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The map label for this gene is rluE [H]

Identifier: 238028429

GI number: 238028429

Start: 3279878

End: 3280456

Strand: Direct

Name: rluE [H]

Synonym: bglu_1g28930

Alternate gene names: 238028429

Gene position: 3279878-3280456 (Clockwise)

Preceding gene: 238028426

Following gene: 238028430

Centisome position: 83.96

GC content: 72.54

Gene sequence:

>579_bases
ATGCCTCTGGTCGCCCTCAACAAACCGTTCGGCACGATCTGCCAGTTCTCGCCGCACGAAACCCGGCCTTCGCTCGGCGA
CTGGGTACGCGCCCCGGGCGTCTATCCGGCTGGCCGGCTCGACGCGGACAGCGAGGGCCTGCTGCTGCTGACCGACGACG
GCGCGCTGCAGGCGCGCATCGCCGAGCCGCGCCACAAGCTCGTCAAGCGCTACTGGGCCCAGGTCGAGGGCGCACCCGAC
GCGGCCGCACTGAAGGCGCTCGCGCGCGGCGTCGACCTCGGCGACTACGTCACGCGCCCGTGCCGCGCGTCGTTGGTCGA
GCCGCCCGCCACGCTCTGGCCGCGTACCCCGCCGGTGCGCTTTCGCGCTGCGATCCCCACCACCTGGATCGAACTCGCGA
TCAGCGAAGGCAAGAACCGCCAGGTGCGCCGGATGACGGCCGCGGTCGGTTTTCCGACGCTGCGGCTGGTGCGCGTCGGC
ATCGGCGCGCTCGACCTGTTCGCGCTCGGCCTCGCGCCCGGCGAGACCATCGAGCTGCCGCCGCGCGCGCCCTGGGACGG
TTTTGCGCCGATCGAATGA

Upstream 100 bases:

>100_bases
CATCAAGTCTTATATAAGACAAAAGACCTGCCGTTCCGTATTATGCATTAAGATTTCGCCATTTGCCATACGACGCGGCG
CGAGCCGCGCAGCCCGCCCG

Downstream 100 bases:

>100_bases
AAGTTTCATGACTCGCGTCAACCAATCGACTCGACCGTCCGTTATTTCACGAAGATGCCGACCGAAGCTATCCGGCATTG
GCGCCAAGCCGCGAAACCGG

Product: Pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MPLVALNKPFGTICQFSPHETRPSLGDWVRAPGVYPAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPD
AAALKALARGVDLGDYVTRPCRASLVEPPATLWPRTPPVRFRAAIPTTWIELAISEGKNRQVRRMTAAVGFPTLRLVRVG
IGALDLFALGLAPGETIELPPRAPWDGFAPIE

Sequences:

>Translated_192_residues
MPLVALNKPFGTICQFSPHETRPSLGDWVRAPGVYPAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPD
AAALKALARGVDLGDYVTRPCRASLVEPPATLWPRTPPVRFRAAIPTTWIELAISEGKNRQVRRMTAAVGFPTLRLVRVG
IGALDLFALGLAPGETIELPPRAPWDGFAPIE
>Mature_191_residues
PLVALNKPFGTICQFSPHETRPSLGDWVRAPGVYPAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPDA
AALKALARGVDLGDYVTRPCRASLVEPPATLWPRTPPVRFRAAIPTTWIELAISEGKNRQVRRMTAAVGFPTLRLVRVGI
GALDLFALGLAPGETIELPPRAPWDGFAPIE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=173, Percent_Identity=52.0231213872832, Blast_Score=176, Evalue=9e-46,
Organism=Escherichia coli, GI1788510, Length=172, Percent_Identity=37.7906976744186, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1787524, Length=170, Percent_Identity=34.1176470588235, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790453, Length=193, Percent_Identity=27.979274611399, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20844; Mature: 20713

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVALNKPFGTICQFSPHETRPSLGDWVRAPGVYPAGRLDADSEGLLLLTDDGALQARI
CCEEECCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEHH
AEPRHKLVKRYWAQVEGAPDAAALKALARGVDLGDYVTRPCRASLVEPPATLWPRTPPVR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHCCCHHHHCCCCCHHHCCCCCCCE
FRAAIPTTWIELAISEGKNRQVRRMTAAVGFPTLRLVRVGIGALDLFALGLAPGETIELP
EEECCCHHEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCEEECC
PRAPWDGFAPIE
CCCCCCCCCCCC
>Mature Secondary Structure 
PLVALNKPFGTICQFSPHETRPSLGDWVRAPGVYPAGRLDADSEGLLLLTDDGALQARI
CEEECCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEHH
AEPRHKLVKRYWAQVEGAPDAAALKALARGVDLGDYVTRPCRASLVEPPATLWPRTPPVR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHCCCHHHHCCCCCHHHCCCCCCCE
FRAAIPTTWIELAISEGKNRQVRRMTAAVGFPTLRLVRVGIGALDLFALGLAPGETIELP
EEECCCHHEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCEEECC
PRAPWDGFAPIE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]