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The map label for this gene is phbI [H]

Identifier: 238028718

GI number: 238028718

Start: 3613628

End: 3615400

Strand: Reverse

Name: phbI [H]

Synonym: bglu_1g31820

Alternate gene names: 238028718

Gene position: 3615400-3613628 (Counterclockwise)

Preceding gene: 238028719

Following gene: 238028714

Centisome position: 92.55

GC content: 68.7

Gene sequence:

>1773_bases
GTGTCGTTCACGCTGCAGGGAATTCCCGTTTCGAGAGGTATCGCGATCGGTCGTGCGTATCTGATCGCGCCGGCGGCGCT
CGACGTCGCGCATTACCTCGTCGAGGCGCAGCAGCTCGACGCCGAGATCGACCGGCTGCGCGCCGCGCTGCTCGCCGTGC
GCGGCGAACTCGACGCGCTGCGCGCCGACCTGACCGACGACACGCCGAGCGAGGTGGCCGCGTTCATCGACGTGCATTCG
ATGATCCTCAACGACGCGATGCTGGTGCAGGAAACCATCGACCTGATCCGCGACCGCCGCTACAACGCCGAATGGGCGCT
GACCGAGCAGCTCGACGTGATCGCCAGCCACTTCGACGACATCGAGGACGAGTACCTGCGCGAGCGCAAGGCCGACATCG
AGCAGGTGGTCGAGCGCGTGCTGAAGGCGCTGGCCGGCGCGCCGTCGCCGGCCCAGGCGCTCGGCCGCGCGGCCAGCGGC
AAGGACGACATGATCGTGGTCGCGCACGACATCGCGCCGGCCGACATGATGCAGTTCAAGAACCAGGCGTTCCACGCGTT
CGTGACCGATCTGGGCGGGCGCACCTCGCACACGGCGATCGTCGCCCGCAGCCTGGGCATCCCGGCCGCGGTCGGCGTGC
AGCATGCGAGCGCGCTGATCCGCCAGAACGACCTGATCATCGTCGATGGCGACCAGGGCATCGTGATCGTCGATCCGGCG
CCGATCGTGCTCGAGGAATACTCGTACCGGCAGTCCGAGAAGGCGCTCGAGCAGCGCAAGCTGCAGCGCCTCAAGTTCTC
GCCGACGCAGACGCTCTGCGGCACCAAGATCGACCTGCTCGCCAACATCGAGCTGCCCGACGACGCGAAGGCGGCCGTGG
AGGCCGGCGCGGTCGGCGTGGGTCTGTTCCGCACCGAATTCCTGTTCATGAACGACGAGAACGCGCTCGCCGAGGAAGAG
GAGCAGTTCCGCGCGTACCGCCGTGCGGTCGAGCTGATGAACGGCATGCCGGTGACGATCCGCACCATCGACGTGGGTGC
CGACAAGCCGCTCGACGCGCTCGACGGCGGCTACGAGACCGCCCCGAATCCGGCGCTGGGCCTGCGCGCGATCCGCTGGA
GCCTGTCCGAGCCGCAGATGTTCCTGACCCAGCTGCGCGCGATCCTGCGCGCCTCGGCGATCGGGCCGGTGAAGATCCTG
GTGCCGATGCTCGCGCACGCGCAGGAGATCGACCAGACGCTCGACCTGATCCGCGAGGCCAAGCGCCAGCTCGACGACGC
CGGGCTCGCCTACGATCCGAACGTGCGGGTCGGCGCGATGATCGAGATCCCGGCCGCCGCGATCGCGCTGCCGCTGTTCC
TCAAGCGCGTCGATTTCCTCTCGATCGGCACCAACGACCTGATCCAGTACACGCTCGCGATCGACCGCGCCGACAATGCC
GTGGCGCATCTGTACGATCCGCTGCACCCGGCCGTGCTGCACCTGATCGCCTACACGCTGCGCGAGGCCAAGCGCGCGGG
CGTGCCGGTGTCGGTGTGCGGTGAGATGGCCGGCGATCCGGCGATGACGCGCCTGCTGCTCGGCATGGGGCTCACCGAGT
TCTCGATGCATCCGAGCCAGCTGCTGGTGGTCAAGCAGGAGATCCTGCGCGCGCACCTGAAGGCGCTCGAGAAGCCGACC
GCCGACGTGCTGGCCTCGTTCGAGCCCGAGGACGTGCAGCTCGCGCTGAAGCAGCTCGCGCAGGCCGAGCCGAAGGCCGA
CCTGGTGGCCTGA

Upstream 100 bases:

>100_bases
ATGCAGCCACGCGGGGAGCGCGGAATTTATAATCACGAGTCGGGGTCATATGCGCATCGCGGCGGTGGGCTGCGGCTGAC
CTGACTCACAGGAGGTGCGC

Downstream 100 bases:

>100_bases
GGCGGCCCGCCCGGGGCCGGCCGCATGCGGCCGGCGCTGCCTCGCCTTGCGCCTCGAACTCGCGGCCTCGACGCGGCGGC
CGGCGGCCCGCCGGGTCTTT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MSFTLQGIPVSRGIAIGRAYLIAPAALDVAHYLVEAQQLDAEIDRLRAALLAVRGELDALRADLTDDTPSEVAAFIDVHS
MILNDAMLVQETIDLIRDRRYNAEWALTEQLDVIASHFDDIEDEYLRERKADIEQVVERVLKALAGAPSPAQALGRAASG
KDDMIVVAHDIAPADMMQFKNQAFHAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQNDLIIVDGDQGIVIVDPA
PIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTKIDLLANIELPDDAKAAVEAGAVGVGLFRTEFLFMNDENALAEEE
EQFRAYRRAVELMNGMPVTIRTIDVGADKPLDALDGGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAIGPVKIL
VPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAIDRADNA
VAHLYDPLHPAVLHLIAYTLREAKRAGVPVSVCGEMAGDPAMTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKALEKPT
ADVLASFEPEDVQLALKQLAQAEPKADLVA

Sequences:

>Translated_590_residues
MSFTLQGIPVSRGIAIGRAYLIAPAALDVAHYLVEAQQLDAEIDRLRAALLAVRGELDALRADLTDDTPSEVAAFIDVHS
MILNDAMLVQETIDLIRDRRYNAEWALTEQLDVIASHFDDIEDEYLRERKADIEQVVERVLKALAGAPSPAQALGRAASG
KDDMIVVAHDIAPADMMQFKNQAFHAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQNDLIIVDGDQGIVIVDPA
PIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTKIDLLANIELPDDAKAAVEAGAVGVGLFRTEFLFMNDENALAEEE
EQFRAYRRAVELMNGMPVTIRTIDVGADKPLDALDGGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAIGPVKIL
VPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAIDRADNA
VAHLYDPLHPAVLHLIAYTLREAKRAGVPVSVCGEMAGDPAMTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKALEKPT
ADVLASFEPEDVQLALKQLAQAEPKADLVA
>Mature_589_residues
SFTLQGIPVSRGIAIGRAYLIAPAALDVAHYLVEAQQLDAEIDRLRAALLAVRGELDALRADLTDDTPSEVAAFIDVHSM
ILNDAMLVQETIDLIRDRRYNAEWALTEQLDVIASHFDDIEDEYLRERKADIEQVVERVLKALAGAPSPAQALGRAASGK
DDMIVVAHDIAPADMMQFKNQAFHAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQNDLIIVDGDQGIVIVDPAP
IVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTKIDLLANIELPDDAKAAVEAGAVGVGLFRTEFLFMNDENALAEEEE
QFRAYRRAVELMNGMPVTIRTIDVGADKPLDALDGGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAIGPVKILV
PMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAIDRADNAV
AHLYDPLHPAVLHLIAYTLREAKRAGVPVSVCGEMAGDPAMTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKALEKPTA
DVLASFEPEDVQLALKQLAQAEPKADLVA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=37.6532399299475, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1789193, Length=537, Percent_Identity=35.0093109869646, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI48994992, Length=529, Percent_Identity=31.758034026465, Blast_Score=248, Evalue=9e-67,
Organism=Escherichia coli, GI1788726, Length=545, Percent_Identity=28.9908256880734, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787994, Length=412, Percent_Identity=28.1553398058252, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI226510935, Length=194, Percent_Identity=25.7731958762887, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64379; Mature: 64248

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTLQGIPVSRGIAIGRAYLIAPAALDVAHYLVEAQQLDAEIDRLRAALLAVRGELDAL
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RADLTDDTPSEVAAFIDVHSMILNDAMLVQETIDLIRDRRYNAEWALTEQLDVIASHFDD
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCC
IEDEYLRERKADIEQVVERVLKALAGAPSPAQALGRAASGKDDMIVVAHDIAPADMMQFK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEECCCHHHHHHHH
NQAFHAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQNDLIIVDGDQGIVIVDPA
HHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCC
PIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTKIDLLANIELPDDAKAAVEAGAVGV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEECCCCCCHHHHHHHCCCEE
GLFRTEFLFMNDENALAEEEEQFRAYRRAVELMNGMPVTIRTIDVGADKPLDALDGGYET
EHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCC
APNPALGLRAIRWSLSEPQMFLTQLRAILRASAIGPVKILVPMLAHAQEIDQTLDLIREA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KRQLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAIDRADNA
HHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEHEEECCCCH
VAHLYDPLHPAVLHLIAYTLREAKRAGVPVSVCGEMAGDPAMTRLLLGMGLTEFSMHPSQ
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHH
LLVVKQEILRAHLKALEKPTADVLASFEPEDVQLALKQLAQAEPKADLVA
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SFTLQGIPVSRGIAIGRAYLIAPAALDVAHYLVEAQQLDAEIDRLRAALLAVRGELDAL
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RADLTDDTPSEVAAFIDVHSMILNDAMLVQETIDLIRDRRYNAEWALTEQLDVIASHFDD
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCC
IEDEYLRERKADIEQVVERVLKALAGAPSPAQALGRAASGKDDMIVVAHDIAPADMMQFK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEECCCHHHHHHHH
NQAFHAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQNDLIIVDGDQGIVIVDPA
HHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCC
PIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTKIDLLANIELPDDAKAAVEAGAVGV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEECCCCCCHHHHHHHCCCEE
GLFRTEFLFMNDENALAEEEEQFRAYRRAVELMNGMPVTIRTIDVGADKPLDALDGGYET
EHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCC
APNPALGLRAIRWSLSEPQMFLTQLRAILRASAIGPVKILVPMLAHAQEIDQTLDLIREA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KRQLDDAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAIDRADNA
HHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEHEEECCCCH
VAHLYDPLHPAVLHLIAYTLREAKRAGVPVSVCGEMAGDPAMTRLLLGMGLTEFSMHPSQ
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHH
LLVVKQEILRAHLKALEKPTADVLASFEPEDVQLALKQLAQAEPKADLVA
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]