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The map label for this gene is rsmD [H]

Identifier: 238028675

GI number: 238028675

Start: 3566750

End: 3567430

Strand: Reverse

Name: rsmD [H]

Synonym: bglu_1g31390

Alternate gene names: 238028675

Gene position: 3567430-3566750 (Counterclockwise)

Preceding gene: 238028679

Following gene: 238028674

Centisome position: 91.32

GC content: 72.69

Gene sequence:

>681_bases
GTGCGCCTGTTTGCGGCCCCGGGCCGCGCCGATCCACCCGTTTCGTCCCGTTTGCCCCGATCCCGTATGCCTCGTTCGTC
CTCCCCCCGCCTGTCGGCCGGTGCCAGCCGCGGCAAGCCGCACACGATCCGCATCATCGGCGGCGAGTGGAAGCGCACGC
CGCTGCCGGTGCTCGACCTCGACGGGCTGCGCCCGACGCCCGATCGCGTGCGCGAGACGTTGTTCAACTGGCTCGGCCAG
GACCTCCACGGCCGGCGCTGTCTCGACCTGTTCGCCGGCACCGGCGCGCTCGGCTTCGAGGCCGCCTCGCGCGGCGCGGC
GAGCGTCGTGATGGTGGAGCGCAACGCGCGTGCCGCGCAGCAACTGCGTGTGATTCGCGACAAGCTCTCGGCGCGCGCCG
TCGAGGTGGCCGAAGCCGACGCGCTGCGGCTCGCGGCCGGCCTCGCGCCGGCCTCGTTCGACGTGGTGTTCGTCGATCCG
CCGTTCGCCGACGCCGAACTGTTCGAGCGCGCGCTCGCGCTGGCCGTCAAGCTCGTGGCACCCGGCGGCCACCTGTACGT
GGAAGCCGGCGACGCGCTCGACCCGGCCGCCCACGAGGCGCTCGCGGGCTGGACCGTGACGCGCGAGGGCAAGGCCGGCG
CGGTCCGCTATCATTTGCTGCAACGCGAAAATGATGAATAA

Upstream 100 bases:

>100_bases
GGGATCGGCAGCGCGGCGGCCGTGGCCGGCGCCCGAAACGGGGGATTTTATCAGGCGTGCCGCGCGGGCAGCGGCGAAGC
GCTGCGCCGGCTGTGGTAAC

Downstream 100 bases:

>100_bases
TGCGCGTTCCAAACGAGGCGCCGCGCGCAGCGCGCGACGCGCGCCCATCGTGGGGGCGGCCGTGATCCGTATCGCCGCCG
CACCAATCGCATGATCACAG

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MRLFAAPGRADPPVSSRLPRSRMPRSSSPRLSAGASRGKPHTIRIIGGEWKRTPLPVLDLDGLRPTPDRVRETLFNWLGQ
DLHGRRCLDLFAGTGALGFEAASRGAASVVMVERNARAAQQLRVIRDKLSARAVEVAEADALRLAAGLAPASFDVVFVDP
PFADAELFERALALAVKLVAPGGHLYVEAGDALDPAAHEALAGWTVTREGKAGAVRYHLLQRENDE

Sequences:

>Translated_226_residues
MRLFAAPGRADPPVSSRLPRSRMPRSSSPRLSAGASRGKPHTIRIIGGEWKRTPLPVLDLDGLRPTPDRVRETLFNWLGQ
DLHGRRCLDLFAGTGALGFEAASRGAASVVMVERNARAAQQLRVIRDKLSARAVEVAEADALRLAAGLAPASFDVVFVDP
PFADAELFERALALAVKLVAPGGHLYVEAGDALDPAAHEALAGWTVTREGKAGAVRYHLLQRENDE
>Mature_226_residues
MRLFAAPGRADPPVSSRLPRSRMPRSSSPRLSAGASRGKPHTIRIIGGEWKRTPLPVLDLDGLRPTPDRVRETLFNWLGQ
DLHGRRCLDLFAGTGALGFEAASRGAASVVMVERNARAAQQLRVIRDKLSARAVEVAEADALRLAAGLAPASFDVVFVDP
PFADAELFERALALAVKLVAPGGHLYVEAGDALDPAAHEALAGWTVTREGKAGAVRYHLLQRENDE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=185, Percent_Identity=42.7027027027027, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 24323; Mature: 24323

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFAAPGRADPPVSSRLPRSRMPRSSSPRLSAGASRGKPHTIRIIGGEWKRTPLPVLDL
CEEECCCCCCCCCHHHCCCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEC
DGLRPTPDRVRETLFNWLGQDLHGRRCLDLFAGTGALGFEAASRGAASVVMVERNARAAQ
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHCCCCEEEEEEECCHHHHH
QLRVIRDKLSARAVEVAEADALRLAAGLAPASFDVVFVDPPFADAELFERALALAVKLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHEEC
PGGHLYVEAGDALDPAAHEALAGWTVTREGKAGAVRYHLLQRENDE
CCCEEEEEECCCCCHHHHHHHHCCEEECCCCCCHHEEEEECCCCCC
>Mature Secondary Structure
MRLFAAPGRADPPVSSRLPRSRMPRSSSPRLSAGASRGKPHTIRIIGGEWKRTPLPVLDL
CEEECCCCCCCCCHHHCCCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEC
DGLRPTPDRVRETLFNWLGQDLHGRRCLDLFAGTGALGFEAASRGAASVVMVERNARAAQ
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHCCCCEEEEEEECCHHHHH
QLRVIRDKLSARAVEVAEADALRLAAGLAPASFDVVFVDPPFADAELFERALALAVKLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHEEC
PGGHLYVEAGDALDPAAHEALAGWTVTREGKAGAVRYHLLQRENDE
CCCEEEEEECCCCCHHHHHHHHCCEEECCCCCCHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]