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The map label for this gene is oprM [H]

Identifier: 238028826

GI number: 238028826

Start: 3745906

End: 3747429

Strand: Direct

Name: oprM [H]

Synonym: bglu_1g32910

Alternate gene names: 238028826

Gene position: 3745906-3747429 (Clockwise)

Preceding gene: 339905980

Following gene: 238028829

Centisome position: 95.89

GC content: 76.71

Gene sequence:

>1524_bases
ATGGCTAAGCGCCGGCTCTGCGCGACGCTCGCGAGCGTCACGCTTCTCTGCGCGTGCTCGTTCGCGCCCACCTATCGCGT
GCCCGCCACCGCGCTGCCCGCCGCGTTCAAGGAAGCGGGCGGCTGGCAGCGCGCGCGGCCCGCCGACCGCATCGCGCGCG
ACGGCTGGTGGCGCGCGTTTCGCGACCCCGTGCTCGACCGGCTGGAAGCGCAGGTGGCACGCGCCAACCCCGACGTGGCC
GCCGCCGTGGCTCGCCACGACGAGGCCGACGCGCTGCTCGATCAGGCGCGCGCGGGGCTGTTCCCGACGCTCGGGCTCGA
CACCCAGGTATCCAGCAACCGCGCCTCGGCCACCCGCCCGCTGCGCGGCGCGACCCAGCCCGACGTCTACGGCGCCAACA
CGCTCGAGGCCGGCTTCGACTACGACCTCGATCTCTGGGGCAAGCTGCGCAACGAGGTGGCGGCCGGCCGCGCCGACGCG
GAGGCCAGCGCCGACGATCTGGCCTCGGTGCGGCTGAGCCTGCAGGCCAGCCTCGCGCGCGCCTACTTCAGGCTGCGCGG
GCTCGACCAGCAGCAGCGCATGCTGGCCGACACGATCGACACCTACCGGCGCGCGCTGCAGCTGACGCTGAGCCGCCATG
CCGGCGGCATCGCCTCCGACCTCGACGTCTCGCGCGCGCAGACCCGGCTCGACGCGGCGCGCGCCGCGGCCGAGGACGTG
AAGGCGCGCCGCGCGCTCTACGAGCACGCGGTGGCCACCCTCACGGGCGTGCCGGCCTCGTCGTTCTCGCTGCCGGCGAG
CCCCGGCGCGGCGCGCCTGCCGTCGATCCCGGCCGGCATCCCGGCCGCGCTGCTGCAGCGCCGCCCCGACGTGGCCGCCG
CCGAGCGGCGCGTGGCGGCCGCCAACGCGCGGATCGGCGTGGCGCGGGCCGCGTTCTTCCCCGACCTCACGCTCGGGCTG
GCCGGCGGCTACCAAAGCTCGGGGCTCGGCCGCTGGCTCAGCGCGCCGAACGAGATCTGGTCGATCGGGCCGAGCCTGAT
GCTGACGCTGTTCGACGGCGGGCGCCGCCAGGCGATCAGCGACGAGGCGCGCGCCAGGCTCGCCGAGAACGGCGCGCGGT
ATCGCTCGGTGGTGCTGCGCGCCTGCCAGCAGGTGGAGGACGCGCTGGCGCTCACGCACCACCTCGGCGACGAGGCCCGC
CGCGAGCAGGAAGCGCTCGACGCGGCCGAGCGCACGCTGCGGCTGTCCAGCTCGCGCTATCGCGACGGCGTGGTCAGCTA
TCTCGACGTGGTGACCGCGCAGGCCACCGAGCTCGACACCAGGATCGCGATGATCGAACTCGACACGCGGCGCCAGCTGG
CGGCCGTCGCGCTGATCGCGGCGCTGGGCGGCGGCTGGCGCGCGGCGGATGCCGGGCACGACGGCGCGGCTCCGGCTCCG
GCTCCGGCTCCGGCCCCGGCTCCGGCCGTGGCCGTGCCGGTCTCGGCGGCGCTGCCGGCCGGCGCGGCGGGCCATGCGGG
CTGA

Upstream 100 bases:

>100_bases
CCGCCCGACTCGCTCGCCTCGGGCGATCCGGTCCGGCTCGCCGCGCCCGCCGCCACGCTCGCCTCGGCCAGGCCCGCGGC
ATCGCAGACGGAGCAATCGC

Downstream 100 bases:

>100_bases
GCGCGTCGCGCGGCCGGTCCGCGATACCGGTCCGCGATACCGGTCCGCGGTGCCGGCGCGATGCCGGCCGCCTTGCGCGG
CGCCGCGCCGGGCGCCGGCC

Product: NodT family RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MAKRRLCATLASVTLLCACSFAPTYRVPATALPAAFKEAGGWQRARPADRIARDGWWRAFRDPVLDRLEAQVARANPDVA
AAVARHDEADALLDQARAGLFPTLGLDTQVSSNRASATRPLRGATQPDVYGANTLEAGFDYDLDLWGKLRNEVAAGRADA
EASADDLASVRLSLQASLARAYFRLRGLDQQQRMLADTIDTYRRALQLTLSRHAGGIASDLDVSRAQTRLDAARAAAEDV
KARRALYEHAVATLTGVPASSFSLPASPGAARLPSIPAGIPAALLQRRPDVAAAERRVAAANARIGVARAAFFPDLTLGL
AGGYQSSGLGRWLSAPNEIWSIGPSLMLTLFDGGRRQAISDEARARLAENGARYRSVVLRACQQVEDALALTHHLGDEAR
REQEALDAAERTLRLSSSRYRDGVVSYLDVVTAQATELDTRIAMIELDTRRQLAAVALIAALGGGWRAADAGHDGAAPAP
APAPAPAPAVAVPVSAALPAGAAGHAG

Sequences:

>Translated_507_residues
MAKRRLCATLASVTLLCACSFAPTYRVPATALPAAFKEAGGWQRARPADRIARDGWWRAFRDPVLDRLEAQVARANPDVA
AAVARHDEADALLDQARAGLFPTLGLDTQVSSNRASATRPLRGATQPDVYGANTLEAGFDYDLDLWGKLRNEVAAGRADA
EASADDLASVRLSLQASLARAYFRLRGLDQQQRMLADTIDTYRRALQLTLSRHAGGIASDLDVSRAQTRLDAARAAAEDV
KARRALYEHAVATLTGVPASSFSLPASPGAARLPSIPAGIPAALLQRRPDVAAAERRVAAANARIGVARAAFFPDLTLGL
AGGYQSSGLGRWLSAPNEIWSIGPSLMLTLFDGGRRQAISDEARARLAENGARYRSVVLRACQQVEDALALTHHLGDEAR
REQEALDAAERTLRLSSSRYRDGVVSYLDVVTAQATELDTRIAMIELDTRRQLAAVALIAALGGGWRAADAGHDGAAPAP
APAPAPAPAVAVPVSAALPAGAAGHAG
>Mature_506_residues
AKRRLCATLASVTLLCACSFAPTYRVPATALPAAFKEAGGWQRARPADRIARDGWWRAFRDPVLDRLEAQVARANPDVAA
AVARHDEADALLDQARAGLFPTLGLDTQVSSNRASATRPLRGATQPDVYGANTLEAGFDYDLDLWGKLRNEVAAGRADAE
ASADDLASVRLSLQASLARAYFRLRGLDQQQRMLADTIDTYRRALQLTLSRHAGGIASDLDVSRAQTRLDAARAAAEDVK
ARRALYEHAVATLTGVPASSFSLPASPGAARLPSIPAGIPAALLQRRPDVAAAERRVAAANARIGVARAAFFPDLTLGLA
GGYQSSGLGRWLSAPNEIWSIGPSLMLTLFDGGRRQAISDEARARLAENGARYRSVVLRACQQVEDALALTHHLGDEARR
EQEALDAAERTLRLSSSRYRDGVVSYLDVVTAQATELDTRIAMIELDTRRQLAAVALIAALGGGWRAADAGHDGAAPAPA
PAPAPAPAVAVPVSAALPAGAAGHAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=29.247311827957, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI2367348, Length=489, Percent_Identity=28.6298568507157, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53525; Mature: 53394

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRRLCATLASVTLLCACSFAPTYRVPATALPAAFKEAGGWQRARPADRIARDGWWRAF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RDPVLDRLEAQVARANPDVAAAVARHDEADALLDQARAGLFPTLGLDTQVSSNRASATRP
HHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LRGATQPDVYGANTLEAGFDYDLDLWGKLRNEVAAGRADAEASADDLASVRLSLQASLAR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AYFRLRGLDQQQRMLADTIDTYRRALQLTLSRHAGGIASDLDVSRAQTRLDAARAAAEDV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
KARRALYEHAVATLTGVPASSFSLPASPGAARLPSIPAGIPAALLQRRPDVAAAERRVAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHH
ANARIGVARAAFFPDLTLGLAGGYQSSGLGRWLSAPNEIWSIGPSLMLTLFDGGRRQAIS
HCCHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHCCCHHHHHCCHHHEEEEECCCCCHHHH
DEARARLAENGARYRSVVLRACQQVEDALALTHHLGDEARREQEALDAAERTLRLSSSRY
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RDGVVSYLDVVTAQATELDTRIAMIELDTRRQLAAVALIAALGGGWRAADAGHDGAAPAP
HHHHHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCC
APAPAPAPAVAVPVSAALPAGAAGHAG
CCCCCCCCCEECCHHHHCCCCCCCCCC
>Mature Secondary Structure 
AKRRLCATLASVTLLCACSFAPTYRVPATALPAAFKEAGGWQRARPADRIARDGWWRAF
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RDPVLDRLEAQVARANPDVAAAVARHDEADALLDQARAGLFPTLGLDTQVSSNRASATRP
HHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LRGATQPDVYGANTLEAGFDYDLDLWGKLRNEVAAGRADAEASADDLASVRLSLQASLAR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AYFRLRGLDQQQRMLADTIDTYRRALQLTLSRHAGGIASDLDVSRAQTRLDAARAAAEDV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
KARRALYEHAVATLTGVPASSFSLPASPGAARLPSIPAGIPAALLQRRPDVAAAERRVAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHH
ANARIGVARAAFFPDLTLGLAGGYQSSGLGRWLSAPNEIWSIGPSLMLTLFDGGRRQAIS
HCCHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHCCCHHHHHCCHHHEEEEECCCCCHHHH
DEARARLAENGARYRSVVLRACQQVEDALALTHHLGDEARREQEALDAAERTLRLSSSRY
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RDGVVSYLDVVTAQATELDTRIAMIELDTRRQLAAVALIAALGGGWRAADAGHDGAAPAP
HHHHHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCC
APAPAPAPAVAVPVSAALPAGAAGHAG
CCCCCCCCCEECCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]