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The map label for this gene is paaF [C]

Identifier: 238028759

GI number: 238028759

Start: 3659672

End: 3660502

Strand: Reverse

Name: paaF [C]

Synonym: bglu_1g32230

Alternate gene names: 238028759

Gene position: 3660502-3659672 (Counterclockwise)

Preceding gene: 238028760

Following gene: 238028758

Centisome position: 93.7

GC content: 72.44

Gene sequence:

>831_bases
ATGAGCCAACCCGATGCCTACGCCGGCTACCAGGCGCTGCGGCTGCGGCGCCTGCCGCACGGCATCCTCGAGATCGTGAT
GAGCGGCGAGGGCGCCAACCGCAGCGGCCTCGCCACCGCCGACGCGCGCATGCACCGCGAGCTCGCCGAAATCTGGCGCG
ACCTCGACCGCGATCCCGAGGTGCGCGTCGCGCTGATCCGCGGCGAGGGCAAGGGCTTCTCGGCGGGCGGCGATCTCGCT
CTCGTCGAGCAGATGGCCGACGATTTCGCGGTGCGCACGCGCGTCTGGCACGAGGCGCGCGACCTCGTCTACAACGTGAT
CAACTGCGGCAAGCCGATCGTCTCGGCGATGCACGGGCCGGCGGTGGGCGCGGGGCTGGTGGCCGGGCTGCTGGCCGACG
TGTCGATCGCCGCGAACGACGCGCGCATCATCGACGGCCATACGCGGCTCGGGGTGGCGGCCGGCGACCACGCCGCGATC
GTCTGGCCGCTGTTGTGCGGCATGGCGAAGGCCAAGTATCACCTGCTGCTCTGCGAGCCGGTGAGCGGGGCGCAGGCCGA
GCGCATCGGGCTCGTGTCGCTGGCCGTCGACGCGGCCGAGCTGCTGCCGAAGGCGCTGGAGCTGGCCGAGCGGCTCGCGC
ACGGTTCGCAGAGCGCGATTCGCTGGACCAAGTACGCGCTCAACAACTGGCTGCGCATGGCCGGGCCGACCTTCGACGCG
TCGCTCGCGCTCGAATTCATGGGCTTCTCGGGTCCGGACGTGCGCGAGGGCATCGACTCGCTGCGCGCGCGGCGCGCGCC
CGACTTCGACGGCGCGGCCGGCAAGCCGTGA

Upstream 100 bases:

>100_bases
AGCAAGCCGTCGCGCCGCTCGAGGCGGCGTCCTGAACACGCGCGGCGCCGCTGCCGAGGCACCCGCACGACACCATCATG
ACCCTGACAGGGAGCGAGAC

Downstream 100 bases:

>100_bases
GGGCGCGGCCGCGGGGCGCGGAGGCTGCCGGCCGCCGCGCCGCTCGCCGGCATCCCGCGCCCGCTATCCGCCGCCCATGC
GCCCTCGGCCGCATCGCCGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSQPDAYAGYQALRLRRLPHGILEIVMSGEGANRSGLATADARMHRELAEIWRDLDRDPEVRVALIRGEGKGFSAGGDLA
LVEQMADDFAVRTRVWHEARDLVYNVINCGKPIVSAMHGPAVGAGLVAGLLADVSIAANDARIIDGHTRLGVAAGDHAAI
VWPLLCGMAKAKYHLLLCEPVSGAQAERIGLVSLAVDAAELLPKALELAERLAHGSQSAIRWTKYALNNWLRMAGPTFDA
SLALEFMGFSGPDVREGIDSLRARRAPDFDGAAGKP

Sequences:

>Translated_276_residues
MSQPDAYAGYQALRLRRLPHGILEIVMSGEGANRSGLATADARMHRELAEIWRDLDRDPEVRVALIRGEGKGFSAGGDLA
LVEQMADDFAVRTRVWHEARDLVYNVINCGKPIVSAMHGPAVGAGLVAGLLADVSIAANDARIIDGHTRLGVAAGDHAAI
VWPLLCGMAKAKYHLLLCEPVSGAQAERIGLVSLAVDAAELLPKALELAERLAHGSQSAIRWTKYALNNWLRMAGPTFDA
SLALEFMGFSGPDVREGIDSLRARRAPDFDGAAGKP
>Mature_275_residues
SQPDAYAGYQALRLRRLPHGILEIVMSGEGANRSGLATADARMHRELAEIWRDLDRDPEVRVALIRGEGKGFSAGGDLAL
VEQMADDFAVRTRVWHEARDLVYNVINCGKPIVSAMHGPAVGAGLVAGLLADVSIAANDARIIDGHTRLGVAAGDHAAIV
WPLLCGMAKAKYHLLLCEPVSGAQAERIGLVSLAVDAAELLPKALELAERLAHGSQSAIRWTKYALNNWLRMAGPTFDAS
LALEFMGFSGPDVREGIDSLRARRAPDFDGAAGKP

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI1787659, Length=224, Percent_Identity=32.1428571428571, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787660, Length=243, Percent_Identity=30.8641975308642, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI221142681, Length=208, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788597, Length=275, Percent_Identity=24, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=232, Percent_Identity=28.448275862069, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=183, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=236, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=238, Percent_Identity=24.7899159663866, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24654903, Length=157, Percent_Identity=27.3885350318471, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=26.2948207171315, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20129971, Length=234, Percent_Identity=24.3589743589744, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653477, Length=234, Percent_Identity=24.3589743589744, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29518; Mature: 29387

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPDAYAGYQALRLRRLPHGILEIVMSGEGANRSGLATADARMHRELAEIWRDLDRDPE
CCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VRVALIRGEGKGFSAGGDLALVEQMADDFAVRTRVWHEARDLVYNVINCGKPIVSAMHGP
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
AVGAGLVAGLLADVSIAANDARIIDGHTRLGVAAGDHAAIVWPLLCGMAKAKYHLLLCEP
HHHHHHHHHHHHHHHEECCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECC
VSGAQAERIGLVSLAVDAAELLPKALELAERLAHGSQSAIRWTKYALNNWLRMAGPTFDA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCH
SLALEFMGFSGPDVREGIDSLRARRAPDFDGAAGKP
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SQPDAYAGYQALRLRRLPHGILEIVMSGEGANRSGLATADARMHRELAEIWRDLDRDPE
CCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VRVALIRGEGKGFSAGGDLALVEQMADDFAVRTRVWHEARDLVYNVINCGKPIVSAMHGP
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
AVGAGLVAGLLADVSIAANDARIIDGHTRLGVAAGDHAAIVWPLLCGMAKAKYHLLLCEP
HHHHHHHHHHHHHHHEECCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECC
VSGAQAERIGLVSLAVDAAELLPKALELAERLAHGSQSAIRWTKYALNNWLRMAGPTFDA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCH
SLALEFMGFSGPDVREGIDSLRARRAPDFDGAAGKP
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA