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The map label for this gene is pfkA [H]

Identifier: 238892300

GI number: 238892300

Start: 50734

End: 51786

Strand: Direct

Name: pfkA [H]

Synonym: KP1_0050

Alternate gene names: 238892300

Gene position: 50734-51786 (Clockwise)

Preceding gene: 238892299

Following gene: 238892301

Centisome position: 0.97

GC content: 44.63

Gene sequence:

>1053_bases
GTGATCTTCATTGCAAATAGAAGGCTTGTACTCAGGGAAAAATGTTGTCAGGCAAATGAGCGAGGATGCGTCATGAAAAT
TGGTATCGTTATTAGCGGCGGGGATGTTTCTGGAATGAATAATTTTATATTCCAGATAGCCAGGCAGGCTAATGCTGATA
TCACGCTGTTTAATGGCGGTATTCCAGGGCTACTGGAGAAAAGTCATCAGGATATGGCGTGGCGCGATCTGGTTGATTAT
TCAATTACCGCGGTGCCGATCATTACCTCGGGGCGAACCAGTCGCAAACTGCAGCGCAGTGAATATGAAAGCATTGCTAA
AAAACTTAAATCTCTGCGTATTGATGTGCTCATTATGGCCGGGGGGGATGGCAGCCTGCAGTTTCTGAATACCCTTAGTG
AATTTGAGATTAATTGCTTTGGTGTGGGAATGACTATCGATAATGATGTGTATGGCAGTGATTATACGATTGGTTTTTCC
ACGGCCTGTGAGCAAATTATTAAAGAAGTCTCTCGTTTAAGAAATACCGGAAGAGCGCTGCCCGGTCGGGTTTTTATGGT
TGAGATATTAGGAGGCTATTGTGGAGAACTAACGTTGCAGTCCGCCATTAAATGTAATGCGGATATTGCCCTGATCCCGG
AGGCACAGATGCCGTTGGCCGTGCTCGCGGAACGGATTACCCGGAAGCTGAGTACGCAGAATAGCGTCGTTATCCTCTGC
TCAGAAGGCTATACCAAAGAGTACTCTCCCGGCTTTCAGGGGGCGATTGATACCATGATTAAACAGCTGGAACCACAAAT
AGGTGTCCGTATCCGTAAAACGATCGTCGGCTATGGTTTACGCAGCGGTGATCCAACCTGCGAAGAGATTTATCAGGGCA
CTATTATGGCCAGTGAAGTGGTTCGCTGTATTCAGTCAGGTATGAAAAATAAAGCCATAATAATTAATTCCAGTAACAAA
CCTATTCCGATTGATTTAGTCAGTATGAAAAAACGTCTGGTCGATACTGAAGGACATCATTATAAACTTGCCAAGCAACT
CAATATTATTTGA

Upstream 100 bases:

>100_bases
AGCGATGGAGATCACTCTATTTAAATGGGATATGTATCACAAAAATAGGTGTTTCGCCGTACCTGTCAACTATTCACTGC
GGTAATTTAATACGCATTAT

Downstream 100 bases:

>100_bases
GGAGACCCTCCATGCTGAAAATTTTATGCGTATGTGGCTGCGGCCTCGGTTCAAGCTTTGCCATTGAAATGACGGCGAAG
GCAGTTTTAAAGAAGCTGGA

Product: putative 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MIFIANRRLVLREKCCQANERGCVMKIGIVISGGDVSGMNNFIFQIARQANADITLFNGGIPGLLEKSHQDMAWRDLVDY
SITAVPIITSGRTSRKLQRSEYESIAKKLKSLRIDVLIMAGGDGSLQFLNTLSEFEINCFGVGMTIDNDVYGSDYTIGFS
TACEQIIKEVSRLRNTGRALPGRVFMVEILGGYCGELTLQSAIKCNADIALIPEAQMPLAVLAERITRKLSTQNSVVILC
SEGYTKEYSPGFQGAIDTMIKQLEPQIGVRIRKTIVGYGLRSGDPTCEEIYQGTIMASEVVRCIQSGMKNKAIIINSSNK
PIPIDLVSMKKRLVDTEGHHYKLAKQLNII

Sequences:

>Translated_350_residues
MIFIANRRLVLREKCCQANERGCVMKIGIVISGGDVSGMNNFIFQIARQANADITLFNGGIPGLLEKSHQDMAWRDLVDY
SITAVPIITSGRTSRKLQRSEYESIAKKLKSLRIDVLIMAGGDGSLQFLNTLSEFEINCFGVGMTIDNDVYGSDYTIGFS
TACEQIIKEVSRLRNTGRALPGRVFMVEILGGYCGELTLQSAIKCNADIALIPEAQMPLAVLAERITRKLSTQNSVVILC
SEGYTKEYSPGFQGAIDTMIKQLEPQIGVRIRKTIVGYGLRSGDPTCEEIYQGTIMASEVVRCIQSGMKNKAIIINSSNK
PIPIDLVSMKKRLVDTEGHHYKLAKQLNII
>Mature_350_residues
MIFIANRRLVLREKCCQANERGCVMKIGIVISGGDVSGMNNFIFQIARQANADITLFNGGIPGLLEKSHQDMAWRDLVDY
SITAVPIITSGRTSRKLQRSEYESIAKKLKSLRIDVLIMAGGDGSLQFLNTLSEFEINCFGVGMTIDNDVYGSDYTIGFS
TACEQIIKEVSRLRNTGRALPGRVFMVEILGGYCGELTLQSAIKCNADIALIPEAQMPLAVLAERITRKLSTQNSVVILC
SEGYTKEYSPGFQGAIDTMIKQLEPQIGVRIRKTIVGYGLRSGDPTCEEIYQGTIMASEVVRCIQSGMKNKAIIINSSNK
PIPIDLVSMKKRLVDTEGHHYKLAKQLNII

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=336, Percent_Identity=24.4047619047619, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI266453619, Length=355, Percent_Identity=24.2253521126761, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI266453768, Length=352, Percent_Identity=24.4318181818182, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI266453748, Length=352, Percent_Identity=24.4318181818182, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4505749, Length=352, Percent_Identity=24.4318181818182, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI11321601, Length=322, Percent_Identity=27.9503105590062, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1790350, Length=333, Percent_Identity=28.2282282282282, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25147584, Length=308, Percent_Identity=27.9220779220779, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17558788, Length=338, Percent_Identity=26.0355029585799, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI72003601, Length=295, Percent_Identity=23.0508474576271, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321679, Length=313, Percent_Identity=25.5591054313099, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323861, Length=345, Percent_Identity=23.768115942029, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI17647809, Length=342, Percent_Identity=23.9766081871345, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652337, Length=342, Percent_Identity=23.9766081871345, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28573326, Length=330, Percent_Identity=24.5454545454545, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 38586; Mature: 38586

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFIANRRLVLREKCCQANERGCVMKIGIVISGGDVSGMNNFIFQIARQANADITLFNGG
CEEEECCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECC
IPGLLEKSHQDMAWRDLVDYSITAVPIITSGRTSRKLQRSEYESIAKKLKSLRIDVLIMA
CCHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCEEEEEEEE
GGDGSLQFLNTLSEFEINCFGVGMTIDNDVYGSDYTIGFSTACEQIIKEVSRLRNTGRAL
CCCCCHHHHHHHHHCEEEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCC
PGRVFMVEILGGYCGELTLQSAIKCNADIALIPEAQMPLAVLAERITRKLSTQNSVVILC
CCCEEHEEHHCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
SEGYTKEYSPGFQGAIDTMIKQLEPQIGVRIRKTIVGYGLRSGDPTCEEIYQGTIMASEV
CCCCCCCCCCCCHHHHHHHHHHHCHHHCCEEEEHHHHCCCCCCCCCHHHHHCCHHHHHHH
VRCIQSGMKNKAIIINSSNKPIPIDLVSMKKRLVDTEGHHYKLAKQLNII
HHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHCCCCCCEEEHHHHHCCC
>Mature Secondary Structure
MIFIANRRLVLREKCCQANERGCVMKIGIVISGGDVSGMNNFIFQIARQANADITLFNGG
CEEEECCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECC
IPGLLEKSHQDMAWRDLVDYSITAVPIITSGRTSRKLQRSEYESIAKKLKSLRIDVLIMA
CCHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCEEEEEEEE
GGDGSLQFLNTLSEFEINCFGVGMTIDNDVYGSDYTIGFSTACEQIIKEVSRLRNTGRAL
CCCCCHHHHHHHHHCEEEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCC
PGRVFMVEILGGYCGELTLQSAIKCNADIALIPEAQMPLAVLAERITRKLSTQNSVVILC
CCCEEHEEHHCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
SEGYTKEYSPGFQGAIDTMIKQLEPQIGVRIRKTIVGYGLRSGDPTCEEIYQGTIMASEV
CCCCCCCCCCCCHHHHHHHHHHHCHHHCCEEEEHHHHCCCCCCCCCHHHHHCCHHHHHHH
VRCIQSGMKNKAIIINSSNKPIPIDLVSMKKRLVDTEGHHYKLAKQLNII
HHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHCCCCCCEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA