVLELPPAWLEAFQPAYDALQGQVKLLL
TTYFEGVSDNLATIAALPVQGLHVDLVHGKDDVAELHNRLPADWLLSAGLINGRNVWRADLTEKYAQIKDLVGKRELWVA
SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCGELALLRDALNSGDTAAITEWSAPIQARRHSTRVHNAEVEKRLAAIT
AQDSQRASPYEVRAQAQRQRFNLPKWPTTTIGSFPQTTEIRGLRLDFKKGNLDASHYRTGIAEHIKQAIVEQERLGLDVL
VHGEAERNDMVEYFGEHLDGFIFTQNGWVQSYGSRCVKPPVVIGDVSRPQAITVDWAKYAQSLTDKPVKGMLTGPVTILC
WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLKRSDWDAYLQWGVEAFRLNAAVAKDDTQIHT
HMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEAFEYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPVERL
WVNPDCGLKTRGWPETRAALANMVQAARNLRQSA
>Mature_753_residues
TIINHTLGFPRVGLRRELKKAQESYWAGNATREELLAVGRELRARHWEQQKQAGVDLLPVGDFAWYDHVLTTSLLLGNVP
ARHQNKDGSIDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLSWTQLLDEVDEALALGHKIKPVL
LGPVTYLWLGKVKGEPFDRLTLLNAILPVYQQVLAELAKRGIDWVQIDEPALVLELPPAWLEAFQPAYDALQGQVKLLLT
TYFEGVSDNLATIAALPVQGLHVDLVHGKDDVAELHNRLPADWLLSAGLINGRNVWRADLTEKYAQIKDLVGKRELWVAS
SCSLLHSPIDLSVETRLDAEVKSWFAFALQKCGELALLRDALNSGDTAAITEWSAPIQARRHSTRVHNAEVEKRLAAITA
QDSQRASPYEVRAQAQRQRFNLPKWPTTTIGSFPQTTEIRGLRLDFKKGNLDASHYRTGIAEHIKQAIVEQERLGLDVLV
HGEAERNDMVEYFGEHLDGFIFTQNGWVQSYGSRCVKPPVVIGDVSRPQAITVDWAKYAQSLTDKPVKGMLTGPVTILCW
SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLKRSDWDAYLQWGVEAFRLNAAVAKDDTQIHTH
MCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEAFEYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPVERLW
VNPDCGLKTRGWPETRAALANMVQAARNLRQSA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=752, Percent_Identity=93.0851063829787, Blast_Score=1456, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=762, Percent_Identity=48.8188976377953, Blast_Score=713, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 84519; Mature: 84388

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIINHTLGFPRVGLRRELKKAQESYWAGNATREELLAVGRELRARHWEQQKQAGVDLLP
CEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPARHQNKDGSIDIDTLFRIGRGRAPTGEPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFVKGQQFKLSWTQLLDEVDEALALGHKIKPVLLGPVTYLWLGKVKGEPFDR
CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCCCHHH
LTLLNAILPVYQQVLAELAKRGIDWVQIDEPALVLELPPAWLEAFQPAYDALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
TTYFEGVSDNLATIAALPVQGLHVDLVHGKDDVAELHNRLPADWLLSAGLINGRNVWRAD
HHHHHCCCCCHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHCCCCCCCCCCHHH
LTEKYAQIKDLVGKRELWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCGELALLR
HHHHHHHHHHHHCCHHEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHCHHHHHH
DALNSGDTAAITEWSAPIQARRHSTRVHNAEVEKRLAAITAQDSQRASPYEVRAQAQRQR
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FNLPKWPTTTIGSFPQTTEIRGLRLDFKKGNLDASHYRTGIAEHIKQAIVEQERLGLDVL
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
VHGEAERNDMVEYFGEHLDGFIFTQNGWVQSYGSRCVKPPVVIGDVSRPQAITVDWAKYA
EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHH
QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE
HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
PALREGLPLKRSDWDAYLQWGVEAFRLNAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD
HHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHCCCC
VITIETSRSDMELLESFEAFEYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPVERL
EEEEECCCHHHHHHHHHHHHCCCHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE
WVNPDCGLKTRGWPETRAALANMVQAARNLRQSA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIINHTLGFPRVGLRRELKKAQESYWAGNATREELLAVGRELRARHWEQQKQAGVDLLP
EEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPARHQNKDGSIDIDTLFRIGRGRAPTGEPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFVKGQQFKLSWTQLLDEVDEALALGHKIKPVLLGPVTYLWLGKVKGEPFDR
CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCCCHHH
LTLLNAILPVYQQVLAELAKRGIDWVQIDEPALVLELPPAWLEAFQPAYDALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
TTYFEGVSDNLATIAALPVQGLHVDLVHGKDDVAELHNRLPADWLLSAGLINGRNVWRAD
HHHHHCCCCCHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHCCCCCCCCCCHHH
LTEKYAQIKDLVGKRELWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCGELALLR
HHHHHHHHHHHHCCHHEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHCHHHHHH
DALNSGDTAAITEWSAPIQARRHSTRVHNAEVEKRLAAITAQDSQRASPYEVRAQAQRQR
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FNLPKWPTTTIGSFPQTTEIRGLRLDFKKGNLDASHYRTGIAEHIKQAIVEQERLGLDVL
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
VHGEAERNDMVEYFGEHLDGFIFTQNGWVQSYGSRCVKPPVVIGDVSRPQAITVDWAKYA
EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHH
QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE
HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
PALREGLPLKRSDWDAYLQWGVEAFRLNAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD
HHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHCCCC
VITIETSRSDMELLESFEAFEYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPVERL
EEEEECCCHHHHHHHHHHHHCCCHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE
WVNPDCGLKTRGWPETRAALANMVQAARNLRQSA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA