Click here to switch to the map view.

The map label for this gene is fdoH [H]

Identifier: 238892294

GI number: 238892294

Start: 45104

End: 46006

Strand: Reverse

Name: fdoH [H]

Synonym: KP1_0041

Alternate gene names: 238892294

Gene position: 46006-45104 (Counterclockwise)

Preceding gene: 238892295

Following gene: 238892293

Centisome position: 0.88

GC content: 59.8

Gene sequence:

>903_bases
ATGGCTTACCAATCGCAAGATATTATTCGTCGTTCCGCGACTAACGGTTTCACCCCCGCGCCTCAGGCGCGGGACCACCA
GCAGGAGGTGGCGAAGCTTATCGACGTCACCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCCTGCTCCGAATGGAACG
ATATTCGTGATGAAGTCGGTCACAATGTCGGGGTGTATGACAACCCGGCGGACCTGACCGCCAAGTCATGGACGGTCATG
CGCTTCTCGGAGGTGGAACAAAACGACAAGCTGGAATGGCTCATCCGCAAGGATGGCTGCATGCACTGCGCGGACCCGGG
CTGCCTGAAGGCCTGCCCGTCAGAAGGCGCGATCATTCAGTACGCCAACGGCATTGTCGACTTCCAGTCCGAGCAGTGCA
TCGGCTGCGGCTACTGTATCGCCGGCTGTCCGTTCGATGTACCGCGTCTGAACCCGGAAGACAACCGCGTCTACAAATGT
ACCCTGTGCGTAGACCGCGTGACCGTCGGCCAGGAGCCTGCCTGCGTGAAAACCTGTCCGACCGGCGCAATCCACTTTGG
CTCCAAAGAGGATATGAAAACGCTGGCCGGCGAACGCGTGGCTGAGCTGAAAACCCGCGGTTATGACAATGCGGGCCTGT
ACGATCCGGCCGGCGTCGGTGGCACCCACGTCATGTACGTGCTGCACCATGCCGACAAGCCGAATCTGTACCACGGCCTG
CCGGAAAACCCGGAGATCAGCCAGACCGTCAAGTTCTGGAAAGGCATCTGGAAACCGCTGGCGGCCGTCGGCTTCGCCGC
GACCTTCGCAGCCAGTATCTTCCACTACGTCGGCGTCGGTCCGAACCGCGCGGAAGAGGAAGAGGACAATCTGCATGAAG
AGAAAGACGAGGTGCGCAAATGA

Upstream 100 bases:

>100_bases
TGCCAATACCCTGACGCCGTTCGTCGGTGATGCGAATACGCAGACGCCGGAGTTCAAAGCTTTCCTTGTGAACGTGGAAA
AGGTGTAACGGAGACGACTT

Downstream 100 bases:

>100_bases
AAAGACGTGACACCATCGTGCGCTACACGGCGCCGGAACGCATCAACCACTGGGTCACCGCCTTCTGCTTCGTCCTGGCG
GCGGTGAGCGGGTTAGGCTT

Product: formate dehydrogenase-O beta subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MAYQSQDIIRRSATNGFTPAPQARDHQQEVAKLIDVTTCIGCKACQVACSEWNDIRDEVGHNVGVYDNPADLTAKSWTVM
RFSEVEQNDKLEWLIRKDGCMHCADPGCLKACPSEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDVPRLNPEDNRVYKC
TLCVDRVTVGQEPACVKTCPTGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
PENPEISQTVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRAEEEEDNLHEEKDEVRK

Sequences:

>Translated_300_residues
MAYQSQDIIRRSATNGFTPAPQARDHQQEVAKLIDVTTCIGCKACQVACSEWNDIRDEVGHNVGVYDNPADLTAKSWTVM
RFSEVEQNDKLEWLIRKDGCMHCADPGCLKACPSEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDVPRLNPEDNRVYKC
TLCVDRVTVGQEPACVKTCPTGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
PENPEISQTVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRAEEEEDNLHEEKDEVRK
>Mature_299_residues
AYQSQDIIRRSATNGFTPAPQARDHQQEVAKLIDVTTCIGCKACQVACSEWNDIRDEVGHNVGVYDNPADLTAKSWTVMR
FSEVEQNDKLEWLIRKDGCMHCADPGCLKACPSEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDVPRLNPEDNRVYKCT
LCVDRVTVGQEPACVKTCPTGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGLP
ENPEISQTVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRAEEEEDNLHEEKDEVRK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=300, Percent_Identity=93.6666666666667, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787749, Length=294, Percent_Identity=77.5510204081633, Blast_Score=496, Evalue=1e-142,
Organism=Escherichia coli, GI1789370, Length=184, Percent_Identity=32.0652173913043, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787872, Length=175, Percent_Identity=33.1428571428571, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787122, Length=169, Percent_Identity=32.5443786982249, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI2367345, Length=178, Percent_Identity=30.3370786516854, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI226510944, Length=162, Percent_Identity=30.8641975308642, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082179, Length=172, Percent_Identity=27.3255813953488, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787963, Length=154, Percent_Identity=24.6753246753247, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787740, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787478, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33088; Mature: 32956

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQSQDIIRRSATNGFTPAPQARDHQQEVAKLIDVTTCIGCKACQVACSEWNDIRDEVG
CCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
HNVGVYDNPADLTAKSWTVMRFSEVEQNDKLEWLIRKDGCMHCADPGCLKACPSEGAIIQ
CCCCCCCCCCCCCCCCEEEEEHHHCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEE
YANGIVDFQSEQCIGCGYCIAGCPFDVPRLNPEDNRVYKCTLCVDRVTVGQEPACVKTCP
ECCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEEHHHCCCCCCEEEECC
TGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
CCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
PENPEISQTVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRAEEEEDNLHEEKDEVRK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AYQSQDIIRRSATNGFTPAPQARDHQQEVAKLIDVTTCIGCKACQVACSEWNDIRDEVG
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
HNVGVYDNPADLTAKSWTVMRFSEVEQNDKLEWLIRKDGCMHCADPGCLKACPSEGAIIQ
CCCCCCCCCCCCCCCCEEEEEHHHCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEE
YANGIVDFQSEQCIGCGYCIAGCPFDVPRLNPEDNRVYKCTLCVDRVTVGQEPACVKTCP
ECCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEEHHHCCCCCCEEEECC
TGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
CCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
PENPEISQTVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRAEEEEDNLHEEKDEVRK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]