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The map label for this gene is ptsN [H]

Identifier: 238028659

GI number: 238028659

Start: 3551489

End: 3552001

Strand: Direct

Name: ptsN [H]

Synonym: bglu_1g31230

Alternate gene names: 238028659

Gene position: 3551489-3552001 (Clockwise)

Preceding gene: 238028658

Following gene: 238028660

Centisome position: 90.91

GC content: 64.33

Gene sequence:

>513_bases
ATGGAATATCCGTCCACCGCCGCGAGGAGACCCCAGGCTGCCCCCTTGCCTGTCAACATGAATCGCCTAGCCAAAATCCT
GCCACTCGAGAACGTCGTCATCGACCTCTCCGTCACCAGCAAGAAGCGCGTGTTCGAACAAGCCGGCCTGATCTTCGAAA
ATCAGAACGGCATCGCGCGCAGCATCGTCACCGACAACCTGTTCGCGCGCGAGCGTCTCGGCTCGACGGGTCTCGGCGAA
GGCGTCGCCATTCCGCATGGCCGGATCAAGGGCCTCAAGCAGCCGCTCGCCGGGTTCGTCCGGCTCGCCGAGCCGATCCC
GTTCGAGGCGCCGGACGGCCAGCCCGTCTCGCTGCTCATCTTCCTGCTGGTTCCCGAGCAGGCGACGCAGCAGCACCTCG
AGATCCTCTCCGAGATCGCGCAACTGCTGTCCGACCGCGAGGCCCGCGAGCGCCTGCATACCGAGCCGGATCGCAACGAG
TTGCATCAGCTCCTCACTCAATGGCAACCTTGA

Upstream 100 bases:

>100_bases
GTGCACTATAATGTGCGGGTTATTACCGGGGGGCATCGGCGGGGCCGGCTTGTCGTGCACACAAGCCGCCATCGGCCGGC
GCCCCCGCATCTTTGCCGTC

Downstream 100 bases:

>100_bases
ACTGATTGGGGCGGGCAGCCCCCGCATCGGCCTGCAGGCGCAGGCCGGCCCGCCCGCAGCCGGACCGGCGCCGCGACCGC
CGTTGCCGCCCGCCGCATCG

Product: PTS system, nitrogen regulatory IIA protein

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MEYPSTAARRPQAAPLPVNMNRLAKILPLENVVIDLSVTSKKRVFEQAGLIFENQNGIARSIVTDNLFARERLGSTGLGE
GVAIPHGRIKGLKQPLAGFVRLAEPIPFEAPDGQPVSLLIFLLVPEQATQQHLEILSEIAQLLSDREARERLHTEPDRNE
LHQLLTQWQP

Sequences:

>Translated_170_residues
MEYPSTAARRPQAAPLPVNMNRLAKILPLENVVIDLSVTSKKRVFEQAGLIFENQNGIARSIVTDNLFARERLGSTGLGE
GVAIPHGRIKGLKQPLAGFVRLAEPIPFEAPDGQPVSLLIFLLVPEQATQQHLEILSEIAQLLSDREARERLHTEPDRNE
LHQLLTQWQP
>Mature_170_residues
MEYPSTAARRPQAAPLPVNMNRLAKILPLENVVIDLSVTSKKRVFEQAGLIFENQNGIARSIVTDNLFARERLGSTGLGE
GVAIPHGRIKGLKQPLAGFVRLAEPIPFEAPDGQPVSLLIFLLVPEQATQQHLEILSEIAQLLSDREARERLHTEPDRNE
LHQLLTQWQP

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=149, Percent_Identity=36.241610738255, Blast_Score=95, Evalue=2e-21,
Organism=Escherichia coli, GI2367327, Length=119, Percent_Identity=33.6134453781513, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1786951, Length=99, Percent_Identity=34.3434343434343, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 18934; Mature: 18934

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYPSTAARRPQAAPLPVNMNRLAKILPLENVVIDLSVTSKKRVFEQAGLIFENQNGIAR
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCEEECCCCHHH
SIVTDNLFARERLGSTGLGEGVAIPHGRIKGLKQPLAGFVRLAEPIPFEAPDGQPVSLLI
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEH
FLLVPEQATQQHLEILSEIAQLLSDREARERLHTEPDRNELHQLLTQWQP
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MEYPSTAARRPQAAPLPVNMNRLAKILPLENVVIDLSVTSKKRVFEQAGLIFENQNGIAR
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCEEECCCCHHH
SIVTDNLFARERLGSTGLGEGVAIPHGRIKGLKQPLAGFVRLAEPIPFEAPDGQPVSLLI
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEH
FLLVPEQATQQHLEILSEIAQLLSDREARERLHTEPDRNELHQLLTQWQP
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]