| Definition | Burkholderia glumae BGR1 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_012724 |
| Length | 3,906,507 |
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The map label for this gene is gshA [H]
Identifier: 238028953
GI number: 238028953
Start: 3885547
End: 3887160
Strand: Reverse
Name: gshA [H]
Synonym: bglu_1g34180
Alternate gene names: 238028953
Gene position: 3887160-3885547 (Counterclockwise)
Preceding gene: 238028954
Following gene: 238028952
Centisome position: 99.5
GC content: 66.29
Gene sequence:
>1614_bases ATGTCGAACAAGATGACTTCCAGCCACACCGACCTGCTGCAGCAACGTCTCGCCGTCCTGGCCAACGGGCCGACGCGTGA ACATCTGGTTGACGGCCTGCGCGGCATCGAGAAGGAAAGCCTGCGCGTGTCGCGCGACGGGATGATCGCGATGACGCCGC ATCCGCGCGCGCTCGGGTCCGCGCTCACGCATCCGGCGCTGACCACCGATTATTCGGAGTCGCTGATCGAGCTGATCACA CCCGCCGAGCGCGATGCGGCGATCACGCTCGACCGCCTCGACGATCTGCATCGTTTCGTTTATGCCTCGATCGGCGACGA GATGCTCTGGAACGTCTCGATGCCCGGCAAGCTGCCGGCCGACGACGAGATCCCGATCGCCGATTACGGCACCTCGAACA TCGGGCGGCTGAAGACGGTGTATCGCCGCGGCCTCGCCTATCGCTACGGCCGCACGATGCAGTGCATCGCGGGGGTCCAC TACAACTATTCGCTGCACGAGGACGTCTGGCGGCGCCTGCACGAACATGCGCAATCGGCCGCGTCGCTGGTCGATTACCA GTCCGAACGGTATCTCGCGCTGATCCGCAATTTCCGCCGCACCAGTTGGCTGCTGATGTATCTGTTCGGCGCGTCGCCGG CGCTCGACAAAGCATTCCTGCGCGGGCGCGACAGCACGCTCGAAGCGTTCGACGCCGATACGTTCTACCGGCCCTATGCG ACCAGCCTGCGGATGAGCGACCTCGGCTACTCGAACACCACTGCCCAGGCGGCGCTGGTAGCCGACTACAACTCGCTCGA TGCCTATCTCGACGCGTTGTCGAAGGCCGTCAGCGAGCCGTATCCGGCCTACGAGGCGATCGGCACGCATCGCGACGGCG AGTGGATCCAGCTCAACACCAACGTGCTGCAGATCGAGAACGAGTTCTACTCGACGATCCGGCCCAAGCGCGTGACGTAC TCGGGCGAGCGGCCGCTGCATGCACTGGCCTCGCGCGGCGTGCAGTACATCGAGGTGCGCTGCCTCGACATCGATCCGTT CGAGCCGACCGGGATCGCGCTCGAGACCGCGCGCTTCATGGATGCCTACCTGCTCGTCTGCGCGCTCGACGACAGCGCGA AGCTCGAATGCGAGGCCTACCGCGAGGCGAACGCGAATTTCGGCCGCGTGACCATGGAAGGGCGCAAGCCCGGACTCGAA CTGACGCGCGATGGCGTGCCGGTGCCGATGCGGGCCTGGGCCGAGGAAGTGCTCGCGAAGATCGAAACGGCGGCGCGCGT GCTCGACGCCATCCGCGGCGACGACGTGCATGCGCGCGCGGTGGCGGCGCAGCGTGCGAAGCTGGAGGATGCCGACAGGA CGCCCTCGGCGCGTGTGTTGCACGAGATGCGCGAGCGCGGCGAATCGTTCCTCGATTTCGCCCGCCGGCATAGCGCGGCG CATGCCGAGTATTTCCGGGCGCGGCCGCTCGATGCCGCGGCACTGGCCGAGGCGCGCGGGCAAGCCGAACGCTCGCTCGC CGAGCAGGCCGAGCTCGAAAGCAAGGATGCCGGATCGTTCGATGCGTTTGTCGCCGCCTATCGCGCTTACACGCTGAACC GTTTCAGCGTCTGA
Upstream 100 bases:
>100_bases TTCAAGCGAGCCTGACGGCGAGATGTTAAAGTGCCCGCTACCGTCAATCCCGGCGGGCGGCGCGTATGGGAGCGAGCCGC CACGCCGCACCTGTGCATCC
Downstream 100 bases:
>100_bases CGAGCACAGACGGGATCGTTGCGGGCGCAACGGTCCCGTCTGTCAATCATGGGGCGAGCCGTTGGCCGAGGTTTGCCATC CCGTTTCCGGGCACATCGAG
Product: glutamate--cysteine ligase
Products: NA
Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]
Number of amino acids: Translated: 537; Mature: 536
Protein sequence:
>537_residues MSNKMTSSHTDLLQQRLAVLANGPTREHLVDGLRGIEKESLRVSRDGMIAMTPHPRALGSALTHPALTTDYSESLIELIT PAERDAAITLDRLDDLHRFVYASIGDEMLWNVSMPGKLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGVH YNYSLHEDVWRRLHEHAQSAASLVDYQSERYLALIRNFRRTSWLLMYLFGASPALDKAFLRGRDSTLEAFDADTFYRPYA TSLRMSDLGYSNTTAQAALVADYNSLDAYLDALSKAVSEPYPAYEAIGTHRDGEWIQLNTNVLQIENEFYSTIRPKRVTY SGERPLHALASRGVQYIEVRCLDIDPFEPTGIALETARFMDAYLLVCALDDSAKLECEAYREANANFGRVTMEGRKPGLE LTRDGVPVPMRAWAEEVLAKIETAARVLDAIRGDDVHARAVAAQRAKLEDADRTPSARVLHEMRERGESFLDFARRHSAA HAEYFRARPLDAAALAEARGQAERSLAEQAELESKDAGSFDAFVAAYRAYTLNRFSV
Sequences:
>Translated_537_residues MSNKMTSSHTDLLQQRLAVLANGPTREHLVDGLRGIEKESLRVSRDGMIAMTPHPRALGSALTHPALTTDYSESLIELIT PAERDAAITLDRLDDLHRFVYASIGDEMLWNVSMPGKLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGVH YNYSLHEDVWRRLHEHAQSAASLVDYQSERYLALIRNFRRTSWLLMYLFGASPALDKAFLRGRDSTLEAFDADTFYRPYA TSLRMSDLGYSNTTAQAALVADYNSLDAYLDALSKAVSEPYPAYEAIGTHRDGEWIQLNTNVLQIENEFYSTIRPKRVTY SGERPLHALASRGVQYIEVRCLDIDPFEPTGIALETARFMDAYLLVCALDDSAKLECEAYREANANFGRVTMEGRKPGLE LTRDGVPVPMRAWAEEVLAKIETAARVLDAIRGDDVHARAVAAQRAKLEDADRTPSARVLHEMRERGESFLDFARRHSAA HAEYFRARPLDAAALAEARGQAERSLAEQAELESKDAGSFDAFVAAYRAYTLNRFSV >Mature_536_residues SNKMTSSHTDLLQQRLAVLANGPTREHLVDGLRGIEKESLRVSRDGMIAMTPHPRALGSALTHPALTTDYSESLIELITP AERDAAITLDRLDDLHRFVYASIGDEMLWNVSMPGKLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGVHY NYSLHEDVWRRLHEHAQSAASLVDYQSERYLALIRNFRRTSWLLMYLFGASPALDKAFLRGRDSTLEAFDADTFYRPYAT SLRMSDLGYSNTTAQAALVADYNSLDAYLDALSKAVSEPYPAYEAIGTHRDGEWIQLNTNVLQIENEFYSTIRPKRVTYS GERPLHALASRGVQYIEVRCLDIDPFEPTGIALETARFMDAYLLVCALDDSAKLECEAYREANANFGRVTMEGRKPGLEL TRDGVPVPMRAWAEEVLAKIETAARVLDAIRGDDVHARAVAAQRAKLEDADRTPSARVLHEMRERGESFLDFARRHSAAH AEYFRARPLDAAALAEARGQAERSLAEQAELESKDAGSFDAFVAAYRAYTLNRFSV
Specific function: Glutathione biosynthesis; first step. [C]
COG id: COG2918
COG function: function code H; Gamma-glutamylcysteine synthetase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]
Homologues:
Organism=Escherichia coli, GI1789044, Length=518, Percent_Identity=40.9266409266409, Blast_Score=375, Evalue=1e-105,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR007370 - InterPro: IPR006334 [H]
Pfam domain/function: PF04262 Glu_cys_ligase [H]
EC number: =6.3.2.2 [H]
Molecular weight: Translated: 60206; Mature: 60075
Theoretical pI: Translated: 5.90; Mature: 5.90
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSNKMTSSHTDLLQQRLAVLANGPTREHLVDGLRGIEKESLRVSRDGMIAMTPHPRALGS CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHEECCCCEEEECCCCHHHHH ALTHPALTTDYSESLIELITPAERDAAITLDRLDDLHRFVYASIGDEMLWNVSMPGKLPA HHCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCHHEEECCCCCCCCC DDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGVHYNYSLHEDVWRRLHEHAQSA CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHH ASLVDYQSERYLALIRNFRRTSWLLMYLFGASPALDKAFLRGRDSTLEAFDADTFYRPYA HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHCCCCCCCCHH TSLRMSDLGYSNTTAQAALVADYNSLDAYLDALSKAVSEPYPAYEAIGTHRDGEWIQLNT HHEEHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECC NVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALETARFM CEEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHH DAYLLVCALDDSAKLECEAYREANANFGRVTMEGRKPGLELTRDGVPVPMRAWAEEVLAK HHHEEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH IETAARVLDAIRGDDVHARAVAAQRAKLEDADRTPSARVLHEMRERGESFLDFARRHSAA HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH HAEYFRARPLDAAALAEARGQAERSLAEQAELESKDAGSFDAFVAAYRAYTLNRFSV HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC >Mature Secondary Structure SNKMTSSHTDLLQQRLAVLANGPTREHLVDGLRGIEKESLRVSRDGMIAMTPHPRALGS CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHEECCCCEEEECCCCHHHHH ALTHPALTTDYSESLIELITPAERDAAITLDRLDDLHRFVYASIGDEMLWNVSMPGKLPA HHCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCHHEEECCCCCCCCC DDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGVHYNYSLHEDVWRRLHEHAQSA CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHH ASLVDYQSERYLALIRNFRRTSWLLMYLFGASPALDKAFLRGRDSTLEAFDADTFYRPYA HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHCCCCCCCCHH TSLRMSDLGYSNTTAQAALVADYNSLDAYLDALSKAVSEPYPAYEAIGTHRDGEWIQLNT HHEEHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECC NVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALETARFM CEEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHH DAYLLVCALDDSAKLECEAYREANANFGRVTMEGRKPGLELTRDGVPVPMRAWAEEVLAK HHHEEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH IETAARVLDAIRGDDVHARAVAAQRAKLEDADRTPSARVLHEMRERGESFLDFARRHSAA HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH HAEYFRARPLDAAALAEARGQAERSLAEQAELESKDAGSFDAFVAAYRAYTLNRFSV HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA