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The map label for this gene is rlmI [H]

Identifier: 238028908

GI number: 238028908

Start: 3834414

End: 3835667

Strand: Direct

Name: rlmI [H]

Synonym: bglu_1g33730

Alternate gene names: 238028908

Gene position: 3834414-3835667 (Clockwise)

Preceding gene: 238028907

Following gene: 339905982

Centisome position: 98.15

GC content: 71.45

Gene sequence:

>1254_bases
ATGCAAACCGTTACGCTCAAACCGTCGAAAGACAAATCCCTGCTGCGCCGTCACCCGTGGATCTACGCAAACGCGATCGA
GCACGTGGACGGCAAGCCGGCCGCCGGCGCCACCGTGGTGGTGCGCTCGCACGACGGCCGCTTCCTCGCGCGCGGCGCCT
ACAGCCCGCATTCCCAGATCCGCGTGCGGGTCTGGAGCTTCGACGAGGCCGAGCCGATCGATCACGCGTTCTTCAAGCGG
CGCATCCAGCGCGCGGTCGCGCACCGCCAGACCATGGTGACCAGCACCGACGCAGTGCGGCTCGTGTTCGGCGAGGCCGA
CGGCCTGCCGGGCCTGATCGTCGACTATTACGTCGACGACGGCCTGCCACGCGAGCTGCCGCCCGGCCAGGCGCCCGAGG
CCACGCCGGCGGCCGGCGCGCGGCGCGGCCAGCTGGTCTGCCAGTTCATGGCGGCCGGCGTCGAGGCCTGGAAGCCGGCC
ATCGTCGCCGCGCTCATCGGCGCGACCGGCTGCCCGAACGTCTACGAGCGCTCGGACGTCTCGATCCGCCAGAAGGAAGG
GCTCGAGCAGACCACCGGCGTGCTGGCGGGCGAGGCGCCGCCCGCCACGCTGGTCACGCGCGAGAACGGCGTGCGCTATC
ACGTCGACGTGTTCAACGGCCACAAGACCGGCTTCTATGTCGACCAGCGCGACAACCGCGCGCTGGTCGGCCAGGTCTCG
AACGGGCGCGACGTGCTGAACTGCTTCTGCTACACGGGCGGCTTCTCGCTGGCGGCGCTGGCCGGCGGCGCGAGCCGCGT
GGTCTCGATCGATTCGTCCGGCGACGCGCTCGCGCTCGCGCAGCGCAACGTGAGCGCCAACGGGCTCGACCCGGCTCGCG
CGAGCTGGCTCGACGCCGACGCGTTCAAGACGCTGCGGCGCCTGGCCGACGAGGGCGAGCGCTTCGACGTGGTGGTGCTC
GACCCGCCGAAGTTCGCGCCGGCGCGCGAGCACGTGGACCGCGCCGCGCGCGCCTATAAGGACATCAACCTGAGCGGCTT
CCGGCTGCTGCGCCCGGGCGGCCTGCTGTTCACCTACTCCTGCTCGGGGGCGATCGACATGGACCTGTTCCAGAAGATCG
TGGCCGGCGCCGCCGCCGACGCGCGCGTCGACGCGCGGATCCTCAGGCGGCTCGGCGCCGGGGTGGACCACCCGCTGCTG
ACCGCGTTCCCGGAGGGCGAATATCTGAAGGGCCTGCTGTTGCAAATCGCCTGA

Upstream 100 bases:

>100_bases
AGAAGCGCGACTGACGGCGCCCCGGCACGCCGCCCCGCCGCCGTCTGCTTCGCGAGCCTTTGTTGCCGCCGCGCCCCGCG
CGGCCCGAACCGATACCGAC

Downstream 100 bases:

>100_bases
GCGCCCCGATCTGAGTCGTTCCATCGCGCCTGCCGGACAGCCGGGGCGCGGGCGCTTGACATCTATGTTTCAATAGGTGG
TTCGGCACGCGCGGCATCCG

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MQTVTLKPSKDKSLLRRHPWIYANAIEHVDGKPAAGATVVVRSHDGRFLARGAYSPHSQIRVRVWSFDEAEPIDHAFFKR
RIQRAVAHRQTMVTSTDAVRLVFGEADGLPGLIVDYYVDDGLPRELPPGQAPEATPAAGARRGQLVCQFMAAGVEAWKPA
IVAALIGATGCPNVYERSDVSIRQKEGLEQTTGVLAGEAPPATLVTRENGVRYHVDVFNGHKTGFYVDQRDNRALVGQVS
NGRDVLNCFCYTGGFSLAALAGGASRVVSIDSSGDALALAQRNVSANGLDPARASWLDADAFKTLRRLADEGERFDVVVL
DPPKFAPAREHVDRAARAYKDINLSGFRLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADARVDARILRRLGAGVDHPLL
TAFPEGEYLKGLLLQIA

Sequences:

>Translated_417_residues
MQTVTLKPSKDKSLLRRHPWIYANAIEHVDGKPAAGATVVVRSHDGRFLARGAYSPHSQIRVRVWSFDEAEPIDHAFFKR
RIQRAVAHRQTMVTSTDAVRLVFGEADGLPGLIVDYYVDDGLPRELPPGQAPEATPAAGARRGQLVCQFMAAGVEAWKPA
IVAALIGATGCPNVYERSDVSIRQKEGLEQTTGVLAGEAPPATLVTRENGVRYHVDVFNGHKTGFYVDQRDNRALVGQVS
NGRDVLNCFCYTGGFSLAALAGGASRVVSIDSSGDALALAQRNVSANGLDPARASWLDADAFKTLRRLADEGERFDVVVL
DPPKFAPAREHVDRAARAYKDINLSGFRLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADARVDARILRRLGAGVDHPLL
TAFPEGEYLKGLLLQIA
>Mature_417_residues
MQTVTLKPSKDKSLLRRHPWIYANAIEHVDGKPAAGATVVVRSHDGRFLARGAYSPHSQIRVRVWSFDEAEPIDHAFFKR
RIQRAVAHRQTMVTSTDAVRLVFGEADGLPGLIVDYYVDDGLPRELPPGQAPEATPAAGARRGQLVCQFMAAGVEAWKPA
IVAALIGATGCPNVYERSDVSIRQKEGLEQTTGVLAGEAPPATLVTRENGVRYHVDVFNGHKTGFYVDQRDNRALVGQVS
NGRDVLNCFCYTGGFSLAALAGGASRVVSIDSSGDALALAQRNVSANGLDPARASWLDADAFKTLRRLADEGERFDVVVL
DPPKFAPAREHVDRAARAYKDINLSGFRLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADARVDARILRRLGAGVDHPLL
TAFPEGEYLKGLLLQIA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=415, Percent_Identity=44.0963855421687, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1787181, Length=173, Percent_Identity=32.3699421965318, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45095; Mature: 45095

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVTLKPSKDKSLLRRHPWIYANAIEHVDGKPAAGATVVVRSHDGRFLARGAYSPHSQI
CCEEECCCCCCHHHHHHCCCEEEEHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCEE
RVRVWSFDEAEPIDHAFFKRRIQRAVAHRQTMVTSTDAVRLVFGEADGLPGLIVDYYVDD
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCEEEEEEECC
GLPRELPPGQAPEATPAAGARRGQLVCQFMAAGVEAWKPAIVAALIGATGCPNVYERSDV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
SIRQKEGLEQTTGVLAGEAPPATLVTRENGVRYHVDVFNGHKTGFYVDQRDNRALVGQVS
CCHHHCCCHHHHCEEECCCCCCEEEECCCCCEEEEEEECCCCCCEEEECCCCCEEEEECC
NGRDVLNCFCYTGGFSLAALAGGASRVVSIDSSGDALALAQRNVSANGLDPARASWLDAD
CCCCEEEEEEECCCCCEEHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHCCHH
AFKTLRRLADEGERFDVVVLDPPKFAPAREHVDRAARAYKDINLSGFRLLRPGGLLFTYS
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEE
CSGAIDMDLFQKIVAGAAADARVDARILRRLGAGVDHPLLTAFPEGEYLKGLLLQIA
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHCCC
>Mature Secondary Structure
MQTVTLKPSKDKSLLRRHPWIYANAIEHVDGKPAAGATVVVRSHDGRFLARGAYSPHSQI
CCEEECCCCCCHHHHHHCCCEEEEHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCEE
RVRVWSFDEAEPIDHAFFKRRIQRAVAHRQTMVTSTDAVRLVFGEADGLPGLIVDYYVDD
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCEEEEEEECC
GLPRELPPGQAPEATPAAGARRGQLVCQFMAAGVEAWKPAIVAALIGATGCPNVYERSDV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
SIRQKEGLEQTTGVLAGEAPPATLVTRENGVRYHVDVFNGHKTGFYVDQRDNRALVGQVS
CCHHHCCCHHHHCEEECCCCCCEEEECCCCCEEEEEEECCCCCCEEEECCCCCEEEEECC
NGRDVLNCFCYTGGFSLAALAGGASRVVSIDSSGDALALAQRNVSANGLDPARASWLDAD
CCCCEEEEEEECCCCCEEHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHCCHH
AFKTLRRLADEGERFDVVVLDPPKFAPAREHVDRAARAYKDINLSGFRLLRPGGLLFTYS
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEE
CSGAIDMDLFQKIVAGAAADARVDARILRRLGAGVDHPLLTAFPEGEYLKGLLLQIA
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA